Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060088.seq
(663 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S... 117 8e-27
At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) str... 117 8e-27
At2g43680.2 68415.m05430 calmodulin-binding family protein simil... 32 0.30
At2g43680.1 68415.m05429 calmodulin-binding family protein simil... 32 0.30
At1g57750.1 68414.m06552 cytochrome P450, putative similar to cy... 29 2.1
At5g15130.1 68418.m01773 WRKY family transcription factor contai... 29 3.6
At5g07520.1 68418.m00861 glycine-rich protein (GRP18) Oleosin; g... 28 4.8
At4g39480.1 68417.m05585 cytochrome P450 family protein contains... 28 4.8
At5g17650.1 68418.m02069 glycine/proline-rich protein glycine/pr... 28 6.4
At3g12430.1 68416.m01548 expressed protein ; expression supporte... 28 6.4
At5g55100.2 68418.m06869 SWAP (Suppressor-of-White-APricot)/surp... 27 8.4
At5g55100.1 68418.m06868 SWAP (Suppressor-of-White-APricot)/surp... 27 8.4
At5g01010.1 68418.m00001 expressed protein 27 8.4
At2g05520.1 68415.m00584 glycine-rich protein (GRP) identical to... 27 8.4
At1g80480.1 68414.m09427 PRLI-interacting factor L, putative sim... 27 8.4
At1g76010.1 68414.m08825 expressed protein 27 8.4
At1g58070.1 68414.m06581 expressed protein 27 8.4
>At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S
roibosomal protein L4, Arabidopsis thaliana,
EMBL:CAA79104
Length = 407
Score = 117 bits (281), Expect = 8e-27
Identities = 53/105 (50%), Positives = 61/105 (58%)
Frame = +2
Query: 254 SESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWHXXXXXXXXXXX 433
+ESWGTGRAV+RIPRV GGGTHR+GQ AFGNMCRGGRMFAPTK WRRWH
Sbjct: 70 AESWGTGRAVSRIPRVPGGGTHRAGQAAFGNMCRGGRMFAPTKIWRRWHRRVNVNMKRHA 129
Query: 434 XXXXXXXXXXXXXXQARGHIIEKIPSFPWL*PDKVQEINKXQTGL 568
ARGH IE +P P + D + + K +
Sbjct: 130 IVSAIAATAVPALVMARGHKIENVPEMPLVVSDSAEAVEKTSAAI 174
Score = 71.3 bits (167), Expect = 5e-13
Identities = 36/65 (55%), Positives = 43/65 (66%), Gaps = 2/65 (3%)
Frame = +3
Query: 66 SVARPLVSVYSEKSE--TVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKEA 239
+ ARPLV+V + T Q LP V AP+RPD+VN VH +S NSRQPY VSK+A
Sbjct: 5 AAARPLVTVQGLDGDMSTDQSTTVTLPDVMTAPVRPDIVNFVHAQISNNSRQPYAVSKKA 64
Query: 240 GHQTS 254
GHQTS
Sbjct: 65 GHQTS 69
>At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) strong
similarity to 60S ribosomal protein L1 GB:P49691
Length = 406
Score = 117 bits (281), Expect = 8e-27
Identities = 53/105 (50%), Positives = 61/105 (58%)
Frame = +2
Query: 254 SESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWHXXXXXXXXXXX 433
+ESWGTGRAV+RIPRV GGGTHR+GQ AFGNMCRGGRMFAPTK WRRWH
Sbjct: 69 AESWGTGRAVSRIPRVPGGGTHRAGQAAFGNMCRGGRMFAPTKIWRRWHRRVNVNMKRHA 128
Query: 434 XXXXXXXXXXXXXXQARGHIIEKIPSFPWL*PDKVQEINKXQTGL 568
ARGH IE +P P + D + + K +
Sbjct: 129 IVSAIAATAVPALVMARGHKIENVPEMPLVVSDSAEAVEKTSAAI 173
Score = 73.3 bits (172), Expect = 1e-13
Identities = 36/68 (52%), Positives = 47/68 (69%), Gaps = 2/68 (2%)
Frame = +3
Query: 57 MSLSVARPLVSVYSEKSE--TVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVS 230
M+ + ARPLV++ + + T Q + LP V AP+RPD+VN VH +S NSRQPY VS
Sbjct: 1 MAAAAARPLVTIQTLDGDMSTDQSSTVVLPDVMTAPVRPDIVNFVHAQISNNSRQPYAVS 60
Query: 231 KEAGHQTS 254
K+AGHQTS
Sbjct: 61 KKAGHQTS 68
>At2g43680.2 68415.m05430 calmodulin-binding family protein similar
to SF16 protein [Helianthus annuus] GI:560150; contains
