Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP01_F_C09
(911 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g41740.1 68415.m05159 villin 2 (VLN2) nearly identical to vil... 75 9e-14
At3g57410.1 68416.m06391 villin 3 (VLN3) nearly identical to vil... 71 1e-12
At2g29890.1 68415.m03630 villin 1 (VLN1) nearly identical to vil... 66 3e-11
At5g57320.1 68418.m07160 villin, putative similar to villin 2 (V... 46 5e-05
At3g21400.1 68416.m02701 expressed protein 30 1.9
At1g57820.1 68414.m06560 zinc finger (C3HC4-type RING finger) fa... 30 1.9
At2g22540.1 68415.m02673 short vegetative phase protein (SVP) id... 29 5.7
At4g29380.1 68417.m04197 protein kinase family protein / WD-40 r... 28 7.5
At3g57600.1 68416.m06417 AP2 domain-containing transcription fac... 28 7.5
At3g26430.1 68416.m03294 GDSL-motif lipase/hydrolase family prot... 28 9.9
>At2g41740.1 68415.m05159 villin 2 (VLN2) nearly identical to villin
2 (VLN2) [Arabidopsis thaliana] GI:3415115
Length = 976
Score = 74.5 bits (175), Expect = 9e-14
Identities = 51/194 (26%), Positives = 82/194 (42%), Gaps = 1/194 (0%)
Frame = -3
Query: 795 ASMNKGDCFXFSXDNDIYVFVGEKAKNVEKLKAISXANQVRDQDHHGRGKVDIVDKYSSD 616
+S+N D F + IY F G + E+ KA+ ++D+ H G V IVD D
Sbjct: 158 SSLNHDDVFILDTEEKIYQFNGANSNIQERAKALEVVQYLKDKYHEGTCDVAIVDDGKLD 217
Query: 615 VDVQKX-FTAXGSGVKDLVPDESTGGDDQEFERNEASNVIXFEVSDATGKIKVTPLSKPF 439
+ F G + DD + + + ++D K+ P+
Sbjct: 218 TESDSGAFWVLFGGFAPI--GRKVANDDDIVPESTPPKL--YCITDG----KMEPIDGDL 269
Query: 438 KQENLSPQNXYILDTIXGNIYVWIGKQATANEKXQAMTKAQELLNAKNYPSWVQVTRVLQ 259
+ L Y+LD IY+W+G+ +E+ A A+E L ++N P VTRV+Q
Sbjct: 270 SKSMLENTKCYLLDC-GAEIYIWVGRVTQVDERKAASQSAEEFLASENRPKATHVTRVIQ 328
Query: 258 NTEPAAFKQYFFTW 217
E +FK F +W
Sbjct: 329 GYESHSFKSNFDSW 342
>At3g57410.1 68416.m06391 villin 3 (VLN3) nearly identical to villin
3 (VLN3) [Arabidopsis thaliana] GI:3415117
Length = 965
Score = 70.9 bits (166), Expect = 1e-12
Identities = 48/194 (24%), Positives = 83/194 (42%), Gaps = 1/194 (0%)
Frame = -3
Query: 795 ASMNKGDCFXFSXDNDIYVFVGEKAKNVEKLKAISXANQVRDQDHHGRGKVDIVDKYSSD 616
+S+N D F IY F G + E+ KA+ ++D+ H G V IVD D
Sbjct: 160 SSLNHDDVFILDTKEKIYQFNGANSNIQERAKALVVIQYLKDKFHEGTSDVAIVDDGKLD 219
Query: 615 VDVQKX-FTAXGSGVKDLVPDESTGGDDQEFERNEASNVIXFEVSDATGKIKVTPLSKPF 439
+ F G + +D+ + + ++D + LSK
Sbjct: 220 TESDSGEFWVLFGGFAPIA--RKVASEDEIIPETTPPKL--YSIADGQVESIDGDLSKSM 275
Query: 438 KQENLSPQNXYILDTIXGNIYVWIGKQATANEKXQAMTKAQELLNAKNYPSWVQVTRVLQ 259
+ N Y+LD I++W+G+ E+ A+ A++ + ++N P ++TRV+Q
Sbjct: 276 LENN----KCYLLDC-GSEIFIWVGRVTQVEERKTAIQAAEDFVASENRPKATRITRVIQ 330
Query: 258 NTEPAAFKQYFFTW 217
EP +FK F +W
Sbjct: 331 GYEPHSFKSNFDSW 344
>At2g29890.1 68415.m03630 villin 1 (VLN1) nearly identical to villin
1 (VLN1) [Arabidopsis thaliana] GI:3415113
Length = 909
Score = 66.1 bits (154), Expect = 3e-11
Identities = 43/195 (22%), Positives = 87/195 (44%), Gaps = 2/195 (1%)
Frame = -3
Query: 795 ASMNKGDCFXFSXDNDIYVFVGEKAKNVEKLKAISXANQVRDQDHHGRGKVDIVD--KYS 622
+S+N D F + +++F G + EK KA+ ++D H GR +V ++ K+S
