BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30015
(660 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g48760.1 68418.m06034 60S ribosomal protein L13A (RPL13aD) 136 2e-32
At3g07110.1 68416.m00847 60S ribosomal protein L13A (RPL13aA) si... 135 3e-32
At3g24830.1 68416.m03115 60S ribosomal protein L13A (RPL13aB) si... 132 3e-31
At4g13170.1 68417.m02049 60S ribosomal protein L13A (RPL13aC) ri... 131 3e-31
At1g61500.1 68414.m06929 S-locus protein kinase, putative simila... 29 3.6
At1g61420.1 68414.m06921 S-locus lectin protein kinase family pr... 29 3.6
At1g61380.1 68414.m06917 S-locus protein kinase, putative simila... 29 3.6
At3g10270.1 68416.m01231 DNA topoisomerase, ATP-hydrolyzing, put... 28 4.8
At1g61490.1 68414.m06928 S-locus protein kinase, putative simila... 28 4.8
At1g61460.1 68414.m06925 S-locus protein kinase, putative contai... 28 4.8
At5g48410.1 68418.m05986 glutamate receptor family protein (GLR1... 28 6.3
At1g58090.1 68414.m06583 F-box family protein contains F-box dom... 28 6.3
At5g11250.1 68418.m01314 disease resistance protein (TIR-NBS-LRR... 27 8.4
At5g03330.2 68418.m00285 OTU-like cysteine protease family prote... 27 8.4
At5g03330.1 68418.m00284 OTU-like cysteine protease family prote... 27 8.4
At1g49260.1 68414.m05523 hypothetical protein 27 8.4
>At5g48760.1 68418.m06034 60S ribosomal protein L13A (RPL13aD)
Length = 206
Score = 136 bits (328), Expect = 2e-32
Identities = 69/164 (42%), Positives = 88/164 (53%)
Frame = +3
Query: 3 GFSNKAIVIDGRGHLLGRLAAVIAKVLLEGNKVVVVRCEQINISGNFFRNKLKLMSFLRK 182
G +K +V+D R H+LGRLA++ AK LL G KVV+VRCE+I +SG R K+K M FLRK
Sbjct: 6 GICSKRVVVDARHHMLGRLASITAKELLNGQKVVIVRCEEICLSGGLVRQKMKYMRFLRK 65
Query: 183 RCNVNPARGPFHFRAPSKILWKTVEA*SHTKQSVARMLLGGYVHMMGALLPLITXXXXXX 362
R N P+ GP HFRAPSKI W+TV K L G P
Sbjct: 66 RMNTKPSHGPIHFRAPSKIFWRTVRGMIPHKTKRGAAALARLKVYEGVPTPYDKIKRMVI 125
Query: 363 XXXXXXXXXXXXXXXCHVGRLSHEIGWKYRDVVRKLEDKRKGKA 494
C +GRLS E+GW + D +++LE KRK +A
Sbjct: 126 PDALKVLRLQAGHKYCLLGRLSSEVGWNHYDTIKELETKRKERA 169
>At3g07110.1 68416.m00847 60S ribosomal protein L13A (RPL13aA)
similar to ribosomal protein L13A GB:O49885 [Lupinus
luteus]
Length = 206
Score = 135 bits (326), Expect = 3e-32
Identities = 69/164 (42%), Positives = 88/164 (53%)
Frame = +3
Query: 3 GFSNKAIVIDGRGHLLGRLAAVIAKVLLEGNKVVVVRCEQINISGNFFRNKLKLMSFLRK 182
G K +V+D R H+LGRLA+V+AK LL G +VVVRCE+I +SG R K+K M FLRK
Sbjct: 6 GICAKRVVVDARHHMLGRLASVVAKDLLNGQNIVVVRCEEICLSGGLVRQKMKYMRFLRK 65
Query: 183 RCNVNPARGPFHFRAPSKILWKTVEA*SHTKQSVARMLLGGYVHMMGALLPLITXXXXXX 362
R N P+ GP HFRAPSKI W+TV K L G P
Sbjct: 66 RMNTKPSHGPIHFRAPSKIFWRTVRGMIPHKTKRGANALARLKVFEGVPTPYDKIKRMVV 125
Query: 363 XXXXXXXXXXXXXXXCHVGRLSHEIGWKYRDVVRKLEDKRKGKA 494
