BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= prgv0149 (659 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 163 2e-41 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 93 4e-20 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 81 1e-16 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 74 2e-14 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 72 9e-14 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 48 2e-06 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 45 1e-05 SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 44 1e-05 SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 39 6e-04 SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 28 1.4 SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3 |Schizosaccha... 27 2.4 SPAC1783.05 |hrp1|chd1|ATP-dependent DNA helicase Hrp1|Schizosac... 25 9.7 SPAC1B1.02c |||NAD/NADH kinase |Schizosaccharomyces pombe|chr 1|... 25 9.7 SPCC1682.02c |mcm3||MCM complex subunit Mcm3|Schizosaccharomyces... 25 9.7 SPBC2F12.03c |||EST1 family protein|Schizosaccharomyces pombe|ch... 25 9.7 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 163 bits (396), Expect = 2e-41 Identities = 73/82 (89%), Positives = 79/82 (96%) Frame = +1 Query: 256 YANTVMSGGTTMYPGIADRMQKEITALAPSTIKIKIIAPPERKYSVWIGGSILASLSTFQ 435 Y N VMSGGTTMYPGIADRMQKEI ALAPS++K+KI+APPERKYSVWIGGSILASLSTFQ Sbjct: 294 YGNVVMSGGTTMYPGIADRMQKEIQALAPSSMKVKIVAPPERKYSVWIGGSILASLSTFQ 353 Query: 436 QMWISKEEYDESGPGIVHRKCF 501 QMWISK+EYDESGPGIV+RKCF Sbjct: 354 QMWISKQEYDESGPGIVYRKCF 375 Score = 154 bits (373), Expect = 1e-38 Identities = 72/84 (85%), Positives = 76/84 (90%) Frame = +2 Query: 2 VRDIKEKLCYVALDFEQEMATAAASTSLEKSYELPXGQVITIGNERFRCPEALFQPSFLG 181 VRDIKEKLCYVALDFEQE+ TAA S+SLEKSYELP GQVITIGNERFR PEALFQPS LG Sbjct: 209 VRDIKEKLCYVALDFEQELQTAAQSSSLEKSYELPDGQVITIGNERFRAPEALFQPSALG 268 Query: 182 MESCGIHETVYNSIMKCDVDIRKD 253 +E+ GIHE YNSIMKCDVDIRKD Sbjct: 269 LENAGIHEATYNSIMKCDVDIRKD 292 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 92.7 bits (220), Expect = 4e-20 Identities = 40/83 (48%), Positives = 58/83 (69%) Frame = +2 Query: 2 VRDIKEKLCYVALDFEQEMATAAASTSLEKSYELPXGQVITIGNERFRCPEALFQPSFLG 181 VR++KEKLCYV+ D E + + +T L ++Y LP G+VI +G+ER+ CPE LFQP +G Sbjct: 209 VREMKEKLCYVSYDLELDHKLSEETTVLMRNYTLPDGRVIKVGSERYECPECLFQPHLVG 268 Query: 182 MESCGIHETVYNSIMKCDVDIRK 250 E G+ E ++++I DVDIRK Sbjct: 269 SEQPGLSEFIFDTIQAADVDIRK 291 Score = 56.8 bits (131), Expect = 3e-09 Identities = 32/85 (37%), Positives = 52/85 (61%), Gaps = 12/85 (14%) Frame = +1 Query: 256 YANTVMSGGTTMYPGIADRMQKEITAL--------APSTI---KIKIIAPPERKYSVWIG 402 Y V+SGG++MY G+ R++KEI L P+ + K+KI P R+++V+IG Sbjct: 294 