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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060106.seq
         (685 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g62120.2 68416.m06980 tRNA synthetase class II (G, H, P and S...   120   7e-28
At3g62120.1 68416.m06979 tRNA synthetase class II (G, H, P and S...   120   7e-28
At5g52520.1 68418.m06516 tRNA synthetase class II (G, H, P and S...    78   5e-15
At5g45990.1 68418.m05656 crooked neck protein, putative / cell c...    27   8.8  
At4g15850.1 68417.m02410 DEAD/DEAH box helicase, putative simila...    27   8.8  

>At3g62120.2 68416.m06980 tRNA synthetase class II (G, H, P and S)
           family protein similar to SP|P07814 Bifunctional
           aminoacyl-tRNA synthetase [Includes: Glutamyl-tRNA
           synthetase (EC 6.1.1.17) (Glutamate--tRNA ligase);
           Prolyl-tRNA synthetase (EC 6.1.1.15) (Proline--tRNA
           ligase)] {Homo sapiens}; contains Pfam profiles PF00587:
           tRNA synthetase class II core domain (G, H, P, S and T),
           PF03129: Anticodon binding domain
          Length = 530

 Score =  120 bits (290), Expect = 7e-28
 Identities = 54/82 (65%), Positives = 63/82 (76%)
 Frame = +3

Query: 9   AEFRKLGVKDGYFPIFVSNAALEREKTHIADFAPEVAWVTHSGXSELAEPIAVRPTSXXV 188
           AE +K+ VK+ YFP+FVS   LE+EK HI  FAPEVAWVT SG S+L  PIA+RPTS  V
Sbjct: 99  AEIKKMKVKNCYFPLFVSPGVLEKEKDHIEGFAPEVAWVTKSGKSDLEVPIAIRPTSETV 158

Query: 189 MYPAYAKWIQSHRDLXLKLNXW 254
           MYP Y+KWI+ HRDL LKLN W
Sbjct: 159 MYPYYSKWIRGHRDLPLKLNQW 180



 Score =  115 bits (277), Expect = 3e-26
 Identities = 51/82 (62%), Positives = 60/82 (73%)
 Frame = +2

Query: 257 NVVRWEFKQPQPFLXXREFLWQEGHTAXXXKXXXXXXXLIXLDLYARVYEELLAIPVIKG 436
           NVVRWEF  P PF+  REFLWQEGHTA   K       L  L+LY R+YEE LA+PV+KG
Sbjct: 182 NVVRWEFSNPTPFIRSREFLWQEGHTAFATKAEADEEVLQILELYRRIYEEYLAVPVVKG 241

Query: 437 RKTEKEKFAGGDYTTTVEAYVP 502
            K+E EKFAGG YTT+VEA++P
Sbjct: 242 MKSENEKFAGGLYTTSVEAFIP 263



 Score = 68.9 bits (161), Expect = 3e-12
 Identities = 32/50 (64%), Positives = 40/50 (80%)
 Frame = +1

Query: 511 RXVQGATSHHLGQNFSKMFEIVYDDAETLEKNYVYQNSWGFTTRTIGVMV 660
           R VQGATSH LGQNF+KMFEI +++ E  E   V+QNSW ++TRTIGVM+
Sbjct: 267 RGVQGATSHCLGQNFAKMFEINFEN-EKAETEMVWQNSWAYSTRTIGVMI 315


>At3g62120.1 68416.m06979 tRNA synthetase class II (G, H, P and S)
           family protein similar to SP|P07814 Bifunctional
           aminoacyl-tRNA synthetase [Includes: Glutamyl-tRNA
           synthetase (EC 6.1.1.17) (Glutamate--tRNA ligase);
           Prolyl-tRNA synthetase (EC 6.1.1.15) (Proline--tRNA
           ligase)] {Homo sapiens}; contains Pfam profiles PF00587:
           tRNA synthetase class II core domain (G, H, P, S and T),
           PF03129: Anticodon binding domain
          Length = 530

 Score =  120 bits (290), Expect = 7e-28
 Identities = 54/82 (65%), Positives = 63/82 (76%)
 Frame = +3

Query: 9   AEFRKLGVKDGYFPIFVSNAALEREKTHIADFAPEVAWVTHSGXSELAEPIAVRPTSXXV 188
           AE +K+ VK+ YFP+FVS   LE+EK HI  FAPEVAWVT SG S+L  PIA+RPTS  V
Sbjct: 99  AEIKKMKVKNCYFPLFVSPGVLEKEKDHIEGFAPEVAWVTKSGKSDLEVPIAIRPTSETV 158

Query: 189 MYPAYAKWIQSHRDLXLKLNXW 254
           MYP Y+KWI+ HRDL LKLN W
Sbjct: 159 MYPYYSKWIRGHRDLPLKLNQW 180



 Score =  115 bits (277), Expect = 3e-26
 Identities = 51/82 (62%), Positives = 60/82 (73%)
 Frame = +2

Query: 257 NVVRWEFKQPQPFLXXREFLWQEGHTAXXXKXXXXXXXLIXLDLYARVYEELLAIPVIKG 436
           NVVRWEF  P PF+  REFLWQEGHTA   K       L  L+LY R+YEE LA+PV+KG
Sbjct: 182 NVVRWEFSNPTPFIRSREFLWQEGHTAFATKAEADEEVLQILELYRRIYEEYLAVPVVKG 241

