BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= e96h0163 (710 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p... 161 9e-41 SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ... 153 2e-38 SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc... 136 2e-33 SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 134 9e-33 SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce... 107 1e-24 SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch... 55 1e-08 SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pom... 44 2e-05 SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe... 30 0.38 SPAC25B8.04c |||mitochondrial splicing suppressor |Schizosacchar... 29 0.87 SPAC22G7.06c |ura1||carbamoyl-phosphate synthase |Schizosaccharo... 27 2.0 SPAC2F7.03c |pom1||DYRK family protein kinase Pom1|Schizosacchar... 25 8.1 SPAC823.11 |||sphingosine-1-phosphate phosphatase |Schizosacchar... 25 8.1 >SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces pombe|chr 3|||Manual Length = 647 Score = 161 bits (391), Expect = 9e-41 Identities = 75/105 (71%), Positives = 87/105 (82%) Frame = +1 Query: 244 PQQQIFDAKRLIGRKFEDATVQADMKHWPFEVVSXGGKPKIKVAYKGEDKTFFPEEVSSM 423 P IFDAKRLIGRKF+D VQ+DMKHWPF+V+S GKP ++V YKGE KTF PEE+SSM Sbjct: 61 PHNTIFDAKRLIGRKFDDPEVQSDMKHWPFKVISKDGKPVLQVEYKGETKTFTPEEISSM 120 Query: 424 VLTKMKETAEAYLGKTVQNAVITVPAVLHDSQRQATKDAGTISGL 558 VL KM+ETAEAYLG V +AV+TVPA +DSQRQATKDAG I+GL Sbjct: 121 VLMKMRETAEAYLGGKVTDAVVTVPAYFNDSQRQATKDAGLIAGL 165 Score = 150 bits (364), Expect = 2e-37 Identities = 99/212 (46%), Positives = 116/212 (54%), Gaps = 11/212 (5%) Frame = +2 Query: 74 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNN 253 ++GIDLGTTYSCVG F + +VEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP+N Sbjct: 4 SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPHN 63 Query: 254 -----------KYSMPNVSSDVSSKMLLCKPT*STGLSRLSVXEANLRSR*HIRVKTKPF 400 K+ P V SD+ K K G L V + +TK F Sbjct: 64 TIFDAKRLIGRKFDDPEVQSDM--KHWPFKVISKDGKPVLQV---------EYKGETKTF 112 Query: 401 SPRKSVPWCXXXXXXXXXXXXXXXCRMQLSRFPRYFMTLKDKPQKMQVPSLGLNVLRIIN 580 +P + + P YF + + K GLNVLRIIN Sbjct: 113 TPEEISSMVLMKMRETAEAYLGGKVTDAVVTVPAYFNDSQRQATKDAGLIAGLNVLRIIN 172 Query: 581 EPTAAAIAYGLGQKGYWENEMYFIFDFGGGTF 676 EPTAAAIAYGL + E+ + IFD GGGTF Sbjct: 173 EPTAAAIAYGLDRSNQGESNV-LIFDLGGGTF 203 >SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces pombe|chr 1|||Manual Length = 644 Score = 153 bits (372), Expect = 2e-38 Identities = 71/105 (67%), Positives = 85/105 (80%) Frame = +1 Query: 