Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--0408
(403 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g35530.1 68418.m04226 40S ribosomal protein S3 (RPS3C) 134 3e-32
At3g53870.1 68416.m05951 40S ribosomal protein S3 (RPS3B) riboso... 132 8e-32
At2g31610.1 68415.m03862 40S ribosomal protein S3 (RPS3A) 132 8e-32
At3g06190.2 68416.m00712 speckle-type POZ protein-related simila... 33 0.094
At3g06190.1 68416.m00711 speckle-type POZ protein-related simila... 32 0.16
At2g25150.1 68415.m03008 transferase family protein similar to 1... 31 0.29
At5g10290.1 68418.m01194 leucine-rich repeat family protein / pr... 28 2.7
At5g65240.1 68418.m08207 leucine-rich repeat family protein / pr... 27 3.5
At5g19000.1 68418.m02257 speckle-type POZ protein-related contai... 27 3.5
At2g05920.1 68415.m00642 subtilase family protein contains simil... 27 3.5
At1g22940.1 68414.m02867 thiamin biosynthesis protein, putative ... 26 8.2
At1g09660.2 68414.m01085 KH domain-containing quaking protein, p... 26 8.2
>At5g35530.1 68418.m04226 40S ribosomal protein S3 (RPS3C)
Length = 248
Score = 134 bits (323), Expect = 3e-32
Identities = 78/121 (64%), Positives = 91/121 (75%), Gaps = 1/121 (0%)
Frame = +2
Query: 32 ISKKRKFVGDGVFKAELN*FLTRELAEDGYSGVEVRVTPIRSEIIIMATRTQSVLGEKGR 211
ISKKRKFV DGVF AELN LTRELAEDGYSGVEVRVTP+R+EIII ATRTQ+VLGEKGR
Sbjct: 5 ISKKRKFVADGVFYAELNEVLTRELAEDGYSGVEVRVTPMRTEIIIRATRTQNVLGEKGR 64
Query: 212 RIRELTSVVQKRFNI-QSNL*NCMLKRWLLVVFALSPRPNL*DTSLSEVSLYRRACYGVL 388
RIRELTS+VQKRF Q ++ K + A++ +L L +++ RRACYGVL
Sbjct: 65 RIRELTSLVQKRFKFPQDSVELYAEKVANRGLCAIAQAESLRYKLLGGLAV-RRACYGVL 123
Query: 389 R 391
R
Sbjct: 124 R 124
Score = 71.3 bits (167), Expect = 2e-13
Identities = 35/49 (71%), Positives = 38/49 (77%)
Frame = +1
Query: 256 PEQSVELYAEKVATRGLCAIAQAESLRYKLIGGLAVPSCLLWCSPFIME 402
P+ SVELYAEKVA RGLCAIAQAESLRYKL+GGLAV F+ME
Sbjct: 80 PQDSVELYAEKVANRGLCAIAQAESLRYKLLGGLAVRRACYGVLRFVME 128
>At3g53870.1 68416.m05951 40S ribosomal protein S3 (RPS3B) ribosomal
protein S3a - Xenopus laevis, PIR:R3XL3A
Length = 249
Score = 132 bits (319), Expect = 8e-32
Identities = 76/121 (62%), Positives = 90/121 (74%), Gaps = 1/121 (0%)
Frame = +2
Query: 32 ISKKRKFVGDGVFKAELN*FLTRELAEDGYSGVEVRVTPIRSEIIIMATRTQSVLGEKGR 211
ISKKRKFV DGVF AELN LTRELAEDGYSGVEVRVTP+R+EIII ATRTQ+VLGEKGR
Sbjct: 5 ISKKRKFVADGVFYAELNEVLTRELAEDGYSGVEVRVTPMRTEIIIRATRTQNVLGEKGR 64
Query: 212 RIRELTSVVQKRFNIQSNL*NCMLKRW-LLVVFALSPRPNL*DTSLSEVSLYRRACYGVL 388
RIRELTS+VQKRF + ++ + A++ +L L +++ RRACYGVL
Sbjct: 65 RIRELTSLVQKRFKFPVDSVELYAEKVNNRGLCAIAQAESLRYKLLGGLAV-RRACYGVL 123
Query: 389 R 391
R
Sbjct: 124 R 124
Score = 67.3 bits (157), Expect = 4e-12
Identities = 34/49 (69%), Positives = 36/49 (73%)
Frame = +1
Query: 256 PEQSVELYAEKVATRGLCAIAQAESLRYKLIGGLAVPSCLLWCSPFIME 402
