Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= tesS0002
(598 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 37 0.002
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 33 0.032
SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyce... 31 0.17
SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr ... 30 0.22
SPAC1F3.02c |mkh1||MEK kinase |Schizosaccharomyces pombe|chr 1||... 28 1.2
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 27 2.1
SPCC645.09 |mrpl37||mitochondrial ribosomal protein subunit L37|... 27 2.7
SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizos... 26 3.6
SPCC613.04c |rng3||UCS-domain protein |Schizosaccharomyces pombe... 26 4.8
SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi... 25 6.3
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 25 8.4
SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharo... 25 8.4
SPAC14C4.05c |mug61||Sad1 interacting factor|Schizosaccharomyces... 25 8.4
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 37.1 bits (82), Expect = 0.002
Identities = 16/53 (30%), Positives = 28/53 (52%)
Frame = +3
Query: 309 FRRMDDDGSKLLNKEEFLYGIKETGLELNKSEAEELFSQFDTDSSGSISLDEF 467
F+ D DG+ + EE + + G L++ E ++ + DTD G I+ +EF
Sbjct: 91 FKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREADTDGDGVINYEEF 143
Score = 28.7 bits (61), Expect = 0.68
Identities = 14/60 (23%), Positives = 29/60 (48%)
Frame = +3
Query: 321 DDDGSKLLNKEEFLYGIKETGLELNKSEAEELFSQFDTDSSGSISLDEFLIKIRPPMSES 500
D DG+ + E ++ G +E +++ ++ D D +G+I EFL + M ++
Sbjct: 24 DQDGN--ITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDFTEFLTMMARKMKDT 81
Score = 27.5 bits (58), Expect = 1.6
Identities = 10/21 (47%), Positives = 16/21 (76%)
Frame = +2
Query: 512 VEQAFKKLDKTGDGAITIDDI 574
V +AFK DK G+G IT++++
Sbjct: 87 VREAFKVFDKDGNGYITVEEL 107
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 33.1 bits (72), Expect = 0.032
Identities = 16/56 (28%), Positives = 25/56 (44%)
Frame = +3
Query: 303 RIFRRMDDDGSKLLNKEEFLYGIKETGLELNKSEAEELFSQFDTDSSGSISLDEFL 470
R F DDD + ++ KE ++ E E + +FD D G I+ EF+
Sbjct: 114 RAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEEFDLDQDGEINEQEFI 169
Score = 31.1 bits (67), Expect = 0.13
Identities = 17/76 (22%), Positives = 32/76 (42%), Gaps = 6/76 (7%)
Frame = +3
Query: 297 LGRIFRRMDDDGSKLLNKEEFLYGIKETGLELNKSEAEELFSQFDTDSSGSISLDEFL-- 470
+ F+ D D ++ E ++ G KSE ++ FD G + +++F+
Sbjct: 39 INEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQMEDFVRV 98
Query: 471 ----IKIRPPMSESRR 506
I R P+ E +R
Sbjct: 99 MTEKIVERDPLEEIKR 114
>SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 174
Score = 30.7 bits (66), Expect = 0.17
Identities = 18/70 (25%), Positives = 35/70 (50%), Gaps = 1/70 (1%)
Frame = +3
Query: 303 RIFRRMDDDGSKLLNKEEFLYGIKETGLELNKSEAEEL-FSQFDTDSSGSISLDEFLIKI 479
R+F +D+DG ++ +EF+ + + NK E + F +D D G IS E + +
Sbjct: 60 RLFSVVDEDGGGDVDFQEFINSLSVFSVHGNKEEKLKFAFKIYDIDRDGYISNGELYLVL 119
Query: 480 RPPMSESRRK 509
+ + + R+
Sbjct: 120 KMMVGTNLRE 129
>SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr
1|||Manual
Length = 621
Score = 30.3 bits (65), Expect = 0.22
Identities = 14/51 (27%), Positives = 23/51 (45%)
Frame = +3
Query: 294 GLGRIFRRMDDDGSKLLNKEEFLYGIKETGLELNKSEAEELFSQFDTDSSG 446
G+ ++FR + S +LN+ EF + GL + E LF + G
Sbjct: 488 GITKVFRHFEKKKSNMLNEVEFYAALASLGLVYDTEEGTALFHRAANSEEG 538
