Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060117.seq
(559 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g63840.1 68418.m08014 alpha-glucosidase, putative similar to ... 106 9e-24
At3g23640.1 68416.m02973 glycosyl hydrolase family 31 protein si... 84 7e-17
At5g11720.1 68418.m01369 alpha-glucosidase 1 (AGLU1) identical t... 67 9e-12
At1g68560.1 68414.m07833 alpha-xylosidase (XYL1) identical to al... 64 8e-11
At3g45940.1 68416.m04971 alpha-xylosidase, putative strong simil... 63 1e-10
At3g05430.1 68416.m00595 PWWP domain-containing protein contains... 30 0.91
At4g38220.2 68417.m05395 aminoacylase, putative / N-acyl-L-amino... 29 1.6
At4g38220.1 68417.m05394 aminoacylase, putative / N-acyl-L-amino... 29 1.6
At5g04410.1 68418.m00433 no apical meristem (NAM) family protein... 29 2.1
At5g67300.1 68418.m08486 myb family transcription factor contain... 29 2.8
At3g10500.1 68416.m01260 no apical meristem (NAM) family protein... 29 2.8
At2g33855.1 68415.m04156 expressed protein 29 2.8
At5g41315.1 68418.m05021 basic helix-loop-helix (bHLH) family pr... 28 3.7
At3g01420.1 68416.m00065 pathogen-responsive alpha-dioxygenase, ... 27 6.4
At1g63650.2 68414.m07202 basic helix-loop-helix (bHLH) family pr... 27 6.4
At1g63650.1 68414.m07201 basic helix-loop-helix (bHLH) family pr... 27 6.4
At5g37340.2 68418.m04485 zinc finger (ZPR1-type) family protein ... 27 8.5
At5g37340.1 68418.m04484 zinc finger (ZPR1-type) family protein ... 27 8.5
At3g23480.1 68416.m02958 cyclopropane fatty acid synthase-relate... 27 8.5
At1g17760.1 68414.m02198 suppressor of forked protein family pro... 27 8.5
>At5g63840.1 68418.m08014 alpha-glucosidase, putative similar to
alpha-glucosidase GI:2648032 from [Solanum tuberosum]
Length = 921
Score = 106 bits (255), Expect = 9e-24
Identities = 47/100 (47%), Positives = 63/100 (63%)
Frame = +1
Query: 256 GFFKYPEAELMTRWYQAAAFQPFFRAHSHIETKRREPWLXXDGTTVLIRDANRKRYALLD 435
GFF PE EL+ RWYQ A+ PFFR H+H +TKRREPWL + T L+RDA RY LL
Sbjct: 620 GFFGNPEPELLVRWYQVGAYYPFFRGHAHHDTKRREPWLFGERNTELMRDAIHTRYTLLP 679
Query: 436 XWYTLXYEHTVDGLTXMRPLFQXYLDXPVTYXIDDQYLLG 555
+YTL E V G+ +RPL+ + T+ D+ +++G
Sbjct: 680 YFYTLFREANVTGVPVVRPLWMEFPQDEATFSNDEAFMVG 719
Score = 105 bits (253), Expect = 2e-23
Identities = 46/82 (56%), Positives = 59/82 (71%)
Frame = +2
Query: 8 EHRHVHNEYGLWNLRATNTGLLDRADGVYRPFLLTRAXFAGTQRYSAVWTGDNTAEWSFL 187
EHR VHN YG + AT+ GL+ R +G RPF+L+RA F GTQRY A+WTGDNTAEW L
Sbjct: 537 EHREVHNAYGYYFHMATSDGLVMREEGKDRPFVLSRAIFPGTQRYGAIWTGDNTAEWEHL 596
Query: 188 AASVPMCLSLAIAGNSFCGSDV 253
S+PM L+L + G +F G+D+
Sbjct: 597 RVSIPMILTLGLTGITFSGADI 618
>At3g23640.1 68416.m02973 glycosyl hydrolase family 31 protein
similar to alpha-glucosidase II from SP:Q9F234 [Bacillus
thermoamyloliquefaciens]; contains Pfam profile: PF01055
Glycosyl hydrolases family 31
Length = 991
Score = 83.8 bits (198), Expect = 7e-17
Identities = 38/81 (46%), Positives = 50/81 (61%)
Frame = +2
Query: 11 HRHVHNEYGLWNLRATNTGLLDRADGVYRPFLLTRAXFAGTQRYSAVWTGDNTAEWSFLA 190
H H HN YG+ R+T G+ + AD RPF+LTRA F G+QRY+A WTGDN + W L
Sbjct: 377 HSHYHNVYGMLMARSTYEGM-ELADKNKRPFVLTRAGFIGSQRYAATWTGDNLSNWEHLH 435
Query: 191 ASVPMCLSLAIAGNSFCGSDV 253
S+ M L L ++G G D+
Sbjct: 436 MSISMVLQLGLSGQPLSGPDI 456
Score = 51.2 bits (117), Expect = 5e-07
Identities = 28/81 (34%), Positives = 34/81 (41%)
Frame = +1
Query: 256 GFFKYPEAELMTRWYQAAAFQPFFRAHSHIETKRREPWLXXDGTTVLIRDANRKRYALLD 435
GF L RW A PF R HS T EPW + + R A ++RY LL
Sbjct: 458 GFAGNATPRLFGRWMGVGAMFPFCRGHSEAGTDDHEPWSFGEECEEVCRAALKRRYQLLP 517
Query: 436 XWYTLXYEHTVDGLTXMRPLF 498
+YTL Y G P+F
Sbjct: 518 HFYTLFYIAHTTGAPVAAPIF 538
>At5g11720.1 68418.m01369 alpha-glucosidase 1 (AGLU1) identical to
alpha-glucosidase 1 [Arabidopsis thaliana] GI:2323344
Length = 902
Score = 66.9 bits (156), Expect = 9e-12
Identities = 31/77 (40%), Positives = 47/77 (61%)
Frame = +2
Query: 23 HNEYGLWNLRATNTGLLDRADGVYRPFLLTRAXFAGTQRYSAVWTGDNTAEWSFLAASVP 202
HN YGL +AT+ ++D RPF+L+R+ F + +Y+A WTGDN A+W LA S+P
Sbjct: 514 HNLYGLLEAKATHQAVVDITGK--RPFILSRSTFVSSGKYTAHWTGDNAAKWEDLAYSIP 571
Query: 203 MCLSLAIAGNSFCGSDV 253
L+ + G G+D+
Sbjct: 572 GILNFGLFGIPMVGADI 588
Score = 66.9 bits (156), Expect = 9e-12
Identities = 41/100 (41%), Positives = 47/100 (47%)
Frame = +1
Query: 256 GFFKYPEAELMTRWYQAAAFQPFFRAHSHIETKRREPWLXXDGTTVLIRDANRKRYALLD 435
GF EL RW Q AF PF R HS + T R+E +L D R R LL
Sbjct: 590 GFSHDTTEELCRRWIQLGAFYPFARDHSSLGTARQELYLW-DSVASSARKVLGLRMRLLP 648
Query: 436 XWYTLXYEHTVDGLTXMRPLFQXYLDXPVTYXIDDQYLLG 555
YTL YE V G RPLF + TY ID Q+L+G
Sbjct: 649 HLYTLMYEAHVSGNPIARPLFFSFPQDTKTYEIDSQFLIG 688
>At1g68560.1 68414.m07833 alpha-xylosidase (XYL1) identical to
alpha-xylosidase precursor GB:AAD05539 GI:4163997 from
[Arabidopsis thaliana]; contains Pfam profile PF01055:
Glycosyl hydrolases family 31; identical to cDNA
alpha-xylosidase precursor (XYL1) partial cds GI:4163996
Length = 915
Score = 63.7 bits (148), Expect = 8e-11
Identities = 36/100 (36%), Positives = 49/100 (49%)
Frame = +1
Query: 256 GFFKYPEAELMTRWYQAAAFQPFFRAHSHIETKRREPWLXXDGTTVLIRDANRKRYALLD 435
GF+ P EL RW + AF PF R H++ + R+E + D R+A RY +L
Sbjct: 595 GFYPQPTEELCNRWIEVGAFYPFSRDHANYYSPRQELY-QWDTVADSARNALGMRYKILP 653
Query: 436 XWYTLXYEHTVDGLTXMRPLFQXYLDXPVTYXIDDQYLLG 555
YTL YE + G RPLF + + Y Q+LLG
Sbjct: 654 FLYTLNYEAHMTGAPIARPLFFSFPEYTECYGNSRQFLLG 693
Score = 62.1 bits (144), Expect = 2e-10
Identities = 32/77 (41%), Positives = 44/77 (57%)
Frame = +2
Query: 23 HNEYGLWNLRATNTGLLDRADGVYRPFLLTRAXFAGTQRYSAVWTGDNTAEWSFLAASVP 202
H+ YG AT+ GLL+ G RPF+L+R+ F G+ +Y+A WTGDN W L S+
Sbjct: 519 HSIYGFSETIATHKGLLN-VQGK-RPFILSRSTFVGSGQYAAHWTGDNQGTWQSLQVSIS 576
