BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30036
(699 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC13G6.02c |rps101|rps1-1, rps3a-1|40S ribosomal protein S3a|S... 116 4e-27
SPAC22H12.04c |rps102|rps1-2, rps3a-2|40S ribosomal protein S3a|... 113 3e-26
SPBC32H8.13c |mok12||alpha-1,3-glucan synthase Mok12|Schizosacch... 27 2.6
SPAC343.04c |gnr1||heterotrimeric G protein beta subunit Gnr1|Sc... 27 3.4
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 27 3.4
SPBC337.13c |gtr1||Gtr1/RagA G protein Gtr1|Schizosaccharomyces ... 26 6.0
SPBC12C2.02c |ste20|ste16|sterility protein Ste20|Schizosaccharo... 26 6.0
SPAC19E9.02 |fin1||serine/threonine protein kinase Fin1|Schizosa... 26 6.0
SPBC1921.05 |ape2||aminopeptidase Ape2|Schizosaccharomyces pombe... 25 7.9
SPAC7D4.06c |alg3||dolichol-P-Man dependent alpha|Schizosaccharo... 25 7.9
SPAC664.08c |||traub transciption factor family protein |Schizos... 25 7.9
>SPAC13G6.02c |rps101|rps1-1, rps3a-1|40S ribosomal protein
S3a|Schizosaccharomyces pombe|chr 1|||Manual
Length = 252
Score = 116 bits (278), Expect = 4e-27
Identities = 53/86 (61%), Positives = 66/86 (76%)
Frame = +2
Query: 2 KKGVKKKIVDPFTRKDWYDVKAPSMFSKRQVGTTLVNRTQGTKIASEGLKGRVFEVSLAD 181
KKG+KK++VDPF+RKDWYD+KAP+ F + VG TLVNRT G K A++ LKGR+ EVSLAD
Sbjct: 13 KKGIKKRVVDPFSRKDWYDIKAPAFFEVKNVGKTLVNRTAGLKNANDSLKGRILEVSLAD 72
Query: 182 LQADTDAERSFRKFRLIAEYVQGRMC 259
LQ D E SFRK +L E +QG+ C
Sbjct: 73 LQ--KDEEHSFRKVKLRVEDIQGKSC 96
Score = 97.5 bits (232), Expect = 2e-21
Identities = 46/103 (44%), Positives = 66/103 (64%)
Frame = +1
Query: 259 LCNFHGMDLTTDKLRWMVKKWQTLIEANIDVKTTDGYVLRVFCIGFTNKDSLSQRKTCYA 438
L +F+G D+T+DKLR +V+KWQ+ IEAN +KTTDGY+ R+F IGFT++ +KT YA
Sbjct: 97 LTSFNGFDMTSDKLRSLVRKWQSTIEANQTIKTTDGYLCRIFVIGFTSRRVNQVKKTTYA 156
Query: 439 QHTQVRAIRKKMCEIITRDVTNSDSGRW*TS*FPDSIAKDIEK 567
Q +Q+RAI +KM ++I P+ I + IEK
Sbjct: 157 QSSQIRAIHQKMFQVIQNQANGCSMKELVQKLIPEVIGRAIEK 199
Score = 25.8 bits (54), Expect = 6.0
Identities = 13/26 (50%), Positives = 19/26 (73%)
Frame = +3
Query: 585 YPLRDVWHPKVKVLKRAPFSEISKLI 662
YPL++V+ KVK+LK AP + KL+
Sbjct: 205 YPLQNVFVRKVKILK-APKHDAQKLL 229
>SPAC22H12.04c |rps102|rps1-2, rps3a-2|40S ribosomal protein
S3a|Schizosaccharomyces pombe|chr 1|||Manual
Length = 252
Score = 113 bits (271), Expect = 3e-26
Identities = 51/86 (59%), Positives = 66/86 (76%)
Frame = +2
Query: 2 KKGVKKKIVDPFTRKDWYDVKAPSMFSKRQVGTTLVNRTQGTKIASEGLKGRVFEVSLAD 181
KKG+KK++VDPF+RK+WYD+KAP+ F + VG TLVNRT G K A++ LKGR+ EVSLAD
Sbjct: 13 KKGIKKRVVDPFSRKEWYDIKAPAFFEVKNVGKTLVNRTAGLKNANDSLKGRILEVSLAD 72
Query: 182 LQADTDAERSFRKFRLIAEYVQGRMC 259
LQ D E +FRK +L E +QG+ C
Sbjct: 73 LQ--KDEEHAFRKVKLRVEDIQGKSC 96
Score = 99 bits (238), Expect = 3e-22
Identities = 46/103 (44%), Positives = 70/103 (67%)
Frame = +1
Query: 259 LCNFHGMDLTTDKLRWMVKKWQTLIEANIDVKTTDGYVLRVFCIGFTNKDSLSQRKTCYA 438
L +F+G+ +T+DKLR +V+KWQT IEA+ +KTTDGY+ RVF IGFT + + +KT YA
Sbjct: 97 LTSFNGLSITSDKLRSLVRKWQTTIEADQTIKTTDGYLCRVFVIGFTRRRANQVKKTTYA 156
Query: 439 QHTQVRAIRKKMCEIITRDVTNSDSGRW*TS*FPDSIAKDIEK 567
Q +Q+RAIR+KM ++I ++ P+ I ++IE+
Sbjct: 157 QSSQIRAIRQKMFQVIQNQTSSCSMRELVQKLIPEVIGREIER 199
>SPBC32H8.13c |mok12||alpha-1,3-glucan synthase
Mok12|Schizosaccharomyces pombe|chr 2|||Manual
Length = 2352
Score = 27.1 bits (57), Expect = 2.6
Identities = 10/22 (45%), Positives = 17/22 (77%)
Frame = -2
Query: 569 PFSMSLAMESGNQLVHHLPESE 504
PFS+ L +++ ++L+ LPESE
