Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030623sawa_E11_e85_09.seq
(1515 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g18110.1 68415.m02105 elongation factor 1-beta, putative / EF... 138 9e-33
At5g19510.1 68418.m02324 elongation factor 1B alpha-subunit 2 (e... 135 6e-32
At1g30230.1 68414.m03698 elongation factor 1-beta / EF-1-beta id... 134 2e-31
At5g12110.1 68418.m01422 elongation factor 1B alpha-subunit 1 (e... 132 8e-31
At2g40660.1 68415.m05017 tRNA-binding region domain-containing p... 32 0.86
At2g34400.1 68415.m04215 pentatricopeptide (PPR) repeat-containi... 31 1.5
At5g08415.1 68418.m00991 lipoic acid synthase family protein sim... 30 4.6
At3g46390.1 68416.m05029 hypothetical protein 29 8.0
>At2g18110.1 68415.m02105 elongation factor 1-beta, putative /
EF-1-beta, putative nearly identical to eEF-1beta
[Arabidopsis thaliana] GI:398606
Length = 231
Score = 138 bits (334), Expect = 9e-33
Identities = 82/213 (38%), Positives = 110/213 (51%), Gaps = 11/213 (5%)
Frame = +2
Query: 125 ALGDVKTAQGLNELNQYLAERSYVSGYTPSQADIKVFEQVGKVPAASLPHVLRWYSHI-- 298
A ++ + GL +L+++L RSY++GY S+ DI VF + K P + +V RW++HI
Sbjct: 3 AFPNLNSGSGLKKLDEHLLTRSYITGYQASKDDITVFTALSKPPTSEFVNVSRWFNHIDA 62
Query: 299 ---ASYTPAERK-TWSEGVSXXXXXXXXXXXXXXSNXXXXXXXX-----LFGSGXXXXXX 451
S AE EG S S LFG
Sbjct: 63 LLRISGVSAEGSGVIVEGSSPITEEAVATPPAADSKDTAAEEEDDDDVDLFGE-ETEEEK 121
Query: 452 XXXXXXXXXLKAYADKKSKKPALIAKSSIILDVKPWDDETDMKEMENQVRTIEMDGLLWG 631
+KA KK KSS+++D+KPWDDETDMK++E VR+I+M+GL WG
Sbjct: 122 KAAEERAASVKASTKKKES-----GKSSVLMDIKPWDDETDMKKLEEAVRSIQMEGLFWG 176
Query: 632 ASKLVPVGYGINKLQIMCVIEDDKVSVDLLTEK 730
ASKLVPVGYGI KL IMC I DD VS+D + E+
Sbjct: 177 ASKLVPVGYGIKKLHIMCTIVDDLVSIDTMIEE 209
>At5g19510.1 68418.m02324 elongation factor 1B alpha-subunit 2
(eEF1Balpha2) identical to elongation factor 1B
alpha-subunit [Arabidopsis thaliana] GI:6686821
Length = 224
Score = 135 bits (327), Expect = 6e-32
Identities = 77/207 (37%), Positives = 104/207 (50%), Gaps = 3/207 (1%)
Frame = +2
Query: 122 MALGDVKTAQGLNELNQYLAERSYVSGYTPSQADIKVFEQVGKVPAASLPHVLRWYSHIA 301
+ D+ T +G+ + ++LA ++Y+SG S D+KV+ V P+ + P+ +WY +A
Sbjct: 3 VTFSDLHTEEGVKSVEEHLAGKTYISGDQLSVDDVKVYAAVPVKPSDAFPNASKWYESVA 62
Query: 302 SYTPAERKTWSEGVSXXXXXXXXXXXXXXSNXXXXXXXX---LFGSGXXXXXXXXXXXXX 472
S + GV + LFG
Sbjct: 63 SQLAKSFPGKAVGVQFGGSAAAAPAVEAEAPAAAADDDDDMDLFGDETEEEKKAAEE--- 119