C +GRLS E+GW + D +++LE+KRK +A
Sbjct: 126 PDALKVLRLQAGHKYCLLGRLSSEVGWNHYDTIKELENKRKERA 169
>At3g24830.1 68416.m03115 60S ribosomal protein L13A (RPL13aB)
similar to 60S RIBOSOMAL PROTEIN L13A GB:P35427 from
[Rattus norvegicus]
Length = 206
Score = 132 bits (318), Expect = 3e-31
Identities = 68/164 (41%), Positives = 87/164 (53%)
Frame = +3
Query: 3 GFSNKAIVIDGRGHLLGRLAAVIAKVLLEGNKVVVVRCEQINISGNFFRNKLKLMSFLRK 182
G +K +V+D R H+ GRLA++IAK LL G VVVVRCE+I +SG R K+K M FLRK
Sbjct: 6 GICSKRVVVDARHHMCGRLASIIAKELLNGQSVVVVRCEEICLSGGLVRQKMKYMRFLRK 65
Query: 183 RCNVNPARGPFHFRAPSKILWKTVEA*SHTKQSVARMLLGGYVHMMGALLPLITXXXXXX 362
R N P+ GP HFRAPSKI W+TV K L G P
Sbjct: 66 RMNTKPSHGPIHFRAPSKIFWRTVRGMIPHKTKRGAAALARLKVFEGVPPPYDKVKRMVI 125
Query: 363 XXXXXXXXXXXXXXXCHVGRLSHEIGWKYRDVVRKLEDKRKGKA 494
C +GRLS E+GW + D +++LE KRK ++
Sbjct: 126 PDALKVLRLQAGHKYCLLGRLSSEVGWNHYDTIKELEVKRKERS 169
>At4g13170.1 68417.m02049 60S ribosomal protein L13A (RPL13aC)
ribosomal protein L13a -Lupinus luteus,PID:e1237871
Length = 206
Score = 131 bits (317), Expect = 3e-31
Identities = 69/164 (42%), Positives = 87/164 (53%)
Frame = +3
Query: 3 GFSNKAIVIDGRGHLLGRLAAVIAKVLLEGNKVVVVRCEQINISGNFFRNKLKLMSFLRK 182
G K +V+DGR H+LGRLA+ AK LL G +VVVVRCE+I +SG R K+K M FLRK
Sbjct: 6 GICAKRVVVDGRHHMLGRLASNTAKELLNGQEVVVVRCEEICLSGGLVRQKMKYMRFLRK 65
Query: 183 RCNVNPARGPFHFRAPSKILWKTVEA*SHTKQSVARMLLGGYVHMMGALLPLITXXXXXX 362
R N P+ GP HFRAPSKI W+TV K L G P
Sbjct: 66 RMNTKPSHGPIHFRAPSKIFWRTVRGMIPHKTKRGAAALARLKVFEGIPPPYDKIKRMVI 125
Query: 363 XXXXXXXXXXXXXXXCHVGRLSHEIGWKYRDVVRKLEDKRKGKA 494
C +GRLS E+GW + D +++LE KRK ++
Sbjct: 126 PDALKVLRLQSGHKYCLLGRLSSEVGWNHYDTIKELETKRKERS 169
Score = 49.2 bits (112), Expect = 2e-06
Identities = 19/29 (65%), Positives = 24/29 (82%)
Frame = +2
Query: 254 RGMIPHKTERGKNALRRLRTYDGCPPPFD 340
RGMIPHKT+RG AL RL+ ++G PPP+D
Sbjct: 90 RGMIPHKTKRGAAALARLKVFEGIPPPYD 118
>At1g61500.1 68414.m06929 S-locus protein kinase, putative similar
to KI domain interacting kinase 1 [Zea mays]
gi|2735017|gb|AAB93834; contains S-locus glycoprotein
family domain, Pfam:PF00954
Length = 804
Score = 28.7 bits (61), Expect = 3.6
Identities = 21/90 (23%), Positives = 42/90 (46%)
Frame = -2
Query: 524 LSFFSWHSLNSLALPLVFKLTNNITVFPSNFMGQSAYMTVIYGQVLDRRHVEQQALQHVD 345
L FF H++ + TNN ++ SN +GQ + +V G++ D + + + L
Sbjct: 476 LDFFDMHTIQNA--------TNNFSL--SNKLGQGGFGSVYKGKLQDGKEIAVKRLSSSS 525
Query: 344 GYQREEGTHHMYVAS*EHSCHALFCVGSCL 255
G +EE + + + S + + +G C+
Sbjct: 526 GQGKEEFMNEIVLISKLQHRNLVRVLGCCI 555
>At1g61420.1 68414.m06921 S-locus lectin protein kinase family
protein contains Pfam domains, PF00954: S-locus
glycoprotein family, PF00069: Protein kinase domain, and
PF01453: Lectin (probable mannose binding)
Length = 807
Score = 28.7 bits (61), Expect = 3.6
