SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ceN-0071
         (416 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g24140.1 68414.m03045 matrixin family protein similar to matr...    77   6e-15
At1g59970.1 68414.m06755 matrixin family protein similar to SP|P...    76   8e-15
At2g45040.1 68415.m05607 matrix metalloproteinase nearly identic...    76   1e-14
At4g16640.1 68417.m02515 matrix metalloproteinase, putative meta...    69   1e-12
At1g70170.1 68414.m08074 matrixin family protein similar to SP|P...    65   2e-11
At3g01180.1 68416.m00023 glycogen synthase, putative similar to ...    31   0.41 
At1g77600.1 68414.m09035 expressed protein weak similarity to Pd...    29   1.3  
At5g35970.1 68418.m04332 DNA-binding protein, putative similar t...    28   2.9  
At5g52380.1 68418.m06499 zinc knuckle (CCHC-type) family protein...    27   3.8  
At5g12360.1 68418.m01454 expressed protein ; expression supporte...    27   5.1  
At5g45480.1 68418.m05587 expressed protein contains Pfam domain,...    27   6.7  
At1g58370.1 68414.m06640 glycosyl hydrolase family 10 protein / ...    27   6.7  

>At1g24140.1 68414.m03045 matrixin family protein similar to matrix
           metalloproteinase [Cucumis sativus] GI:7159629; contains
           InterPro accession IPR001818: Matrixin
          Length = 384

 Score = 76.6 bits (180), Expect = 6e-15
 Identities = 49/128 (38%), Positives = 60/128 (46%), Gaps = 10/128 (7%)
 Frame = +2

Query: 2   LVPLTFTQ-KRSGQVHIEIRFEKGEHXXXXXXXXXXXTLAHAYFPVYGGDAHFDDAEMWS 178
           + PLTFT+ +R     I I F  GEH           TLAHA+ P  G   H D  E W 
Sbjct: 203 VTPLTFTRVERFSTSDISIGFYSGEHGDGEPFDGPMRTLAHAFSPPTG-HFHLDGEENWI 261

Query: 179 INSRRG---------TNLFQVAAHEFGHSLGLSHSDVRSALMAPFYRGYDPAFQLDQDDV 331
           ++   G          +L  VA HE GH LGL HS V  ++M P  R       L  DDV
Sbjct: 262 VSGEGGDGFISVSEAVDLESVAVHEIGHLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDV 321

Query: 332 QGIQSLYG 355
           +G+Q LYG
Sbjct: 322 EGVQYLYG 329


>At1g59970.1 68414.m06755 matrixin family protein similar to
           SP|P29136 Metalloendoproteinase 1 precursor (EC
           3.4.24.-) (SMEP1) {Glycine max}; contains InterPro
           accession IPR001818: Matrixin
          Length = 360

 Score = 76.2 bits (179), Expect = 8e-15
 Identities = 54/135 (40%), Positives = 64/135 (47%), Gaps = 11/135 (8%)
 Frame = +2

Query: 2   LVPLTFTQKRSG-QVHIEIRFEKGEHXXXXXXXXXXXTLAHAYFPVYGGDAHFDDAEMWS 178
           + PL FT+  S  +  I I F  GEH           TLAHA  P  G   H D  E W 
Sbjct: 187 VTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAMGTLAHASSPPTGM-LHLDGDEDWL 245

Query: 179 IN----SRR------GTNLFQVAAHEFGHSLGLSHSDVRSALMAPFYRGYDPAFQLDQDD 328
           I+    SRR        +L  VA HE GH LGL HS V  A+M P   G D   +L +DD
Sbjct: 246 ISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSVEDAIMFPAISGGDRKVELAKDD 305

Query: 329 VQGIQSLYGHKTQTD 373
           ++GIQ LYG     D
Sbjct: 306 IEGIQHLYGGNPNGD 320


>At2g45040.1 68415.m05607 matrix metalloproteinase nearly identical
           to metalloproteinase [Arabidopsis thaliana] GI:3128477;
           contains InterPro accession IPR001818: Matrixin
          Length = 342

 Score = 75.8 bits (178), Expect = 1e-14
 Identities = 44/124 (35%), Positives = 61/124 (49%), Gaps = 6/124 (4%)
 Frame = +2

