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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= epV30114
         (516 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g03650.1 68418.m00324 1,4-alpha-glucan branching enzyme / sta...   114   4e-26
At2g36390.1 68415.m04466 1,4-alpha-glucan branching enzyme / sta...   107   4e-24
At3g20440.1 68416.m02588 glycoside hydrolase family 13 protein s...    44   5e-05
At3g06290.1 68416.m00722 SAC3/GANP family protein contains Pfam ...    31   0.46 
At2g21710.1 68415.m02582 mitochondrial transcription termination...    29   1.4  
At3g51770.1 68416.m05677 tetratricopeptide repeat (TPR)-containi...    28   3.2  
At3g28960.1 68416.m03620 amino acid transporter family protein l...    27   5.7  
At2g27980.1 68415.m03391 expressed protein                             27   5.7  
At1g25530.1 68414.m03170 lysine and histidine specific transport...    27   5.7  
At2g40810.2 68415.m05035 WD-40 repeat protein family similar to ...    27   7.5  
At2g40810.1 68415.m05034 WD-40 repeat protein family similar to ...    27   7.5  
At5g50280.1 68418.m06226 pentatricopeptide (PPR) repeat-containi...    27   9.9  

>At5g03650.1 68418.m00324 1,4-alpha-glucan branching enzyme / starch
           branching enzyme class II (SBE2-2) identical to starch
           branching enzyme class II [Arabidopsis thaliana]
           GI:726490
          Length = 716

 Score =  114 bits (274), Expect = 4e-26
 Identities = 55/126 (43%), Positives = 78/126 (61%), Gaps = 1/126 (0%)
 Frame = +1

Query: 112 PMEVPVP-DLEKLLERDGYLKPYEREIRRRYACYKDIWDRIESWDGGLEAFTKGYKYYGP 288
           P  VP P D +K+ E D  L+ Y   +  RY  YK + + I+ ++GGLEAF++GY+  G 
Sbjct: 24  PRIVPPPGDGKKIYEIDPMLRTYNNHLDYRYGQYKRLREEIDKYEGGLEAFSRGYEKLGF 83

Query: 289 QFNQDGSVTWREWAPGAHSLHLRGEFNGWDSKSHPFAKKEYGKWEIQIPANPDGSCALKH 468
               D  +T+REWAPGA +  L G+FN W+S +    + E+G WEI +P N DGS A+ H
Sbjct: 84  S-RSDAGITYREWAPGAKAASLIGDFNNWNSNADIMTRNEFGVWEIFLPNNTDGSPAIPH 142

Query: 469 DSRVQI 486
            SRV+I
Sbjct: 143 GSRVKI 148


>At2g36390.1 68415.m04466 1,4-alpha-glucan branching enzyme / starch
           branching enzyme class II (SBE2-1) nearly identical to
           starch branching enzyme class II [Arabidopsis thaliana]
           GI:619939
          Length = 858

 Score =  107 bits (257), Expect = 4e-24
 Identities = 50/124 (40%), Positives = 80/124 (64%), Gaps = 1/124 (0%)
 Frame = +1

Query: 118 EVPVP-DLEKLLERDGYLKPYEREIRRRYACYKDIWDRIESWDGGLEAFTKGYKYYGPQF 294
           ++P P D +++ + D  L  +   +  RY  Y+ + + I+  +GGLEAF++GY+ +G   
Sbjct: 150 KIPPPGDGKRIYDIDPMLNSHRNHLDYRYGQYRKLREEIDKNEGGLEAFSRGYEIFGFTR 209

Query: 295 NQDGSVTWREWAPGAHSLHLRGEFNGWDSKSHPFAKKEYGKWEIQIPANPDGSCALKHDS 474
           +  G +T+REWAPGA +  L G+FN W++KS   A+ ++G WEI +P N DGS A+ H S
Sbjct: 210 SATG-ITYREWAPGAKAASLIGDFNNWNAKSDVMARNDFGVWEIFLPNNADGSPAIPHGS 268

Query: 475 RVQI 486
           RV+I
Sbjct: 269 RVKI 272


>At3g20440.1 68416.m02588 glycoside hydrolase family 13 protein
           similar to 1,4-alpha-glucan branching enzyme [Solanum
           tuberosum] GI:1621012, 1,4-alpha-glucan branching enzyme
           (EC 2.4.1.18) from [Homo sapiens] SP|Q04446, {Solanum
           tuberosum} SP|P30924; contains Pfam profiles: PF00128
           Alpha amylase catalytic domain, PF02922 Isoamylase
           N-terminal domain
          Length = 777

 Score = 44.4 bits (100), Expect = 5e-05
 Identities = 23/85 (27%), Positives = 38/85 (44%), Gaps = 5/85 (5%)
 Frame = +1

Query: 187 IRRRYACYKDIWDRIESWDGGLEAFTKGYKYYGPQFNQDGSVTWREWAPGAHSLHLRGEF 366
           +R R+   KD+ D I         F  G++  G   + +  V + +W PG+    + G+F
Sbjct: 89  LRERHKALKDLKDEIFKRHFDFRDFASGFELLGMHRHMEHRVDFMDWGPGSRYGAIIGDF 148

Query: 367 NGWD-----SKSHPFAKKEYGKWEI 426
           NGW      ++   F   +YG W I
Sbjct: 149 NGWSPTENAAREGLFGHDDYGYWFI 173


>At3g06290.1 68416.m00722 SAC3/GANP family protein contains Pfam
           profile: PF03399 SAC3/GANP family
          Length = 1720

 Score = 31.1 bits (67), Expect = 0.46
 Identities = 14/38 (36%), Positives = 20/38 (52%)
 Frame = +3

Query: 165 FEAIRKRN*KEICLLQRHLGPYRIMGRWLRSIHEGLQI 278
           F   RK +  + CL+  H    R+  + L S+H GLQI
Sbjct: 714 FRLARKASYLQACLMHAHFSKVRLRTQALASLHSGLQI 751


>At2g21710.1 68415.m02582 mitochondrial transcription termination
           factor-related / mTERF-related contains Pfam profile
           PF02536: mTERF
          Length = 640

 Score = 29.5 bits (63), Expect = 1.4
 Identities = 16/45 (35%), Positives = 24/45 (53%)
 Frame = +3

Query: 129 TRFREIIRKRRLFEAIRKRN*KEICLLQRHLGPYRIMGRWLRSIH 263
           + F  ++R  +  E    R  K IC+ + +L   RIM  WL+SIH
Sbjct: 212 SNFVPLVRWLKHHELSYNRIAKIICMSKGNLDSIRIMIEWLKSIH 256


>At3g51770.1 68416.m05677 tetratricopeptide repeat (TPR)-containing
           protein contains Pfam profile PF00515: TPR Domain
          Length = 958

 Score = 28.3 bits (60), Expect = 3.2
 Identities = 17/45 (37%), Positives = 20/45 (44%)
 Frame = +3

Query: 39  LANCICKYIHNYLQYNGW*LFSYGSDGSACTRFREIIRKRRLFEA 173
           LA    K  H  L Y GW L+  G    A  +  E I  +R FEA
Sbjct: 696 LARNHSKSEHERLVYEGWILYDTGHREEALAKAEESISIQRSFEA 740


>At3g28960.1 68416.m03620 amino acid transporter family protein low
           similarity to vesicular inhibitory amino acid
           transporter [Mus musculus] GI:2826776; contains
           INTERPRO:IPR002422 amino acid/polyamine transporter,
           family II
          Length = 405

 Score = 27.5 bits (58), Expect = 5.7
 Identities = 13/30 (43%), Positives = 18/30 (60%)
 Frame = -2

