Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ce--0061
(516 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g58420.1 68418.m07315 40S ribosomal protein S4 (RPS4D) riboso... 162 1e-40
At5g07090.1 68418.m00804 40S ribosomal protein S4 (RPS4B) 162 1e-40
At2g17360.1 68415.m02005 40S ribosomal protein S4 (RPS4A) contai... 162 1e-40
At4g10710.1 68417.m01751 transcriptional regulator-related simil... 31 0.46
At1g29760.1 68414.m03639 expressed protein 31 0.46
At4g08180.3 68417.m01353 oxysterol-binding family protein simila... 29 1.9
At4g08180.2 68417.m01352 oxysterol-binding family protein simila... 29 1.9
At4g08180.1 68417.m01351 oxysterol-binding family protein simila... 29 1.9
At2g38050.1 68415.m04671 3-oxo-5-alpha-steroid 4-dehydrogenase, ... 29 1.9
At1g24150.1 68414.m03047 formin homology 2 domain-containing pro... 27 5.7
At5g67100.1 68418.m08460 DNA-directed DNA polymerase alpha catal... 27 9.9
At5g03980.1 68418.m00378 GDSL-motif lipase/hydrolase family prot... 27 9.9
At4g38440.1 68417.m05432 expressed protein 27 9.9
At4g13190.1 68417.m02051 protein kinase family protein similar t... 27 9.9
At1g50260.1 68414.m05635 C2 domain-containing protein low simila... 27 9.9
>At5g58420.1 68418.m07315 40S ribosomal protein S4 (RPS4D) ribosomal
protein S4, Arabidopsis thaliana, PIR:T48480
Length = 262
Score = 162 bits (393), Expect = 1e-40
Identities = 81/144 (56%), Positives = 99/144 (68%), Gaps = 2/144 (1%)
Frame = +2
Query: 2 RLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNEVLKIVKQRLI 181
RLNAPK WMLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT EV+ I+ QR I
Sbjct: 11 RLNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYREVISILMQRHI 70
Query: 182 KVDGKVRTDPTYPAGFMDVVSIEKIMNCFV*SMMLRADSQSTVSPLR--RLSTKLCKVKR 355
+VDGKVRTD TYPAGFMDVVSI K F ++ + + ++ KLCKV+
Sbjct: 71 QVDGKVRTDKTYPAGFMDVVSIPKTNENF--RLLYDTKGRFRLHSIKDEEAKFKLCKVRS 128
Query: 356 VATGPKNVPYLVTHDGPYHPLPRP 427
+ G K +PYL T+DG P P
Sbjct: 129 IQFGQKGIPYLNTYDGRTIRYPDP 152
Score = 46.8 bits (106), Expect = 9e-06
Identities = 21/37 (56%), Positives = 26/37 (70%)
Frame = +3
Query: 405 RTIRYPDPLIKSQRFHPVDIATTKIMDFIKFESGNFV 515
RTIRYPDPLIK +D+ KI++FIKF+ GN V
Sbjct: 145 RTIRYPDPLIKPNDTIKLDLEANKIVEFIKFDVGNVV 181
>At5g07090.1 68418.m00804 40S ribosomal protein S4 (RPS4B)
Length = 262
Score = 162 bits (393), Expect = 1e-40
Identities = 81/144 (56%), Positives = 99/144 (68%), Gaps = 2/144 (1%)
Frame = +2
Query: 2 RLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNEVLKIVKQRLI 181
RLNAPK WMLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT EV+ I+ QR I
Sbjct: 11 RLNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYREVISILMQRHI 70
Query: 182 KVDGKVRTDPTYPAGFMDVVSIEKIMNCFV*SMMLRADSQSTVSPLR--RLSTKLCKVKR 355
+VDGKVRTD TYPAGFMDVVSI K F ++ + + ++ KLCKV+
Sbjct: 71 QVDGKVRTDKTYPAGFMDVVSIPKTNENF--RLLYDTKGRFRLHSIKDEEAKFKLCKVRS 128
Query: 356 VATGPKNVPYLVTHDGPYHPLPRP 427
+ G K +PYL T+DG P P
Sbjct: 129 IQFGQKGIPYLNTYDGRTIRYPDP 152
Score = 46.4 bits (105), Expect = 1e-05
Identities = 21/37 (56%), Positives = 26/37 (70%)
Frame = +3
Query: 405 RTIRYPDPLIKSQRFHPVDIATTKIMDFIKFESGNFV 515