Query: 2   LVPLTFTQKRSGQV-HIEIRFEKGEHXXXXXXXXXXXTLAHAYFPVYGGDAHFDDAEMWS 178
           ++P++F +     +  I+I F  G+H            LAH + P   G  H D AE W+
Sbjct: 174 VIPVSFIETEDYVIADIKIGFFNGDHGDGEPFDGVLGVLAHTFSPE-NGRLHLDKAETWA 232

Query: 179 IN-----SRRGTNLFQVAAHEFGHSLGLSHSDVRSALMAPFYRGYDPAFQLDQDDVQGIQ 343
           ++     S    +L  VA HE GH LGL HS V+ A M P  +       L+ DDV G+Q
Sbjct: 233 VDFDEEKSSVAVDLESVAVHEIGHVLGLGHSSVKDAAMYPTLKPRSKKVNLNMDDVVGVQ 292

Query: 344 SLYG 355
           SLYG
Sbjct: 293 SLYG 296


>At4g16640.1 68417.m02515 matrix metalloproteinase, putative
           metalloproteinase [Arabidopsis thaliana] GI:3128477;
           contains InterPro accession IPR001818: Matrixin
          Length = 364

 Score = 69.3 bits (162), Expect = 1e-12
 Identities = 44/125 (35%), Positives = 58/125 (46%), Gaps = 7/125 (5%)
 Frame = +2

Query: 2   LVPLTFTQKRS-GQVHIEIRFEKGEHXXXXXXXXXXXTLAHAYFPVYGGDAHFDDAEMWS 178
           ++P++F +        ++I F  G+H           TLAHA+ P   G  H D AE W 
Sbjct: 196 VIPVSFEEVDDFTTADLKIGFYAGDHGDGLPFDGVLGTLAHAFAPE-NGRLHLDAAETWI 254

Query: 179 IN------SRRGTNLFQVAAHEFGHSLGLSHSDVRSALMAPFYRGYDPAFQLDQDDVQGI 340
           ++      S    +L  VA HE GH LGL HS   SA+M P  R       L  DDV G+
Sbjct: 255 VDDDLKGSSEVAVDLESVATHEIGHLLGLGHSSQESAVMYPSLRPRTKKVDLTVDDVAGV 314

Query: 341 QSLYG 355
             LYG
Sbjct: 315 LKLYG 319


>At1g70170.1 68414.m08074 matrixin family protein similar to
           SP|P29136 Metalloendoproteinase 1 precursor (EC
           3.4.24.-) (SMEP1) {Glycine max}; contains InterPro
           accession IPR001818: Matrixin
          Length = 378

 Score = 65.3 bits (152), Expect = 2e-11
 Identities = 45/124 (36%), Positives = 53/124 (42%), Gaps = 9/124 (7%)
 Frame = +2

Query: 11  LTFTQKRSGQVH-IEIRFEKGEHXXXXXXXXXXXTLAHAYFPVYGGDAHFDDAEMWSINS 187
           L FT   S     I I F  G+H           TLAHA+ P   G  H D  E W ++ 
Sbjct: 202 LNFTLSESFSTSDITIGFYTGDHGDGEPFDGVLGTLAHAFSPP-SGKFHLDADENWVVSG 260

Query: 188 --------RRGTNLFQVAAHEFGHSLGLSHSDVRSALMAPFYRGYDPAFQLDQDDVQGIQ 343
                       +L  VA HE GH LGL HS V  ++M P          L  DDV+GIQ
Sbjct: 261 DLDSFLSVTAAVDLESVAVHEIGHLLGLGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQ 320

Query: 344 SLYG 355
            LYG
Sbjct: 321 YLYG 324


>At3g01180.1 68416.m00023 glycogen synthase, putative similar to
           glycogen synthase Q43847 from [Solanum tuberosum]
          Length = 792

 Score = 30.7 bits (66), Expect = 0.41
 Identities = 11/43 (25%), Positives = 21/43 (48%)
 Frame = -2

Query: 214 YLEKISSSTGIDGPHLSVIKMGITSIHREISVGEGASWTIKRI 86
           YL+       + G H ++   G+ +  R ++V  G SW +K +
Sbjct: 491 YLDSFKLYDPVGGEHFNIFAAGLKAADRVLTVSHGYSWEVKTL 533


>At1g77600.1 68414.m09035 expressed protein weak similarity to Pds5
           (GI:16751524) [Schizosaccharomyces pombe]; weak
           similarity to androgen-induced prostate proliferative
           shutoff associated protein (GI:4559410) [Homo sapiens]
          Length = 1285

 Score = 29.1 bits (62), Expect = 1.3
 Identities = 12/25 (48%), Positives = 17/25 (68%)
 Frame = +1

Query: 235 LAGSLTQRCKIRIDGAVLSWLRSCF 309
           LA SL +RC  R++  + S+L SCF
Sbjct: 226 LASSLIERCADRLEPLICSFLTSCF 250


>At5g35970.1 68418.m04332 DNA-binding protein, putative similar to
           SWISS-PROT:Q60560 DNA-binding protein SMUBP-2
           (Immunoglobulin MU binding protein 2, SMUBP-2)
           [Mesocricetus auratus]
          Length = 961

 Score = 27.9 bits (59), Expect = 2.9
 Identities = 11/23 (47%), Positives = 16/23 (69%)
 Frame = -2

Query: 217 CYLEKISSSTGIDGPHLSVIKMG 149
           C L  +S+STG+ G HL + K+G
Sbjct: 324 CNLYAVSTSTGLGGMHLVLFKVG 346


>At5g52380.1 68418.m06499 zinc knuckle (CCHC-type) family protein
           contains Pfam domain, PF00098: Zinc knuckle
          Length = 268

 Score = 27.5 bits (58), Expect = 3.8
 Identities = 13/28 (46%), Positives = 15/28 (53%)
 Frame = -1

Query: 149 HHLHTPGNKRGRGCLLDHQKDLRHRARL 66
           H L  PG K G GC + H K   H A+L
Sbjct: 63  HPLRVPGMKPGEGCFICHSK--THIAKL 88


>At5g12360.1 68418.m01454 expressed protein ; expression supported
           by MPSS
          Length = 194

 Score = 27.1 bits (57), Expect = 5.1
 Identities = 10/27 (37%), Positives = 16/27 (59%), Gaps = 2/27 (7%)
 Frame = -3

Query: 213 TWKRL--VPLRELMDHISASSKWASPP 139
           +W  L  +P+ E  D +  S++W SPP
Sbjct: 135 SWDELKEIPMEEFSDLLECSTQWRSPP 161


>At5g45480.1 68418.m05587 expressed protein contains Pfam domain,
           PF04578: Protein of unknown function, DUF594
          Length = 877

 Score = 26.6 bits (56), Expect = 6.7
 Identities = 24/66 (36%), Positives = 33/66 (50%), Gaps = 7/66 (10%)
 Frame = +2

Query: 158 DDAEMWSINSRRGTNLFQVAAHEFG---HSLGLSH-SDVRS---ALMAPFYRGYDPAFQL 316
           DD+EMW I S+    L   AA + G   H+  LS   ++ S    LMA F  G    FQ+
Sbjct: 807 DDSEMWKIMSQVWVELLSYAATKCGAIEHAAQLSKGGELISFVWLLMAHF--GLGDQFQI 864

Query: 317 DQDDVQ 334
           +Q D +
Sbjct: 865 NQGDAR 870


>At1g58370.1 68414.m06640 glycosyl hydrolase family 10 protein /
           carbohydrate-binding domain-containing protein similar
           to (1,4)-beta-xylan endohydrolase GI:5306060 from
           [Triticum aestivum] ; contains Pfam profiles PF00331:
           Glycosyl hydrolase family 10, PF02018: Carbohydrate
           binding domain
          Length = 917

 Score = 26.6 bits (56), Expect = 6.7
 Identities = 19/75 (25%), Positives = 37/75 (49%), Gaps = 9/75 (12%)
 Frame = -2

Query: 340 NSLDVVLIELESRIVTTIKRRHQCGSYIAV---*ET-----QRVTKLMR-CYLEKISSST 188
           N  +  ++  ES +   +    +CGSY+ V    ET     Q +T  ++ C L K+S++ 
Sbjct: 46  NCCEAFVVTAESNVSHGVLDPSKCGSYVVVKNRKETWQGLEQDITNRVKPCSLYKVSATV 105

Query: 187 GIDGPHLSVIKMGIT 143
            + GP   ++++  T
Sbjct: 106 AVSGPVHGLVEVMAT 120


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,343,007
Number of Sequences: 28952
Number of extensions: 187030
Number of successful extensions: 511
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 492
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 504
length of database: 12,070,560
effective HSP length: 74
effective length of database: 9,928,112
effective search space used: 635399168
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -