BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= e96h0156 (644 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC13G1.12 |did2||vacuolar sorting protein Did2|Schizosaccharom... 115 7e-27 SPBC106.09 |cut4|apc1|anaphase-promoting complex subunit Apc1|Sc... 27 2.3 >SPBC13G1.12 |did2||vacuolar sorting protein Did2|Schizosaccharomyces pombe|chr 2|||Manual Length = 178 Score = 115 bits (276), Expect = 7e-27 Identities = 55/94 (58%), Positives = 76/94 (80%) Frame = +3 Query: 294 AIQKGNMEGARIHAENAIRQKNQALNYLRMSARVDAVSSRVQTALTTRKVTNSMAGVVKA 473 AI KGN E ARI+A NAIR++ ++LN L++S+R+DAVSSR+QTA+T R V+ +MAGVV+ Sbjct: 17 AITKGNSEIARIYASNAIRKQQESLNLLKLSSRIDAVSSRLQTAVTMRAVSGNMAGVVRG 76 Query: 474 MDAAMKFNELREISTLMDKFESQFEDLDVQSSYI 575 MD AMK L IS +MDKFE+QF+D++VQ+ Y+ Sbjct: 77 MDRAMKTMNLEMISQVMDKFEAQFDDVNVQTGYM 110 >SPBC106.09 |cut4|apc1|anaphase-promoting complex subunit Apc1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1458 Score = 27.1 bits (57), Expect = 2.3 Identities = 17/51 (33%), Positives = 29/51 (56%), Gaps = 3/51 (5%) Frame = +3 Query: 456 AGVVKAMDAAM---KFNELREISTLMDKFESQFEDLDVQSSYIGGMRCSQT 599 +G V+ ++A + ++ + +STL DK+ + DLD S Y +R SQT Sbjct: 1274 SGAVQKLEAPILLPPYDSISSVSTLGDKYWNLKIDLDNNSDYRELLRESQT 1324 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,220,515 Number of Sequences: 5004 Number of extensions: 37025 Number of successful extensions: 64 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 64 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 64 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 289756512 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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