Query: 473 XXLKAYADKKSKKPALIAKSSIILDVKPWDDETDMKEMENQVRTIEMDGLLWGASKLVPV 652
+ A K +KKP KSS+++DVKPWDDETDMK++E VR +EM GL WGASKLVPV
Sbjct: 120 ---REAAKKDTKKPKESGKSSVLMDVKPWDDETDMKKLEEAVRGVEMPGLFWGASKLVPV 176
Query: 653 GYGINKLQIMCVIEDDKVSVDLLTEKF 733
GYGI KL IM I DD VS D L E F
Sbjct: 177 GYGIKKLTIMFTIVDDLVSPDNLIEDF 203
>At1g30230.1 68414.m03698 elongation factor 1-beta / EF-1-beta
identical to SP|P48006 Elongation factor 1-beta
(EF-1-beta) {Arabidopsis thaliana}
Length = 231
Score = 134 bits (323), Expect = 2e-31
Identities = 77/213 (36%), Positives = 109/213 (51%), Gaps = 11/213 (5%)
Frame = +2
Query: 125 ALGDVKTAQGLNELNQYLAERSYVSGYTPSQADIKVFEQVGKVPAASLPHVLRWYSH--- 295
A ++ + GL +L+++L RSY++GY S+ DI VF + K P + + RWY+H
Sbjct: 3 AFPNLNSDAGLKKLDEHLLTRSYITGYQASKDDITVFAALAKPPTSQYVNASRWYNHIDA 62
Query: 296 ---IASYTPAERKTWSEGVSXXXXXXXXXXXXXXSNXXXXXXXX-----LFGSGXXXXXX 451
I+ + EG + S LFG
Sbjct: 63 LLRISGVSAEGSGVIVEGSAPITEEAVATPPAADSKDAAADEEDDDDVDLFGE-ETEEEK 121
Query: 452 XXXXXXXXXLKAYADKKSKKPALIAKSSIILDVKPWDDETDMKEMENQVRTIEMDGLLWG 631
+KA KK KSS+++D+KPWDDETDMK++E V++I+M+GL WG
Sbjct: 122 KAAEERAASVKASTKKKES-----GKSSVLIDIKPWDDETDMKKLEEAVKSIQMEGLFWG 176
Query: 632 ASKLVPVGYGINKLQIMCVIEDDKVSVDLLTEK 730
ASKLVPVGYGI KLQI+C I DD VS+D + E+
Sbjct: 177 ASKLVPVGYGIKKLQILCTIVDDLVSIDTMIEE 209
>At5g12110.1 68418.m01422 elongation factor 1B alpha-subunit 1
(eEF1Balpha1) identical to elongation factor 1B
alpha-subunit [Arabidopsis thaliana] GI:6686819
Length = 228
Score = 132 bits (318), Expect = 8e-31
Identities = 75/203 (36%), Positives = 103/203 (50%), Gaps = 1/203 (0%)
Frame = +2
Query: 122 MALGDVKTAQGLNELNQYLAERSYVSGYTPSQADIKVFEQVGKVPAASLPHVLRWYSHIA 301
+ D+ T +GL L ++LA ++Y+SG S D+KV+ V + P P+ +WY +A
Sbjct: 3 VTFSDLHTERGLKTLEEHLAGKTYISGDQLSVDDVKVYAAVLENPGDGFPNASKWYDSVA 62
Query: 302 SYTPAERKTWSEGVSXXXXXXXXXXXXXXSNXXXXXXXXLFGSGXXXXXXXXXXXXXXXL 481
S+ ++GV +
Sbjct: 63 SHLAKSFPGKADGVRVGGGVAPPSEAHPHTEEPAADGDGDDDDDIDLFADETEDEKKAAE 122
Query: 482 KAYADKK-SKKPALIAKSSIILDVKPWDDETDMKEMENQVRTIEMDGLLWGASKLVPVGY 658
+ A KK +KK KSS++L+VKPWDDETDMK++E VR+++M GL WGASKLVPVGY
Sbjct: 123 EREAAKKDTKKTKESGKSSVLLEVKPWDDETDMKKLEEAVRSVQMPGLTWGASKLVPVGY 182
Query: 659 GINKLQIMCVIEDDKVSVDLLTE 727
GI KL IM I DD VSVD L E
Sbjct: 183 GIKKLTIMMTIVDDLVSVDNLIE 205
>At2g40660.1 68415.m05017 tRNA-binding region domain-containing
protein similar to SP|Q12904 Multisynthetase complex
auxiliary component p43 [Contains: Endothelial-monocyte