Query: 203 MCLSLAIAGNSFCGSDV 253
L+ I G GSD+
Sbjct: 577 TMLNFGIFGVPMVGSDI 593
>At3g45940.1 68416.m04971 alpha-xylosidase, putative strong
similarity to alpha-xylosidase precursor GI:4163997 from
[Arabidopsis thaliana]
Length = 868
Score = 62.9 bits (146), Expect = 1e-10
Identities = 39/101 (38%), Positives = 48/101 (47%), Gaps = 1/101 (0%)
Frame = +1
Query: 256 GFFKYPEAELMTRWYQAAAFQPFFRAHSHIETKRREPWLXXDGTTV-LIRDANRKRYALL 432
GFF EL RW + AF PF R H+ R+E L GT R+A RY LL
Sbjct: 547 GFFPPTPEELCNRWIEVGAFYPFSRDHADYYAPRKE--LYQWGTVAESARNALGMRYKLL 604
Query: 433 DXWYTLXYEHTVDGLTXMRPLFQXYLDXPVTYXIDDQYLLG 555
YTL YE + G RPLF + + Y + Q+LLG
Sbjct: 605 PFLYTLNYEAHMSGAPIARPLFFSFPEFTECYGLSKQFLLG 645
Score = 58.8 bits (136), Expect = 2e-09
Identities = 31/77 (40%), Positives = 42/77 (54%)
Frame = +2
Query: 23 HNEYGLWNLRATNTGLLDRADGVYRPFLLTRAXFAGTQRYSAVWTGDNTAEWSFLAASVP 202
H+ YG AT+ LL A RPF+L+R+ F G+ +Y+A WTGDN W L S+
Sbjct: 471 HSIYGFSEAIATHKALL--AVQGKRPFILSRSTFVGSGQYAAHWTGDNQGTWQSLQVSIS 528
Query: 203 MCLSLAIAGNSFCGSDV 253
L+ I G GSD+
Sbjct: 529 TMLNFGIFGVPMVGSDI 545
>At3g05430.1 68416.m00595 PWWP domain-containing protein contains
Pfam profile:PF00855 PWWP domain
Length = 965
Score = 30.3 bits (65), Expect = 0.91
Identities = 20/69 (28%), Positives = 31/69 (44%)
Frame = -2
Query: 231 FPAIASDKHIGTEAARNDHSAVLSPVHTAEYLWVPANXALVSRKGL*TPSARSSSPVLVA 52
F +ASDK +A + A L+PV + + A A+V ++ PSA P ++
Sbjct: 687 FKPLASDKKTNQDATKRSSLAPLNPVRDQCRVPINAKPAIVQQEKKTGPSAMVVEPTMLV 746
Query: 51 RRFHSPYSL 25
F SL
Sbjct: 747 MMFPPGESL 755
>At4g38220.2 68417.m05395 aminoacylase, putative /
N-acyl-L-amino-acid amidohydrolase, putative similar to
aminoacylase-1 (N-acyl-L-amino-acid amidohydrolase,
ACY-1)[Homo sapiens] SWISS-PROT:Q03154
Length = 433
Score = 29.5 bits (63), Expect = 1.6
Identities = 19/43 (44%), Positives = 23/43 (53%), Gaps = 3/43 (6%)
Frame = -2
Query: 417 LAVGVPNEHGGAVXVQPGLAPLGLDVRVGPK---ERLERGRLV 298
L G P+ G + +QP A G D+RV P E LER RLV
Sbjct: 267 LKAGTPSPTGFVMNLQPSEAEAGFDIRVPPSVDAEALER-RLV 308
>At4g38220.1 68417.m05394 aminoacylase, putative /
N-acyl-L-amino-acid amidohydrolase, putative similar to
aminoacylase-1 (N-acyl-L-amino-acid amidohydrolase,
ACY-1)[Homo sapiens] SWISS-PROT:Q03154
Length = 430
Score = 29.5 bits (63), Expect = 1.6
Identities = 19/43 (44%), Positives = 23/43 (53%), Gaps = 3/43 (6%)
Frame = -2
Query: 417 LAVGVPNEHGGAVXVQPGLAPLGLDVRVGPK---ERLERGRLV 298
L G P+ G + +QP A G D+RV P E LER RLV
Sbjct: 267 LKAGTPSPTGFVMNLQPSEAEAGFDIRVPPSVDAEALER-RLV 308
>At5g04410.1 68418.m00433 no apical meristem (NAM) family protein
contains Pfam PF02365: No apical meristem (NAM) protein;
supporting cDNA gi|6456750|gb|AF201456.1|AF201456
Length = 567
Score = 29.1 bits (62), Expect = 2.1
Identities = 20/67 (29%), Positives = 33/67 (49%), Gaps = 3/67 (4%)
Frame = +1
Query: 256 GFFKYPEAELMTRWY--QAAAFQPF-FRAHSHIETKRREPWLXXDGTTVLIRDANRKRYA 426
GF +P E + R+Y + +PF F A S + + EPW D + + RD ++
Sbjct: 12 GFRFHPTDEELVRYYLKRKVCNKPFKFDAISVTDIYKSEPWDLPDKSKLKSRDLEWYFFS 71
Query: 427 LLDXWYT 447
+LD Y+
Sbjct: 72 MLDKKYS 78
>At5g67300.1 68418.m08486 myb family transcription factor contains
PFAM profile: myb DNA binding domain PF00249
Length = 305
Score = 28.7 bits (61), Expect = 2.8
Identities = 16/34 (47%), Positives = 20/34 (58%)
Frame = +2
Query: 179 SFLAASVPMCLSLAIAGNSFCGSDVEDSSSIPKL 280
S A S P+ L ++ S GSDV DSS+IP L
Sbjct: 127 SVSAGSPPVVTGLYMSPGSPTGSDVSDSSTIPIL 160
>At3g10500.1 68416.m01260 no apical meristem (NAM) family protein
similar to to NAC2 (GI:645671) [Arabidopsis thaliana];
contains Pfam PF02365: No apical meristem (NAM) protein
Length = 549
Score = 28.7 bits (61), Expect = 2.8
Identities = 20/66 (30%), Positives = 32/66 (48%), Gaps = 3/66 (4%)
Frame = +1
Query: 256 GFFKYPEAELMTRWY--QAAAFQPF-FRAHSHIETKRREPWLXXDGTTVLIRDANRKRYA 426
GF +P E + R+Y + +PF F A S + + EPW D + + RD ++
Sbjct: 12 GFRFHPTDEELVRYYLKRKICNKPFKFDAISVTDVYKSEPWDLPDKSRLKSRDLEWYFFS 71
Query: 427 LLDXWY 444
+LD Y
Sbjct: 72 MLDKKY 77
>At2g33855.1 68415.m04156 expressed protein
Length = 144
Score = 28.7 bits (61), Expect = 2.8
Identities = 17/39 (43%), Positives = 21/39 (53%)
Frame = -2
Query: 180 DHSAVLSPVHTAEYLWVPANXALVSRKGL*TPSARSSSP 64
D+ V SP H ++ WV VSR GL S RS+SP
Sbjct: 43 DYPPVYSPTHLRQW-WVSQFFKRVSRFGLPDTSWRSNSP 80
>At5g41315.1 68418.m05021 basic helix-loop-helix (bHLH) family
protein contains Pfam profile: PF00010 helix-loop-helix
DNA-binding domain ;annotation temporarily based on
supporting cDNA gi|17224394|gb|AF246291.1|AF246291
Length = 637
Score = 28.3 bits (60), Expect = 3.7
Identities = 12/35 (34%), Positives = 22/35 (62%), Gaps = 1/35 (2%)
Frame = +2
Query: 14 RHVHNEYGL-WNLRATNTGLLDRADGVYRPFLLTR 115
R++ YG+ W++ A+ +G+L+ DG Y + TR
Sbjct: 23 RNIQWSYGIFWSVSASQSGVLEWGDGYYNGDIKTR 57
>At3g01420.1 68416.m00065 pathogen-responsive alpha-dioxygenase,
putative similar to pathogen-inducible alpha-dioxygenase
[Nicotiana attenuata] GI:12539609; contains Pfam profile
PF03098: Animal haem peroxidase
Length = 639
Score = 27.5 bits (58), Expect = 6.4
Identities = 13/29 (44%), Positives = 18/29 (62%), Gaps = 1/29 (3%)
Frame = +2
Query: 14 RHVHNEYGLWNLRATNTGL-LDRADGVYR 97
RH+H +Y L N+ T TG+ D A+ YR
Sbjct: 63 RHLHQQYNLLNVGQTPTGIRFDPANYPYR 91
>At1g63650.2 68414.m07202 basic helix-loop-helix (bHLH) family
protein similar to bHLH transcription factor (JAF13)
GB:AAC39455 [Petunia x hybrida]; contains Pfam profile:
PF00010 Helix-loop-helix DNA-binding domain
Length = 596
Score = 27.5 bits (58), Expect = 6.4
Identities = 12/35 (34%), Positives = 21/35 (60%), Gaps = 1/35 (2%)
Frame = +2
Query: 14 RHVHNEYGL-WNLRATNTGLLDRADGVYRPFLLTR 115
R++ YG+ W++ A+ G+L+ DG Y + TR
