Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= P5PG0018
(792 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g05520.2 68417.m00839 calcium-binding EF hand family protein ... 175 3e-44
At4g05520.1 68417.m00838 calcium-binding EF hand family protein ... 175 3e-44
At3g20290.1 68416.m02571 calcium-binding EF hand family protein ... 175 4e-44
At3g46920.1 68416.m05092 protein kinase family protein similar t... 33 0.22
At1g04700.1 68414.m00467 protein kinase family protein low simil... 33 0.22
At2g35050.1 68415.m04300 protein kinase family protein contains ... 32 0.50
At1g79570.1 68414.m09276 protein kinase family protein low simil... 32 0.50
At1g16270.1 68414.m01948 protein kinase family protein contains ... 32 0.50
At3g24720.1 68416.m03104 protein kinase family protein protein k... 31 0.66
At5g57610.1 68418.m07197 protein kinase family protein similar t... 31 0.88
At3g57070.1 68416.m06354 glutaredoxin family protein contains Pf... 30 2.0
At4g35800.1 68417.m05087 DNA-directed RNA polymerase II largest ... 29 4.7
At5g40360.1 68418.m04896 myb family transcription factor (MYB115... 28 6.2
At4g33050.3 68417.m04705 calmodulin-binding family protein conta... 28 6.2
At4g33050.2 68417.m04703 calmodulin-binding family protein conta... 28 6.2
At4g33050.1 68417.m04704 calmodulin-binding family protein conta... 28 6.2
>At4g05520.2 68417.m00839 calcium-binding EF hand family protein
similar to EH-domain containing protein 1 from {Mus
musculus} SP|Q9WVK4, {Homo sapiens} SP|Q9H4M9,
receptor-mediated endocytosis 1 from [Caenorhabditis
elegans] GI:13487775, GI:13487777, GI:13487779; contains
INTERPRO:IPR002048 calcium-binding EF-hand domain
Length = 545
Score = 175 bits (426), Expect = 3e-44
Identities = 82/146 (56%), Positives = 102/146 (69%)
Frame = +2
Query: 353 GKTDIYSGICLERDFPGIRIGPEPTTDRFIAVMFDEKEGVIPGNALVVDPKKQFRPLSKF 532
GKT + L D+PG IGPEPTTDRF+ M E IPGN + V F L+ F
Sbjct: 208 GKTTFIKHL-LGCDYPGAHIGPEPTTDRFVVAMSGPDERTIPGNTMAVQADMPFNGLTSF 266
Query: 533 GNAFLNRFQCSTVTSDVLRGISIVDTPGILSGEKQRVDRGYDFTGVLEWFAERVDRIILL 712
G AFL++F+CS + VL I++VDTPG+LSGEKQR+ R YDFTGV+ WFA + D I+LL
Sbjct: 267 GGAFLSKFECSQMPHPVLDQITLVDTPGVLSGEKQRMQRSYDFTGVISWFASKCDMILLL 326
Query: 713 FDAHKLDISDEFRRSIEALRGHDDKI 790
FD HKLDISDEF+R I +LRG++DKI
Sbjct: 327 FDPHKLDISDEFKRVITSLRGNEDKI 352
>At4g05520.1 68417.m00838 calcium-binding EF hand family protein
similar to EH-domain containing protein 1 from {Mus
musculus} SP|Q9WVK4, {Homo sapiens} SP|Q9H4M9,
receptor-mediated endocytosis 1 from [Caenorhabditis
elegans] GI:13487775, GI:13487777, GI:13487779; contains
INTERPRO:IPR002048 calcium-binding EF-hand domain
Length = 546
Score = 175 bits (426), Expect = 3e-44
Identities = 82/146 (56%), Positives = 102/146 (69%)
Frame = +2
Query: 353 GKTDIYSGICLERDFPGIRIGPEPTTDRFIAVMFDEKEGVIPGNALVVDPKKQFRPLSKF 532
GKT + L D+PG IGPEPTTDRF+ M E IPGN + V F L+ F
Sbjct: 209 GKTTFIKHL-LGCDYPGAHIGPEPTTDRFVVAMSGPDERTIPGNTMAVQADMPFNGLTSF 267
Query: 533 GNAFLNRFQCSTVTSDVLRGISIVDTPGILSGEKQRVDRGYDFTGVLEWFAERVDRIILL 712
G AFL++F+CS + VL I++VDTPG+LSGEKQR+ R YDFTGV+ WFA + D I+LL
Sbjct: 268 GGAFLSKFECSQMPHPVLDQITLVDTPGVLSGEKQRMQRSYDFTGVISWFASKCDMILLL 327
Query: 713 FDAHKLDISDEFRRSIEALRGHDDKI 790
FD HKLDISDEF+R I +LRG++DKI
Sbjct: 328 FDPHKLDISDEFKRVITSLRGNEDKI 353
>At3g20290.1 68416.m02571 calcium-binding EF hand family protein
similar to EH-domain containing protein 1 from {Mus
musculus} SP|Q9WVK4 and {Homo sapiens} SP|Q9H4M9,
receptor-mediated endocytosis 1 from [Caenorhabditis
elegans] GI:13487775, GI:13487777, GI:13487779; contains
INTERPRO:IPR002048 calcium-binding EF-hand domain
Length = 545
Score = 175 bits (425), Expect = 4e-44
Identities = 81/146 (55%), Positives = 100/146 (68%)
Frame = +2
Query: 353 GKTDIYSGICLERDFPGIRIGPEPTTDRFIAVMFDEKEGVIPGNALVVDPKKQFRPLSKF 532
GKT + L+ +PG IGPEPTTDRF+ VM E IPGN + V F L+ F
Sbjct: 209 GKTTFIKHL-LKSTYPGAHIGPEPTTDRFVVVMSGPDERSIPGNTVAVQADMPFSGLTTF 267
Query: 533 GNAFLNRFQCSTVTSDVLRGISIVDTPGILSGEKQRVDRGYDFTGVLEWFAERVDRIILL 712
G AFL++F+CS + +L ++ VDTPG+LSGEKQR R YDFTGV WFA + D I+LL
Sbjct: 268 GTAFLSKFECSQMPHPLLEHVTFVDTPGVLSGEKQRTQRAYDFTGVTSWFASKCDLILLL 327
Query: 713 FDAHKLDISDEFRRSIEALRGHDDKI 790
FD HKLD+SDEF+R I +LRGHDDKI
Sbjct: 328 FDPHKLDVSDEFKRVISSLRGHDDKI 353
>At3g46920.1 68416.m05092 protein kinase family protein similar to
MAP3K delta-1 protein kinase [Arabidopsis thaliana]
GI:2253010; contains Pfam profile: PF00069 Eukaryotic
protein kinase domain
Length = 1171
Score = 33.1 bits (72), Expect = 0.22
Identities = 20/66 (30%), Positives = 35/66 (53%), Gaps = 2/66 (3%)
Frame = +2
Query: 488 LVVDPKKQFRPLSKFGNAFLNRFQCSTVTSDVLRGISIVDTPGILSGEKQRVDRGYD-FT 664
L+V+ + RP+ K G+ L++ +C T+ S +RG P +L+G V D F+
Sbjct: 1023 LLVNLRDPHRPICKVGDLGLSKVKCQTLISGGVRGTLPWMAPELLNGTSSLVSEKVDVFS 1082
Query: 665 -GVLEW 679
G++ W
Sbjct: 1083 FGIVLW 1088
>At1g04700.1 68414.m00467 protein kinase family protein low similarity
to EDR1 [Arabidopsis thaliana] GI:11127925; contains Pfam
profile: PF00069 Eukaryotic protein kinase domain
Length = 1042
Score = 33.1 bits (72), Expect = 0.22
Identities = 21/66 (31%), Positives = 36/66 (54%), Gaps = 2/66 (3%)
Frame = +2
Query: 488 LVVDPKKQFRPLSKFGNAFLNRFQCSTVTSDVLRGISIVDTPGILSGEKQRVDRGYD-FT 664
L+V+ + RP+ K G+ L+R + +T+ S +RG P +L+G RV D F+
Sbjct: 901 LLVNLRDPQRPICKVGDFGLSRIKRNTLVSGGVRGTLPWMAPELLNGSSNRVSEKVDVFS 960
Query: 665 -GVLEW 679
G++ W
Sbjct: 961 FGIVMW 966
>At2g35050.1 68415.m04300 protein kinase family protein contains Pfam