244 PQQQIFDAKRLIGRKFEDATVQADMKHWPFEVVSXGGKPKIKVAYKGEDKTFFPEEVSSM 423 P IFDAKRLIGR+F D VQ+DMKHWPF+V+ GKP I+V +KGE KTF PEE+SSM Sbjct: 61 PHNTIFDAKRLIGRRFNDPEVQSDMKHWPFKVIEKDGKPLIQVEFKGETKTFTPEEISSM 120 Query: 424 VLTKMKETAEAYLGKTVQNAVITVPAVLHDSQRQATKDAGTISGL 558 VL KM+E+AEA+LG V +AV+TVPA +DSQRQATKDAG I+GL Sbjct: 121 VLLKMRESAEAFLGGKVTDAVVTVPAYFNDSQRQATKDAGLIAGL 165 Score = 149 bits (361), Expect = 4e-37 Identities = 97/212 (45%), Positives = 116/212 (54%), Gaps = 11/212 (5%) Frame = +2 Query: 74 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNN 253 ++GIDLGTTYSCVG F + +VEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP+N Sbjct: 4 SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPHN 63 Query: 254 -----------KYSMPNVSSDVSSKMLLCKPT*STGLSRLSVXEANLRSR*HIRVKTKPF 400 +++ P V SD+ K K G + V + +TK F Sbjct: 64 TIFDAKRLIGRRFNDPEVQSDM--KHWPFKVIEKDGKPLIQV---------EFKGETKTF 112 Query: 401 SPRKSVPWCXXXXXXXXXXXXXXXCRMQLSRFPRYFMTLKDKPQKMQVPSLGLNVLRIIN 580 +P + + P YF + + K GLNVLRIIN Sbjct: 113 TPEEISSMVLLKMRESAEAFLGGKVTDAVVTVPAYFNDSQRQATKDAGLIAGLNVLRIIN 172 Query: 581 EPTAAAIAYGLGQKGYWENEMYFIFDFGGGTF 676 EPTAAAIAYGL + E + IFD GGGTF Sbjct: 173 EPTAAAIAYGLDRSNQHETNV-LIFDLGGGTF 203 >SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces pombe|chr 2|||Manual Length = 613 Score = 136 bits (330), Expect = 2e-33 Identities = 64/105 (60%), Positives = 78/105 (74%) Frame = +1 Query: 244 PQQQIFDAKRLIGRKFEDATVQADMKHWPFEVVSXGGKPKIKVAYKGEDKTFFPEEVSSM 423 P+ +FDAKRLIGR++ED Q D+KHWPF+V+ G P I+V Y GE K F +E+S+M Sbjct: 65 PRNTVFDAKRLIGRRYEDPETQKDIKHWPFKVIDNNGIPTIEVNYLGEKKQFTAQEISAM 124 Query: 424 VLTKMKETAEAYLGKTVQNAVITVPAVLHDSQRQATKDAGTISGL 558 VLTKMKE +EA L K V+ AVITVPA DSQR ATKDAG I+GL Sbjct: 125 VLTKMKEISEAKLNKRVEKAVITVPAYFSDSQRAATKDAGAIAGL 169 Score = 131 bits (316), Expect = 1e-31 Identities = 88/212 (41%), Positives = 107/212 (50%), Gaps = 11/212 (5%) Frame = +2 Query: 74 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNN 253 A+GIDLGTTYSCV V++ VEII NDQG RTTPS+VAFT+TERL+GDAAKNQ AMNP N Sbjct: 8 AIGIDLGTTYSCVAVWETANVEIIPNDQGARTTPSFVAFTETERLVGDAAKNQAAMNPRN 67 Query: 254 -----------KYSMPNVSSDVSSKMLLCKPT*STGLSRLSVXEANLRSR*HIRVKTKPF 400 +Y P D+ K K + G+ + V + K F Sbjct: 68 TVFDAKRLIGRRYEDPETQKDI--KHWPFKVIDNNGIPTIEVNYLG---------EKKQF 116 Query: 401 SPRKSVPWCXXXXXXXXXXXXXXXCRMQLSRFPRYFMTLKDKPQKMQVPSLGLNVLRIIN 580 + ++ + P YF + K GLNVLRIIN Sbjct: 117 TAQEISAMVLTKMKEISEAKLNKRVEKAVITVPAYFSDSQRAATKDAGAIAGLNVLRIIN 176 Query: 581 EPTAAAIAYGLGQKGYWENEMYFIFDFGGGTF 676 EPTAAAIAYGL K + + IFD