Silkworm BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BmNP01_FL5_A14
         (868 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g09360.1 68417.m01545 disease resistance protein (NBS-LRR cla...    29   3.0  
At5g40580.2 68418.m04925 20S proteasome beta subunit B (PBB2) (P...    28   7.0  
At5g40580.1 68418.m04924 20S proteasome beta subunit B (PBB2) (P...    28   7.0  
At3g55220.1 68416.m06133 splicing factor, putative contains CPSF...    28   7.0  
At3g55200.1 68416.m06131 splicing factor, putative contains CPSF...    28   7.0  
At3g27430.2 68416.m03429 20S proteasome beta subunit B (PBB1) id...    28   9.3  
At3g27430.1 68416.m03428 20S proteasome beta subunit B (PBB1) id...    28   9.3  

>At4g09360.1 68417.m01545 disease resistance protein (NBS-LRR
           class), putative domain signature NBS-LRR exists,
           suggestive of a disease resistance protein.
          Length = 853

 Score = 29.5 bits (63), Expect = 3.0
 Identities = 23/72 (31%), Positives = 30/72 (41%)
 Frame = +2

Query: 29  HKMSTTYIILLPFLLTCAIASAAECENATSLSLMIDSLLATYDRESPPDSKIVVNLTLHL 208
           H  ST   +  P LL+ A      CE +  L     S    YD     D  I +NLT +L
Sbjct: 718 HGTSTKISLFTPTLLSFAACILISCERSFYLQFPAFS----YDWNRKDDEVISINLTPNL 773

Query: 209 RHANIRESESTV 244
             ++  E E TV
Sbjct: 774 NLSSEIEEEETV 785


>At5g40580.2 68418.m04925 20S proteasome beta subunit B (PBB2)
           (PRCFC) identical to 20S proteasome beta subunit PBB2
           [Arabidopsis thaliana] GI:3421104, cDNA proteasome
           subunit prcfc GI:2511575
          Length = 274

 Score = 28.3 bits (60), Expect = 7.0
 Identities = 16/53 (30%), Positives = 27/53 (50%)
 Frame = +2

Query: 56  LLPFLLTCAIASAAECENATSLSLMIDSLLATYDRESPPDSKIVVNLTLHLRH 214
           + P +  C   +AA+ E  T    M+ S L  +  ++  DS++V  LTL  +H
Sbjct: 76  MAPNIYCCGAGTAADTEAVTD---MVSSQLRLHRYQTGRDSRVVTALTLLKKH 125


>At5g40580.1 68418.m04924 20S proteasome beta subunit B (PBB2)
           (PRCFC) identical to 20S proteasome beta subunit PBB2
           [Arabidopsis thaliana] GI:3421104, cDNA proteasome
           subunit prcfc GI:2511575
          Length = 274

 Score = 28.3 bits (60), Expect = 7.0
 Identities = 16/53 (30%), Positives = 27/53 (50%)
 Frame = +2

Query: 56  LLPFLLTCAIASAAECENATSLSLMIDSLLATYDRESPPDSKIVVNLTLHLRH 214
           + P +  C   +AA+ E  T    M+ S L  +  ++  DS++V  LTL  +H
Sbjct: 76  MAPNIYCCGAGTAADTEAVTD---MVSSQLRLHRYQTGRDSRVVTALTLLKKH 125


>At3g55220.1 68416.m06133 splicing factor, putative contains CPSF A
           subunit region (PF03178); contains weak WD-40 repeat
           (PF00400); similar to Splicing factor 3B subunit 3
           (SF3b130)/spliceosomal protein/Splicing factor 3B
           subunit 3 (SAP 130)(KIAA0017)(SP:Q15393) Homo sapiens,
           EMBL:HSAJ1443_1
          Length = 1214

 Score = 28.3 bits (60), Expect = 7.0
 Identities = 13/30 (43%), Positives = 18/30 (60%)
 Frame = -3

Query: 413 GRGERSPRRWLQPRLAVPRQASTSVPRTPA 324
           GRG RS  R L+P LA+   A + +P  P+
Sbjct: 426 GRGPRSSLRILRPGLAITEMAVSQLPGQPS 455


>At3g55200.1 68416.m06131 splicing factor, putative contains CPSF A
           subunit region (PF03178); contains weak WD-40 repeat
           (PF00400); similar to Splicing factor 3B subunit 3
           (SF3b130)/spliceosomal protein/Splicing factor 3B
           subunit 3 (SAP 130)(KIAA0017)(SP:Q15393) Homo sapiens,
           EMBL:HSAJ1443_1
          Length = 1214

 Score = 28.3 bits (60), Expect = 7.0
 Identities = 13/30 (43%), Positives = 18/30 (60%)
 Frame = -3

Query: 413 GRGERSPRRWLQPRLAVPRQASTSVPRTPA 324
           GRG RS  R L+P LA+   A + +P  P+
Sbjct: 426 GRGPRSSLRILRPGLAITEMAVSQLPGQPS 455


>At3g27430.2 68416.m03429 20S proteasome beta subunit B (PBB1)
           identical to 20S proteasome beta subunit PBB1 (PBB1)
           GB:AAC32066 [Arabidopsis thaliana] (Genetics 149 (2),
           677-692 (1998)); contains Pfam profile: PF00227
           proteasome A-type and B-type;
          Length = 273

 Score = 27.9 bits (59), Expect = 9.3
 Identities = 15/53 (28%), Positives = 27/53 (50%)
 Frame = +2

Query: 56  LLPFLLTCAIASAAECENATSLSLMIDSLLATYDRESPPDSKIVVNLTLHLRH 214
           + P +  C   +AA+ E  T    M+ S L  +  ++  DS+++  LTL  +H
Sbjct: 76  MAPNIYCCGAGTAADTEAVTD---MVSSQLRLHRYQTGRDSRVITALTLLKKH 125


>At3g27430.1 68416.m03428 20S proteasome beta subunit B (PBB1)
           identical to 20S proteasome beta subunit PBB1 (PBB1)
           GB:AAC32066 [Arabidopsis thaliana] (Genetics 149 (2),
           677-692 (1998)); contains Pfam profile: PF00227
           proteasome A-type and B-type;
          Length = 267

 Score = 27.9 bits (59), Expect = 9.3
 Identities = 15/53 (28%), Positives = 27/53 (50%)
 Frame = +2

Query: 56  LLPFLLTCAIASAAECENATSLSLMIDSLLATYDRESPPDSKIVVNLTLHLRH 214
           + P +  C   +AA+ E  T    M+ S L  +  ++  DS+++  LTL  +H
Sbjct: 76  MAPNIYCCGAGTAADTEAVTD---MVSSQLRLHRYQTGRDSRVITALTLLKKH 125


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,861,228
Number of Sequences: 28952
Number of extensions: 388506
Number of successful extensions: 1073
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 1008
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1069
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 2028915200
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)