Sbjct: 159 SSLNHDDVFILDTASKVFLFAGCNSSTQEKAKAMEVVEYIKDNKHDGRCEVATIEDGKFS 218
Query: 621 SDVDVQKXFTAXGSGVKDLVPDESTGGDDQEFERNEASNVIXFEVSDATGKIKVTPLSKP 442
D D + ++ G +P S+ QE + + + D G + T S
Sbjct: 219 GDSDAGEFWSFFGGYAP--IPKLSS-STTQEQTQTPCAELFWI---DTKGNLHPTGTSS- 271
Query: 441 FKQENLSPQNXYILDTIXGNIYVWIGKQATANEKXQAMTKAQELLNAKNYPSWVQVTRVL 262
++ L Y+LD ++VW+G+ + E+ +++ ++E L + + + +
Sbjct: 272 LDKDMLEKNKCYMLD-CHSEVFVWMGRNTSLTERKTSISSSEEFLRKEGRSTTTSLVLLT 330
Query: 261 QNTEPAAFKQYFFTW 217
+ E A F+ +F W
Sbjct: 331 EGLENARFRSFFNKW 345
Score = 35.9 bits (79), Expect = 0.037
Identities = 26/97 (26%), Positives = 45/97 (46%), Gaps = 4/97 (4%)
Frame = -3
Query: 495 FEVSDATGKIKVTPLSKPFKQENLSPQNXYILDTIXGNIYVWIGKQATANEKXQAMTKAQ 316
F S ++ +KV + F Q++L+ ++ ++LD +YVWIG + K +A+T
Sbjct: 622 FTCSCSSDVLKVKEIYN-FVQDDLTTEDVFLLDC-QSEVYVWIGSNSNIKSKEEALTLGL 679
Query: 315 ELLN----AKNYPSWVQVTRVLQNTEPAAFKQYFFTW 217
+ L + V V + EP F + FF W
Sbjct: 680 KFLEMDILEEGLTMRTPVYVVTEGHEPPFFTR-FFEW 715
Score = 29.9 bits (64), Expect = 2.4
Identities = 31/130 (23%), Positives = 52/130 (40%), Gaps = 2/130 (1%)
Frame = -3
Query: 609 VQKXFTAXGSGVKDLVPDESTGGDDQEFERNEASNVIXFEVS-DATGKIKVTPLSKPFKQ 433
V F G V++L PDE +D N N+ + V D + + +K F
Sbjct: 361 VAALFKQKGYDVEEL-PDE----EDDPLYTNCRDNLKVWRVDGDDVSLLSIPDQTKLFTG 415
Query: 432 ENLSPQNXYIL-DTIXGNIYVWIGKQATANEKXQAMTKAQELLNAKNYPSWVQVTRVLQN 256
+ Q Y + +YVWIG ++ ++ A+T A ++ S + + Q
Sbjct: 416 DCYLVQYKYTYKERTEHLLYVWIGCESIQQDRADAITNASAIVGTTKGES--VLCHIYQG 473
Query: 255 TEPAAFKQYF 226
EP+ F F
Sbjct: 474 NEPSRFFPMF 483
>At5g57320.1 68418.m07160 villin, putative similar to villin 2
(VLN2) [Arabidopsis thaliana] GI:3415115, villin 3
(VLN3) [Arabidopsis thaliana] GI:3415117; contains Pfam
profiles PF00626: Gelsolin repeat, PF02209: Villin
headpiece domain
Length = 962
Score = 45.6 bits (103), Expect = 5e-05
Identities = 30/110 (27%), Positives = 53/110 (48%), Gaps = 4/110 (3%)
Frame = -3
Query: 534 QEFERNEASNVIXFEVSDATGKIKVTPLSKPFKQENLSPQNXYILDTIXGNIYVWIGKQA 355
Q+ +R+ S+ F + +K T + F Q++L ++ +ILD ++VW+G+Q
Sbjct: 614 QKIKRDGESDPHLFSCTYTNESLKATEIFN-FTQDDLMTEDIFILDC-HTEVFVWVGQQV 671
Query: 354 TANEKXQAMTKAQELLN----AKNYPSWVQVTRVLQNTEPAAFKQYFFTW 217
+K QA+ + L +N S + V + EP F + FFTW
Sbjct: 672 DPKKKPQALDIGENFLKHDFLLENLASETPIYIVTEGNEPPFFTR-FFTW 720
>At3g21400.1 68416.m02701 expressed protein
Length = 188
Score = 30.3 bits (65), Expect = 1.9
Identities = 18/41 (43%), Positives = 24/41 (58%), Gaps = 2/41 (4%)
Frame = -3
Query: 717 NVEKLKAISXANQVRDQDHHG-RGKVDIVDKYSSDV-DVQK 601
NV +++ I Q + QDHHG G +I +KY DV VQK
Sbjct: 128 NVAQVRHIMLLFQGKSQDHHGPMGVNEIAEKYRIDVSQVQK 168
>At1g57820.1 68414.m06560 zinc finger (C3HC4-type RING finger)
family protein low similarity to nuclear protein np95
[Mus musculus] GI:4220590; contains Pfam profiles
PF02182: YDG/SRA domain, PF00097: Zinc finger, C3HC4
type (RING finger), PF00628: PHD-finger
Length = 645
Score = 30.3 bits (65), Expect = 1.9
Identities = 18/60 (30%), Positives = 30/60 (50%), Gaps = 3/60 (5%)
Frame = -3
