Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060107.seq
(658 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g09210.1 68414.m01028 calreticulin 2 (CRT2) identical to SP|Q... 123 9e-29
At1g56340.1 68414.m06476 calreticulin 1 (CRT1) identical to calr... 121 4e-28
At1g08450.1 68414.m00934 calreticulin 3 (CRT3) identical to simi... 119 2e-27
At5g61790.1 68418.m07754 calnexin 1 (CNX1) identical to calnexin... 67 8e-12
At5g07340.1 68418.m00838 calnexin, putative identical to calnexi... 66 1e-11
At1g08450.2 68414.m00935 calreticulin 3 (CRT3) identical to simi... 48 7e-06
At3g22680.1 68416.m02862 expressed protein 31 0.51
At3g31540.1 68416.m04025 hypothetical protein 31 0.89
At1g43570.1 68414.m05001 hypothetical protein 30 1.6
At1g13960.1 68414.m01641 WRKY family transcription factor simila... 30 1.6
At3g56640.1 68416.m06298 exocyst complex subunit Sec15-like fami... 29 3.6
At1g28170.1 68414.m03458 sulfotransferase family protein similar... 29 3.6
At5g40890.1 68418.m04965 chloride channel protein (CLC-a) identi... 28 4.8
At2g31040.1 68415.m03786 ATP synthase protein I -related contain... 28 4.8
At1g53020.1 68414.m06002 ubiquitin-conjugating enzyme family pro... 28 4.8
>At1g09210.1 68414.m01028 calreticulin 2 (CRT2) identical to
SP|Q38858 Calreticulin 2 precursor {Arabidopsis
thaliana}
Length = 424
Score = 123 bits (297), Expect = 9e-29
Identities = 51/83 (61%), Positives = 63/83 (75%)
Frame = +1
Query: 10 ETPYEIMFGPDICGPGTKKVHVIFSYKGKNHLIKKDIRCKDDVYTHLYTLIVKPDNTYEV 189
+TPY IMFGPDICG TKKVH I +Y NHLIKKD+ C+ D TH+YT I++PD TY +
Sbjct: 128 DTPYSIMFGPDICGYSTKKVHAILTYNEANHLIKKDVPCETDQLTHVYTFILRPDATYSI 187
Query: 190 LIDNEKVESGDLEADWDFLPPKK 258
LIDN + ++G L +DWD LPPKK
Sbjct: 188 LIDNVEKQTGSLYSDWDLLPPKK 210
Score = 64.1 bits (149), Expect = 8e-11
Identities = 34/87 (39%), Positives = 36/87 (41%), Gaps = 1/87 (1%)
Frame = +3
Query: 255 KIKDPEAKKPEDWXXXXXXXXXXXXXXXXXXXXX-HIPDPDATKXXXXXXXXXXXXXXXX 431
KIKDP AKKPEDW IPD D+ K
Sbjct: 210 KIKDPSAKKPEDWDEQEYISDPEDKKPDGYDDIPKEIPDTDSKKPEDWDDEEDGEWTAPT 269
Query: 432 IDNPDYKGVWAPKQIDNPAYKGPWVHP 512
I NP+Y G W PKQI NP YKG W P
Sbjct: 270 IPNPEYMGEWKPKQIKNPNYKGKWEAP 296
>At1g56340.1 68414.m06476 calreticulin 1 (CRT1) identical to
calreticulin (crt1) GI:2052379 [Arabidopsis thaliana]
Length = 425
Score = 121 bits (292), Expect = 4e-28
Identities = 49/83 (59%), Positives = 63/83 (75%)
Frame = +1
Query: 10 ETPYEIMFGPDICGPGTKKVHVIFSYKGKNHLIKKDIRCKDDVYTHLYTLIVKPDNTYEV 189
+TPY IMFGPDICG TKKVH I +Y G NHLIKK++ C+ D TH+YT +++PD TY +
Sbjct: 128 DTPYSIMFGPDICGYSTKKVHAILTYNGTNHLIKKEVPCETDQLTHVYTFVLRPDATYSI 187
