Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fbVf0032
(636 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g38840.1 68415.m04772 guanylate-binding family protein simila... 47 1e-05
At5g46070.1 68418.m05665 guanylate-binding family protein contai... 32 0.37
At2g36110.1 68415.m04434 3'-5' exonuclease domain-containing pro... 31 0.85
At5g04560.1 68418.m00456 DEMETER protein (DME) identical to DEME... 29 2.6
At4g36980.1 68417.m05240 expressed protein 29 2.6
At1g60850.3 68414.m06850 DNA-directed RNA polymerase, putative i... 29 3.4
At4g28670.1 68417.m04097 protein kinase family protein contains ... 28 4.5
At5g54260.1 68418.m06759 DNA repair and meiosis protein (Mre11) ... 28 6.0
At5g20360.1 68418.m02422 octicosapeptide/Phox/Bem1p (PB1) domain... 28 6.0
At5g08080.1 68418.m00942 syntaxin, putative (SYP132) similar to ... 28 6.0
At1g14620.1 68414.m01738 expressed protein 28 6.0
At5g50450.1 68418.m06247 zinc finger (MYND type) family protein ... 27 7.9
At5g14650.1 68418.m01716 polygalacturonase, putative / pectinase... 27 7.9
At4g18465.1 68417.m02740 RNA helicase, putative similar to SP|Q1... 27 7.9
At1g68660.1 68414.m07845 expressed protein 27 7.9
>At2g38840.1 68415.m04772 guanylate-binding family protein similar
to SP|Q01514 Interferon-induced guanylate-binding
protein 1 (Guanine nucleotide-binding protein 1)
(Interferon-gamma inducible protein MAG-1) {Mus
musculus}; contains Pfam profile PF02263:
Guanylate-binding protein, N-terminal domain
Length = 679
Score = 46.8 bits (106), Expect = 1e-05
Identities = 30/77 (38%), Positives = 46/77 (59%), Gaps = 2/77 (2%)
Frame = -1
Query: 504 QGFSWRGGSERDTTGILMWSEIFKATLDDGEKVAIILLDTQGAFDS--ESTVRDCATVFA 331
+GF + T GI +W + +D G K ++I LDT+G F+S +S V D +FA
Sbjct: 87 EGFGVGHMRDTKTKGIWVWGTPLELEID-GVKTSVIYLDTEG-FESVGKSNVYD-DRIFA 143
Query: 330 LSTMLSSVQIYNLSQNI 280
L+T++SSV IYNL + +
Sbjct: 144 LATVMSSVLIYNLPETV 160
>At5g46070.1 68418.m05665 guanylate-binding family protein contains
Pfam domains PF02263: Guanylate-binding protein,
N-terminal domain and PF02841: Guanylate-binding
protein, C-terminal domain
Length = 1060
Score = 31.9 bits (69), Expect = 0.37
Identities = 21/65 (32%), Positives = 32/65 (49%), Gaps = 2/65 (3%)
Frame = -1
Query: 513 GPLQGFSWRGGSERDTTGILMWSEIFKATLDDGEKVAIILLDTQG--AFDSESTVRDCAT 340
G GF + T G+ +WS K T DG + ++LLD++G A+D D A+
Sbjct: 92 GRSNGFQVASTHKPCTKGLWLWSSPIKRTALDGTEYNLLLLDSEGIDAYDQTMGGIDEAS 151
Query: 339 VFALS 325
+ LS
Sbjct: 152 LDRLS 156
>At2g36110.1 68415.m04434 3'-5' exonuclease domain-containing
protein contains Pfam profile PF01612: 3'-5' exonuclease
Length = 239
Score = 30.7 bits (66), Expect = 0.85
Identities = 17/42 (40%), Positives = 22/42 (52%)
Frame = -3
Query: 376 LRQ*IHGARLRDRVRSVHDAVVRSDIQPLAKHPGGRPPAPSL 251
+R+ IH R R+RS H VV D+Q PGG P P +
Sbjct: 37 IRRWIHSIRFFSRLRSSHPLVVGLDVQ---WTPGGSDPPPDI 75
>At5g04560.1 68418.m00456 DEMETER protein (DME) identical to DEMETER
protein [Arabidopsis thaliana] GI:21743571; contains Pfam
profile PF00730: HhH-GPD superfamily base excision DNA
repair protein
Length = 1729
Score = 29.1 bits (62), Expect = 2.6
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = -2
Query: 212 PDSLPAAAVPRARLELPLRSALRSG 138
P+S P A+P L LPL +L SG
Sbjct: 1418 PESYPPVAIPMIELPLPLEKSLASG 1442
>At4g36980.1 68417.m05240 expressed protein
Length = 560
Score = 29.1 bits (62), Expect = 2.6
Identities = 13/28 (46%), Positives = 18/28 (64%)
Frame = -2
Query: 182 RARLELPLRSALRSGRRHADTVKKIKGI 99
R+R P RS RS +RHAD + I+G+
Sbjct: 518 RSRSRSPSRSLSRSPKRHADALHLIRGL 545
>At1g60850.3 68414.m06850 DNA-directed RNA polymerase, putative
identical to RNA polymerase subunit [Arabidopsis
thaliana] GI:514322; contains Pfam profile PF01000: RNA
polymerase Rpb3/RpoA insert domain
Length = 302
Score = 28.7 bits (61), Expect = 3.4
Identities = 11/31 (35%), Positives = 20/31 (64%), Gaps = 1/31 (3%)
Frame = -3
Query: 94 KQHPELQSLRKHITACFSELA-CFLMPHPGL 5
+ HPE++S+ +++ E A CF+ PHP +
Sbjct: 269 RMHPEVRSIYINLSYALWETALCFVFPHPNI 299
>At4g28670.1 68417.m04097 protein kinase family protein contains
Pfam domain, PF00069: Protein kinase domain
Length = 625
Score = 28.3 bits (60), Expect = 4.5
Identities = 12/28 (42%), Positives = 17/28 (60%)
Frame = -1
Query: 462 GILMWSEIFKATLDDGEKVAIILLDTQG 379
G+ + E+FK TL DG ++AI L G
Sbjct: 338 GVGGYGEVFKGTLSDGREIAIKRLHVSG 365
>At5g54260.1 68418.m06759 DNA repair and meiosis protein (Mre11)
identical to DNA repair and meiosis protein (Mre11)
GI:5524769 from [Arabidopsis thaliana]
Length = 720
Score = 27.9 bits (59), Expect = 6.0
Identities = 11/23 (47%), Positives = 15/23 (65%)
Frame = +3
Query: 48 HAVMCLRRDCSSGCCLSDTFNLL 116
HAV +R + GC +SD FN+L
Sbjct: 197 HAVQWMRPEVQEGCDVSDWFNIL 219
>At5g20360.1 68418.m02422 octicosapeptide/Phox/Bem1p (PB1)
domain-containing protein / tetratricopeptide repeat
(TPR)-containing protein contains Pfam profiles PF00564:
PB1 domain, PF00515: TPR Domain
Length = 809
Score = 27.9 bits (59), Expect = 6.0
Identities = 10/35 (28%), Positives = 19/35 (54%)
Frame = -3
Query: 139 DGGMQILSRRLKVSDKQHPELQSLRKHITACFSEL 35
DG M +K+ K H E+ +R ++ +C+ +L
Sbjct: 143 DGAMFKYGEAIKILPKDHVEVSHVRANVASCYMQL 177
>At5g08080.1 68418.m00942 syntaxin, putative (SYP132) similar to
SP|Q9ZSD4 Syntaxin 121 (AtSYP121) (Syntaxin-related
protein At-Syr1) {Arabidopsis thaliana}
Length = 304
Score = 27.9 bits (59), Expect = 6.0
Identities = 12/36 (33%), Positives = 21/36 (58%)
Frame = -3