Query: 437 RKTEKEKFAGGDYTTTVEAYVP 502
            K+E EKFAGG YTT+VEA++P
Sbjct: 242 MKSENEKFAGGLYTTSVEAFIP 263



 Score = 68.9 bits (161), Expect = 3e-12
 Identities = 32/50 (64%), Positives = 40/50 (80%)
 Frame = +1

Query: 511 RXVQGATSHHLGQNFSKMFEIVYDDAETLEKNYVYQNSWGFTTRTIGVMV 660
           R VQGATSH LGQNF+KMFEI +++ E  E   V+QNSW ++TRTIGVM+
Sbjct: 267 RGVQGATSHCLGQNFAKMFEINFEN-EKAETEMVWQNSWAYSTRTIGVMI 315


>At5g52520.1 68418.m06516 tRNA synthetase class II (G, H, P and S)
           family protein similar to SP|P07814 Bifunctional
           aminoacyl-tRNA synthetase [Includes: Glutamyl-tRNA
           synthetase (EC 6.1.1.17) (Glutamate--tRNA ligase);
           Prolyl-tRNA synthetase (EC 6.1.1.15) (Proline--tRNA
           ligase)] {Homo sapiens}; contains Pfam profiles PF00587:
           tRNA synthetase class II core domain (G, H, P, S and T),
           PF03129: Anticodon binding domain
          Length = 543

 Score = 78.2 bits (184), Expect = 5e-15
 Identities = 33/81 (40%), Positives = 52/81 (64%)
 Frame = +3

Query: 12  EFRKLGVKDGYFPIFVSNAALEREKTHIADFAPEVAWVTHSGXSELAEPIAVRPTSXXVM 191
           +F++ G  + YFP F+  + +E+E +H+  F+PE+A VT  G  EL E + VRPTS  ++
Sbjct: 116 KFKETGHSNMYFPQFIPYSFIEKEASHVEGFSPELALVTVGGGKELEEKLVVRPTSETIV 175

Query: 192 YPAYAKWIQSHRDLXLKLNXW 254
              + +WI S+RDL L +N W
Sbjct: 176 NHMFTQWIHSYRDLPLMINQW 196



 Score = 73.3 bits (172), Expect = 1e-13
 Identities = 37/79 (46%), Positives = 46/79 (58%)
 Frame = +2

Query: 257 NVVRWEFKQPQPFLXXREFLWQEGHTAXXXKXXXXXXXLIXLDLYARVYEELLAIPVIKG 436
           NV RWE +  +PF+   EFLWQEGHTA              +++Y R   E  AIPVI G
Sbjct: 198 NVTRWEMRT-KPFIRTLEFLWQEGHTAHATPEEAEKEAKQMIEIYTRFAFEQTAIPVIPG 256

Query: 437 RKTEKEKFAGGDYTTTVEA 493
           RK++ E FAG D T T+EA
Sbjct: 257 RKSKLETFAGADITYTIEA 275



 Score = 52.0 bits (119), Expect = 4e-07
 Identities = 23/53 (43%), Positives = 35/53 (66%)
 Frame = +1

Query: 502 GQRRXVQGATSHHLGQNFSKMFEIVYDDAETLEKNYVYQNSWGFTTRTIGVMV 660
           G R+ +Q  TSH+LGQNFS+ F   + D E  E+ +V+Q SW  +TR +G ++
Sbjct: 278 GDRKALQAGTSHNLGQNFSRAFGTQFAD-ENGERQHVWQTSWAVSTRFVGGII 329


>At5g45990.1 68418.m05656 crooked neck protein, putative / cell
           cycle protein, putative similar to Swiss-Prot:P17886
           crooked neck protein [Drosophila melanogaster]
          Length = 673

 Score = 27.5 bits (58), Expect = 8.8
 Identities = 11/31 (35%), Positives = 15/31 (48%)
 Frame = +3

Query: 201 YAKWIQSHRDLXLKLNXWTTSLDGSSNNRNL 293
           YAKW +S  D     + W  +L+G   N  L
Sbjct: 84  YAKWEESQMDYARARSVWERALEGEYRNHTL 114


>At4g15850.1 68417.m02410 DEAD/DEAH box helicase, putative similar
           to D-E-A-D box protein [Drosophila melanogaster]
           GI:499204; contains Pfam profiles PF00270: DEAD/DEAH box
           helicase, PF00271: Helicase conserved C-terminal domain
          Length = 512

 Score = 27.5 bits (58), Expect = 8.8
 Identities = 14/39 (35%), Positives = 20/39 (51%)
 Frame = +1

Query: 472 LHHHGRSLRTGQRRXVQGATSHHLGQNFSKMFEIVYDDA 588
           +H  GR+ R GQ        S+H  + FSK+ E V  D+
Sbjct: 413 IHRAGRTARAGQAGRCFTLLSNHEVRRFSKLLEKVGSDS 451


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,586,424
Number of Sequences: 28952
Number of extensions: 192473
Number of successful extensions: 476
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 455
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 473
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1447936096
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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