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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= e96h0075
         (702 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g35020.1 68415.m04296 UTP--glucose-1-phosphate uridylyltransf...    76   2e-14
At1g31070.2 68414.m03804 UDP-N-acetylglucosamine pyrophosphoryla...    72   3e-13
At1g31070.1 68414.m03803 UDP-N-acetylglucosamine pyrophosphoryla...    47   1e-05
At2g47730.1 68415.m05960 glutathione S-transferase 6 (GST6) iden...    30   1.7  
At1g10770.1 68414.m01233 invertase/pectin methylesterase inhibit...    27   9.1  

>At2g35020.1 68415.m04296 UTP--glucose-1-phosphate
           uridylyltransferase family protein similar to SP|Q16222
           UDP-N-acetylhexosamine pyrophosphorylase (Antigen X)
           {Homo sapiens}; contains Pfam profile PF01704:
           UTP--glucose-1-phosphate uridylyltransferase
          Length = 502

 Score = 76.2 bits (179), Expect = 2e-14
 Identities = 45/120 (37%), Positives = 62/120 (51%), Gaps = 5/120 (4%)
 Frame = +3

Query: 357 SVPNLTPDKIEEYENIGFKEICNGKVGVLLLAGGQATRLGFGHPKGMYDVGCHQEKPCSR 536
           +V   T +  E++  +G K I  GK+GV+LL+GGQ TRLG   PKG Y++G    K   +
Sbjct: 100 TVEERTKEDREKWWKMGLKAIYEGKLGVVLLSGGQGTRLGSSDPKGCYNIGLPSGKSLFQ 159

Query: 537 YKPRGSWRVQQMAAEKYGNEG-----KITWYIMTSETHESPYCKIISKSHSYFGLNENDV 701
            +      VQ++A++            I WYIMTS     P  K   KSH YFGL  + V
Sbjct: 160 IQAERILCVQRLASQAMSEASPTRPVTIQWYIMTSPFTHEPTQKFF-KSHKYFGLEPDQV 218



 Score = 37.9 bits (84), Expect = 0.006
 Identities = 19/52 (36%), Positives = 29/52 (55%)
 Frame = +1

Query: 136 YETLLRNLKDHGQEHLIKYWSVLSEEQRKQLSDEILKLDLRKCMQHFRGQLN 291
           ++ L+  LKD+GQE +   W  LS E+R  L  +I  LDL +  +  R  L+
Sbjct: 30  HQALVERLKDYGQEDVFSLWDELSPEERDLLLRDIENLDLPRIDRIIRCSLH 81


>At1g31070.2 68414.m03804 UDP-N-acetylglucosamine
           pyrophosphorylase-related low similarity to SP|P43123
           UDP-N-acetylglucosamine pyrophosphorylase (EC 2.7.7.23)
           {Saccharomyces cerevisiae}
          Length = 505

 Score = 72.1 bits (169), Expect = 3e-13
 Identities = 45/120 (37%), Positives = 65/120 (54%), Gaps = 5/120 (4%)
 Frame = +3

Query: 357 SVPNLTPDKIEEYENIGFKEICNGKVGVLLLAGGQATRLGFGHPKGMYDVGCHQEKPCSR 536
           +V   T +  E++  +G K I  GK+GV+LL+GGQ TRLG   PKG +++G    K   +
Sbjct: 104 TVDGRTMEDREKWWKMGLKTIYEGKLGVVLLSGGQGTRLGSSDPKGCFNIGLPSGKSLFQ 163

Query: 537 YKPRGSWRVQQMAAEKYGNEGKIT-----WYIMTSETHESPYCKIISKSHSYFGLNENDV 701
            +      VQ++AA+   +EG I      WYIMTS   +    K  S SH YFGL  + +
Sbjct: 164 IQAERILCVQRLAAQVV-SEGPIRPVTIHWYIMTSPFTDEATRKYFS-SHKYFGLEPDQI 221



