SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-0064
         (377 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g48970.1 68414.m05489 eukaryotic translation initiation facto...    31   0.34 
At2g44070.1 68415.m05481 eukaryotic translation initiation facto...    28   1.8  
At4g38560.1 68417.m05459 expressed protein                             28   2.4  
At5g38640.1 68418.m04673 eukaryotic translation initiation facto...    27   4.1  
At5g48670.1 68418.m06023 MADS-box family protein contains simila...    27   5.5  
At3g26350.1 68416.m03287 expressed protein  ; expression support...    26   7.2  
At5g25590.1 68418.m03045 expressed protein contains Pfam profile...    26   9.6  
At4g05440.1 68417.m00826 temperature sensing protein-related con...    26   9.6  

>At1g48970.1 68414.m05489 eukaryotic translation initiation factor
           2B family protein / eIF-2B family protein similar to
           guanine nucleotide exchange factor, eIF-2B, delta
           subunit [Mus musculus] GI:529428; contains Pfam profile
           PF01008: Initiation factor 2 subunit family
          Length = 756

 Score = 30.7 bits (66), Expect = 0.34
 Identities = 20/64 (31%), Positives = 30/64 (46%), Gaps = 2/64 (3%)
 Frame = +2

Query: 188 SITSQAREHVRADEVLLTFGASALVERFL--RPTXNRNCKVVVAEGTDVGESHAMARRLS 361
           +I   A   +R  EVLLT+G S +VE  L       +  +VV+ +     E   + RRL 
Sbjct: 540 AIVQHAVTKIRDGEVLLTYGFSCVVEMILLYAHEIGKKFRVVIVDSRPNLEGQKLLRRLV 599

Query: 362 NSGV 373
             G+
Sbjct: 600 TRGL 603


>At2g44070.1 68415.m05481 eukaryotic translation initiation factor
           2B family protein / eIF-2B family protein similar to
           SP|Q63186 Translation initiation factor eIF-2B delta
           subunit (eIF-2B GDP-GTP exchange factor) {Rattus
           norvegicus}; contains Pfam profile PF01008: Initiation
           factor 2 subunit family
          Length = 307

 Score = 28.3 bits (60), Expect = 1.8
 Identities = 17/64 (26%), Positives = 30/64 (46%), Gaps = 2/64 (3%)
 Frame = +2

Query: 191 ITSQAREHVRADEVLLTFGASALVERFL--RPTXNRNCKVVVAEGTDVGESHAMARRLSN 364
           I   A   +R  +VLLT+G+S ++E  L       +  +V V +     +   + RRL  
Sbjct: 91  IVEHAVTKIRDGDVLLTYGSSTVIEMILVHAHELGKKFRVFVVDSRPKLQGKLLLRRLIK 150

Query: 365 SGVS 376
            G++
Sbjct: 151 RGIN 154


>At4g38560.1 68417.m05459 expressed protein
          Length = 521

 Score = 27.9 bits (59), Expect = 2.4
 Identities = 16/47 (34%), Positives = 20/47 (42%)
 Frame = +1

Query: 13  QSFRRPVPEW*GCRRESPRSGSGDSGPAQHRAFNGSGLEGTNKRSHR 153
           Q  R   PE  G   E PRS    + P  HR+   +GL+   K   R
Sbjct: 172 QGPRSKTPEPRGSYLEPPRSRIPQTQPVPHRSLESAGLKSPQKGETR 218


>At5g38640.1 68418.m04673 eukaryotic translation initiation factor
           2B family protein / eIF-2B family protein similar to
           SP|P41111 Translation initiation factor eIF-2B delta
           subunit (eIF-2B GDP-GTP exchange factor) {Oryctolagus
           cuniculus}; contains Pfam profile PF01008: Initiation
           factor 2 subunit family
          Length = 642

 Score = 27.1 bits (57), Expect = 4.1
 Identities = 17/64 (26%), Positives = 29/64 (45%), Gaps = 2/64 (3%)
 Frame = +2

Query: 191 ITSQAREHVRADEVLLTFGASALVERFL--RPTXNRNCKVVVAEGTDVGESHAMARRLSN 364
           I   A   +R  +VLLT+G+   VE  +       +  +V+V +     E   + RRL  
Sbjct: 433 IVKHAVTKIRDGDVLLTYGSPTAVEMVILHAHELRKKFRVLVVDSRPKLEGQLLLRRLIK 492

Query: 365 SGVS 376
            G++
Sbjct: 493 RGIN 496


>At5g48670.1 68418.m06023 MADS-box family protein contains
           similarity to MADS-box DNA-binding protein
          Length = 321

 Score = 26.6 bits (56), Expect = 5.5
 Identities = 11/39 (28%), Positives = 24/39 (61%)
 Frame = +2

Query: 257 LVERFLRPTXNRNCKVVVAEGTDVGESHAMARRLSNSGV 373
           ++E++L+   NR  +++   GT++GES ++A   S   +
Sbjct: 141 MIEQYLKDV-NRRIEILRNSGTEIGESSSVAVAASEGNI 178


>At3g26350.1 68416.m03287 expressed protein  ; expression supported
           by MPSS
          Length = 356

 Score = 26.2 bits (55), Expect = 7.2
 Identities = 8/15 (53%), Positives = 11/15 (73%)
 Frame = -3

Query: 84  VARTRPWRLSPTPSP 40
           + +  PWR +PTPSP
Sbjct: 140 IPKPSPWRTAPTPSP 154


>At5g25590.1 68418.m03045 expressed protein contains Pfam profile
           PF04783: Protein of unknown function (DUF630)
          Length = 775

 Score = 25.8 bits (54), Expect = 9.6
 Identities = 9/27 (33%), Positives = 15/27 (55%)
 Frame = +2

Query: 140 RDHIAELRTELETMRSSITSQAREHVR 220
           RDH+ E R  +ET++  +  +   H R
Sbjct: 694 RDHVTERRIAVETLKKRLEEEEEAHQR 720


>At4g05440.1 68417.m00826 temperature sensing protein-related
           contains weak similarity to D123 (GI:1236114) [Rattus
           norvegicus]
          Length = 333

 Score = 25.8 bits (54), Expect = 9.6
 Identities = 16/43 (37%), Positives = 20/43 (46%), Gaps = 6/43 (13%)
 Frame = -2

Query: 346 HGVTLADVSPFR------DDDLAVPIXGRPQEPLDQSAGPERE 236
           H VT  D  P R      +DD  VP      EPL++ + PE E
Sbjct: 49  HSVTNEDAMPNRVHNFEEEDDFQVPEEASDDEPLNRPSFPELE 91


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 7,666,092
Number of Sequences: 28952
Number of extensions: 138669
Number of successful extensions: 419
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 414
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 419
length of database: 12,070,560
effective HSP length: 73
effective length of database: 9,957,064
effective search space used: 517767328
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -