Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= an--0104
(674 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g27470.1 68418.m03281 seryl-tRNA synthetase / serine--tRNA li... 213 9e-56
At1g11870.2 68414.m01369 seryl-tRNA synthetase, putative / serin... 122 2e-28
At1g11870.1 68414.m01368 seryl-tRNA synthetase, putative / serin... 122 2e-28
At1g11870.3 68414.m01367 seryl-tRNA synthetase, putative / serin... 38 0.005
At1g14850.1 68414.m01776 non-repetitive/WGA-negative nucleoporin... 31 0.53
At3g51980.1 68416.m05702 expressed protein 29 2.8
At2g47800.1 68415.m05966 glutathione-conjugate transporter (MRP4... 29 2.8
At4g05100.1 68417.m00758 myb family transcription factor (MYB74)... 29 3.8
At2g28120.1 68415.m03416 nodulin family protein similar to nodul... 29 3.8
At4g37650.1 68417.m05325 short-root transcription factor (SHR) 28 5.0
At5g18090.1 68418.m02124 transcriptional factor B3 family protei... 28 6.6
At3g10980.2 68416.m01324 expressed protein 28 6.6
At3g10980.1 68416.m01325 expressed protein 28 6.6
At5g53060.1 68418.m06592 KH domain-containing protein 27 8.7
At5g05350.1 68418.m00576 expressed protein contains Pfam profile... 27 8.7
At1g05930.1 68414.m00622 hypothetical protein contains Pfam prof... 27 8.7
>At5g27470.1 68418.m03281 seryl-tRNA synthetase / serine--tRNA
ligase identical to SP|Q39230 Seryl-tRNA synthetase (EC
6.1.1.11) (Serine--tRNA ligase) (SerRS) {Arabidopsis
thaliana}
Length = 451
Score = 213 bits (520), Expect = 9e-56
Identities = 97/165 (58%), Positives = 126/165 (76%), Gaps = 1/165 (0%)
Frame = -3
Query: 672 FRVHQFEKADQFVLTSPHDNASWQMMDEMINNAEEFCKALGLPYRVVNIVSGALNHAASK 493
FRVHQFEK +QF +T P++NASW+M+DEM+ N+E+F +AL LPY++V+IVSGALN AA+K
Sbjct: 283 FRVHQFEKIEQFCITGPNENASWEMLDEMMKNSEDFYQALKLPYQIVSIVSGALNDAAAK 342
Query: 492 KLDLEAWFPGSGAFRELVSCSNCLEYQARRLLVRYGQTKKMNAATEYVHMLNATMCATTR 313
K DLEAWFP S FRELVSCSNC +YQARRL +RYGQ K +YVHMLN+T+ AT R
Sbjct: 343 KYDLEAWFPSSETFRELVSCSNCTDYQARRLEIRYGQKKSNEQTKQYVHMLNSTLTATER 402
Query: 312 VICAILEVHQTEDGIKVPEILKPWLPKQYQELIPF-VKPAPIDVE 181
IC ILE +Q EDG+ +PE+L+P++ + +PF KP D +
Sbjct: 403 TICCILENYQREDGVDIPEVLQPFMGG--ETFLPFKAKPVVADTK 445
>At1g11870.2 68414.m01369 seryl-tRNA synthetase, putative /
serine--tRNA ligase, putative similar to PIR|T03949
serine--tRNA ligase (EC 6.1.1.11) serS {Zea mays};
contains Pfam profiles PF00587: tRNA synthetase class II
core domain (G, H, P, S and T), PF02403: Seryl-tRNA
synthetase N-terminal domain
Length = 514