Query: 473 ESCFNAHEPSGFAGI*ISHLPYSFLANGWL 384
           ++CFNA   +  +GI I  +PYS    GWL
Sbjct: 20  KTCFNAL--NALSGIGILSVPYSLARGGWL 47


>At2g27980.1 68415.m03391 expressed protein
          Length = 1037

 Score = 27.5 bits (58), Expect = 5.7
 Identities = 10/21 (47%), Positives = 14/21 (66%)
 Frame = -3

Query: 466 VSMHTNHLDLRGFESPICRTP 404
           V MH N +++ GF+SP   TP
Sbjct: 344 VDMHANDVEMDGFQSPSVTTP 364


>At1g25530.1 68414.m03170 lysine and histidine specific transporter,
           putative similar to lysine and histidine specific
           transporter GI:2576361 from [Arabidopsis thaliana];
           contains Pfam profile PF01490: Transmembrane amino acid
           transporter protein
          Length = 440

 Score = 27.5 bits (58), Expect = 5.7
 Identities = 25/125 (20%), Positives = 50/125 (40%), Gaps = 8/125 (6%)
 Frame = -1

Query: 513 YPVQVVVHY----DLDTGVVFQCTRTIWICGDLNLPFAVLLFSEWMALRVPSIEFTSKM* 346
           +PV ++ ++    D+D  V+    R  W+    NL   V +   +    +P  +   +M 
Sbjct: 274 FPVALICYWAFGQDVDDNVLMNLQRPAWLIAAANLMVVVHVIGSYQVFAMPVFDLLERM- 332

Query: 345 AMRSWSPLAPRNGAILIKLGSVVFVALRECF*ATVP*FDTVPNVFVA-SISPSNF---SF 178
                +    ++G +L      ++VA       + P F  +   F     +P++F   S 
Sbjct: 333 ---MVNKFGFKHGVVLRFFTRTIYVAFTLFIGVSFPFFGDLLGFFGGFGFAPTSFFLPSI 389

Query: 177 VWLQI 163
           +WL I
Sbjct: 390 MWLII 394


>At2g40810.2 68415.m05035 WD-40 repeat protein family similar to
           Gsa12p(GI:18307769)[Pichia pastoris]; contains 3 Pfam
           PF00400: WD domain, G-beta repeats
          Length = 393

 Score = 27.1 bits (57), Expect = 7.5
 Identities = 11/30 (36%), Positives = 15/30 (50%)
 Frame = +1

Query: 421 EIQIPANPDGSCALKHDSRVQIIVNDNLYR 510
           +I+  ANP G C L H S   ++    L R
Sbjct: 152 QIETQANPRGLCCLSHHSNTSVLACPGLNR 181


>At2g40810.1 68415.m05034 WD-40 repeat protein family similar to
           Gsa12p(GI:18307769)[Pichia pastoris]; contains 3 Pfam
           PF00400: WD domain, G-beta repeats
          Length = 393

 Score = 27.1 bits (57), Expect = 7.5
 Identities = 11/30 (36%), Positives = 15/30 (50%)
 Frame = +1

Query: 421 EIQIPANPDGSCALKHDSRVQIIVNDNLYR 510
           +I+  ANP G C L H S   ++    L R
Sbjct: 152 QIETQANPRGLCCLSHHSNTSVLACPGLNR 181


>At5g50280.1 68418.m06226 pentatricopeptide (PPR) repeat-containing
           protein contains Pfam profile PF01535: PPR repeat
          Length = 723

 Score = 26.6 bits (56), Expect = 9.9
 Identities = 10/32 (31%), Positives = 18/32 (56%)
 Frame = -1

Query: 462 QCTRTIWICGDLNLPFAVLLFSEWMALRVPSI 367
           +C   + + G+     + L F EWM+L+ PS+
Sbjct: 204 ECVEALVMMGESGFVKSCLYFYEWMSLQEPSL 235


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,642,752
Number of Sequences: 28952
Number of extensions: 288712
Number of successful extensions: 690
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 675
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 688
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 937669760
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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