P SVELYAEKV RGLCAIAQAESLRYKL+GGLAV F+ME
Sbjct: 80 PVDSVELYAEKVNNRGLCAIAQAESLRYKLLGGLAVRRACYGVLRFVME 128
>At2g31610.1 68415.m03862 40S ribosomal protein S3 (RPS3A)
Length = 250
Score = 132 bits (319), Expect = 8e-32
Identities = 76/121 (62%), Positives = 90/121 (74%), Gaps = 1/121 (0%)
Frame = +2
Query: 32 ISKKRKFVGDGVFKAELN*FLTRELAEDGYSGVEVRVTPIRSEIIIMATRTQSVLGEKGR 211
ISKKRKFV DGVF AELN LTRELAEDGYSGVEVRVTP+R+EIII ATRTQ+VLGEKGR
Sbjct: 5 ISKKRKFVADGVFYAELNEVLTRELAEDGYSGVEVRVTPMRTEIIIRATRTQNVLGEKGR 64
Query: 212 RIRELTSVVQKRFNIQSNL*NCMLKRW-LLVVFALSPRPNL*DTSLSEVSLYRRACYGVL 388
RIRELTS+VQKRF + ++ + A++ +L L +++ RRACYGVL
Sbjct: 65 RIRELTSLVQKRFKFPVDSVELYAEKVNNRGLCAIAQAESLRYKLLGGLAV-RRACYGVL 123
Query: 389 R 391
R
Sbjct: 124 R 124
Score = 67.3 bits (157), Expect = 4e-12
Identities = 34/49 (69%), Positives = 36/49 (73%)
Frame = +1
Query: 256 PEQSVELYAEKVATRGLCAIAQAESLRYKLIGGLAVPSCLLWCSPFIME 402
P SVELYAEKV RGLCAIAQAESLRYKL+GGLAV F+ME
Sbjct: 80 PVDSVELYAEKVNNRGLCAIAQAESLRYKLLGGLAVRRACYGVLRFVME 128
>At3g06190.2 68416.m00712 speckle-type POZ protein-related similar
to SPOP (novel nuclear speckle-type protein)
(SP:O43791) [Homo sapiens]; contains Pfam PF00651 :
BTB/POZ domain; contains Pfam PF00917: MATH domain
Length = 295
Score = 32.7 bits (71), Expect = 0.094
Identities = 16/42 (38%), Positives = 24/42 (57%)
Frame = -1
Query: 265 IALDVESLLYYGSQLTDSASFLSEHTLCPGGHNNDLRADGSD 140
+ ++ ES S+L + + LSEH+L GH +L ADG D
Sbjct: 243 LEVEAESCPSLLSELLEYVARLSEHSLTSSGHRKELFADGCD 284
>At3g06190.1 68416.m00711 speckle-type POZ protein-related similar
to SPOP (novel nuclear speckle-type protein)
(SP:O43791) [Homo sapiens]; contains Pfam PF00651 :
BTB/POZ domain; contains Pfam PF00917: MATH domain
Length = 406
Score = 31.9 bits (69), Expect = 0.16
Identities = 14/30 (46%), Positives = 19/30 (63%)
Frame = -1
Query: 229 SQLTDSASFLSEHTLCPGGHNNDLRADGSD 140
S+L + + LSEH+L GH +L ADG D
Sbjct: 366 SELLEYVARLSEHSLTSSGHRKELFADGCD 395
>At2g25150.1 68415.m03008 transferase family protein similar to
10-deacetylbaccatin III-10-O-acetyl transferase
[gi:6746554], 2-debenzoyl-7,13-diacetylbaccatin
III-2-O-benzoyl transferase [gi:11559716] from Taxus
cuspidata; contains Pfam transferase family domain
PF00248; contains EST gb:R65039
Length = 461
Score = 31.1 bits (67), Expect = 0.29
Identities = 13/37 (35%), Positives = 21/37 (56%)
Frame = -1
Query: 160 LRADGSDPDFHAGVAVLGQLPSEELIEFRLENSISNK 50
L+ SDP GV V+ +LP + ++EF+ E + K
Sbjct: 418 LKPSKSDPSMEGGVKVIMKLPRDAMVEFKREMATMKK 454
>At5g10290.1 68418.m01194 leucine-rich repeat family protein /
protein kinase family protein contains Pfam domains
PF00560: Leucine Rich Repeat and PF00069: Protein kinase
domain
Length = 613
Score = 27.9 bits (59), Expect = 2.7
Identities = 13/39 (33%), Positives = 20/39 (51%)
Frame = -2
Query: 318 GDSAKTTSSHLFSIQFYRLLWMLNRFCTTEVSSRILRPF 202
GD+A + S+ +R L L FCTT+ ++ PF
Sbjct: 328 GDAAFQREVEMISVAVHRNLLRLIGFCTTQTERLLVYPF 366
>At5g65240.1 68418.m08207 leucine-rich repeat family protein /
protein kinase family protein contains Pfam domains
PF00560: Leucine Rich Repeat and PF00069: Protein kinase
domain
Length = 617
Score = 27.5 bits (58), Expect = 3.5
Identities = 13/39 (33%), Positives = 19/39 (48%)
Frame = -2
Query: 318 GDSAKTTSSHLFSIQFYRLLWMLNRFCTTEVSSRILRPF 202
GD A + S+ +R L L FCTT+ ++ PF
Sbjct: 332 GDEAFQREVEMISVAVHRNLLRLIGFCTTQTERLLVYPF 370
>At5g19000.1 68418.m02257 speckle-type POZ protein-related contains
Pfam PF00651 : BTB/POZ domain; contains Pfam PF00917:
MATH domain; similar to Speckle-type POZ protein
(SP:O43791) [Homo sapiens]
Length = 442
Score = 27.5 bits (58), Expect = 3.5
Identities = 11/30 (36%), Positives = 18/30 (60%)
Frame = -1
Query: 229 SQLTDSASFLSEHTLCPGGHNNDLRADGSD 140
++L + LSEH++ GH ++ ADG D
Sbjct: 402 TELLQYVARLSEHSVIVSGHRKEIFADGCD 431
>At2g05920.1 68415.m00642 subtilase family protein contains
similarity to cucumisin-like serine protease GI:3176874
from [Arabidopsis thaliana]
Length = 754
Score = 27.5 bits (58), Expect = 3.5
Identities = 20/48 (41%), Positives = 25/48 (52%), Gaps = 1/48 (2%)
Frame = -3
Query: 338 YLRDSAWAIAQRPRVATFSAYNSTDCSGC*I-ASVLRKSAHGFCVLSL 198
Y +A +A R RVAT+ ST C G I A++ R G VLSL
Sbjct: 229 YAAGTARGMATRARVATYKVCWSTGCFGSDILAAMDRAILDGVDVLSL 276
>At1g22940.1 68414.m02867 thiamin biosynthesis protein, putative
strong similarity to hydroxymethylpyrimidine
kinase/thiamin-phosphate pyrophosphorylase (BTH1)
GI:7488455 from [Brassica napus]
Length = 522
Score = 26.2 bits (55), Expect = 8.2
Identities = 17/45 (37%), Positives = 24/45 (53%)
Frame = -1
Query: 199 SEHTLCPGGHNNDLRADGSDPDFHAGVAVLGQLPSEELIEFRLEN 65
S H L P + L++ SD +F V G LPS E++E L+N
Sbjct: 78 SVHLLPPEFISEQLKSVLSDFEFD--VVKTGMLPSTEIVEVLLQN 120
>At1g09660.2 68414.m01085 KH domain-containing quaking protein,
putative similar to GB:AAC67357
Length = 264
Score = 26.2 bits (55), Expect = 8.2
Identities = 15/40 (37%), Positives = 24/40 (60%)
Frame = -1
Query: 121 VAVLGQLPSEELIEFRLENSISNKFSLL*NIVHGHDLVPN 2
V + +LP E++I RLE+++ SLL +VH + PN
Sbjct: 218 VLIEAELP-EDIINSRLEHAVHFLESLLKPMVHSYHKSPN 256
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,921,845
Number of Sequences: 28952
Number of extensions: 167721
Number of successful extensions: 434
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 420
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 431
length of database: 12,070,560
effective HSP length: 74
effective length of database: 9,928,112
effective search space used: 585758608
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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