Query: 190 LIDNEKVESGDLEADWDFLPPKK 258
LIDN + ++G L +DWD LP KK
Sbjct: 188 LIDNVEKQTGSLYSDWDLLPAKK 210
Score = 66.5 bits (155), Expect = 1e-11
Identities = 35/87 (40%), Positives = 37/87 (42%), Gaps = 1/87 (1%)
Frame = +3
Query: 255 KIKDPEAKKPEDWXXXXXXXXXXXXXXXXXXXXX-HIPDPDATKXXXXXXXXXXXXXXXX 431
KIKDP AKKPEDW IPD DA K
Sbjct: 210 KIKDPSAKKPEDWDDKEYIPDPEDTKPAGYDDIPKEIPDTDAKKPEDWDDEEDGEWTAPT 269
Query: 432 IDNPDYKGVWAPKQIDNPAYKGPWVHP 512
I NP+Y G W PK+I NPAYKG W P
Sbjct: 270 IPNPEYNGEWKPKKIKNPAYKGKWKAP 296
>At1g08450.1 68414.m00934 calreticulin 3 (CRT3) identical to similar
to SP|O04153 Calreticulin 3 precursor {Arabidopsis
thaliana}
Length = 424
Score = 119 bits (286), Expect = 2e-27
Identities = 50/93 (53%), Positives = 69/93 (74%)
Frame = +1
Query: 10 ETPYEIMFGPDICGPGTKKVHVIFSYKGKNHLIKKDIRCKDDVYTHLYTLIVKPDNTYEV 189
+TPY +MFGPDICG TKK+HVI SY+G+N+ IKKD++C+ D H YT I++PD +Y V
Sbjct: 134 DTPYSLMFGPDICGTQTKKLHVIVSYQGQNYPIKKDLQCETDKLNHFYTFILRPDASYSV 193
Query: 190 LIDNEKVESGDLEADWDFLPPKKSRTLKPRNQK 288
L+DN++ E G + DWD LPP+K +K +N K
Sbjct: 194 LVDNKEREFGSMYTDWDILPPRK---IKVKNAK 223
Score = 47.6 bits (108), Expect = 7e-06
Identities = 29/87 (33%), Positives = 32/87 (36%), Gaps = 1/87 (1%)
Frame = +3
Query: 255 KIKDPEAKKPEDWXXXXXXXXXXXXXXXXXXXXXH-IPDPDATKXXXXXXXXXXXXXXXX 431
KIK AKKPEDW IPD A +
Sbjct: 216 KIKVKNAKKPEDWDDREYIDDPNDVKPEGFDSIPREIPDRKAKEPEDWDEEENGLWEPPK 275
Query: 432 IDNPDYKGVWAPKQIDNPAYKGPWVHP 512
I N YKG W K+I NP YKG W +P
Sbjct: 276 IPNSAYKGPWKAKRIKNPNYKGKWKNP 302
Score = 29.9 bits (64), Expect = 1.6
Identities = 11/21 (52%), Positives = 14/21 (66%)
Frame = +3
Query: 432 IDNPDYKGVWAPKQIDNPAYK 494
I NP+YKG W IDNP ++
Sbjct: 290 IKNPNYKGKWKNPWIDNPEFE 310
>At5g61790.1 68418.m07754 calnexin 1 (CNX1) identical to calnexin
homolog 1, Arabidopsis thaliana, EMBL:AT08315
[SP|P29402]
Length = 530
Score = 67.3 bits (157), Expect = 8e-12
Identities = 36/86 (41%), Positives = 48/86 (55%), Gaps = 6/86 (6%)
Frame = +1
Query: 10 ETPYEIMFGPDICGPGTKKVHVIFSYKGKN------HLIKKDIRCKDDVYTHLYTLIVKP 171
E+PY IMFGPD CG GT KVH I +K H +K D +H+YT I+KP
Sbjct: 131 ESPYSIMFGPDKCG-GTNKVHFILKHKNPKSGEYVEHHLKFPPSVPYDKLSHVYTAILKP 189
Query: 172 DNTYEVLIDNEKVESGDLEADWDFLP 249
DN +L+D E+ + +L + DF P
Sbjct: 190 DNEVRILVDGEEKKKANLLSGEDFEP 215
Score = 41.1 bits (92), Expect = 6e-04
Identities = 30/95 (31%), Positives = 32/95 (33%), Gaps = 9/95 (9%)
Frame = +3
Query: 255 KIKDPEAKKPEDWXXXXXXXXXXXXXXXXXXXXXHIP----DPDATKXXXXXXXXXXXXX 422
KI DP A KPEDW P DP+ATK
Sbjct: 239 KIPDPNAVKPEDWDEDAPMEIEDEEAEKPEGWLDDEPEEVDDPEATKPEDWDDEEDGMWE 298
