Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--0227
(654 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g04430.2 68418.m00438 KH domain-containing protein NOVA, puta... 36 0.031
At5g04430.1 68418.m00437 KH domain-containing protein NOVA, puta... 36 0.031
At5g64390.2 68418.m08088 KH domain-containing RNA-binding protei... 34 0.095
At5g64390.1 68418.m08089 KH domain-containing RNA-binding protei... 34 0.095
At2g25970.1 68415.m03117 KH domain-containing protein 34 0.095
At2g22600.1 68415.m02679 KH domain-containing protein 33 0.13
At3g08620.1 68416.m01001 KH domain-containing protein 32 0.29
At1g14170.1 68414.m01675 KH domain-containing protein location o... 32 0.29
At5g09560.1 68418.m01107 KH domain-containing protein various pr... 32 0.38
At5g06770.1 68418.m00765 KH domain-containing protein / zinc fin... 32 0.38
At5g46190.1 68418.m05681 KH domain-containing protein strong sim... 31 0.51
At1g33680.1 68414.m04166 KH domain-containing protein similar to... 31 0.67
At4g18375.2 68417.m02727 KH domain-containing protein contains s... 30 1.5
At4g18375.1 68417.m02726 KH domain-containing protein contains s... 30 1.5
At1g51580.1 68414.m05806 KH domain-containing protein 30 1.5
At3g12130.1 68416.m01509 KH domain-containing protein / zinc fin... 29 2.0
At5g51300.2 68418.m06360 splicing factor-related contains simila... 28 4.7
At5g51300.1 68418.m06359 splicing factor-related contains simila... 28 4.7
At5g56370.2 68418.m07037 F-box family protein contains F-box dom... 28 6.2
At5g56370.1 68418.m07036 F-box family protein contains F-box dom... 28 6.2
At5g23430.2 68418.m02749 transducin family protein / WD-40 repea... 28 6.2
At5g23430.1 68418.m02748 transducin family protein / WD-40 repea... 28 6.2
At2g34670.1 68415.m04259 proline-rich family protein contains pr... 28 6.2
>At5g04430.2 68418.m00438 KH domain-containing protein NOVA,
putative astrocytic NOVA-like RNA-binding protein, Homo
sapiens, U70477
Length = 334
Score = 35.5 bits (78), Expect = 0.031
Identities = 15/38 (39%), Positives = 26/38 (68%)
Frame = +2
Query: 110 QFVLSQNLVGLLIGKHGSFVTQIKAKTGASVYVRRHPE 223
+F++S G +IGK GS +T+ +AK+GA + + R+ E
Sbjct: 38 RFLVSNAAAGSVIGKGGSTITEFQAKSGARIQLSRNQE 75
>At5g04430.1 68418.m00437 KH domain-containing protein NOVA,
putative astrocytic NOVA-like RNA-binding protein, Homo
sapiens, U70477
Length = 313
Score = 35.5 bits (78), Expect = 0.031
Identities = 15/38 (39%), Positives = 26/38 (68%)
Frame = +2
Query: 110 QFVLSQNLVGLLIGKHGSFVTQIKAKTGASVYVRRHPE 223
+F++S G +IGK GS +T+ +AK+GA + + R+ E
Sbjct: 38 RFLVSNAAAGSVIGKGGSTITEFQAKSGARIQLSRNQE 75
>At5g64390.2 68418.m08088 KH domain-containing RNA-binding protein
(HEN4) contains similarity to RNA-binding protein;
identical to cDNA HEN4 isoform 2 (HEN4) GI:28261404;
contains Pfam domain PF00013: KH domain; identical to
cDNA HEN4 (HEN4) GI:28261402
Length = 824
Score = 33.9 bits (74), Expect = 0.095
Identities = 13/39 (33%), Positives = 22/39 (56%)
Frame = +2
Query: 134 VGLLIGKHGSFVTQIKAKTGASVYVRRHPESSKQKICAV 250
VG +IGK G+ + Q++ TGA + V P S ++ +
Sbjct: 58 VGAVIGKSGNVIKQLQQSTGAKIRVEEPPSGSPDRVITI 96
Score = 27.9 bits (59), Expect = 6.2
Identities = 10/41 (24%), Positives = 26/41 (63%)
Frame = +2
Query: 110 QFVLSQNLVGLLIGKHGSFVTQIKAKTGASVYVRRHPESSK 232
+ V+ + +G ++GK G V++++ TGA++ + + ++ K
Sbjct: 545 RLVVPTSQIGCVLGKGGVIVSEMRKTTGAAIQILKVEQNPK 585
>At5g64390.1 68418.m08089 KH domain-containing RNA-binding protein
(HEN4) contains similarity to RNA-binding protein;
identical to cDNA HEN4 isoform 2 (HEN4) GI:28261404;
contains Pfam domain PF00013: KH domain; identical to
cDNA HEN4 (HEN4) GI:28261402
Length = 857
Score = 33.9 bits (74), Expect = 0.095
Identities = 13/39 (33%), Positives = 22/39 (56%)
Frame = +2
Query: 134 VGLLIGKHGSFVTQIKAKTGASVYVRRHPESSKQKICAV 250
VG +IGK G+ + Q++ TGA + V P S ++ +
Sbjct: 58 VGAVIGKSGNVIKQLQQSTGAKIRVEEPPSGSPDRVITI 96
Score = 27.9 bits (59), Expect = 6.2
Identities = 10/41 (24%), Positives = 26/41 (63%)
Frame = +2
Query: 110 QFVLSQNLVGLLIGKHGSFVTQIKAKTGASVYVRRHPESSK 232
+ V+ + +G ++GK G V++++ TGA++ + + ++ K
Sbjct: 545 RLVVPTSQIGCVLGKGGVIVSEMRKTTGAAIQILKVEQNPK 585
>At2g25970.1 68415.m03117 KH domain-containing protein
Length = 632
Score = 33.9 bits (74), Expect = 0.095
Identities = 17/35 (48%), Positives = 23/35 (65%), Gaps = 2/35 (5%)
Frame = +2
Query: 110 QFVLS--QNLVGLLIGKHGSFVTQIKAKTGASVYV 208
QFV+ N VGL+IGK G + ++AKTGA + V
Sbjct: 231 QFVMKIPNNKVGLIIGKGGETIKSMQAKTGARIQV 265
>At2g22600.1 68415.m02679 KH domain-containing protein
Length = 632
Score = 33.5 bits (73), Expect = 0.13
Identities = 15/58 (25%), Positives = 35/58 (60%)
Frame = +2
Query: 32 KGCSEAASPPPASLNIVPTETDLRIHQFVLSQNLVGLLIGKHGSFVTQIKAKTGASVY 205
+G + A S ++++P ET +R+ V +++G +IG +G V++++ +TG ++
Sbjct: 5 RGVTTAVSKRRPVIHVLPDETAIRV---VCHASVIGGIIGSNGYVVSKLRRETGTKIH 59
Score = 28.7 bits (61), Expect = 3.6
Identities = 12/49 (24%), Positives = 28/49 (57%)
Frame = +2
Query: 104 IHQFVLSQNLVGLLIGKHGSFVTQIKAKTGASVYVRRHPESSKQKICAV 250
+ + + + + G +IGK G+ + ++ +TGAS+ V + S +++ V
Sbjct: 278 VFKIIFTSVVAGGIIGKQGTIIRALQNETGASISVGAPLKVSGERVVTV 326
>At3g08620.1 68416.m01001 KH domain-containing protein
Length = 283
Score = 32.3 bits (70), Expect = 0.29
Identities = 19/53 (35%), Positives = 30/53 (56%), Gaps = 3/53 (5%)
Frame = +2
Query: 62 PASLNIVPTETDLRIHQFVLSQ---NLVGLLIGKHGSFVTQIKAKTGASVYVR 211