Query: 145 GADGGMQILSRRLKVSDKQHPELQSLRKHITACFSE 38
G D G++ ++++V DKQ+ +L L K + A E
Sbjct: 29 GGDQGLEDFFKKVQVIDKQYDKLDKLLKKLQASHEE 64
>At1g14620.1 68414.m01738 expressed protein
Length = 233
Score = 27.9 bits (59), Expect = 6.0
Identities = 11/19 (57%), Positives = 13/19 (68%)
Frame = +2
Query: 155 FVGEAPVAHEELQPLEGSP 211
FVG AP+AH +QP E P
Sbjct: 162 FVGNAPMAHMAIQPTEEMP 180
>At5g50450.1 68418.m06247 zinc finger (MYND type) family protein
contains Pfam profile PF01753: MYND finger
Length = 336
Score = 27.5 bits (58), Expect = 7.9
Identities = 13/42 (30%), Positives = 19/42 (45%)
Frame = -1
Query: 504 QGFSWRGGSERDTTGILMWSEIFKATLDDGEKVAIILLDTQG 379
Q WR + + T + +W DDGE VA+ + D G
Sbjct: 293 QALDWRAKHKVECTPLDLWVAAAAEIGDDGEAVAVEIDDNHG 334
>At5g14650.1 68418.m01716 polygalacturonase, putative / pectinase,
putative similar to polygalacturonase PG1 GP|5669846,
PG2 GI:5669848 from [Glycine max]; contains PF00295:
Glycosyl hydrolases family 28 (polygalacturonases)
Length = 435
Score = 27.5 bits (58), Expect = 7.9
Identities = 23/104 (22%), Positives = 42/104 (40%), Gaps = 4/104 (3%)
Frame = -1
Query: 507 LQGFSWRGGSERDTTGILMWSEIFKATLDDGEKVAIILLDTQGAFDSESTVRDCATVFAL 328
LQG + +G D G + W+++ + + A+ + G S T+++
Sbjct: 139 LQGITIKGKGIIDGRGSVWWNDMMGTKMPRTKPTALRFYGSNGVTVSGITIQNSPQTHLK 198
Query: 327 STMLSSVQIYNLSQNIQED----DLQHLHCSQSTVGWRSRTGAG 208
S+Q+ + + + D D HL SQ V +RS G
Sbjct: 199 FDNCISIQVSDFTTSSPGDSPNTDGIHLQNSQDAVIYRSTLACG 242
>At4g18465.1 68417.m02740 RNA helicase, putative similar to
SP|Q14562 ATP-dependent helicase DDX8 (RNA helicase
HRH1) (DEAH-box protein 8) {Homo sapiens}; contains Pfam
profiles PF04408: Helicase associated domain (HA2),
PF00271: Helicase conserved C-terminal domain
Length = 704
Score = 27.5 bits (58), Expect = 7.9
Identities = 14/37 (37%), Positives = 24/37 (64%), Gaps = 1/37 (2%)
Frame = -3
Query: 121 LSRRLKVSDKQ-HPELQSLRKHITACFSELACFLMPH 14
++RRL ++ K +++++RK +TA F AC L PH
Sbjct: 597 IARRLGITLKSCDGDMEAVRKAVTAGFFANACRLEPH 633
>At1g68660.1 68414.m07845 expressed protein
Length = 159
Score = 27.5 bits (58), Expect = 7.9
Identities = 12/42 (28%), Positives = 24/42 (57%)
Frame = +2
Query: 368 LSKAPCVSSNIIATFSPSSSVALNISDHMSMPVVSLSEPPRQ 493
+SK PCV+ +I+ T S S+++ + P++ + P R+
Sbjct: 27 VSKGPCVNRSILMTLSTSAALGKG-GGVLDKPIIEKTTPGRE 67
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,971,858
Number of Sequences: 28952
Number of extensions: 251844
Number of successful extensions: 860
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 838
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 860
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1305036432
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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