Query: 423 XXXIDNPDYK-----GVWAPKQIDNPAYKGPWVHP 512
IDNP + G W NPAYKG W P
Sbjct: 299 APKIDNPKCEAAPGCGEWKRPMKRNPAYKGKWSSP 333
Score = 39.5 bits (88), Expect = 0.002
Identities = 15/22 (68%), Positives = 16/22 (72%)
Frame = +3
Query: 438 NPDYKGVWAPKQIDNPAYKGPW 503
NP YKG W+ IDNPAYKG W
Sbjct: 323 NPAYKGKWSSPLIDNPAYKGIW 344
Score = 37.5 bits (83), Expect = 0.008
Identities = 22/79 (27%), Positives = 28/79 (35%)
Frame = +3
Query: 255 KIKDPEAKKPEDWXXXXXXXXXXXXXXXXXXXXXHIPDPDATKXXXXXXXXXXXXXXXXI 434
++ DPEA KPEDW + + I
Sbjct: 277 EVDDPEATKPEDWDDEEDGMWEAPKIDNPKCEAAP-GCGEWKRPMKRNPAYKGKWSSPLI 335
Query: 435 DNPDYKGVWAPKQIDNPAY 491
DNP YKG+W P+ I NP Y
Sbjct: 336 DNPAYKGIWKPRDIPNPDY 354
Score = 27.5 bits (58), Expect = 8.3
Identities = 10/12 (83%), Positives = 10/12 (83%)
Frame = +3
Query: 258 IKDPEAKKPEDW 293
I DPE KKPEDW
Sbjct: 223 IPDPEDKKPEDW 234
>At5g07340.1 68418.m00838 calnexin, putative identical to calnexin
homolog 2 from Arabidopsis thaliana [SP|Q38798], strong
similarity to calnexin homolog 1, Arabidopsis thaliana,
EMBL:AT08315 [SP|P29402]; contains Pfam profile PF00262
calreticulin family
Length = 532
Score = 66.5 bits (155), Expect = 1e-11
Identities = 38/101 (37%), Positives = 57/101 (56%), Gaps = 8/101 (7%)
Frame = +1
Query: 10 ETPYEIMFGPDICGPGTKKVHVIFSYKGKN------HLIKKDIRCKDDVYTHLYTLIVKP 171
++PY IMFGPD CG T KVH I +K H +K D+ +H+YT ++K
Sbjct: 133 DSPYSIMFGPDKCG-ATNKVHFILKHKNPKSGEFVEHHLKFPPSVPFDMLSHVYTAVLKS 191
Query: 172 DNTYEVLIDNEKVESGDLEADWDFLPPK-KSRTL-KPRNQK 288
DN +L+D E+ + G+L + DF PP S+T+ P ++K
Sbjct: 192 DNEVRILVDGEEKKKGNLLSAEDFEPPLIPSKTIPDPEDKK 232
Score = 39.5 bits (88), Expect = 0.002
Identities = 15/22 (68%), Positives = 16/22 (72%)
Frame = +3
Query: 438 NPDYKGVWAPKQIDNPAYKGPW 503
NP YKG W+ IDNPAYKG W
Sbjct: 325 NPAYKGKWSSPLIDNPAYKGIW 346
Score = 38.7 bits (86), Expect = 0.003
Identities = 22/79 (27%), Positives = 29/79 (36%)
Frame = +3
Query: 255 KIKDPEAKKPEDWXXXXXXXXXXXXXXXXXXXXXHIPDPDATKXXXXXXXXXXXXXXXXI 434
+++DPEA KPEDW + + I
Sbjct: 279 EVEDPEASKPEDWDDEEDGEWEAPKVSNTKCEAAP-GCGEWKRPMKRNPAYKGKWSSPLI 337
Query: 435 DNPDYKGVWAPKQIDNPAY 491
DNP YKG+W P+ I NP Y
Sbjct: 338 DNPAYKGIWKPRDIPNPDY 356
Score = 32.3 bits (70), Expect = 0.29
Identities = 25/95 (26%), Positives = 30/95 (31%), Gaps = 9/95 (9%)
Frame = +3
Query: 255 KIKDPEAKKPEDWXXXXXXXXXXXXXXXXXXXXXHIP----DPDATKXXXXXXXXXXXXX 422
KI DP A KP+DW P DP+A+K
Sbjct: 241 KIPDPNAVKPDDWDEDAPMEIEDEEAEKPEGWLDDEPVEVEDPEASKPEDWDDEEDGEWE 300
Query: 423 XXXIDNPDYK-----GVWAPKQIDNPAYKGPWVHP 512
+ N + G W NPAYKG W P
Sbjct: 301 APKVSNTKCEAAPGCGEWKRPMKRNPAYKGKWSSP 335
Score = 27.5 bits (58), Expect = 8.3
Identities = 10/12 (83%), Positives = 10/12 (83%)
Frame = +3
Query: 258 IKDPEAKKPEDW 293
I DPE KKPEDW
Sbjct: 225 IPDPEDKKPEDW 236
>At1g08450.2 68414.m00935 calreticulin 3 (CRT3) identical to similar
to SP|O04153 Calreticulin 3 precursor {Arabidopsis
thaliana}
Length = 370
Score = 47.6 bits (108), Expect = 7e-06
Identities = 29/87 (33%), Positives = 32/87 (36%), Gaps = 1/87 (1%)
Frame = +3
Query: 255 KIKDPEAKKPEDWXXXXXXXXXXXXXXXXXXXXXH-IPDPDATKXXXXXXXXXXXXXXXX 431
KIK AKKPEDW IPD A +
Sbjct: 162 KIKVKNAKKPEDWDDREYIDDPNDVKPEGFDSIPREIPDRKAKEPEDWDEEENGLWEPPK 221
Query: 432 IDNPDYKGVWAPKQIDNPAYKGPWVHP 512
I N YKG W K+I NP YKG W +P
Sbjct: 222 IPNSAYKGPWKAKRIKNPNYKGKWKNP 248
Score = 41.5 bits (93), Expect = 5e-04
Identities = 18/39 (46%), Positives = 25/39 (64%)
Frame = +1
Query: 172 DNTYEVLIDNEKVESGDLEADWDFLPPKKSRTLKPRNQK 288
D Y VL+DN++ E G + DWD LPP+K +K +N K
Sbjct: 134 DTPYSVLVDNKEREFGSMYTDWDILPPRK---IKVKNAK 169
Score = 29.9 bits (64), Expect = 1.6
Identities = 11/21 (52%), Positives = 14/21 (66%)
Frame = +3
Query: 432 IDNPDYKGVWAPKQIDNPAYK 494
I NP+YKG W IDNP ++
Sbjct: 236 IKNPNYKGKWKNPWIDNPEFE 256
>At3g22680.1 68416.m02862 expressed protein
Length = 163
Score = 31.5 bits (68), Expect = 0.51
Identities = 13/41 (31%), Positives = 21/41 (51%)
Frame = +3
Query: 504 VHPKSTTQSTLLIQTCTSATKSALSVSILWQSQVRYHSLDD 626
+HP + L++ T S L +++LW+S YHS D
Sbjct: 117 IHPTKAEATIWLVEEIHRLTPSHLHMALLWRSDPMYHSFID 157
>At3g31540.1 68416.m04025 hypothetical protein
Length = 699
Score = 30.7 bits (66), Expect = 0.89
Identities = 17/53 (32%), Positives = 23/53 (43%)
Frame = +1
Query: 94 KNHLIKKDIRCKDDVYTHLYTLIVKPDNTYEVLIDNEKVESGDLEADWDFLPP 252
KN I + CKDD YT + + D E L+ + G L+ FL P
Sbjct: 252 KNMKIIEGFPCKDDAYTDYFFFVALEDAVLEDLVGKVLTKWGILDRPIRFLEP 304
>At1g43570.1 68414.m05001 hypothetical protein
Length = 348
Score = 29.9 bits (64), Expect = 1.6
Identities = 18/56 (32%), Positives = 21/56 (37%)
Frame = +3
Query: 450 KGVWAPKQIDNPAYKGPWVHPKSTTQSTLLIQTCTSATKSALSVSILWQSQVRYHS 617
KGVW PK N WV Q T T A S LW+ ++ Y S
Sbjct: 282 KGVWFPKPHQNLLSLLGWVCTIVFQQGTECKSGTAKLTLLAFSAKTLWKQEITYSS 337
>At1g13960.1 68414.m01641 WRKY family transcription factor similar
to WKRY DNA-binding protein GB:AAD17441
Length = 514
Score = 29.9 bits (64), Expect = 1.6
Identities = 22/85 (25%), Positives = 36/85 (42%), Gaps = 2/85 (2%)
Frame = +1
Query: 280 NQKTGMTSPLFQTPKTRSLRIGTSLNTFQIQMPPNLKTGMMRWTENGSHL*LTTQTTRAF 459
N +G P F+ + L I S + +PP L M+ ++ S L L + ++
Sbjct: 95 NSSSGDVDPRFKQNRPTGLMISQSQSPSMFTVPPGLSPAML--LDSPSFLGLFSPVQGSY 152
Query: 460 G--HQNRLITQLTKAHGSTRNRQPR 528
G HQ L +A + N QP+
Sbjct: 153 GMTHQQALAQVTAQAVQANANMQPQ 177
>At3g56640.1 68416.m06298 exocyst complex subunit Sec15-like family
protein contains Pfam profile PF04091: Exocyst complex
subunit Sec15-like
Length = 789
Score = 28.7 bits (61), Expect = 3.6
Identities = 18/47 (38%), Positives = 25/47 (53%)
Frame = +1
Query: 94 KNHLIKKDIRCKDDVYTHLYTLIVKPDNTYEVLIDNEKVESGDLEAD 234
KN KK+ +D TH IV D VL+D E+++S DL +D
Sbjct: 53 KNVARKKEAEIEDLCKTHYEEFIVAVDELRGVLVDAEELKS-DLASD 98
>At1g28170.1 68414.m03458 sulfotransferase family protein similar to
steroid sulfotransferase 3 GI:3420008 from [Brassica
napus]; contains Pfam profile PF00685: Sulfotransferase
domain
Length = 326
Score = 28.7 bits (61), Expect = 3.6
Identities = 14/27 (51%), Positives = 21/27 (77%)
Frame = +3
Query: 570 ALSVSILWQSQVRYHSLDDFPYLLDDP 650
AL+V++L +S+ + HS DD P LLD+P
Sbjct: 80 ALTVALLERSKQK-HSSDDHPLLLDNP 105
>At5g40890.1 68418.m04965 chloride channel protein (CLC-a) identical
to GI:1742952 (gb|AAC05742.1)
Length = 775
Score = 28.3 bits (60), Expect = 4.8
Identities = 14/33 (42%), Positives = 20/33 (60%), Gaps = 1/33 (3%)
Frame = -3
Query: 383 FGGIWIWNVFRLVPILRLLVFGVWN-SGLVIPV 288
FG + +W F L IL L+ FG+ SGL +P+
Sbjct: 451 FGMVSLWIFFGLYCILGLITFGIATPSGLFLPI 483
>At2g31040.1 68415.m03786 ATP synthase protein I -related contains
weaks similarity to Swiss-Prot:P08443 ATP synthase
protein I [Synechococcus sp.]
Length = 350
Score = 28.3 bits (60), Expect = 4.8
Identities = 10/35 (28%), Positives = 23/35 (65%)
Frame = -3
Query: 350 LVPILRLLVFGVWNSGLVIPVFWFLGFRVLDFLGG 246
LVP++ +++F WN+ +++P + F+ ++ L G
Sbjct: 282 LVPVVLVMIFNRWNA-ILVPEYGFMHLELIPMLVG 315
>At1g53020.1 68414.m06002 ubiquitin-conjugating enzyme family
protein similar to ubiquitin-conjugating enzyme
GB:3319990 from [Mus musculus]; contains Pfam profile
PF00179: Ubiquitin-conjugating enzyme
Length = 1163
Score = 28.3 bits (60), Expect = 4.8
Identities = 13/52 (25%), Positives = 26/52 (50%)
Frame = -3
Query: 518 RFRVDPWAFVSWVINLFWCPNALVVWVVNHRWLPFSVHLIIPVFRFGGIWIW 363
+FR++ +F+ V++ F N + NHR + + I + F G W++
Sbjct: 813 KFRINVTSFMKTVVDEF--VNLGAIREANHRGFLYGISFFITIVAFFGSWVY 862
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,120,738
Number of Sequences: 28952
Number of extensions: 347137
Number of successful extensions: 954
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 890
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 936
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1373722560
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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