Score = 122 bits (295), Expect = 2e-28
Identities = 67/156 (42%), Positives = 98/156 (62%), Gaps = 11/156 (7%)
Frame = -3
Query: 672 FRVHQFEKADQFVLTSPHDNASWQMMDEMINNAEEFCKALGLPYRVVNIVSGALNHAASK 493
+RVHQF KA+ FV+ P D+ S+ E+I E+ +LGL ++ +++ + L A +
Sbjct: 350 YRVHQFSKAEMFVICQPEDSESFHQ--ELIQIEEDLFTSLGLHFKTLDMATADLGAPAYR 407
Query: 492 KLDLEAWFPGSGAFRELVSCSNCLEYQARRLLVRY-----GQT--KKMNA---ATEYVHM 343
K D+EAW PG G F E+ S SNC +YQ+RRL +RY QT KK A AT++VH
Sbjct: 408 KFDIEAWMPGLGRFGEISSASNCTDYQSRRLGIRYRPSEPPQTGPKKGKANLPATKFVHT 467
Query: 342 LNATMCATTRVICAILEVHQTEDG-IKVPEILKPWL 238
LNAT CA R++ +LE +Q EDG + +PE L+P++
Sbjct: 468 LNATACAVPRMMVCLLENYQQEDGSVVIPEPLRPFM 503
>At1g11870.1 68414.m01368 seryl-tRNA synthetase, putative /
serine--tRNA ligase, putative similar to PIR|T03949
serine--tRNA ligase (EC 6.1.1.11) serS {Zea mays};
contains Pfam profiles PF00587: tRNA synthetase class II
core domain (G, H, P, S and T), PF02403: Seryl-tRNA
synthetase N-terminal domain
Length = 512
Score = 122 bits (295), Expect = 2e-28
Identities = 67/156 (42%), Positives = 98/156 (62%), Gaps = 11/156 (7%)
Frame = -3
Query: 672 FRVHQFEKADQFVLTSPHDNASWQMMDEMINNAEEFCKALGLPYRVVNIVSGALNHAASK 493
+RVHQF KA+ FV+ P D+ S+ E+I E+ +LGL ++ +++ + L A +
Sbjct: 348 YRVHQFSKAEMFVICQPEDSESFHQ--ELIQIEEDLFTSLGLHFKTLDMATADLGAPAYR 405
Query: 492 KLDLEAWFPGSGAFRELVSCSNCLEYQARRLLVRY-----GQT--KKMNA---ATEYVHM 343
K D+EAW PG G F E+ S SNC +YQ+RRL +RY QT KK A AT++VH
Sbjct: 406 KFDIEAWMPGLGRFGEISSASNCTDYQSRRLGIRYRPSEPPQTGPKKGKANLPATKFVHT 465
Query: 342 LNATMCATTRVICAILEVHQTEDG-IKVPEILKPWL 238
LNAT CA R++ +LE +Q EDG + +PE L+P++
Sbjct: 466 LNATACAVPRMMVCLLENYQQEDGSVVIPEPLRPFM 501
>At1g11870.3 68414.m01367 seryl-tRNA synthetase, putative /
serine--tRNA ligase, putative similar to PIR|T03949
serine--tRNA ligase (EC 6.1.1.11) serS {Zea mays};
contains Pfam profiles PF00587: tRNA synthetase class II
core domain (G, H, P, S and T), PF02403: Seryl-tRNA
synthetase N-terminal domain
Length = 402
Score = 38.3 bits (85), Expect = 0.005
Identities = 18/44 (40%), Positives = 27/44 (61%)
Frame = -3
Query: 672 FRVHQFEKADQFVLTSPHDNASWQMMDEMINNAEEFCKALGLPY 541
+RVHQF KA+ FV+ P D+ S+ E+I E+ +LGL +
Sbjct: 350 YRVHQFSKAEMFVICQPEDSESFH--QELIQIEEDLFTSLGLHF 391
>At1g14850.1 68414.m01776 non-repetitive/WGA-negative nucleoporin
family protein contains Pfam profile: PF03177
non-repetitive/WGA-negative nucleoporin
Length = 1464
Score = 31.5 bits (68), Expect = 0.53
Identities = 14/28 (50%), Positives = 18/28 (64%)
Frame = +1
Query: 244 RFEDFRYLDAVLCLVYLEYGANDPGGGA 327
RFED R+ +AV+CL + A DP G A
Sbjct: 932 RFEDLRFYEAVVCLPLQKAQALDPAGDA 959
>At3g51980.1 68416.m05702 expressed protein
Length = 382
Score = 29.1 bits (62), Expect = 2.8
Identities = 22/86 (25%), Positives = 37/86 (43%)
Frame = -3
Query: 591 EMINNAEEFCKALGLPYRVVNIVSGALNHAASKKLDLEAWFPGSGAFRELVSCSNCLEYQ 412
E I+NA + K+ GL +V+G LNH ++ L AW G + LE
Sbjct: 148 EPIDNANDLSKSGGL-----RVVAGELNHDDTEVRKLAAWVLGKASQNNPFVQEQVLELG 202
Query: 411 ARRLLVRYGQTKKMNAATEYVHMLNA 334
A L++ + A + + ++A
Sbjct: 203 ALTTLIKMVNSSSTEEAVKALFAVSA 228
>At2g47800.1 68415.m05966 glutathione-conjugate transporter (MRP4)
identical to AtMRP4 GI:2959767 from [Arabidopsis
thaliana]
Length = 1516
Score = 29.1 bits (62), Expect = 2.8
Identities = 15/36 (41%), Positives = 22/36 (61%)
Frame = +1
Query: 355 FSGSVHLFRLTVPYKQSSGLVLQTVTTRDKFPERTG 462
F G+VHL L V Y+ ++ LVL+ +T K E+ G
Sbjct: 1270 FHGNVHLEDLKVRYRPNTPLVLKGITLDIKGGEKVG 1305
>At4g05100.1 68417.m00758 myb family transcription factor (MYB74)
contains Pfam profile: PF00249 myb-like DNA-binding
domain; identical to cDNA putative transcription
factor (MYB74) mRNA, partial cds GI:3941505
Length = 324
Score = 28.7 bits (61), Expect = 3.8
Identities = 12/35 (34%), Positives = 17/35 (48%)
Frame = -2
Query: 346 HAERDHVRHHQGHLRHTRGTPDRGRHQGTGNPQTL 242
++ H HHQ H+ +R G HQ NP+ L
Sbjct: 146 NSSHHHHHHHQQHMNMSRLMMSDGNHQPLVNPEIL 180
Score = 28.3 bits (60), Expect = 5.0
Identities = 16/53 (30%), Positives = 30/53 (56%)
Frame = +2
Query: 437 ETSSLNAPDPGNQASKSSFLEAAWFRAPDTMFTTRYGSPSALQNSSALFIISS 595
+T+ N+ + SKS+F+ ++ ++ TT SP+ L +SS+ +I SS
Sbjct: 242 KTTVQNSLSYDDDCSKSNFVLEPYYSDFASVLTTPSSSPTPLNSSSSTYINSS 294
>At2g28120.1 68415.m03416 nodulin family protein similar to
nodulin-like protein [Arabidopsis thaliana] GI:3329368,
nodule-specific protein Nlj70 [Lotus japonicus]
GI:3329366
Length = 577
Score = 28.7 bits (61), Expect = 3.8
Identities = 19/64 (29%), Positives = 31/64 (48%)
Frame = -3
Query: 372 MNAATEYVHMLNATMCATTRVICAILEVHQTEDGIKVPEILKPWLPKQYQELIPFVKPAP 193
MN A + VH A A+ + CA+L V T + V + L+ W + +P +P+
Sbjct: 229 MNIAEKQVHFSKAAYAASATICCALLFVPLT---VSVKQELEVWNMMK----LPIEEPSE 281
Query: 192 IDVE 181
+ VE
Sbjct: 282 VKVE 285
>At4g37650.1 68417.m05325 short-root transcription factor (SHR)
Length = 531
Score = 28.3 bits (60), Expect = 5.0
Identities = 16/47 (34%), Positives = 29/47 (61%), Gaps = 2/47 (4%)
Frame = -3
Query: 612 ASWQMMDEMINNAEEFCKALGLPYRVVNIVS--GALNHAASKKLDLE 478