GGGTF Sbjct: 177 EPTAAAIAYGLDAKSD-KPKNVLIFDLGGGTF 207 >SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||Manual Length = 663 Score = 134 bits (325), Expect = 9e-33 Identities = 61/105 (58%), Positives = 83/105 (79%) Frame = +1 Query: 244 PQQQIFDAKRLIGRKFEDATVQADMKHWPFEVVSXGGKPKIKVAYKGEDKTFFPEEVSSM 423 P+ IFD KRLIGRKF++ T+ D+K +PF +V+ +P ++V G+ K F PEE+S+M Sbjct: 94 PENTIFDIKRLIGRKFDEKTMAKDIKSFPFHIVNDKNRPLVEVNVGGKKKKFTPEEISAM 153 Query: 424 VLTKMKETAEAYLGKTVQNAVITVPAVLHDSQRQATKDAGTISGL 558 +L+KMK+TAEAYLGK V +AV+TVPA +D+QRQATKDAGTI+GL Sbjct: 154 ILSKMKQTAEAYLGKPVTHAVVTVPAYFNDAQRQATKDAGTIAGL 198 Score = 132 bits (319), Expect = 5e-32 Identities = 81/204 (39%), Positives = 110/204 (53%), Gaps = 4/204 (1%) Frame = +2 Query: 77 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNNK 256 +GIDLGTTYSCV V ++G+VEIIANDQGNR TPSYVAFT+ ERL+G+AAKNQ NP N Sbjct: 38 IGIDLGTTYSCVAVMKNGRVEIIANDQGNRITPSYVAFTEDERLVGEAAKNQAPSNPEN- 96 Query: 257 YSMPNVSSDVSSKMLLCKPT*STGLSRLSVXEANLRSR*HIRV----KTKPFSPRKSVPW 424 ++ ++ + K + T + + N ++R + V K K F+P + Sbjct: 97 -TIFDIKRLIGRK--FDEKTMAKDIKSFPFHIVNDKNRPLVEVNVGGKKKKFTPEEISAM 153 Query: 425 CXXXXXXXXXXXXXXXCRMQLSRFPRYFMTLKDKPQKMQVPSLGLNVLRIINEPTAAAIA 604 + P YF + + K GLNV+RI+NEPTAAAIA Sbjct: 154 ILSKMKQTAEAYLGKPVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVIRIVNEPTAAAIA 213 Query: 605 YGLGQKGYWENEMYFIFDFGGGTF 676 YGL + + ++D GGGTF Sbjct: 214 YGLDKTD--TEKHIVVYDLGGGTF 235 >SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces pombe|chr 1|||Manual Length = 674 Score = 107 bits (258), Expect = 1e-24 Identities = 72/211 (34%), Positives = 106/211 (50%), Gaps = 7/211 (3%) Frame = +2 Query: 65 KAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFT-DTERLIGDAAKNQVAM 241 K P +GIDLGTT SC+ + + ++IAN +G RTTPS VAFT D ERL+G +AK Q + Sbjct: 49 KGPVIGIDLGTTTSCLAIMEGQTPKVIANAEGTRTTPSVVAFTKDGERLVGVSAKRQAVI 108 Query: 242 NPNNKYSMPNVSSDVSSKMLLCKPT*STGLSRLSVXEANLRSR*H------IRVKTKPFS 403 NP N + ++K L+ + + R + E + H + + K +S Sbjct: 109 NPENTF--------FATKRLIGRRFKEPEVQR-DIKEVPYKIVEHSNGDAWLEARGKTYS 159 Query: 404 PRKSVPWCXXXXXXXXXXXXXXXCRMQLSRFPRYFMTLKDKPQKMQVPSLGLNVLRIINE 583 P + + + + P YF + + K GLNVLR++NE Sbjct: 160 PSQIGGFILSKMRETASTYLGKDVKNAVVTVPAYFNDSQRQATKAAGAIAGLNVLRVVNE 219 Query: 584 PTAAAIAYGLGQKGYWENEMYFIFDFGGGTF 676 PTAAA+AYGL +K + + +FD GGGTF Sbjct: 220 PTAAALAYGLDKK---NDAIVAVFDLGGGTF 247 Score = 102 bits (244), Expect = 6e-23 Identities = 49/105 (46%), Positives = 68/105 (64%) Frame = +1 Query: 244 PQQQIFDAKRLIGRKFEDATVQADMKHWPFEVVSXGGKPKIKVAYKGEDKTFFPEEVSSM 