Sbjct: 22 RNIQWSYGIFWSVSASQPGVLEWGDGYYNGDIKTR 56
>At1g63650.1 68414.m07201 basic helix-loop-helix (bHLH) family
protein similar to bHLH transcription factor (JAF13)
GB:AAC39455 [Petunia x hybrida]; contains Pfam profile:
PF00010 Helix-loop-helix DNA-binding domain
Length = 596
Score = 27.5 bits (58), Expect = 6.4
Identities = 12/35 (34%), Positives = 21/35 (60%), Gaps = 1/35 (2%)
Frame = +2
Query: 14 RHVHNEYGL-WNLRATNTGLLDRADGVYRPFLLTR 115
R++ YG+ W++ A+ G+L+ DG Y + TR
Sbjct: 22 RNIQWSYGIFWSVSASQPGVLEWGDGYYNGDIKTR 56
>At5g37340.2 68418.m04485 zinc finger (ZPR1-type) family protein
contains similarity to zinc-finger protein ZPR1 (Zinc
finger protein 259) [Mus musculus] SWISS-PROT:Q62384
Length = 498
Score = 27.1 bits (57), Expect = 8.5
Identities = 18/46 (39%), Positives = 22/46 (47%)
Frame = -3
Query: 272 GYLKNPXRQNRRNCSRLLPVTSTSARKPQGTTTPPYCHLSIPQSIS 135
GYL NP + + S P T T+A P GT H +I QS S
Sbjct: 219 GYLTNPSQTGQSERSLGTPSTQTTA-LPHGTIGATAGHRAIAQSNS 263
>At5g37340.1 68418.m04484 zinc finger (ZPR1-type) family protein
contains similarity to zinc-finger protein ZPR1 (Zinc
finger protein 259) [Mus musculus] SWISS-PROT:Q62384
Length = 493
Score = 27.1 bits (57), Expect = 8.5
Identities = 18/46 (39%), Positives = 22/46 (47%)
Frame = -3
Query: 272 GYLKNPXRQNRRNCSRLLPVTSTSARKPQGTTTPPYCHLSIPQSIS 135
GYL NP + + S P T T+A P GT H +I QS S
Sbjct: 219 GYLTNPSQTGQSERSLGTPSTQTTA-LPHGTIGATAGHRAIAQSNS 263
>At3g23480.1 68416.m02958 cyclopropane fatty acid synthase-related
similar to cyclopropane synthase [Sterculia foetida]
GI:21069167
Length = 513
Score = 27.1 bits (57), Expect = 8.5
Identities = 12/30 (40%), Positives = 18/30 (60%)
Frame = -3
Query: 332 ARKNGWNAAAWYHLVMSSASGYLKNPXRQN 243
A+K GW + ++SA+ YLK+ RQN
Sbjct: 129 AKKRGWWTPVFLTASLASATYYLKHVCRQN 158
>At1g17760.1 68414.m02198 suppressor of forked protein family
protein / SUF family protein Contains Pfam PF05843:
Suppressor of forked protein (Suf); identical to
cleavage stimulation factor 77 (GI:21591637)
[Arabidopsis thaliana]; similar to suppressor of forked
protein; Su(f) protein [Drosophila virilis] GI:4092534
Length = 734
Score = 27.1 bits (57), Expect = 8.5
Identities = 14/37 (37%), Positives = 18/37 (48%), Gaps = 2/37 (5%)
Frame = -2
Query: 237 KLFPAIA--SDKHIGTEAARNDHSAVLSPVHTAEYLW 133
K PA+ D H T + H A+L+P H E LW
Sbjct: 127 KSLPALNLNEDLHRKTALRKVYHRAILTPTHHVEQLW 163
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,410,787
Number of Sequences: 28952
Number of extensions: 222808
Number of successful extensions: 620
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 596
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 615
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1062855648
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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