Identities = 21/90 (23%), Positives = 41/90 (45%)
Frame = -2
Query: 524 LSFFSWHSLNSLALPLVFKLTNNITVFPSNFMGQSAYMTVIYGQVLDRRHVEQQALQHVD 345
L FF H++ + TNN ++ SN +GQ + V G++ D + + + L
Sbjct: 479 LDFFDMHTIQTA--------TNNFSI--SNKLGQGGFGPVYKGKLQDGKEIAVKRLSSSS 528
Query: 344 GYQREEGTHHMYVAS*EHSCHALFCVGSCL 255
G +EE + + + S + + +G C+
Sbjct: 529 GQGKEEFMNEIVLISKLQHKNLVRILGCCI 558
>At1g61380.1 68414.m06917 S-locus protein kinase, putative similar
to KI domain interacting kinase 1 [Zea mays]
gi|2735017|gb|AAB93834; contains S-locus glycoprotein
family domain, Pfam:PF00954
Length = 805
Score = 28.7 bits (61), Expect = 3.6
Identities = 19/82 (23%), Positives = 38/82 (46%)
Frame = -2
Query: 500 LNSLALPLVFKLTNNITVFPSNFMGQSAYMTVIYGQVLDRRHVEQQALQHVDGYQREEGT 321
+N + + TNN + PSN +GQ + V G+++D + + + L G EE
Sbjct: 473 VNFFEMHTIRTATNNFS--PSNKLGQGGFGPVYKGKLVDGKEIGVKRLASSSGQGTEEFM 530
Query: 320 HHMYVAS*EHSCHALFCVGSCL 255
+ + + S + + +G C+
Sbjct: 531 NEITLISKLQHRNLVRLLGYCI 552
>At3g10270.1 68416.m01231 DNA topoisomerase, ATP-hydrolyzing,
putative / DNA topoisomerase II, putative / DNA gyrase,
putative similar to SP|O50627 DNA gyrase subunit B (EC
5.99.1.3) {Bacillus halodurans}; contains Pfam profiles
PF02518: ATPase, histidine kinase-, DNA gyrase B-, and
HSP90-like domain protein, PF00204: DNA topoisomerase II
(N-terminal region), PF00986: DNA gyrase, B subunit,
carboxyl terminus, PF01751: Toprim domain
Length = 657
Score = 28.3 bits (60), Expect = 4.8
Identities = 15/52 (28%), Positives = 27/52 (51%)
Frame = +3
Query: 3 GFSNKAIVIDGRGHLLGRLAAVIAKVLLEGNKVVVVRCEQINISGNFFRNKL 158
G++N IDG H+ G A++ + K+ V++ + I++SG R L
Sbjct: 288 GYANSIRTIDGGTHIEGVKASLTRTLNSLAKKLKVIKEKDISLSGEHVREGL 339
>At1g61490.1 68414.m06928 S-locus protein kinase, putative similar
to KI domain interacting kinase 1 [Zea mays]
gi|2735017|gb|AAB93834; contains S-locus glycoprotein
family domain, Pfam:PF00954
Length = 804
Score = 28.3 bits (60), Expect = 4.8
Identities = 17/70 (24%), Positives = 35/70 (50%)
Frame = -2
Query: 464 TNNITVFPSNFMGQSAYMTVIYGQVLDRRHVEQQALQHVDGYQREEGTHHMYVAS*EHSC 285
TNN ++ SN +GQ + +V G++ D + + + L G +EE + + + S
Sbjct: 487 TNNFSL--SNKLGQGGFGSVYKGKLQDGKEIAVKQLSSSSGQGKEEFMNEIVLISKLQHR 544
Query: 284 HALFCVGSCL 255
+ + +G C+
Sbjct: 545 NLVRVLGCCI 554
>At1g61460.1 68414.m06925 S-locus protein kinase, putative contains
similarity to KI domain interacting kinase 1 [Zea mays]
gi|2735017|gb|AAB93834; contains S-locus glycoprotein
family domain, Pfam:PF00954
Length = 598
Score = 28.3 bits (60), Expect = 4.8
Identities = 17/70 (24%), Positives = 35/70 (50%)
Frame = -2
Query: 464 TNNITVFPSNFMGQSAYMTVIYGQVLDRRHVEQQALQHVDGYQREEGTHHMYVAS*EHSC 285
TNN ++ SN +GQ + +V G++ D + + + L G +EE + + + S