profile: PF00069 Eukaryotic protein kinase domain
Length = 1257
Score = 31.9 bits (69), Expect = 0.50
Identities = 20/66 (30%), Positives = 36/66 (54%), Gaps = 2/66 (3%)
Frame = +2
Query: 488 LVVDPKKQFRPLSKFGNAFLNRFQCSTVTSDVLRGISIVDTPGILSGEKQRVDRGYD-FT 664
L+V+ K RP+ K G+ L++ + +T+ + +RG P +LSG +V D F+
Sbjct: 1108 LLVNLKDPARPICKVGDFGLSKIKRNTLVTGGVRGTLPWMAPELLSGSSSKVSEKVDVFS 1167
Query: 665 -GVLEW 679
G++ W
Sbjct: 1168 FGIVLW 1173
>At1g79570.1 68414.m09276 protein kinase family protein low similarity
to EDR1 [Arabidopsis thaliana] GI:11127925
Length = 1248
Score = 31.9 bits (69), Expect = 0.50
Identities = 20/66 (30%), Positives = 36/66 (54%), Gaps = 2/66 (3%)
Frame = +2
Query: 488 LVVDPKKQFRPLSKFGNAFLNRFQCSTVTSDVLRGISIVDTPGILSGEKQRVDRGYD-FT 664
L+V+ K RP+ K G+ L++ + +T+ S +RG P +L+G +V D F+
Sbjct: 1099 LLVNLKDPSRPICKVGDFGLSKIKRNTLVSGGVRGTLPWMAPELLNGSSSKVSEKVDVFS 1158
Query: 665 -GVLEW 679
G++ W
Sbjct: 1159 FGIVLW 1164
>At1g16270.1 68414.m01948 protein kinase family protein contains
PF|00069 Eukaryotic protein kinase domain. ESTs
gb|H37741, gb|T43005 and gb|AI100340 come from this gene
Length = 1147
Score = 31.9 bits (69), Expect = 0.50
Identities = 20/66 (30%), Positives = 36/66 (54%), Gaps = 2/66 (3%)
Frame = +2
Query: 488 LVVDPKKQFRPLSKFGNAFLNRFQCSTVTSDVLRGISIVDTPGILSGEKQRVDRGYD-FT 664
L+V+ K RP+ K G+ L++ + +T+ S +RG P +L+G +V D F+
Sbjct: 998 LLVNLKDPSRPICKVGDFGLSKIKRNTLVSGGVRGTLPWMAPELLNGSSSKVSEKVDVFS 1057
Query: 665 -GVLEW 679
G++ W
Sbjct: 1058 FGIVLW 1063
>At3g24720.1 68416.m03104 protein kinase family protein protein
kinase family; similar to tyrosine-protein kinase
GB:P18160 from [Dictyostelium discoideum]
Length = 297
Score = 31.5 bits (68), Expect = 0.66
Identities = 18/57 (31%), Positives = 30/57 (52%)
Frame = +2
Query: 488 LVVDPKKQFRPLSKFGNAFLNRFQCSTVTSDVLRGISIVDTPGILSGEKQRVDRGYD 658
L+V+ + RP+ K G+ L+R + +T+ S +RG P +L+G RV D
Sbjct: 151 LLVNLRDPQRPICKVGDLGLSRIKRNTLVSGGVRGTLPWMAPELLNGSSTRVSEKVD 207
>At5g57610.1 68418.m07197 protein kinase family protein similar to
protein kinase [Glycine max] GI:170047, MAP3K delta-1
protein kinase [Arabidopsis thaliana] GI:2253010;
contains Pfam profile: PF00069 Eukaryotic protein kinase
domain
Length = 1054
Score = 31.1 bits (67), Expect = 0.88
Identities = 19/66 (28%), Positives = 33/66 (50%), Gaps = 2/66 (3%)
Frame = +2
Query: 488 LVVDPKKQFRPLSKFGNAFLNRFQCSTVTSDVLRGISIVDTPGILSGEKQRVDRGYDF-- 661
L+V+ + RP+ K G+ L++ + T+ S +RG P +LSG+ V D
Sbjct: 916 LLVNMRDPQRPICKIGDLGLSKVKQKTLVSGGVRGTLPWMAPELLSGKSNMVSEKIDVYS 975
Query: 662 TGVLEW 679
G++ W
Sbjct: 976 FGIVMW 981
>At3g57070.1 68416.m06354 glutaredoxin family protein contains Pfam
profile PF00462: Glutaredoxin
Length = 417
Score = 29.9 bits (64), Expect = 2.0
Identities = 19/56 (33%), Positives = 27/56 (48%), Gaps = 1/56 (1%)
Frame = -1
Query: 741 SDIS-SL*ASNKSMIRSTRSANHSNTPVKSYPRSTRCFSPDRIPGVSTIEIPRSTS 577
SD+ S+ +S K + S +NHSNT P + SP P + E P+S S
Sbjct: 195 SDLDPSIVSSYKKALSSKLLSNHSNTRNPHRPTKSLSCSPSSNPSILISEEPKSVS 250
>At4g35800.1 68417.m05087 DNA-directed RNA polymerase II largest
subunit (RPB205) (RPII) (RPB1) nearly identical to
P|P18616 DNA-directed RNA polymerase II largest subunit
(EC 2.7.7.6) {Arabidopsis thaliana}
Length = 1840
Score = 28.7 bits (61), Expect = 4.7
Identities = 17/63 (26%), Positives = 27/63 (42%)
Frame = +2
Query: 500 PKKQFRPLSKFGNAFLNRFQCSTVTSDVLRGISIVDTPGILSGEKQRVDRGYDFTGVLEW 679
PK + + S + FL + + + +T LRGI ++ I K R D F EW
Sbjct: 1267 PKGELQDESAEDDVFLKKIESNMLTEMALRGIPDINKVFIKQVRKSRFDEEGGFKTSEEW 1326
Query: 680 FAE 688
+
Sbjct: 1327 MLD 1329
>At5g40360.1 68418.m04896 myb family transcription factor (MYB115)
contains Pfam profile: PF00249 myb-like DNA-binding
domain
Length = 359
Score = 28.3 bits (60), Expect = 6.2
Identities = 13/52 (25%), Positives = 24/52 (46%)
Frame = -1
Query: 594 IPRSTSDVTVEHWNRFRKALPNLLNGRNCFFGSTTNALPGITPSFSSNITAI 439
+P + ++ HWN ++ L ++ R+ F NAL S + N A+
Sbjct: 239 LPGRSENIVKNHWNATKRRLHSVRTKRSDAFSPRNNALENYIRSITINNNAL 290
>At4g33050.3 68417.m04705 calmodulin-binding family protein contains
Pfam profile PF00612: IQ calmodulin-binding motif
Length = 295
Score = 28.3 bits (60), Expect = 6.2
Identities = 12/30 (40%), Positives = 17/30 (56%)
Frame = -1
Query: 597 EIPRSTSDVTVEHWNRFRKALPNLLNGRNC 508
E+P+ T + +K+ PN LNGRNC
Sbjct: 50 EVPKETKTDSDFEVLETKKSTPNTLNGRNC 79
>At4g33050.2 68417.m04703 calmodulin-binding family protein contains
Pfam profile PF00612: IQ calmodulin-binding motif
Length = 526
Score = 28.3 bits (60), Expect = 6.2
Identities = 12/30 (40%), Positives = 17/30 (56%)
Frame = -1
Query: 597 EIPRSTSDVTVEHWNRFRKALPNLLNGRNC 508
E+P+ T + +K+ PN LNGRNC
Sbjct: 50 EVPKETKTDSDFEVLETKKSTPNTLNGRNC 79
>At4g33050.1 68417.m04704 calmodulin-binding family protein contains
Pfam profile PF00612: IQ calmodulin-binding motif
Length = 374
Score = 28.3 bits (60), Expect = 6.2
Identities = 12/30 (40%), Positives = 17/30 (56%)
Frame = -1
Query: 597 EIPRSTSDVTVEHWNRFRKALPNLLNGRNC 508
E+P+ T + +K+ PN LNGRNC
Sbjct: 50 EVPKETKTDSDFEVLETKKSTPNTLNGRNC 79
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,286,324
Number of Sequences: 28952
Number of extensions: 330378
Number of successful extensions: 872
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 856
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 872
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1785055200
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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