Query: 678 VRDQDHHGRGKVDIVDKYSSDVDVQKXFTAXGSGVKDLVPDESTGGD---DQEFERNEAS 508
+ Q +G V + Y D D + F GSG +DL ++ T + DQ+FE++ A+
Sbjct: 303 IAGQSTYGAQSVALSGGYKDDEDHGEWFLYTGSGGRDLSGNKRTNKEQSFDQKFEKSNAA 362
>At2g22540.1 68415.m02673 short vegetative phase protein (SVP)
identical to cDNA short vegetative phase protein (SVP)
GI:10944319;
Length = 240
Score = 28.7 bits (61), Expect = 5.7
Identities = 41/188 (21%), Positives = 76/188 (40%), Gaps = 6/188 (3%)
Frame = -3
Query: 840 KGSEXSAL-DKSIRXIASMNKGDCFXF--SXDNDIYVFVGEKAKNVEKLKAISXANQVRD 670
K E S L D + I + G F F S ++ ++KN+EKL S Q+ +
Sbjct: 31 KAEELSVLCDADVALIIFSSTGKLFEFCSSSMKEVLERHNLQSKNLEKLDQPSLELQLVE 90
Query: 669 QDHHGRGKVDIVDKYSSDVDVQKXFTAXGSGVKDLVPDESTGGDDQEFERNEASNVIXFE 490
H R +I DK S + + G +++L E S+ I E
Sbjct: 91 NSDHARMSKEIADK-SHRLRQMRGEELQGLDIEELQQLEKALETGLTRVIETKSDKIMSE 149
Query: 489 VSDATGK-IKVTPLSKPFKQE--NLSPQNXYILDTIXGNIYVWIGKQATANEKXQAMTKA 319
+S+ K +++ +K +Q+ L+ +N + I N++ G ++ + +
Sbjct: 150 ISELQKKGMQLMDENKRLRQQGTQLTEENERLGMQICNNVHAHGGAESENAAVYEEGQSS 209
Query: 318 QELLNAKN 295
+ + NA N
Sbjct: 210 ESITNAGN 217
>At4g29380.1 68417.m04197 protein kinase family protein / WD-40 repeat
family protein contains Pfam PF00400: WD domain, G-beta
repeat; contains Pfam PF00069: Protein kinase domain;
contains PF02985: HEAT repeat; similar to adaptor protein
(GI:1817584) [Homo sapiens]; similar to VPS15 protein
(GI:6103009) [Pichia pastoris]
Length = 1494
Score = 28.3 bits (60), Expect = 7.5
Identities = 12/29 (41%), Positives = 18/29 (62%)
Frame = -3
Query: 666 DHHGRGKVDIVDKYSSDVDVQKXFTAXGS 580
DH RG ++V+KYS VD++K G+
Sbjct: 1159 DHISRGLGNVVEKYSGIVDIKKKDVKEGA 1187
>At3g57600.1 68416.m06417 AP2 domain-containing transcription
factor, putative various proteins containing an AP2
transcription factor domain, Arabidopsis thaliana
Length = 277
Score = 28.3 bits (60), Expect = 7.5
Identities = 14/53 (26%), Positives = 25/53 (47%)
Frame = -3
Query: 375 VWIGKQATANEKXQAMTKAQELLNAKNYPSWVQVTRVLQNTEPAAFKQYFFTW 217
+W+G ATA E A +A L + +++ + + +NT P+ F W
Sbjct: 52 LWLGSFATAEEAAMAYDEAA--LKLYGHDAYLNLPHLQRNTRPSLSNSQRFKW 102
>At3g26430.1 68416.m03294 GDSL-motif lipase/hydrolase family protein
similar to early nodulin ENOD8 [Medicago sativa]
GI:304037; contains InterPro Entry IPR001087 Lipolytic
enzyme, G-D-S-L family
Length = 380
Score = 27.9 bits (59), Expect = 9.9
Identities = 12/30 (40%), Positives = 15/30 (50%)
Frame = +1
Query: 319 SFRHGLXFLVCGSLLPDPNVNVAXYGVQNI 408
+F HG F GS + PN +A GV I
Sbjct: 97 NFSHGANFATAGSTVRPPNATIAQSGVSPI 126
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,708,447
Number of Sequences: 28952
Number of extensions: 248605
Number of successful extensions: 681
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 657
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 679
length of database: 12,070,560
effective HSP length: 81
effective length of database: 9,725,448
effective search space used: 2159049456
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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