RTIRYPDPLIK +D+ KI++FIKF+ GN V
Sbjct: 145 RTIRYPDPLIKPNDTIKLDLEENKIVEFIKFDVGNVV 181
>At2g17360.1 68415.m02005 40S ribosomal protein S4 (RPS4A) contains
ribosomal protein S4 signature from residues 8 to 22
Length = 261
Score = 162 bits (393), Expect = 1e-40
Identities = 81/144 (56%), Positives = 99/144 (68%), Gaps = 2/144 (1%)
Frame = +2
Query: 2 RLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNEVLKIVKQRLI 181
RLNAPK WMLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT EV+ I+ QR I
Sbjct: 11 RLNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYREVISILMQRHI 70
Query: 182 KVDGKVRTDPTYPAGFMDVVSIEKIMNCFV*SMMLRADSQSTVSPLR--RLSTKLCKVKR 355
+VDGKVRTD TYPAGFMDVVSI K F ++ + + ++ KLCKV+
Sbjct: 71 QVDGKVRTDKTYPAGFMDVVSIPKTNENF--RLLYDTKGRFRLHSIKDEEAKFKLCKVRS 128
Query: 356 VATGPKNVPYLVTHDGPYHPLPRP 427
+ G K +PYL T+DG P P
Sbjct: 129 IQFGQKGIPYLNTYDGRTIRYPDP 152
Score = 46.4 bits (105), Expect = 1e-05
Identities = 21/37 (56%), Positives = 26/37 (70%)
Frame = +3
Query: 405 RTIRYPDPLIKSQRFHPVDIATTKIMDFIKFESGNFV 515
RTIRYPDPLIK +D+ KI++FIKF+ GN V
Sbjct: 145 RTIRYPDPLIKPNDTIKLDLEENKIVEFIKFDVGNVV 181
>At4g10710.1 68417.m01751 transcriptional regulator-related similar
to chromatin-specific transcription elongation factor
FACT 140 kDa subunit (GI:5499741) [Homo sapiens]
Length = 1074
Score = 31.1 bits (67), Expect = 0.46
Identities = 12/27 (44%), Positives = 16/27 (59%)
Frame = -1
Query: 96 KHSRNLWGPVDGLGAYTPPSLSNIHAL 16
KHS +LWG D L TPP+ ++ L
Sbjct: 44 KHSADLWGSADALAIATPPASDDLRYL 70
>At1g29760.1 68414.m03639 expressed protein
Length = 526
Score = 31.1 bits (67), Expect = 0.46
Identities = 14/31 (45%), Positives = 17/31 (54%)
Frame = -1
Query: 207 SVLTFPSTFMRRCFTIFRTSFPVKAYFRRFL 115
S+LTFP +R CF F F + RRFL
Sbjct: 195 SLLTFPPWLLRNCFLFFFDPFSTIRFGRRFL 225
>At4g08180.3 68417.m01353 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 813
Score = 29.1 bits (62), Expect = 1.9
Identities = 15/47 (31%), Positives = 27/47 (57%), Gaps = 1/47 (2%)
Frame = +2
Query: 119 NRLKYALTGNEVLKIVKQRLIKVDGKVRTDPTY-PAGFMDVVSIEKI 256
N ++A+T NE+ +K+RL D ++R D Y G ++ + EK+
Sbjct: 703 NLTRFAITLNELTPGLKERLPPTDSRLRPDQRYLENGEFEMANTEKL 749
>At4g08180.2 68417.m01352 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 813
Score = 29.1 bits (62), Expect = 1.9
Identities = 15/47 (31%), Positives = 27/47 (57%), Gaps = 1/47 (2%)
Frame = +2
Query: 119 NRLKYALTGNEVLKIVKQRLIKVDGKVRTDPTY-PAGFMDVVSIEKI 256
N ++A+T NE+ +K+RL D ++R D Y G ++ + EK+
Sbjct: 703 NLTRFAITLNELTPGLKERLPPTDSRLRPDQRYLENGEFEMANTEKL 749
>At4g08180.1 68417.m01351 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 814
Score = 29.1 bits (62), Expect = 1.9
Identities = 15/47 (31%), Positives = 27/47 (57%), Gaps = 1/47 (2%)
Frame = +2
Query: 119 NRLKYALTGNEVLKIVKQRLIKVDGKVRTDPTY-PAGFMDVVSIEKI 256
N ++A+T NE+ +K+RL D ++R D Y G ++ + EK+
Sbjct: 704 NLTRFAITLNELTPGLKERLPPTDSRLRPDQRYLENGEFEMANTEKL 750
>At2g38050.1 68415.m04671 3-oxo-5-alpha-steroid 4-dehydrogenase,
putative / steroid 5-alpha-reductase, putative identical
to gi:1280611; contains a steroid 5-alpha reductase,
C-terminal domain
Length = 262
Score = 29.1 bits (62), Expect = 1.9