YRAIVLSGGSSMYAGLPSRLEKEIKQLWFERVLHGDPARLPNFKVKIEDAPRRRHAVFIG 353 Query: 403 GSILAS-LSTFQQMWISKEEYDESG 474 G++LA ++ MW+SK E++E G Sbjct: 354 GAVLADIMAQNDHMWVSKAEWEEYG 378 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 81.0 bits (191), Expect = 1e-16 Identities = 36/82 (43%), Positives = 50/82 (60%) Frame = +2 Query: 2 VRDIKEKLCYVALDFEQEMATAAASTSLEKSYELPXGQVITIGNERFRCPEALFQPSFLG 181 VR+IKEK CYVA DF E+ + +Y+LP Q IT+G E F PE LF P +G Sbjct: 214 VREIKEKCCYVASDFRSEIESWTEHKPQIHTYQLPDNQTITLGTECFSAPEVLFNPEMMG 273 Query: 182 MESCGIHETVYNSIMKCDVDIR 247 E+ G+H ++ SI+ D+D+R Sbjct: 274 SEASGLHIQLFKSILLSDIDLR 295 Score = 77.8 bits (183), Expect = 1e-15 Identities = 35/82 (42%), Positives = 57/82 (69%) Frame = +1 Query: 256 YANTVMSGGTTMYPGIADRMQKEITALAPSTIKIKIIAPPERKYSVWIGGSILASLSTFQ 435 Y+N V+SGG+T+ G +R E+ A++ ++KI A PER ++ W+GGSILASLSTF+ Sbjct: 299 YSNIVLSGGSTLLRGFGERFISELRAISGKKNQVKIYASPERMHNAWLGGSILASLSTFR 358 Query: 436 QMWISKEEYDESGPGIVHRKCF 501 ++ I+ EEY ++ ++ R+ F Sbjct: 359 RLLITSEEY-KNDQNVIFRRRF 379 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 73.7 bits (173), Expect = 2e-14 Identities = 35/85 (41%), Positives = 57/85 (67%), Gaps = 6/85 (7%) Frame = +1 Query: 262 NTVMSGGTTMYPGIADRMQKEITALAPSTIKIKIIAPP---ERKYSVWIGGSILASLSTF 432 N ++ GGT++ G + R+Q E++ L P + ++KI A ER Y+ W+GGSIL+SL TF Sbjct: 349 NVIVCGGTSLMQGFSLRLQNELSKLYPGS-RLKIHASGHVVERSYASWLGGSILSSLGTF 407 Query: 433 QQMWISKEEYDESGP---GIVHRKC 498 Q+WIS++EY+E G ++ ++C Sbjct: 408 HQLWISRQEYEEHGSDRLALIEKRC 432 Score = 31.5 bits (68), Expect(2) = 0.003 Identities = 13/35 (37%), Positives = 20/35 (57%) Frame = +2 Query: 65 AAASTSLEKSYELPXGQVITIGNERFRCPEALFQP 169 ++ +T E +E P G + G ER++ PE LF P Sbjct: 254 SSETTIAESEFEFPDGSRMMFGAERYQIPEHLFVP 288 Score = 24.2 bits (50), Expect(2) = 0.003 Identities = 10/18 (55%), Positives = 12/18 (66%) Frame = +2 Query: 194 GIHETVYNSIMKCDVDIR 247 GI + N I +CDVDIR Sbjct: 326 GIPQLFQNCISECDVDIR 343 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 71.7 bits (168), Expect = 9e-14 Identities = 34/73 (46%), Positives = 48/73 (65%), Gaps = 2/73 (2%) Frame = +1 Query: 262 NTVMSGGTTMYPGIADRMQKEITALAP-STIKIKIIAPPER-KYSVWIGGSILASLSTFQ 435 N V++GGT++ PG+++R+Q E+ LA S I + +VW GGSILASL FQ Sbjct: 346 NIVVTGGTSLIPGLSERLQAEVQRLATGSRINVHTAETASATSNAVWFGGSILASLDNFQ 405 Query: 436 QMWISKEEYDESG 474 +W+SK+EYDE G Sbjct: 406 HLWVSKQEYDEVG 418 Score = 51.2 bits (117), Expect = 1e-07 Identities = 29/70 (41%), Positives = 35/70 (50%), Gaps = 9/70 (12%) Frame = +2 Query: 65 AAASTSLEKSYELPXGQVITIGNERFRCPEALFQPSFLGMESC---------GIHETVYN 217 A AS+ K +E P G G ERFR E LF PSF S G+HE VY Sbjct: 271 AKASSRNPKPFEFPDGVTHKFGQERFRISEILFNPSFSASRSAETTPPQGSVGLHELVYQ 330 Query: 218 SIMKCDVDIR 247 SI+ CD ++R Sbjct: 331 SILACDSELR 340 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 47.6 bits (108), Expect = 2e-06 Identities = 26/95 (27%), Positives = 46/95 (48%), Gaps = 14/95 (14%) Frame = +1 Query: 250 GPYANTVMSGGTTMYPGIADRMQKEITALAPSTIK--------------IKIIAPPERKY 387 G Y N V+SGG+T++ +R+Q+++ + I + +I+ ++ Sbjct: 325 GLYKNIVLSGGSTLFKNFGNRLQRDLKRIVDERIHRSEMLSGAKSGGVDVNVISHKRQRN 384 Query: 388 SVWIGGSILASLSTFQQMWISKEEYDESGPGIVHR 492 +VW GGS+LA F +K +Y+E G I R Sbjct: 385 AVWFGGSLLAQTPEFGSYCHTKADYEEYGASIARR 419 Score = 35.9 bits (79), Expect = 0.005 Identities = 27/85 (31%), Positives = 37/85 (43%), Gaps = 5/85 (5%) Frame = +2 Query: 11 IKEKLCYVALDFEQEMATAAASTS--LEKSYELPXGQVITI--GNERFRCPEALFQPSFL 178 IKE+ CYV D +E + L+ + E G TI G ERF PE F P Sbjct: 240 IKEECCYVCPDIVKEFSRFDREPDRYLKYASESITGHSTTIDVGFERFLAPEIFFNPEIA 299 Query: 179 GME-SCGIHETVYNSIMKCDVDIRK 250 + + E V N + +D+RK Sbjct: 300 SSDFLTPLPELVDNVVQSSPIDVRK 324 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 44.8 bits (101), Expect = 1e-05 Identities = 20/73 (27%), Positives = 37/73 (50%) Frame = +1 Query: 259 ANTVMSGGTTMYPGIADRMQKEITALAPSTIKIKIIAPPERKYSVWIGGSILASLSTFQQ 438 +N +++GG PG+ R+++E+T++ P I + W G S + F+ Sbjct: 636 SNVLITGGLGSLPGMETRIKRELTSIMPVGSSINVFRASNPLLDAWKGASEWSVTEKFKA 695 Query: 439 MWISKEEYDESGP 477 +++EEY E GP Sbjct: 696 AKVTREEYLEKGP 708 Score = 30.3 bits (65), Expect = 0.26 Identities = 13/32 (40%), Positives = 21/32 (65%) Frame = +2 Query: 134 ERFRCPEALFQPSFLGMESCGIHETVYNSIMK 229 E+ R PE +F PS +G++ GI E + SI++ Sbjct: 594 EQIRVPEVIFSPSIVGIDQAGILE-IMRSILQ 624 >SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces pombe|chr 3|||Manual Length = 401 Score = 44.4 bits (100), Expect = 1e-05 Identities = 31/94 (32%), Positives = 44/94 (46%), Gaps = 20/94 (21%) Frame = +2 Query: 2 VRDIKEKLCYVALDFEQEMATAAAS--TSLEKSYELPX------------------GQVI 121 V +IKE +CYV+ +F+++M + LE Y LP QV+ Sbjct: 215 VNEIKESVCYVSQNFKEDMEICHEKPRSKLEICYALPDYSTGKHGYIVRDINQKIEQQVL 274 Query: 122 TIGNERFRCPEALFQPSFLGMESCGIHETVYNSI 223 + NERF PE LF PS + + GI E V S+ Sbjct: 275 NLSNERFMIPELLFSPSDIEIREAGIPEAVMQSV 308 Score = 44.4 bits (100), Expect = 1e-05 Identities = 24/78 (30%), Positives = 38/78 (48%) Frame = +1 Query: 262 NTVMSGGTTMYPGIADRMQKEITALAPSTIKIKIIAPPERKYSVWIGGSILASLSTFQQM 441 N V GG +PG R+ E+ +LAP+ ++K+ P + W S + L + Sbjct: 322 NVVTIGGNCKFPGFHKRLSSELRSLAPANWEVKVFEPSDPICFPWKKASHM-PLEHWNAN 380 Query: 442 WISKEEYDESGPGIVHRK 495 I++ EY E G I+ RK Sbjct: 381 KITRSEYSEHGANIMTRK 398 >SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pombe|chr 1|||Manual Length = 620 Score = 39.1 bits (87), Expect = 6e-04 Identities = 18/83 (21%), Positives = 39/83 (46%), Gaps = 3/83 (3%) Frame = +1 Query: 256 YANTVMSGGTTMYPGIADRMQKEITALAPSTIKIKIIAPP---ERKYSVWIGGSILASLS 426 Y++ ++ GG +PG A +++ I + + I +I PP + ++ W G I + Sbjct: 530 YSSILIVGGAGQFPGFAHLLEERIHSKRANIPTISVIPPPRSMDAQFVAWKGACIYNRIR 589 Query: 427 TFQQMWISKEEYDESGPGIVHRK 495 ++WI ++ G ++ K Sbjct: 590 IVSELWIKNSDWKMLGSRVLQYK 612 >SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosaccharomyces pombe|chr 1|||Manual Length = 523 Score = 27.9 bits (59), Expect = 1.4 Identities = 13/37 (35%), Positives = 23/37 (62%), Gaps = 3/37 (8%) Frame = +1 Query: 394 WIGGSILASLSTFQQM---WISKEEYDESGPGIVHRK 495 ++GGSI+A S + + +++ EEY + GP +H K Sbjct: 486 FLGGSIVAKTSFNESVSSHYVTLEEYAQHGPTAIHTK 522 >SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 617 Score = 27.1 bits (57), Expect = 2.4 Identities = 14/37 (37%), Positives = 20/37 (54%) Frame = +1 Query: 319 KEITALAPSTIKIKIIAPPERKYSVWIGGSILASLST 429 KEI++ A S I + + P E+ W ILA L+T Sbjct: 212 KEISSQARSNIALPTLRPQEKNLIDWRDRKILAPLTT 248 >SPAC1783.05 |hrp1|chd1|ATP-dependent DNA helicase Hrp1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1373 Score = 25.0 bits (52), Expect = 9.7 Identities = 7/17 (41%), Positives = 11/17 (64%) Frame = -2 Query: 265 CWRTVLTDVDVALHDGV 215 CW+ + D D+ LHD + Sbjct: 1151 CWQAIKNDPDLGLHDKI 1167 >SPAC1B1.02c |||NAD/NADH kinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 537 Score = 25.0 bits (52), Expect = 9.7 Identities = 14/45 (31%), Positives = 25/45 (55%) Frame = +1 Query: 244 P*GPYANTVMSGGTTMYPGIADRMQKEITALAPSTIKIKIIAPPE 378 P G A +V +GG+ +PGI + I+A+ P ++ + I P+ Sbjct: 405 PTGSTAYSVAAGGSLCHPGIPAIL---ISAICPHSLSFRPIILPD 446 >SPCC1682.02c |mcm3||MCM complex subunit Mcm3|Schizosaccharomyces pombe|chr 3|||Manual Length = 879 Score = 25.0 bits (52), Expect = 9.7 Identities = 11/24 (45%), Positives = 15/24 (62%) Frame = +3 Query: 345 DHQDQDHRSPREEVLRMDRWIHPG 416 D D+ R+ E VLRM R++ PG Sbjct: 499 DIDDKKDRALSEHVLRMHRYLPPG 522 >SPBC2F12.03c |||EST1 family protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 891 Score = 25.0 bits (52), Expect = 9.7 Identities = 10/22 (45%), Positives = 13/22 (59%) Frame = -1 Query: 572 LTVAFRTRTSTTPHSTGFTRPP 507 L V R +TP+ST F+ PP Sbjct: 688 LYVLLNMRAKSTPNSTSFSSPP 709 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,291,034 Number of Sequences: 5004 Number of extensions: 42631 Number of successful extensions: 132 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 111 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 125 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 299817502 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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