BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fbVf0014
(389 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g61560.1 68418.m07725 protein kinase family protein contains ... 29 1.5
At4g38600.2 68417.m05463 HECT-domain-containing protein / ubiqui... 29 1.5
At4g38600.1 68417.m05464 HECT-domain-containing protein / ubiqui... 29 1.5
At3g47940.1 68416.m05227 DNAJ heat shock protein, putative simil... 28 1.9
At1g33880.1 68414.m04200 avirulence-responsive family protein / ... 28 2.6
At1g10300.1 68414.m01160 GTP-binding protein-related contains si... 28 2.6
At4g31160.1 68417.m04423 transducin family protein / WD-40 repea... 27 3.4
At3g24840.1 68416.m03116 SEC14 cytosolic factor, putative / phos... 27 3.4
At5g59650.1 68418.m07479 leucine-rich repeat protein kinase, put... 27 4.5
At5g56890.1 68418.m07099 protein kinase family protein contains ... 27 5.9
At4g04610.1 68417.m00674 5'-adenylylsulfate reductase (APR1) / P... 27 5.9
At3g30230.1 68416.m03820 myosin heavy chain-related similar to M... 27 5.9
At3g18290.1 68416.m02326 zinc finger protein-related weak alignm... 27 5.9
At2g47520.1 68415.m05931 AP2 domain-containing transcription fac... 27 5.9
At1g55860.1 68414.m06406 ubiquitin-protein ligase 1 (UPL1) nearl... 27 5.9
At5g13080.1 68418.m01499 WRKY family transcription factor WRKY D... 26 7.8
At3g42770.1 68416.m04468 F-box family protein contains F-box dom... 26 7.8
At1g79190.1 68414.m09233 expressed protein 26 7.8
>At5g61560.1 68418.m07725 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 796
Score = 28.7 bits (61), Expect = 1.5
Identities = 16/55 (29%), Positives = 27/55 (49%)
Frame = +2
Query: 134 PDNKRSKIEQMFELQHLKTKFPSAQKAQGAPQSSGKSHDEPRRHQNQRQSEKSAR 298
P + S+I + FEL+ LK + + QS + + NQR+SE++ R
Sbjct: 286 PTSSSSQIHKDFELEKLKIELRHIKGMYAVAQSEVIDASKKMQDLNQRRSEEATR 340
>At4g38600.2 68417.m05463 HECT-domain-containing protein /
ubiquitin-transferase family protein similar to
SP|Q14669Thyroid receptor interacting protein 12 (TRIP12)
{Homo sapiens}; contains Pfam profile PF00632:
HECT-domain (ubiquitin-transferase)
Length = 1794
Score = 28.7 bits (61), Expect = 1.5
Identities = 16/46 (34%), Positives = 25/46 (54%)
Frame = +2
Query: 206 QKAQGAPQSSGKSHDEPRRHQNQRQSEKSARGRLVESSMAVS*TYS 343
Q+ QGA S + E R + QRQ + +R R+++S+ V YS
Sbjct: 1356 QQQQGADGSGSTNEREMRIGRLQRQKVRVSRNRILDSAAKVMEMYS 1401
>At4g38600.1 68417.m05464 HECT-domain-containing protein /
ubiquitin-transferase family protein similar to
SP|Q14669Thyroid receptor interacting protein 12 (TRIP12)
{Homo sapiens}; contains Pfam profile PF00632:
HECT-domain (ubiquitin-transferase)
Length = 1888
Score = 28.7 bits (61), Expect = 1.5
Identities = 16/46 (34%), Positives = 25/46 (54%)
Frame = +2
Query: 206 QKAQGAPQSSGKSHDEPRRHQNQRQSEKSARGRLVESSMAVS*TYS 343
Q+ QGA S + E R + QRQ + +R R+++S+ V YS
Sbjct: 1447 QQQQGADGSGSTNEREMRIGRLQRQKVRVSRNRILDSAAKVMEMYS 1492
>At3g47940.1 68416.m05227 DNAJ heat shock protein, putative similar
to SP|O89114 DnaJ homolog subfamily B member 5 (Heat
shock protein Hsp40-3) {Mus musculus}; contains Pfam
profile PF00226: DnaJ domain
Length = 350
Score = 28.3 bits (60), Expect = 1.9
Identities = 15/57 (26%), Positives = 31/57 (54%), Gaps = 1/57 (1%)
Frame = +2
Query: 128 LLPDNKRSKIEQMFELQHLKT-KFPSAQKAQGAPQSSGKSHDEPRRHQNQRQSEKSA 295
+L D ++ +I ++ + LK+ K P++ ++ + SS S P HQ++ Q +A
Sbjct: 57 VLSDPQKRQIYDLYGEEGLKSGKIPNSSSSEASSSSSSSSSRYPHFHQHRPQHPPNA 113
>At1g33880.1 68414.m04200 avirulence-responsive family protein /
avirulence induced gene (AIG1) family protein similar to
AIG1 protein SP:P54120 [Arabidopsis thaliana]; contains
Pfam PF04548: AIG1 family;
Length = 234
Score = 27.9 bits (59), Expect = 2.6
Identities = 11/23 (47%), Positives = 14/23 (60%)
Frame = -2
Query: 145 FIIREKDDYFPICCPAALNAEMR 77
F +E +DYFP CCP L +R
Sbjct: 150 FEAKEFEDYFPECCPEFLMRVLR 172
>At1g10300.1 68414.m01160 GTP-binding protein-related contains
similarity to nucleolar GTP-binding protein 1 SP: Q9BZE4
from [Homo sapiens];
Length = 687
Score = 27.9 bits (59), Expect = 2.6
Identities = 14/49 (28%), Positives = 28/49 (57%)
Frame = +3
Query: 195 FPRLKKRKELLSHLGNLMTNQDGTKISGRARNRQEDDSSKVVWRYLELI 341
FPRLK+ + L +++ N+D K++ N ++ SK+ Y++L+
Sbjct: 98 FPRLKEIQPFYEDLLHVLYNKDHYKLALGQVNTAKNKISKIAMDYVKLL 146
>At4g31160.1 68417.m04423 transducin family protein / WD-40 repeat
family protein contains 2 WD-40 repeats (PF00400);
KIAA0800 protein, Homo sapiens GI:3882321 EMBL:AB018343
Length = 1846
Score = 27.5 bits (58), Expect = 3.4
Identities = 12/35 (34%), Positives = 19/35 (54%)
Frame = +2
Query: 38 LTKSRHDVFLEARSHFGVKRCWTTDGKIIVLLPDN 142
L +S+ +FLE HF R GK+ +L+ +N
Sbjct: 79 LLESQESLFLEENGHFSNARGSHNSGKLCILIREN 113
>At3g24840.1 68416.m03116 SEC14 cytosolic factor, putative /
phosphoglyceride transfer protein, putative similar to
phosphatidylinositol transfer-like protein IV
(GI:14486707) [Lotus japonicus]; similar to SEC14
CYTOSOLIC FACTOR (PHOSPHATIDYLINOSITOL/
PHOSPHATIDYLCHOLINE TRANSFER PROTEIN) GB:P46250 from
[Candida albicans] (Yeast (1996) 12(11), 1097-1105);
contains Pfam PF00650 : CRAL/TRIO domain; contains Pfam
PF03765 : CRAL/TRIO, N-terminus
Length = 579
Score = 27.5 bits (58), Expect = 3.4
Identities = 15/58 (25%), Positives = 27/58 (46%)
Frame = +2
Query: 143 KRSKIEQMFELQHLKTKFPSAQKAQGAPQSSGKSHDEPRRHQNQRQSEKSARGRLVES 316
+ ++ ++F L+H+ T S + S HD +R Q Q+E GR+ +S
Sbjct: 356 ENGEVAKLFSLRHVNTDMSSPDGGHVRERESHPEHD--KRAQLSNQAEAVGVGRMEQS 411
>At5g59650.1 68418.m07479 leucine-rich repeat protein kinase,
putative contains leucine rich repeat (LRR) domains,
Pfam:PF00560; contains protein kinase domain,
Pfam:PF00069
Length = 892
Score = 27.1 bits (57), Expect = 4.5
Identities = 17/55 (30%), Positives = 25/55 (45%)
Frame = +3
Query: 186 KPSFPRLKKRKELLSHLGNLMTNQDGTKISGRARNRQEDDSSKVVWRYLELIHRC 350
+P + + + + +G +T D TKI N D S+ VWR LEL C
Sbjct: 786 QPVIDQSRSKSHISQWVGFELTRGDITKIMDP--NLNGDYESRSVWRVLELAMSC 838
>At5g56890.1 68418.m07099 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 1113
Score = 26.6 bits (56), Expect = 5.9
Identities = 11/37 (29%), Positives = 21/37 (56%)
Frame = +3
Query: 258 DGTKISGRARNRQEDDSSKVVWRYLELIHRCNTKNLV 368
DGTK++ + R + S+ +E++ R + +NLV
Sbjct: 744 DGTKVAVKVLKRDDQQGSREFLAEVEMLSRLHHRNLV 780
>At4g04610.1 68417.m00674 5'-adenylylsulfate reductase (APR1) / PAPS
reductase homolog (PRH19) identical to
5'-adenylylsulfate reductase [Arabidopsis thaliana]
GI:2738756; identical to cDNA PAPS reductase homolog
(PRH19) GI:1710111
Length = 465
Score = 26.6 bits (56), Expect = 5.9
Identities = 11/31 (35%), Positives = 17/31 (54%)
Frame = +3