Sbjct: 1146 PFSVHLPLDNSSELLKQLPESE 1167
>SPAC343.04c |gnr1||heterotrimeric G protein beta subunit
Gnr1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 507
Score = 26.6 bits (56), Expect = 3.4
Identities = 14/44 (31%), Positives = 23/44 (52%)
Frame = +1
Query: 238 ICARTYVLCNFHGMDLTTDKLRWMVKKWQTLIEANIDVKTTDGY 369
IC YVL NF D +K R + ++ ++N ++ T +GY
Sbjct: 95 ICEAIYVLQNFESTDFNKEKERLVSIILESNNKSNNELITKNGY 138
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 26.6 bits (56), Expect = 3.4
Identities = 14/32 (43%), Positives = 20/32 (62%), Gaps = 1/32 (3%)
Frame = -1
Query: 699 PPCLHRLR-RESSQSTSRSPKTGPSSTLSLSD 607
PP ++ R SS STS + + G ST+SLS+
Sbjct: 10 PPTSRSIQSRNSSYSTSSNERIGTPSTISLSE 41
>SPBC337.13c |gtr1||Gtr1/RagA G protein Gtr1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 308
Score = 25.8 bits (54), Expect = 6.0
Identities = 14/37 (37%), Positives = 22/37 (59%), Gaps = 2/37 (5%)
Frame = +1
Query: 256 VLCNFHGMDLTTDKLRWMV--KKWQTLIEANIDVKTT 360
V C H MDL + LR +V ++ L+E + D++TT
Sbjct: 117 VFCLIHKMDLVQEDLRDLVFEERKAILLETSKDLETT 153
>SPBC12C2.02c |ste20|ste16|sterility protein Ste20|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1309
Score = 25.8 bits (54), Expect = 6.0
Identities = 12/33 (36%), Positives = 18/33 (54%), Gaps = 2/33 (6%)
Frame = +3
Query: 384 LHWFHQ*GLLEPTQDVLRPAHSGQ--SNQKENV 476
LHW+ L+ +Q + P H+GQ S + NV
Sbjct: 1162 LHWYSLMSLMGTSQGICIPRHAGQVLSTPRRNV 1194
>SPAC19E9.02 |fin1||serine/threonine protein kinase
Fin1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 722
Score = 25.8 bits (54), Expect = 6.0
Identities = 12/42 (28%), Positives = 20/42 (47%)
Frame = -1
Query: 279 HAVEVAEHIRPCTYSAINLNLRKDLSASVSACRSARETSKTL 154
H +EV +RP TY + + D+ + + + R E S L
Sbjct: 260 HCLEVNSDLRPTTYQLLRSPILSDIRSKLESERVVLEQSDLL 301
>SPBC1921.05 |ape2||aminopeptidase Ape2|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 882
Score = 25.4 bits (53), Expect = 7.9
Identities = 12/46 (26%), Positives = 21/46 (45%)
Frame = +1
Query: 301 RWMVKKWQTLIEANIDVKTTDGYVLRVFCIGFTNKDSLSQRKTCYA 438
++ W L+ T GYV+R GFT+ ++ + K +A
Sbjct: 795 KFATSNWDKLLSRLPVAGTMRGYVVRFVTSGFTHASAIDKIKEFFA 840
>SPAC7D4.06c |alg3||dolichol-P-Man dependent
alpha|Schizosaccharomyces pombe|chr 1|||Manual
Length = 406
Score = 25.4 bits (53), Expect = 7.9
Identities = 15/64 (23%), Positives = 29/64 (45%), Gaps = 4/64 (6%)
Frame = -2
Query: 512 ESELVTSRVMISHIFFLIALT*VCW----A*HVLRWLKESLLVKPMQKTRRTYPSVVFTS 345
ES ++ ++ H+ L+A T W + L +KP+ K + P+ +FT+
Sbjct: 263 ESTSFSTSILFLHVALLVAFTCKHWNKLSRATPFAMVNSMLTLKPLPKLQLATPNFIFTA 322
Query: 344 MLAS 333
+ S
Sbjct: 323 LATS 326
>SPAC664.08c |||traub transciption factor family protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 452
Score = 25.4 bits (53), Expect = 7.9
Identities = 13/34 (38%), Positives = 18/34 (52%), Gaps = 1/34 (2%)
Frame = +1
Query: 280 DLTTD-KLRWMVKKWQTLIEANIDVKTTDGYVLR 378
D T D +RWM K Q + N+D K + G +R
Sbjct: 351 DSTRDGTVRWMATKKQKQKKENVDTKASKGRKIR 384
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,011,033
Number of Sequences: 5004
Number of extensions: 64463
Number of successful extensions: 216
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 202
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 214
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 323158234
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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