Pfam profile PF00612: IQ calmodulin-binding motif
Length = 669
Score = 32.3 bits (70), Expect = 0.30
Identities = 21/70 (30%), Positives = 29/70 (41%)
Frame = +1
Query: 355 WWTYVRPHEALAALAPSRQPPTAESGLGGSRCCYRRPSARSG*RTHY*KDSQLPLVVARQ 534
WW +V LA+ APS P + L SR C P ++S + H D+ R
Sbjct: 465 WWNWVDRQNPLASPAPSYSQPQRDFRLTPSRLC-PSPLSQSSKQHHIRLDNHFDTSTPRS 523
Query: 535 SPRDQQXPNR 564
S P+R
Sbjct: 524 SRSTFHTPSR 533
>At2g43680.1 68415.m05429 calmodulin-binding family protein similar
to SF16 protein [Helianthus annuus] GI:560150; contains
Pfam profile PF00612: IQ calmodulin-binding motif
Length = 668
Score = 32.3 bits (70), Expect = 0.30
Identities = 21/70 (30%), Positives = 29/70 (41%)
Frame = +1
Query: 355 WWTYVRPHEALAALAPSRQPPTAESGLGGSRCCYRRPSARSG*RTHY*KDSQLPLVVARQ 534
WW +V LA+ APS P + L SR C P ++S + H D+ R
Sbjct: 464 WWNWVDRQNPLASPAPSYSQPQRDFRLTPSRLC-PSPLSQSSKQHHIRLDNHFDTSTPRS 522
Query: 535 SPRDQQXPNR 564
S P+R
Sbjct: 523 SRSTFHTPSR 532
>At1g57750.1 68414.m06552 cytochrome P450, putative similar to
cytochrome P450 GI:4688670 from [Catharanthus roseus]
Length = 497
Score = 29.5 bits (63), Expect = 2.1
Identities = 16/49 (32%), Positives = 29/49 (59%)
Frame = +3
Query: 81 LVSVYSEKSETVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCV 227
LV +++ SE+++ PLPF K+P +PD++ H + NS+ C+
Sbjct: 351 LVYLHAALSESMR-LYPPLPFNHKSPAKPDVLPSGH-KVDANSKIVICI 397
>At5g15130.1 68418.m01773 WRKY family transcription factor contains
Pfam profile: PF03106 WRKY DNA -binding domain; TMV
response-related gene product, Nicotiana tabacum,
EMBL:AB024510
Length = 548
Score = 28.7 bits (61), Expect = 3.6
Identities = 14/42 (33%), Positives = 20/42 (47%)
Frame = -3
Query: 529 GLQPREAGNLFNNVSSSLNERWDAGSSNGCRQGRSPLSEVDA 404
G +P+E ++ N +S E W G G R +P S DA
Sbjct: 159 GGEPKEGLSMENRANSGSEEAWAPGKVTGKRSSPAPASGGDA 200
>At5g07520.1 68418.m00861 glycine-rich protein (GRP18) Oleosin;
glycine-rich protein 18 (GRP18) PMID:11431566;
Length = 228
Score = 28.3 bits (60), Expect = 4.8
Identities = 18/64 (28%), Positives = 28/64 (43%), Gaps = 3/64 (4%)
Frame = +2
Query: 206 LEAALLREQGGWSPNQSESWGTGRAVARIPRVRGGGTHRSGQ---GAFGNMCRGGRMFAP 376
+ AL+R + G P +E + + + ++P G G G+ G FGN GG F
Sbjct: 76 MAVALIRRRMGAKPT-AEGTSSAQPLLKLPVYGGYGGFWGGKKFSGTFGNKPGGGNPFGD 134
Query: 377 TKPW 388
W
Sbjct: 135 ISKW 138
>At4g39480.1 68417.m05585 cytochrome P450 family protein contains
Pfam profile: PF00067 cytochrome P450
Length = 989
Score = 28.3 bits (60), Expect = 4.8
Identities = 12/32 (37%), Positives = 20/32 (62%)
Frame = +3
Query: 132 PLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCV 227
P+PF K+P +PD++ H + NSR +C+
Sbjct: 384 PVPFNHKSPAKPDVLPSGH-KVKANSRILFCL 414
Score = 27.5 bits (58), Expect = 8.4
Identities = 11/32 (34%), Positives = 20/32 (62%)
Frame = +3
Query: 132 PLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCV 227
P+PF K+P +PD++ H + NS+ +C+
Sbjct: 857 PVPFQHKSPTKPDVLPSGH-KVDANSKILFCL 887
>At5g17650.1 68418.m02069 glycine/proline-rich protein
glycine/proline-rich protein GPRP - Arabidopsis
thaliana, EMBL:X84315
Length = 173
Score = 27.9 bits (59), Expect = 6.4
Identities = 10/24 (41%), Positives = 12/24 (50%)
Frame = -2
Query: 359 HHDTCYRRHPDRTYEYHHHGHAEF 288
HH Y H Y Y +HGH +F
Sbjct: 122 HHHGHYGHHHGHGYGYGYHGHGKF 145
>At3g12430.1 68416.m01548 expressed protein ; expression supported
by MPSS
Length = 238