Query: 210 KRKELLSHLGNLMTNQDGTKISGRARNRQED 302
K KE H GN+ N D K++G +++ D
Sbjct: 318 KAKECGLHKGNVKENSDDAKVNGESKSAVAD 348
>At3g30230.1 68416.m03820 myosin heavy chain-related similar to
Myosin heavy chain, non-muscle (Zipper protein) (Myosin
II)(SP:Q99323) {Drosophila melanogaster}
Length = 527
Score = 26.6 bits (56), Expect = 5.9
Identities = 13/33 (39%), Positives = 18/33 (54%)
Frame = +2
Query: 227 QSSGKSHDEPRRHQNQRQSEKSARGRLVESSMA 325
+S+ + D+ RRH + ARGRL ES A
Sbjct: 333 KSNAELQDQYRRHDKLQDELSVARGRLSESKSA 365
>At3g18290.1 68416.m02326 zinc finger protein-related weak alignment
to Pfam profiles: PF00097 Zinc finger, C3HC4 type (RING
finger) (2 copies)
Length = 1254
Score = 26.6 bits (56), Expect = 5.9
Identities = 15/38 (39%), Positives = 21/38 (55%), Gaps = 2/38 (5%)
Frame = -1
Query: 182 DAEVQTSVQSYCVYYPGEGRLFSHL--LSSSA*RRNES 75
D V+ Q+Y + + GE LF HL L +SA +ES
Sbjct: 117 DIRVKNVAQTYSLEHKGESNLFDHLFELLNSATETDES 154
>At2g47520.1 68415.m05931 AP2 domain-containing transcription
factor, putative
Length = 171
Score = 26.6 bits (56), Expect = 5.9
Identities = 17/42 (40%), Positives = 21/42 (50%)
Frame = +1
Query: 205 SKSARSSSVIWEIS*RTKTAPKSAAEREIGKRTTRRK*YGGI 330
SKS S + +S R K P S +E GKR R+ Y GI
Sbjct: 13 SKSESEPSQLGSVSSRKKRKPVSVSEERDGKR-ERKNLYRGI 53
>At1g55860.1 68414.m06406 ubiquitin-protein ligase 1 (UPL1) nearly
identical to ubiquitin-protein ligase 1 [Arabidopsis
thaliana] GI:7108521; E3, HECT-domain protein family;
similar to GI:7108521, GB:AAF36454 from [Arabidopsis
thaliana]
Length = 3891
Score = 26.6 bits (56), Expect = 5.9
Identities = 10/40 (25%), Positives = 28/40 (70%)
Frame = +3
Query: 192 SFPRLKKRKELLSHLGNLMTNQDGTKISGRARNRQEDDSS 311
SFPR+K++++ ++L ++ ++ TK+ G+++ + + +S
Sbjct: 1961 SFPRVKRQEDGETNLISMEVDEPTTKVKGKSKVGEPEKAS 2000
>At5g13080.1 68418.m01499 WRKY family transcription factor WRKY DNA
binding protein - Solanum tuberosum, EMBL:AJ278507
Length = 145
Score = 26.2 bits (55), Expect = 7.8
Identities = 11/23 (47%), Positives = 14/23 (60%)
Frame = +2
Query: 221 APQSSGKSHDEPRRHQNQRQSEK 289
AP S KSH +P HQ + +S K
Sbjct: 11 APFLSLKSHSKPELHQGEEESSK 33
>At3g42770.1 68416.m04468 F-box family protein contains F-box domain
Pfam:PF00646
Length = 532
Score = 26.2 bits (55), Expect = 7.8
Identities = 19/60 (31%), Positives = 28/60 (46%)
Frame = +2
Query: 5 LKGSRVIISEFLTKSRHDVFLEARSHFGVKRCWTTDGKIIVLLPDNKRSKIEQMFELQHL 184
L+ + I F K+R + LE SHF +K + K+ V L + K+E EL L
Sbjct: 461 LEDFAIHIKSFRRKARSENELEHISHFSLKMEFLEVLKVYVALTMDDTKKVELTKELLKL 520
>At1g79190.1 68414.m09233 expressed protein
Length = 1274
Score = 26.2 bits (55), Expect = 7.8
Identities = 13/30 (43%), Positives = 17/30 (56%)
Frame = -2
Query: 358 LVLHL*ISSRYRHTTFDESSSCRFLALPLI 269
++L L S RYR T +SSC A PL+
Sbjct: 935 ILLELNRSKRYRRTVGSIASSCLIAATPLL 964
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,159,835
Number of Sequences: 28952
Number of extensions: 156519
Number of successful extensions: 553
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 540
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 553
length of database: 12,070,560
effective HSP length: 73
effective length of database: 9,957,064
effective search space used: 557595584
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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