AS +MM E+ N E+F + +G+P++ NI+ G L+ +LD++
Sbjct: 303 ASHRMMKEIGNRMEKFARLMGVPFK-FNIIHHVGDLSEFDLNELDVK 348
>At5g18090.1 68418.m02124 transcriptional factor B3 family protein
contains Pfam profile PF02362: B3 DNA binding domain
Length = 301
Score = 27.9 bits (59), Expect = 6.6
Identities = 11/35 (31%), Positives = 16/35 (45%)
Frame = -3
Query: 666 VHQFEKADQFVLTSPHDNASWQMMDEMINNAEEFC 562
+H +A F + P SW +M + NN FC
Sbjct: 233 LHMPTEATMFKIHDPEGKRSWDVMYKFSNNQTRFC 267
>At3g10980.2 68416.m01324 expressed protein
Length = 286
Score = 27.9 bits (59), Expect = 6.6
Identities = 10/20 (50%), Positives = 14/20 (70%)
Frame = -3
Query: 528 IVSGALNHAASKKLDLEAWF 469
+++G LNHA KK +AWF
Sbjct: 148 VMTGMLNHALPKKSQRDAWF 167
>At3g10980.1 68416.m01325 expressed protein
Length = 557
Score = 27.9 bits (59), Expect = 6.6
Identities = 10/20 (50%), Positives = 14/20 (70%)
Frame = -3
Query: 528 IVSGALNHAASKKLDLEAWF 469
+++G LNHA KK +AWF
Sbjct: 148 VMTGMLNHALPKKSQRDAWF 167
>At5g53060.1 68418.m06592 KH domain-containing protein
Length = 652
Score = 27.5 bits (58), Expect = 8.7
Identities = 13/57 (22%), Positives = 26/57 (45%)
Frame = +1
Query: 298 YGANDPGGGAHGRVQHVHVFSGSVHLFRLTVPYKQSSGLVLQTVTTRDKFPERTGSW 468
Y GGG +GR H + + +R+ ++ G++ ++ T + TG+W
Sbjct: 44 YRGGGGGGGGNGRPSKSHPETMATTTYRILCHDAKAGGVIGKSGTIIKSIRQHTGAW 100
>At5g05350.1 68418.m00576 expressed protein contains Pfam profile
PF04749: Protein of unknown function, DUF614; expression
supported by MPSS
Length = 526
Score = 27.5 bits (58), Expect = 8.7
Identities = 12/42 (28%), Positives = 19/42 (45%)
Frame = -3
Query: 528 IVSGALNHAASKKLDLEAWFPGSGAFRELVSCSNCLEYQARR 403
+++G LNHA KK + WF + + CL +R
Sbjct: 128 VMTGMLNHALPKKSQRDVWFEVNNQILNALFTLMCLYQHPKR 169
>At1g05930.1 68414.m00622 hypothetical protein contains Pfam profile
PF03754: Domain of unknown function (DUF313)
Length = 318
Score = 27.5 bits (58), Expect = 8.7
Identities = 11/28 (39%), Positives = 19/28 (67%)
Frame = +2
Query: 413 WYSRQLLQETSSLNAPDPGNQASKSSFL 496
+Y + ++T ++N P+P +Q S SSFL
Sbjct: 101 YYMAEYYKKTETINPPNPYHQWSPSSFL 128
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,473,509
Number of Sequences: 28952
Number of extensions: 272461
Number of successful extensions: 817
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 779
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 810
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1432596384
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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