Score = 27.9 bits (59), Expect = 6.4
Identities = 12/40 (30%), Positives = 22/40 (55%), Gaps = 2/40 (5%)
Frame = -1
Query: 135 GAWLHPAPSHSSLNTPTLKVGLPID--SFRYFSEAIPLKY 22
G W+H SH+ ++ L VG+ + Y+S + P++Y
Sbjct: 38 GQWIHDVLSHNRFSSHPLVVGVGVQWTPSSYYSASSPVRY 77
>At5g55100.2 68418.m06869 SWAP (Suppressor-of-White-APricot)/surp
domain-containing protein contains Pfam domain PF01805:
Surp module
Length = 844
Score = 27.5 bits (58), Expect = 8.4
Identities = 12/42 (28%), Positives = 21/42 (50%), Gaps = 1/42 (2%)
Frame = -2
Query: 371 RTYVHHDTCYRRHPDRTY-EYHHHGHAEFGRQHVQYPMIQTG 249
R++ H + +H D + E+HHH H R+H ++ G
Sbjct: 735 RSHHHRSRKHEKHRDSSDDEHHHHRHRSSRRKHEDSSDVEHG 776
>At5g55100.1 68418.m06868 SWAP (Suppressor-of-White-APricot)/surp
domain-containing protein contains Pfam domain PF01805:
Surp module
Length = 843
Score = 27.5 bits (58), Expect = 8.4
Identities = 12/42 (28%), Positives = 21/42 (50%), Gaps = 1/42 (2%)
Frame = -2
Query: 371 RTYVHHDTCYRRHPDRTY-EYHHHGHAEFGRQHVQYPMIQTG 249
R++ H + +H D + E+HHH H R+H ++ G
Sbjct: 735 RSHHHRSRKHEKHRDSSDDEHHHHRHRSSRRKHEDSSDVEHG 776
>At5g01010.1 68418.m00001 expressed protein
Length = 409
Score = 27.5 bits (58), Expect = 8.4
Identities = 16/44 (36%), Positives = 27/44 (61%), Gaps = 1/44 (2%)
Frame = -2
Query: 254 TGLVTSLLAHAVGLPRVLGHRNVNIIDQVR-TDGRLEHEREGLG 126
TG+ +L+ + VG+P+VL ++ I Q+ DG +E +RE G
Sbjct: 204 TGVYKTLVKYLVGVPQVL----LDFIRQINDDDGPMEEQRERYG 243
>At2g05520.1 68415.m00584 glycine-rich protein (GRP) identical to
glycine-rich protein; atGRP (GI:259447) [Arabidopsis
thaliana]
Length = 145
Score = 27.5 bits (58), Expect = 8.4
Identities = 17/44 (38%), Positives = 21/44 (47%)
Frame = +2
Query: 230 QGGWSPNQSESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGG 361
QGG Q G GR R +GGG + G G+ G+ CR G
Sbjct: 79 QGGGGRYQG---GGGRYQGGGGRYQGGGGRQGGGGSGGSYCRHG 119
>At1g80480.1 68414.m09427 PRLI-interacting factor L, putative
similar to PRLI-interacting factor L [Arabidopsis
thaliana] GI:11139268; contains Pfam profile PF02492:
Cobalamin synthesis protein/P47K
Length = 444
Score = 27.5 bits (58), Expect = 8.4
Identities = 9/28 (32%), Positives = 13/28 (46%)
Frame = -2
Query: 359 HHDTCYRRHPDRTYEYHHHGHAEFGRQH 276
HHD + H D +++ HH H H
Sbjct: 311 HHDHDHDHHHDHNHDHDHHHHDGHDHHH 338
>At1g76010.1 68414.m08825 expressed protein
Length = 350
Score = 27.5 bits (58), Expect = 8.4
Identities = 18/43 (41%), Positives = 20/43 (46%)
Frame = +2
Query: 230 QGGWSPNQSESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRG 358
+GG+ Q G GR R R RGGG R G G F N G
Sbjct: 305 RGGYDGPQGRGRGRGRG--RGGRGRGGG--RGGDGGFNNRSDG 343
>At1g58070.1 68414.m06581 expressed protein
Length = 284
Score = 27.5 bits (58), Expect = 8.4
Identities = 15/47 (31%), Positives = 25/47 (53%), Gaps = 1/47 (2%)
Frame = +3
Query: 381 SPGGVGTVASTSDSGERPWRQPLLLPASQRSFRLE-DTLLKRFPASL 518
SP +++ T+ S PW+Q LLP S + + E +LK+ A +
Sbjct: 94 SPIASPSISQTASSSSSPWKQRSLLPPSPQGPKEEIMDVLKKLEAEI 140
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,791,238
Number of Sequences: 28952
Number of extensions: 284299
Number of successful extensions: 956
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 887
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 945
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1393347168
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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