423 P+ F KRLIGR+F++ VQ D+K P+++V + KT+ P ++ Sbjct: 110 PENTFFATKRLIGRRFKEPEVQRDIKEVPYKIVEHSNGD---AWLEARGKTYSPSQIGGF 166 Query: 424 VLTKMKETAEAYLGKTVQNAVITVPAVLHDSQRQATKDAGTISGL 558 +L+KM+ETA YLGK V+NAV+TVPA +DSQRQATK AG I+GL Sbjct: 167 ILSKMRETASTYLGKDVKNAVVTVPAYFNDSQRQATKAAGAIAGL 211 >SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Schizosaccharomyces pombe|chr 1|||Manual Length = 720 Score = 54.8 bits (126), Expect = 1e-08 Identities = 53/220 (24%), Positives = 93/220 (42%), Gaps = 15/220 (6%) Frame = +2 Query: 62 AKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAM 241 ++ VGID G + + + V ++ +++I N+ NR+TPS V++ + R +G+AAK+ A Sbjct: 3 SRTNVVGIDFGNSKTVIAVARNRAIDVIVNEVSNRSTPSLVSYGERSRFLGEAAKSAEAS 62 Query: 242 NPNN-----------KYSMPNVSSDVSSKMLLCKPT*STGLSRLSVXEANLRSR-*HIRV 385 N N Y P + D+ S + K T G V N + +I++ Sbjct: 63 NFRNTVGSLKRLAGRTYDDPEI-KDIESNFISAKLTEVDGFVGAKVQYLNEETAFSNIQL 121 Query: 386 KTKPFSPRKSVPWCXXXXXXXXXXXXXXXCRMQLSRFPRYFMTLKDKPQKMQVPSLGLNV 565 F+ K++ + P +F ++ + GLN Sbjct: 122 IAAYFTKIKAIAEAELIGSVSDV----------VISIPAWFTDIQRRALLEAANIAGLNP 171 Query: 566 LRIINEPTAAAIAYGLGQKGYWENE---MYFIFDFGGGTF 676 LR++N+ AAA+ YG+ + E E I DFG + Sbjct: 172 LRLMNDNAAAALTYGITKTDLPEPESPRRVAIVDFGHSNY 211 Score = 51.6 bits (118), Expect = 1e-07 Identities = 32/98 (32%), Positives = 51/98 (52%), Gaps = 1/98 (1%) Frame = +1 Query: 268 KRLIGRKFEDATVQ-ADMKHWPFEVVSXGGKPKIKVAYKGEDKTFFPEEVSSMVLTKMKE 444 KRL GR ++D ++ + ++ G KV Y E+ F ++ + TK+K Sbjct: 72 KRLAGRTYDDPEIKDIESNFISAKLTEVDGFVGAKVQYLNEETAFSNIQLIAAYFTKIKA 131 Query: 445 TAEAYLGKTVQNAVITVPAVLHDSQRQATKDAGTISGL 558 AEA L +V + VI++PA D QR+A +A I+GL Sbjct: 132 IAEAELIGSVSDVVISIPAWFTDIQRRALLEAANIAGL 169 >SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pombe|chr 1|||Manual Length = 566 Score = 44.0 bits (99), Expect = 2e-05 Identities = 18/59 (30%), Positives = 33/59 (55%) Frame = +2 Query: 77 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNN 253 +GI G S + + GK +++AN++GNR PS +++ + G A+ Q+ N +N Sbjct: 27 IGISFGNQNSSIAFNRDGKTDVLANEEGNRQIPSILSYHGDQEYHGVQARGQLVRNADN 85 Score = 39.9 bits (89), Expect = 4e-04 Identities = 20/58 (34%), Positives = 32/58 (55%) Frame = +1 Query: 385 EDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAVLHDSQRQATKDAGTISGL 558 ++K E S L ++ E+AE +LG V V++VP D+QR+A + A +GL Sbjct: 134 KEKILTAHEASVRHLRRLTESAEDFLGTKVNGCVMSVPVYFTDAQRKALESAANEAGL 191 >SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 848 Score = 29.9 bits (64), Expect = 0.38 Identities = 21/67 (31%), Positives = 31/67 (46%), Gaps = 1/67 (1%) Frame = +2 Query: 77 VGIDLGTTYSCVGVFQHG-KVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNN 253 + ID GT ++ + + G +EI+ R S VAF ER+ G A N P Sbjct: 25 LAIDYGTEWTKAALIKPGIPLEIVLTKDTRRKEQSAVAFKGNERIFGVDASNLATRFP-- 82 Query: 254 KYSMPNV 274 +S+ NV Sbjct: 83 AHSIRNV 89 Score = 26.2 bits (55), Expect = 4.6 Identities = 13/55 (23%), Positives = 27/55 (49%) Frame = +1 Query: 385 EDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAVLHDSQRQATKDAGTI 549 +++ + EE+ +M + AE + + + V+TVP ++ QR +A I Sbjct: 131 DEENYSLEEIIAMTMEHYISLAEEMAHEKITDLVLTVPPHFNELQRSILLEAARI 185 >SPAC25B8.04c |||mitochondrial splicing suppressor |Schizosaccharomyces pombe|chr 1|||Manual Length = 378 Score = 28.7 bits (61), Expect = 0.87 Identities = 12/32 (37%), Positives = 18/32 (56%) Frame = +3 Query: 69 HPQ*ESIWVPRTLALVSSSTGRWRSSPTTRAT 164 HP S W+P LVS+ W +SP+++ T Sbjct: 283 HPLYSSSWIPTLHDLVSTRCSVWLTSPSSQRT 314 >SPAC22G7.06c |ura1||carbamoyl-phosphate synthase |Schizosaccharomyces pombe|chr 1|||Manual Length = 2244 Score = 27.5 bits (58), Expect = 2.0 Identities = 14/45 (31%), Positives = 24/45 (53%) Frame = +2 Query: 173 PSYVAFTDTERLIGDAAKNQVAMNPNNKYSMPNVSSDVSSKMLLC 307 P +A T ER++ AAK A+N Y++ ++ D K++ C Sbjct: 1252 PQDLAPTTIERIVDAAAKIGEALNITGPYNIQFIAKDNEIKVIEC 1296 >SPAC2F7.03c |pom1||DYRK family protein kinase Pom1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1087 Score = 25.4 bits (53), Expect = 8.1 Identities = 17/66 (25%), Positives = 31/66 (46%) Frame = +1 Query: 55 QNGKSTRSRNRSGYHVLLRWCLPAREGGDHRQRPGQQDHSVLCCVHRHRASHRRCRQEPG 234 +NGK T +R +S + W P++E R G D + V R + + +EP Sbjct: 477 RNGKKTPTRTKSRMQQFINWFKPSKE----RSSNGNSDSASPPPVPRLSITRSQVSREP- 531 Query: 235 GDEPQQ 252 ++P++ Sbjct: 532 -EKPEE 536 >SPAC823.11 |||sphingosine-1-phosphate phosphatase |Schizosaccharomyces pombe|chr 1|||Manual Length = 411 Score = 25.4 bits (53), Expect = 8.1 Identities = 11/35 (31%), Positives = 19/35 (54%) Frame = -1 Query: 155 GRWR*SPPSRAGRHQRKSTWYPDRFLLRVLLPFCI 51 G W SP S + H ++++ +F +RVL C+ Sbjct: 276 GTWFASPESLSHLHDNLNSYFLLKFFVRVLFGVCM 310 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,048,249 Number of Sequences: 5004 Number of extensions: 64377 Number of successful extensions: 252 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 216 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 245 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 331187010 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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