>SPAC1F3.02c |mkh1||MEK kinase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1116
Score = 27.9 bits (59), Expect = 1.2
Identities = 15/34 (44%), Positives = 19/34 (55%), Gaps = 1/34 (2%)
Frame = -2
Query: 579 PLMSSIVIAPSPVLSSFLNACSTI-YDAIPTWED 481
P+ S +V SP FLNAC T+ D PT E+
Sbjct: 1054 PIPSELVSQVSPEAIQFLNACFTVNADVRPTAEE 1087
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 27.1 bits (57), Expect = 2.1
Identities = 12/25 (48%), Positives = 17/25 (68%)
Frame = +2
Query: 512 VEQAFKKLDKTGDGAITIDDIKGCL 586
+++AF LDK GDG I +D+K L
Sbjct: 50 LKEAFALLDKDGDGNIGREDVKTML 74
>SPCC645.09 |mrpl37||mitochondrial ribosomal protein subunit
L37|Schizosaccharomyces pombe|chr 3|||Manual
Length = 139
Score = 26.6 bits (56), Expect = 2.7
Identities = 16/48 (33%), Positives = 23/48 (47%)
Frame = +1
Query: 346 TKKNSFMASRKQGWNLIKVKPKNSSVNSTQTVVAQSVLMNSLLKSVLP 489
+ +NS + K+ + +V PK NS T AQ V + SVLP
Sbjct: 32 SSRNSSSSLVKRSYVSSRVSPKKPQHNSDATSSAQKVANKTHTSSVLP 79
>SPAC22F8.11 |plc1||phosphoinositide phospholipase C
Plc1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 899
Score = 26.2 bits (55), Expect = 3.6
Identities = 14/30 (46%), Positives = 17/30 (56%), Gaps = 2/30 (6%)
Frame = +3
Query: 384 LELNKSEA--EELFSQFDTDSSGSISLDEF 467
L LN S EE F + D D SG +S +EF
Sbjct: 326 LHLNASMEFLEETFQKADADHSGKLSFEEF 355
>SPCC613.04c |rng3||UCS-domain protein |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 746
Score = 25.8 bits (54), Expect = 4.8
Identities = 10/34 (29%), Positives = 21/34 (61%), Gaps = 1/34 (2%)
Frame = -2
Query: 366 HKGIL-LCSVVYFHHHPCDGKFFQVPRSLKHLGT 268
H+G++ +C++VY KF + P++++ L T
Sbjct: 686 HRGLVCICNIVYSKDQEIFNKFIKTPKAVETLRT 719
>SPAC18B11.04 |ncs1||related to neuronal calcium sensor
Ncs1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 190
Score = 25.4 bits (53), Expect = 6.3
Identities = 17/44 (38%), Positives = 23/44 (52%), Gaps = 1/44 (2%)
Frame = +3
Query: 342 LNKEEFLYGIKETGLELNKSE-AEELFSQFDTDSSGSISLDEFL 470
LNK EF K+ + S AE +F+ FD D +G I EF+
Sbjct: 43 LNKSEFQKIYKQFFPFGDPSAFAEYVFNVFDADKNGYIDFKEFI 86
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 25.0 bits (52), Expect = 8.4
Identities = 13/36 (36%), Positives = 21/36 (58%), Gaps = 1/36 (2%)
Frame = +3
Query: 306 IFRRMDDDGSKLLNKEEFLYGIKETGL-ELNKSEAE 410
IFRR+ D S + ++ FL+ G+ E+ +EAE
Sbjct: 377 IFRRLGDQFSAMFRRKAFLHWYTGEGMDEMEFTEAE 412
>SPBC215.01 ||SPBC3B9.20|GTPase activating
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 834
Score = 25.0 bits (52), Expect = 8.4
Identities = 11/39 (28%), Positives = 20/39 (51%)
Frame = +3
Query: 354 EFLYGIKETGLELNKSEAEELFSQFDTDSSGSISLDEFL 470
E+ G+ + + S LF +FD +GS+SL + +
Sbjct: 564 EWAKGLDAAAINNSSSFLRHLFLRFDKSMTGSLSLQDLV 602
>SPAC14C4.05c |mug61||Sad1 interacting factor|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 844
Score = 25.0 bits (52), Expect = 8.4
Identities = 14/38 (36%), Positives = 20/38 (52%)
Frame = +3
Query: 396 KSEAEELFSQFDTDSSGSISLDEFLIKIRPPMSESRRK 509
K++ LFS+ +G IS+ E K PP S S R+
Sbjct: 38 KAQLITLFSKLRRAKNGLISMTELQQKNVPPSSRSPRR 75
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,290,981
Number of Sequences: 5004
Number of extensions: 44360
Number of successful extensions: 180
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 165
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 179
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 260219058
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -