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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060139.seq
         (660 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g22845.1 68416.m02879 emp24/gp25L/p24 protein-related contain...    37   0.014
At4g10360.1 68417.m01701 expressed protein                             32   0.39 
At3g02430.1 68416.m00230 hypothetical protein contains Pfam prof...    29   3.6  
At3g10780.1 68416.m01298 emp24/gp25L/p24 family protein similar ...    28   6.3  
At1g06660.1 68414.m00706 expressed protein                             28   6.3  

>At3g22845.1 68416.m02879 emp24/gp25L/p24 protein-related contains
           weak similarity to transmembrane protein (GI:1212965)
           [Homo sapiens]
          Length = 214

 Score = 36.7 bits (81), Expect = 0.014
 Identities = 39/141 (27%), Positives = 59/141 (41%), Gaps = 7/141 (4%)
 Frame = +3

Query: 108 LLNLVSNAYSYTVTVDAHAEECFYENV--EADTKLGLSFEIAEGGFLDID---V*Y*ALM 272
           LLN ++   S +VTV+   EEC  E V  E DT  G    +    F   D   + +    
Sbjct: 18  LLNSINQISSLSVTVND--EECVQEYVLYEGDTVSGNFVVVDHDIFWGSDHPGLDFTVTS 75

Query: 273 ATSFIKQ--RGSHLVFIHFSAPTAGKYTYCFSNKMSSMTPKVVMFNLEIGEPQTKSGNEN 446
               I Q  +G+      F AP +G Y +CF N  S  TP+ V F + +G    +     
Sbjct: 76  PAGNIVQTLKGTSGDKFEFKAPKSGMYKFCFHNPYS--TPETVSFYIHVGHIPNEHDLAK 133

Query: 447 EADHNKLEDMIKELATTLRTI 509
           +   + +   I EL   L ++
Sbjct: 134 DEHLDPVNVKIAELREALESV 154



 Score = 32.3 bits (70), Expect = 0.29
 Identities = 12/26 (46%), Positives = 18/26 (69%)
 Frame = +2

Query: 515 DQEYMQVRDRIHRSINESTNSRVVMW 592
           +Q+Y++ RD  HR  NEST  RV+ +
Sbjct: 157 EQKYLKARDTRHRHTNESTRKRVIFY 182


>At4g10360.1 68417.m01701 expressed protein
          Length = 266

 Score = 31.9 bits (69), Expect = 0.39
 Identities = 24/93 (25%), Positives = 44/93 (47%)
 Frame = -2

Query: 590 TSPHVNLYFHLLNDGYDHAPAYTLDRVYSS*CSSKFFDHVLKFIMVSFIFITRFSLWFSD 411
           T+P VNL ++L N G   + AYTL+ +      + F   ++  +++   F     L F  
Sbjct: 164 TTPFVNLRWYLDNSGQKGSKAYTLNGI------ALFLGWLVARVLLFIFFFVHMYLHFHQ 217

Query: 410 FKIEHYHLGCHG*HFITEAISILPCSWSRKMYK 312
            K + + LG +    I  A++++   W  K+ K
Sbjct: 218 VK-QVFPLGFYSLLTIPPALAVMNLLWFWKITK 249


>At3g02430.1 68416.m00230 hypothetical protein contains Pfam profile
           PF05078: Protein of unknown function (DUF679)
          Length = 219

 Score = 28.7 bits (61), Expect = 3.6
 Identities = 17/45 (37%), Positives = 24/45 (53%), Gaps = 1/45 (2%)
 Frame = +3

Query: 72  KY-LRISQWLVVTLLNLVSNAYSYTVTVDAHAEECFYENVEADTK 203
           KY +R   W+  TL  LV  A +     D +  +CFY + EA+TK
Sbjct: 139 KYRMRFVDWIHATLSVLVFGAVALR---DKYITDCFYPSPEAETK 180


>At3g10780.1 68416.m01298 emp24/gp25L/p24 family protein similar to
           SP|O35587 Transmembrane protein Tmp21 precursor (21 kDa
           Transmembrane trafficking protein) {Mesocricetus
           auratus}; contains Pfam profile: PF01105 emp24/gp25L/p24
           family; contains non-consensus TG acceptor splice site
           at exon 3
          Length = 217

 Score = 27.9 bits (59), Expect = 6.3
 Identities = 12/35 (34%), Positives = 21/35 (60%)
 Frame = +2

Query: 479 QRTCYYIKNYKHDQEYMQVRDRIHRSINESTNSRV 583
           +R+  Y    + +  Y+++R+   R INE TN+RV
Sbjct: 151 RRSTEYASAIRANILYLRIREAYMREINEKTNTRV 185


>At1g06660.1 68414.m00706 expressed protein
          Length = 481

 Score = 27.9 bits (59), Expect = 6.3
 Identities = 14/40 (35%), Positives = 22/40 (55%)
 Frame = +3

Query: 81  RISQWLVVTLLNLVSNAYSYTVTVDAHAEECFYENVEADT 200
           RI    V ++ N++ NA  Y V  D+H    + E +EA+T
Sbjct: 290 RIRSQFVHSVSNIMENASLYKVYKDSHEGLDYEEQIEAET 329


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,593,178
Number of Sequences: 28952
Number of extensions: 271502
Number of successful extensions: 688
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 672
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 688
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1383534864
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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