Identities = 15/39 (38%), Positives = 19/39 (48%)
Frame = -1
Query: 186 TFMRRCFTIFRTSFPVKAYFRRFLRKITRGKHSRNLWGP 70
TF R C + P A +FL+ GKH+R WGP
Sbjct: 8 TFFRYCLLTLIFAGPPTAVLLKFLQA-PYGKHNRTGWGP 45
>At1g24150.1 68414.m03047 formin homology 2 domain-containing
protein / FH2 domain-containing protein contains formin
homology 2 domain, Pfam:PF02181
Length = 725
Score = 27.5 bits (58), Expect = 5.7
Identities = 14/37 (37%), Positives = 21/37 (56%)
Frame = +2
Query: 347 VKRVATGPKNVPYLVTHDGPYHPLPRPTYQKSTISSS 457
VK+ AT P P + ++GP P P P + + +SSS
Sbjct: 253 VKQSATPPPPPPPKLKNNGPSPPPPPPLKKTAALSSS 289
>At5g67100.1 68418.m08460 DNA-directed DNA polymerase alpha
catalytic subunit, putative similar to SP|O48653 DNA
polymerase alpha catalytic subunit (EC 2.7.7.7) {Oryza
sativa}; contains Pfam profiles: PF03175 DNA polymerase
type B, organellar and viral, PF00136 DNA polymerase
family B, PF03104 DNA polymerase family B, exonuclease
domain
Length = 1492
Score = 26.6 bits (56), Expect = 9.9
Identities = 15/44 (34%), Positives = 22/44 (50%)
Frame = +2
Query: 284 LRADSQSTVSPLRRLSTKLCKVKRVATGPKNVPYLVTHDGPYHP 415
L+ S ST SP +S CK + PK++ LV+ + HP
Sbjct: 518 LKISSFSTCSPSEGVSW--CKFEVTVQSPKDITILVSEEKVVHP 559
>At5g03980.1 68418.m00378 GDSL-motif lipase/hydrolase family protein
similar to lipase [Arabidopsis thaliana] GI:1145627;
contains Pfam profile:PF00657 Lipase/Acylhydrolase with
GDSL-like motif
Length = 323
Score = 26.6 bits (56), Expect = 9.9
Identities = 11/19 (57%), Positives = 12/19 (63%)
Frame = -1
Query: 192 PSTFMRRCFTIFRTSFPVK 136
P F CF I+ TSFPVK
Sbjct: 175 PGNFPVGCFPIYLTSFPVK 193
>At4g38440.1 68417.m05432 expressed protein
Length = 1465
Score = 26.6 bits (56), Expect = 9.9
Identities = 11/32 (34%), Positives = 17/32 (53%)
Frame = +2
Query: 2 RLNAPKAWMLDKLGGVYAPRPSTGPHKLRECL 97
R+ P W L + V++ + STGP + E L
Sbjct: 1141 RMPLPPHWFLSAISAVHSGKTSTGPPESTELL 1172
>At4g13190.1 68417.m02051 protein kinase family protein similar to
serine/threonine kinase BNK1 [Brassica napus]
gi|10445209|gb|AAG16628; contains protein kinase domain,
Pfam:PF00069
Length = 405
Score = 26.6 bits (56), Expect = 9.9
Identities = 12/29 (41%), Positives = 16/29 (55%)
Frame = +1
Query: 37 TWRCVCTETVNRSPQVARVLAPGDFPEES 123
TW NR P++A L G+FPE+S
Sbjct: 304 TWAQPIFREPNRFPELADPLLQGEFPEKS 332
>At1g50260.1 68414.m05635 C2 domain-containing protein low
similarity to CLB1 [Lycopersicon esculentum] GI:2789434;
contains Pfam profile PF00168: C2 domain
Length = 675
Score = 26.6 bits (56), Expect = 9.9
Identities = 13/25 (52%), Positives = 14/25 (56%)
Frame = +2
Query: 377 VPYLVTHDGPYHPLPRPTYQKSTIS 451
V L T G HPLP PT +S IS
Sbjct: 110 VDKLRTQLGVIHPLPSPTINRSVIS 134
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,053,669
Number of Sequences: 28952
Number of extensions: 254862
Number of successful extensions: 699
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 680
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 693
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 937669760
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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