BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 030623sawa_F03_e22_11.seq
         (1599 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) str...   190   3e-48
At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S...   188   9e-48
At1g21730.1 68414.m02720 kinesin-related protein (MKRP1) Similar...    31   1.6  
At2g35640.1 68415.m04371 hydroxyproline-rich glycoprotein family...    30   4.9  
At2g18470.1 68415.m02151 protein kinase family protein contains ...    29   6.5  
At1g70370.1 68414.m08095 BURP domain-containing protein / polyga...    29   6.5  
At4g08380.1 68417.m01384 proline-rich extensin-like family prote...    29   8.6  
At1g27740.1 68414.m03390 basic helix-loop-helix (bHLH) family pr...    29   8.6  

>At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) strong
           similarity to 60S ribosomal protein L1 GB:P49691
          Length = 406

 Score =  190 bits (462), Expect = 3e-48
 Identities = 92/172 (53%), Positives = 111/172 (64%), Gaps = 2/172 (1%)
 Frame = +2

Query: 128 MSLSVARPLVSVYSEKSEVVAGAS--LPLPFVFKAPIRPDLVNDVHVSMSKNARQPYCVS 301
           M+ + ARPLV++ +   ++    S  + LP V  AP+RPD+VN VH  +S N+RQPY VS
Sbjct: 1   MAAAAARPLVTIQTLDGDMSTDQSSTVVLPDVMTAPVRPDIVNFVHAQISNNSRQPYAVS 60

Query: 302 KEAGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWXXXXXXX 481
           K+AGHQTSAESWGTGRAV+RIPRV GGGTHR+GQ AFGNMCRGGRMFAPTK W       
Sbjct: 61  KKAGHQTSAESWGTGRAVSRIPRVPGGGTHRAGQAAFGNMCRGGRMFAPTKIWRRWHRRV 120

Query: 482 XXXXXXXXXXXXXXXXXXXXLVQARGHIVEKIPELPLVVSDNVXGXHKTKQA 637
                               LV ARGH +E +PE+PLVVSD+     KT  A
Sbjct: 121 NVNMKRHAIVSAIAATAVPALVMARGHKIENVPEMPLVVSDSAEAVEKTSAA 172


>At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S
           roibosomal protein L4, Arabidopsis thaliana,
           EMBL:CAA79104
          Length = 407

 Score =  188 bits (458), Expect = 9e-48
 Identities = 92/169 (54%), Positives = 108/169 (63%), Gaps = 2/169 (1%)
 Frame = +2

Query: 137 SVARPLVSVYSEKSEVVAGAS--LPLPFVFKAPIRPDLVNDVHVSMSKNARQPYCVSKEA 310
           + ARPLV+V     ++    S  + LP V  AP+RPD+VN VH  +S N+RQPY VSK+A
Sbjct: 5   AAARPLVTVQGLDGDMSTDQSTTVTLPDVMTAPVRPDIVNFVHAQISNNSRQPYAVSKKA 64

Query: 311 GHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWXXXXXXXXXX 490
           GHQTSAESWGTGRAV+RIPRV GGGTHR+GQ AFGNMCRGGRMFAPTK W          
Sbjct: 65  GHQTSAESWGTGRAVSRIPRVPGGGTHRAGQAAFGNMCRGGRMFAPTKIWRRWHRRVNVN 124

Query: 491 XXXXXXXXXXXXXXXXXLVQARGHIVEKIPELPLVVSDNVXGXHKTKQA 637
                            LV ARGH +E +PE+PLVVSD+     KT  A
Sbjct: 125 MKRHAIVSAIAATAVPALVMARGHKIENVPEMPLVVSDSAEAVEKTSAA 173


>At1g21730.1 68414.m02720 kinesin-related protein (MKRP1) Similar to
           gb|U06698 neuronal kinesin heavy chain from Homo sapiens
           and contains a PF|00225 Kinesin motor domain. EST
           gb|AA042507 comes from this gene; identical to cDNA
           MKRP1 mRNA for kinesin-related protein,  GI:16902291,
           kinesin-related protein [Arabidopsis thaliana]
           GI:16902292
          Length = 890

 Score = 31.5 bits (68), Expect = 1.6
 Identities = 16/37 (43%), Positives = 22/37 (59%), Gaps = 1/37 (2%)
 Frame = -1

Query: 258 WTSLTRS-GLMGALNTNGRGSEAPATTSLFSEYTDTK 151
           W  L +S G+ G L T+G  S+A  + S  S+YT TK
Sbjct: 549 WLKLKKSDGVAGTLPTDGNQSQASGSPSSSSKYTQTK 585


>At2g35640.1 68415.m04371 hydroxyproline-rich glycoprotein family
           protein contains proline-rich extensin domains,
           INTERPRO:IPR002965
          Length = 340

 Score = 29.9 bits (64), Expect = 4.9
 Identities = 14/49 (28%), Positives = 22/49 (44%)
 Frame = -1

Query: 471 CQRLHGLVGANIRPPRHMLPNAPCPDLWVPPPRTRGIRATARPVPQDSA 325
           CQ+  G V   +  P H +P      L  PPP++  +   + P P  S+
Sbjct: 169 CQQSLGFVAPMMAQPMHQIPTTIVLSLPPPPPQSLSLSLPSPPQPPPSS 217


>At2g18470.1 68415.m02151 protein kinase family protein contains
           Pfam PF00069: Protein kinase domain
          Length = 633

 Score = 29.5 bits (63), Expect = 6.5
 Identities = 14/40 (35%), Positives = 20/40 (50%)
 Frame = -3

Query: 577 NLLNNMPSSLNEGRYPGRCHGGGHGSTSLP*VHATVPAPP 458
           N  NN PS  ++G    R +GG +G    P    + P+PP
Sbjct: 66  NSSNNSPSPPSQGGGGERGNGGNNGGNDTPPSRGSPPSPP 105


>At1g70370.1 68414.m08095 BURP domain-containing protein /
           polygalacturonase, putative similar to polygalacturonase
           isoenzyme 1 beta subunit [Lycopersicon esculentum]
           GI:170480; contains Pfam profile PF03181: BURP domain
          Length = 626

 Score = 29.5 bits (63), Expect = 6.5
 Identities = 18/57 (31%), Positives = 25/57 (43%)
 Frame = -1

Query: 264 DTWTSLTRSGLMGALNTNGRGSEAPATTSLFSEYTDTKGRATDRLISLYQRSYSSKK 94
           DT+TS    G +   N N  G+   A    F+ Y D K    D   S Y +  +S+K
Sbjct: 278 DTFTSYGSDGNVPQNNFNNYGASGNAAVDTFANYRD-KANVGDDSFSSYAKDSNSEK 333


>At4g08380.1 68417.m01384 proline-rich extensin-like family protein
           contains proline-rich extensin domains,
           INTERPRO:IPR002965
          Length = 437

 Score = 29.1 bits (62), Expect = 8.6
 Identities = 10/19 (52%), Positives = 12/19 (63%)
 Frame = -1

Query: 432 PPRHMLPNAPCPDLWVPPP 376
           PP    P  PCPD++ PPP
Sbjct: 385 PPYAYSPPPPCPDVYKPPP 403


>At1g27740.1 68414.m03390 basic helix-loop-helix (bHLH) family
           protein contains Pfam profile: PF00010 helix-loop-helix
           DNA-binding domain
          Length = 258

 Score = 29.1 bits (62), Expect = 8.6
 Identities = 20/54 (37%), Positives = 26/54 (48%)
 Frame = -1

Query: 216 TNGRGSEAPATTSLFSEYTDTKGRATDRLISLYQRSYSSKKHYRRWQRPSLVPN 55
           +N    E  + TS+  +   TKG ATD   SLY R    K + R     +LVPN
Sbjct: 150 SNSSDDEKASVTSVKGKTRATKGTATDPQ-SLYARKRREKINERLKTLQNLVPN 202


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 20,069,554
Number of Sequences: 28952
Number of extensions: 334934
Number of successful extensions: 914
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 857
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 911
length of database: 12,070,560
effective HSP length: 84
effective length of database: 9,638,592
effective search space used: 4318089216
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)