Sbjct: 300 TNNFSL--SNKLGQGGFGSVYKGKLQDGKEIAVKRLSSSSGQGKEEFMNEIVLISKLQHK 357
Query: 284 HALFCVGSCL 255
+ + +G C+
Sbjct: 358 NLVRILGCCI 367
>At5g48410.1 68418.m05986 glutamate receptor family protein (GLR1.3)
plant glutamate receptor family, PMID:11379626
Length = 860
Score = 27.9 bits (59), Expect = 6.3
Identities = 14/50 (28%), Positives = 25/50 (50%), Gaps = 3/50 (6%)
Frame = -2
Query: 647 GFIFWL*FLKSLNCIHRNLDDGSEMVF---VAFDTFSPASLVILLSFFSW 507
GF+ W+ K++N H N+DD ++ V F ++ P S+ + W
Sbjct: 259 GFV-WILTAKTMNSFHENIDDFTKQAMEGVVGFKSYIPMSIELQNFTLRW 307
>At1g58090.1 68414.m06583 F-box family protein contains F-box domain
Pfam:PF00646
Length = 371
Score = 27.9 bits (59), Expect = 6.3
Identities = 13/29 (44%), Positives = 18/29 (62%)
Frame = -2
Query: 596 NLDDGSEMVFVAFDTFSPASLVILLSFFS 510
N DDG ++V++ F T S ILLS+ S
Sbjct: 282 NGDDGDDVVWIKFMTVSRPDFPILLSYIS 310
>At5g11250.1 68418.m01314 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 1189
Score = 27.5 bits (58), Expect = 8.4
Identities = 16/39 (41%), Positives = 25/39 (64%)
Frame = -3
Query: 244 HRILDGALKWKGPRAGFTLHLLRRNDISLSLFLKKLPEI 128
H LD + W+G + LH LR+ D+S S+ LK+LP++
Sbjct: 662 HSKLD--MLWEGVKP---LHNLRQMDLSYSVNLKELPDL 695
>At5g03330.2 68418.m00285 OTU-like cysteine protease family protein
contains Pfam profile PF02338: OTU-like cysteine
protease
Length = 356
Score = 27.5 bits (58), Expect = 8.4
Identities = 14/50 (28%), Positives = 25/50 (50%)
Frame = +3
Query: 147 RNKLKLMSFLRKRCNVNPARGPFHFRAPSKILWKTVEA*SHTKQSVARML 296
RN+L++ F + P G FRA + L+KT + H ++ + + L
Sbjct: 205 RNRLEMFDFTEVKV---PGDGNCQFRALADQLYKTADRHKHVRRQIVKQL 251
>At5g03330.1 68418.m00284 OTU-like cysteine protease family protein
contains Pfam profile PF02338: OTU-like cysteine
protease
Length = 356
Score = 27.5 bits (58), Expect = 8.4
Identities = 14/50 (28%), Positives = 25/50 (50%)
Frame = +3
Query: 147 RNKLKLMSFLRKRCNVNPARGPFHFRAPSKILWKTVEA*SHTKQSVARML 296
RN+L++ F + P G FRA + L+KT + H ++ + + L
Sbjct: 205 RNRLEMFDFTEVKV---PGDGNCQFRALADQLYKTADRHKHVRRQIVKQL 251
>At1g49260.1 68414.m05523 hypothetical protein
Length = 149
Score = 27.5 bits (58), Expect = 8.4
Identities = 10/28 (35%), Positives = 14/28 (50%)
Frame = -3
Query: 298 KSILATLCFVWDHASTVFHRILDGALKW 215
K+I+ T C W H +T RI + W
Sbjct: 64 KAIITTCCSAWFHLTTYQERIEESLFSW 91
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,089,862
Number of Sequences: 28952
Number of extensions: 314997
Number of successful extensions: 858
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 833
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 852
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1383534864
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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