PAS + P + LR+ V + N VG L+G G+ + +++A TG VY+R
Sbjct: 125 PASPSSYPVKRILRLDLPVDTYPNFNFVGRLLGPRGNSLKRVEATTGCRVYIR 177
>At1g14170.1 68414.m01675 KH domain-containing protein location of
EST 219C14T7 , gb|N38506
Length = 454
Score = 32.3 bits (70), Expect = 0.29
Identities = 14/32 (43%), Positives = 21/32 (65%)
Frame = +2
Query: 113 FVLSQNLVGLLIGKHGSFVTQIKAKTGASVYV 208
F+ VG +IGK G F+ QI+ +TGA++ V
Sbjct: 248 FICPAENVGGVIGKGGGFINQIRQETGATIRV 279
>At5g09560.1 68418.m01107 KH domain-containing protein various
predicted RNA binding proteins, Arabidopsis thaliana
Length = 563
Score = 31.9 bits (69), Expect = 0.38
Identities = 21/84 (25%), Positives = 38/84 (45%), Gaps = 1/84 (1%)
Frame = +2
Query: 11 EGSSDSGKGCSEAASPPPASLNIVPTETDLRIHQFVLSQNLVGLLIGKHGSFVTQIKAKT 190
EG + + A P P + P E + + + + G +IGKHG V ++ T
Sbjct: 2 EGKKFGAQSTARAPLPLPQPPSAPPPE--YVTFRILCNVSQAGHVIGKHGGMVKKLHKST 59
Query: 191 GASVYVRRHP-ESSKQKICAVEEH 259
+S++V + P + S +I + H
Sbjct: 60 ESSIWVEKTPLDDSPYRIIKIFGH 83
Score = 27.9 bits (59), Expect = 6.2
Identities = 15/52 (28%), Positives = 27/52 (51%)
Frame = +2
Query: 110 QFVLSQNLVGLLIGKHGSFVTQIKAKTGASVYVRRHPESSKQKICAVEEHKV 265
+ V+ N + L+G+ G T I+ +TGA + V ++ K C E ++V
Sbjct: 397 RLVVRSNQINCLLGEEGRIKTTIQQRTGAFITVLNVEQNPK---CVSENNQV 445
>At5g06770.1 68418.m00765 KH domain-containing protein / zinc finger
(CCCH type) family protein contains Pfam domains
PF00013: KH domain and PF00642: Zinc finger
C-x8-C-x5-C-x3-H type (and similar)
Length = 240
Score = 31.9 bits (69), Expect = 0.38
Identities = 22/81 (27%), Positives = 34/81 (41%)
Frame = +2
Query: 11 EGSSDSGKGCSEAASPPPASLNIVPTETDLRIHQFVLSQNLVGLLIGKHGSFVTQIKAKT 190
+GS G S P ++I T + + +L G +IGK G QI +T
Sbjct: 88 QGSGGPGGRFSGRGDPGSGPVSIFGASTS----KISVDASLAGAIIGKGGIHSKQICRET 143
Query: 191 GASVYVRRHPESSKQKICAVE 253
GA + ++ H KI +E
Sbjct: 144 GAKLSIKDHERDPNLKIIELE 164
>At5g46190.1 68418.m05681 KH domain-containing protein strong
similarity to unknown protein (pir||T04533)
Length = 644
Score = 31.5 bits (68), Expect = 0.51
Identities = 19/68 (27%), Positives = 32/68 (47%)
Frame = +2
Query: 50 ASPPPASLNIVPTETDLRIHQFVLSQNLVGLLIGKHGSFVTQIKAKTGASVYVRRHPESS 229
AS A+ N D R + ++ + ++IGK GS + I+ +T A+V V S
Sbjct: 133 ASLATAAENTKIDRDDFRECRLLVPSSQCSIVIGKSGSIIKNIRGRTRANVKVVSKDVSD 192
Query: 230 KQKICAVE 253
CA++
Sbjct: 193 PSHTCAMD 200
>At1g33680.1 68414.m04166 KH domain-containing protein similar to
FUSE binding protein 2 GB:AAC50892 GI:1575607 from [Homo
sapiens]
Length = 759
Score = 31.1 bits (67), Expect = 0.67
Identities = 13/31 (41%), Positives = 19/31 (61%)
Frame = +2
Query: 134 VGLLIGKHGSFVTQIKAKTGASVYVRRHPES 226