 Score = 34.7 bits (76), Expect = 0.060
 Identities = 17/51 (33%), Positives = 28/51 (54%)
 Frame = +1

Query: 139 ETLLRNLKDHGQEHLIKYWSVLSEEQRKQLSDEILKLDLRKCMQHFRGQLN 291
           + L+  LKD+GQE +   W  LS +++  L  +I  LDL +  +  R  L+
Sbjct: 35  QALVERLKDYGQEDIFSLWDELSPDEKDFLVRDIENLDLPRIDRIIRCSLH 85


>At1g31070.1 68414.m03803 UDP-N-acetylglucosamine
           pyrophosphorylase-related low similarity to SP|P43123
           UDP-N-acetylglucosamine pyrophosphorylase (EC 2.7.7.23)
           {Saccharomyces cerevisiae}
          Length = 153

 Score = 47.2 bits (107), Expect = 1e-05
 Identities = 22/48 (45%), Positives = 31/48 (64%)
 Frame = +3

Query: 357 SVPNLTPDKIEEYENIGFKEICNGKVGVLLLAGGQATRLGFGHPKGMY 500
           +V   T +  E++  +G K I  GK+GV+LL+GGQ TRLG   PKG +
Sbjct: 104 TVDGRTMEDREKWWKMGLKTIYEGKLGVVLLSGGQGTRLGSSDPKGCF 151



 Score = 34.7 bits (76), Expect = 0.060
 Identities = 17/51 (33%), Positives = 28/51 (54%)
 Frame = +1

Query: 139 ETLLRNLKDHGQEHLIKYWSVLSEEQRKQLSDEILKLDLRKCMQHFRGQLN 291
           + L+  LKD+GQE +   W  LS +++  L  +I  LDL +  +  R  L+
Sbjct: 35  QALVERLKDYGQEDIFSLWDELSPDEKDFLVRDIENLDLPRIDRIIRCSLH 85


>At2g47730.1 68415.m05960 glutathione S-transferase 6 (GST6)
           identical to GB:X95295. Based on identical cDNA hits,
           the translation is now 40 AAs longer at the N-terminal,
           and start of exon2 is also corrected.
          Length = 263

 Score = 29.9 bits (64), Expect = 1.7
 Identities = 17/66 (25%), Positives = 30/66 (45%)
 Frame = +1

Query: 61  HAF*SSYTVDEHLNLFVNKSRSEKMYETLLRNLKDHGQEHLIKYWSVLSEEQRKQLSDEI 240
           H F  S + + +  +  NKS S      ++ ++K HG         VL+    K L  E+
Sbjct: 20  HTFLHSSSSNSNFKIRSNKSSSSSSSSIIMASIKVHGVPMSTATMRVLATLYEKDLQFEL 79

Query: 241 LKLDLR 258
           + +D+R
Sbjct: 80  IPVDMR 85


>At1g10770.1 68414.m01233 invertase/pectin methylesterase inhibitor
           family protein contains Pfam profile PF04043: Plant
           invertase/pectin methylesterase inhibitor
          Length = 167

 Score = 27.5 bits (58), Expect = 9.1
 Identities = 21/82 (25%), Positives = 39/82 (47%)
 Frame = +3

Query: 42  AGQWASSRFLIVVYGRRAFKFVCKQIAE*KNV*NIITKPKRSWSRTLN*ILVGIK*GTAE 221
           AGQ A SR +I +    A+  +C+ + +       +T P+++  RT+  +    K   AE
Sbjct: 26  AGQVAESR-MINICSHTAYPSLCRPLVK------RVTSPRKATHRTIQALEAKTKLALAE 78

Query: 222 TAVGRNSKT*LTEVHATFSRAI 287
           TA  +N    ++  + T   A+
Sbjct: 79  TARFKNGNQAVSTCYETLGDAV 100


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,517,191
Number of Sequences: 28952
Number of extensions: 331197
Number of successful extensions: 851
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 809
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 846
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1506636208
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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