activating polypeptide II (EMAP-II) (Small inducible
cytokine subfamily E member 1)] {Homo sapiens}; contains
Pfam profile PF01588: Putative tRNA binding domain
Length = 389
Score = 32.3 bits (70), Expect = 0.86
Identities = 22/57 (38%), Positives = 32/57 (56%), Gaps = 9/57 (15%)
Frame = +2
Query: 155 LNELNQYLAERSYV--SGYTPSQADIKVFEQV-GKVPAAS------LPHVLRWYSHI 298
L +LN LA +S + +G TPS AD+ VF + V S +PHV+RW ++I
Sbjct: 76 LEKLNLELATKSVLLGNGLTPSAADVAVFSALHSSVLGLSDSDKEKVPHVIRWVNYI 132
>At2g34400.1 68415.m04215 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 617
Score = 31.5 bits (68), Expect = 1.5
Identities = 16/78 (20%), Positives = 33/78 (42%)
Frame = +2
Query: 521 IAKSSIILDVKPWDDETDMKEMENQVRTIEMDGLLWGASKLVPVGYGINKLQIMCVIEDD 700
+ S+++ D+K WD+ M+ + ++ G W + + + + C ED
Sbjct: 535 VISSNVLADMKMWDESAKMRALMRDRGVVKTPGCSWIEIEGELMEFLAGSDYLQCGREDS 594
Query: 701 KVSVDLLTEKFKNLKISY 754
DLL E+ K + +
Sbjct: 595 GSLFDLLVEEMKRERYEF 612
>At5g08415.1 68418.m00991 lipoic acid synthase family protein
similar to lipoic acid synthase from Arabidopsis
thaliana [gi:3928758], from Mus musculus [gi:14669826]
Pfam profile PF04055: radical SAM domain protein
Length = 394
Score = 29.9 bits (64), Expect = 4.6
Identities = 17/44 (38%), Positives = 26/44 (59%)
Frame = +2
Query: 500 KSKKPALIAKSSIILDVKPWDDETDMKEMENQVRTIEMDGLLWG 631
K KP +I K+SI+L + D+E +KE +R I++D L G
Sbjct: 287 KISKPGMITKTSIMLGLGETDEE--LKEAMADLRAIDVDILTLG 328
>At3g46390.1 68416.m05029 hypothetical protein
Length = 69
Score = 29.1 bits (62), Expect = 8.0
Identities = 19/69 (27%), Positives = 33/69 (47%), Gaps = 2/69 (2%)
Frame = +2
Query: 479 LKAYADKKSKKPALIAKSSIILDVKPWDDETDMKEMENQVRTIEMDGLLWGA--SKLVPV 652
+K Y+ K K+ + +I +D+K +M + E + I M G++W S L+ V
Sbjct: 1 MKRYSTKHFKRILIANNVNIDIDLKTGIVAKNMAKKEKIEQVISMGGIMWEGLNSSLIKV 60
Query: 653 GYGINKLQI 679
+ K QI
Sbjct: 61 DEALLKQQI 69
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 22,907,387
Number of Sequences: 28952
Number of extensions: 388031
Number of successful extensions: 804
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 783
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 803
length of database: 12,070,560
effective HSP length: 84
effective length of database: 9,638,592
effective search space used: 4048208640
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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