VG LIGK G V ++ +GA + +RR E+
Sbjct: 243 VGTLIGKGGEMVRYLQVNSGAKIQIRRDAEA 273
>At4g18375.2 68417.m02727 KH domain-containing protein contains
similarity to RNA-binding KH-domains PF:00013
Length = 606
Score = 29.9 bits (64), Expect = 1.5
Identities = 16/41 (39%), Positives = 22/41 (53%)
Frame = +2
Query: 143 LIGKHGSFVTQIKAKTGASVYVRRHPESSKQKICAVEEHKV 265
LIGK G + +I+ +T ASV V S +CA+E V
Sbjct: 153 LIGKAGENIKRIRRRTRASVKVVSKDVSDPSHVCAMEYDNV 193
>At4g18375.1 68417.m02726 KH domain-containing protein contains
similarity to RNA-binding KH-domains PF:00013
Length = 532
Score = 29.9 bits (64), Expect = 1.5
Identities = 16/41 (39%), Positives = 22/41 (53%)
Frame = +2
Query: 143 LIGKHGSFVTQIKAKTGASVYVRRHPESSKQKICAVEEHKV 265
LIGK G + +I+ +T ASV V S +CA+E V
Sbjct: 153 LIGKAGENIKRIRRRTRASVKVVSKDVSDPSHVCAMEYDNV 193
>At1g51580.1 68414.m05806 KH domain-containing protein
Length = 621
Score = 29.9 bits (64), Expect = 1.5
Identities = 14/39 (35%), Positives = 24/39 (61%)
Frame = +2
Query: 134 VGLLIGKHGSFVTQIKAKTGASVYVRRHPESSKQKICAV 250
VG LIGK G+ V ++ ++GAS+ V S+++I +
Sbjct: 287 VGSLIGKGGAVVRALQNESGASIKVSDPTHDSEERIIVI 325
Score = 28.7 bits (61), Expect = 3.6
Identities = 11/33 (33%), Positives = 22/33 (66%)
Frame = +2
Query: 134 VGLLIGKHGSFVTQIKAKTGASVYVRRHPESSK 232
+G L+GK G +++++ TGAS+ V +++K
Sbjct: 371 IGRLLGKGGHLISEMRRATGASIRVFAKDQATK 403
>At3g12130.1 68416.m01509 KH domain-containing protein / zinc finger
(CCCH type) family protein
Length = 248
Score = 29.5 bits (63), Expect = 2.0
Identities = 21/81 (25%), Positives = 34/81 (41%)
Frame = +2
Query: 11 EGSSDSGKGCSEAASPPPASLNIVPTETDLRIHQFVLSQNLVGLLIGKHGSFVTQIKAKT 190
+GS + G+ S P N + T +F + +L G +IGK G QI +T
Sbjct: 88 QGSGNGGRFSGRGESGPGHVSNFGDSATA----RFSVDASLAGAIIGKGGVSSKQICRQT 143
Query: 191 GASVYVRRHPESSKQKICAVE 253
G + ++ H K +E
Sbjct: 144 GVKLSIQDHERDPNLKNIVLE 164
>At5g51300.2 68418.m06360 splicing factor-related contains
similarity to SF1 protein [Drosophila melanogaster]
GI:6687400
Length = 804
Score = 28.3 bits (60), Expect = 4.7
Identities = 10/28 (35%), Positives = 19/28 (67%)
Frame = +2
Query: 128 NLVGLLIGKHGSFVTQIKAKTGASVYVR 211
N +GL+IG G+ +++ +TGA + +R
Sbjct: 256 NFIGLIIGPRGNTQKRMERETGAKIVIR 283
>At5g51300.1 68418.m06359 splicing factor-related contains
similarity to SF1 protein [Drosophila melanogaster]
GI:6687400
Length = 804
Score = 28.3 bits (60), Expect = 4.7
Identities = 10/28 (35%), Positives = 19/28 (67%)
Frame = +2
Query: 128 NLVGLLIGKHGSFVTQIKAKTGASVYVR 211
N +GL+IG G+ +++ +TGA + +R
Sbjct: 256 NFIGLIIGPRGNTQKRMERETGAKIVIR 283
>At5g56370.2 68418.m07037 F-box family protein contains F-box domain
Pfam:PF00646
Length = 421
Score = 27.9 bits (59), Expect = 6.2
Identities = 17/66 (25%), Positives = 27/66 (40%)
Frame = +1
Query: 355 YQKLTPLIPEFLQLQLVESVNNDTILTCLVSAGHFFLQQPLHPTFPR*RASRLWPHVQNP 534
Y+ + +PE ++ + + + D ILT L S L P P P H++
Sbjct: 232 YECMIGNLPEIVEAHVEVACSTDDILTSLASVKRLLLCLPTEPELPTGTIFHQLEHLE-- 289
Query: 535 VCRRCT 552
C CT
Sbjct: 290 FCSCCT 295
>At5g56370.1 68418.m07036 F-box family protein contains F-box domain
Pfam:PF00646
Length = 421
Score = 27.9 bits (59), Expect = 6.2
Identities = 17/66 (25%), Positives = 27/66 (40%)
Frame = +1
Query: 355 YQKLTPLIPEFLQLQLVESVNNDTILTCLVSAGHFFLQQPLHPTFPR*RASRLWPHVQNP 534
Y+ + +PE ++ + + + D ILT L S L P P P H++
Sbjct: 232 YECMIGNLPEIVEAHVEVACSTDDILTSLASVKRLLLCLPTEPELPTGTIFHQLEHLE-- 289
Query: 535 VCRRCT 552
C CT
Sbjct: 290 FCSCCT 295
>At5g23430.2 68418.m02749 transducin family protein / WD-40 repeat
family protein contains 6 WD-40 repeats (PF00400);
katanin p80 subunit (GI:3005601) [Strongylocentrotus
purpuratus]
Length = 836
Score = 27.9 bits (59), Expect = 6.2
Identities = 14/46 (30%), Positives = 28/46 (60%), Gaps = 2/46 (4%)
Frame = +2
Query: 125 QNLVGLLIGKHGSFVTQIKAKTGASVYVRRHPESS--KQKICAVEE 256
+N+V ++GKH FV+ ++++ VRR+ E + K I ++E+
Sbjct: 666 ENIVEDIMGKHNQFVSSMQSRLAKLQVVRRYWERNDVKNSIGSIEK 711
>At5g23430.1 68418.m02748 transducin family protein / WD-40 repeat
family protein contains 6 WD-40 repeats (PF00400);
katanin p80 subunit (GI:3005601) [Strongylocentrotus
purpuratus]
Length = 837
Score = 27.9 bits (59), Expect = 6.2
Identities = 14/46 (30%), Positives = 28/46 (60%), Gaps = 2/46 (4%)
Frame = +2
Query: 125 QNLVGLLIGKHGSFVTQIKAKTGASVYVRRHPESS--KQKICAVEE 256
+N+V ++GKH FV+ ++++ VRR+ E + K I ++E+
Sbjct: 666 ENIVEDIMGKHNQFVSSMQSRLAKLQVVRRYWERNDVKNSIGSIEK 711
>At2g34670.1 68415.m04259 proline-rich family protein contains
proline-rich region, INTERPRO:IPR000694
Length = 561
Score = 27.9 bits (59), Expect = 6.2
Identities = 22/78 (28%), Positives = 32/78 (41%), Gaps = 2/78 (2%)
Frame = +2
Query: 11 EGSSDSGKGCSEAASPPPA--SLNIVPTETDLRIHQFVLSQNLVGLLIGKHGSFVTQIKA 184
EG +D G S AA PPP S NI L +H N+V + K + Q +
Sbjct: 111 EGENDGGNDGSGAAPPPPLPNSWNIWNPFESLELHSHPNGDNVVTQVELKKKQQIQQAEE 170
Query: 185 KTGASVYVRRHPESSKQK 238
+ A + E +Q+
Sbjct: 171 EDWAETKSQFEEEDEQQE 188
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,651,024
Number of Sequences: 28952
Number of extensions: 275484
Number of successful extensions: 754
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 729
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 753
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1363910256
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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