KWMTBOMO00548

Protein

Length

1,690 amino acids

Protein family membership

None predicted.

Domains and repeats

Domain
10 - 163
CRAL-TRIO lipid binding domain (IPR001251)
1,237 - 1,422
Dbl homology (DH) domain (IPR000219)
1,425 - 1,551
PH domain-like (IPR011993)
Pleckstrin homology domain (IPR001849)
1,603 - 1,683
SH3 domain (IPR001452)
Repeat
166 - 285
Spectrin/alpha-actinin (IPR018159)
291 - 394
Spectrin/alpha-actinin (IPR018159)
544 - 646
Spectrin/alpha-actinin (IPR018159)
649 - 753
Spectrin/alpha-actinin (IPR018159)
759 - 875
Spectrin/alpha-actinin (IPR018159)
881 - 982
Spectrin/alpha-actinin (IPR018159)
1,120 - 1,206
Spectrin/alpha-actinin (IPR018159)
Repeat
289 - 391
Spectrin repeat (IPR002017)
880 - 980
Spectrin repeat (IPR002017)

1 200 400 600 800 1000 1200 1400 1690

Detailed signature matches

IPR001251

CRAL-TRIO lipid binding domain
1. SSF52087 (CRAL/TRIO...)
  
  19 - 163
  
  SUPERFAMILY
  SSF52087 (CRAL/TRIO domain)
2. G3DSA:3.40.52...
  
  14 - 158
  
  GENE3D
  G3DSA:3.40.525.10 (G3DSA:3.40.525.10)
3. PF13716 (CRAL_TRIO_2)
  
  29 - 155
  
  Pfam
  PF13716 (CRAL_TRIO_2)
4. SM00516 (sec14_4)
  
  13 - 152
  
  SMART
  SM00516 (sec14_4)
5. PS50191 (CRAL_TRIO)
  
  10 - 155
  
  PROSITE profiles
  PS50191 (CRAL_TRIO)
IPR000219

Dbl homology (DH) domain
1. SSF48065 (DBL homol...)
  
  1,244 - 1,422
  
  SUPERFAMILY
  SSF48065 (DBL homology domain (DH-domain))
2. PS50010 (DH_2)
  
  1,244 - 1,419
  
  PROSITE profiles
  PS50010 (DH_2)
3. SM00325 (RhoGEF_3)
  
  1,248 - 1,418
  
  SMART
  SM00325 (RhoGEF_3)
4. G3DSA:1.20.90...
  
  1,237 - 1,421
  
  GENE3D
  G3DSA:1.20.900.10 (G3DSA:1.20.900.10)
5. PF00621 (RhoGEF)
  
  1,248 - 1,415
  
  Pfam
  PF00621 (RhoGEF)
IPR011993

PH domain-like
1. G3DSA:2.30.29.30
  
  1,425 - 1,551
  
  GENE3D
  G3DSA:2.30.29.30 (G3DSA:2.30.29.30)
2. SSF50729 (PH domain...)
  
  1,429 - 1,547
  
  SUPERFAMILY
  SSF50729 (PH domain-like)
IPR001849

Pleckstrin homology domain
1. PS50003 (PH_DOMAIN)
  
  1,431 - 1,544
  
  PROSITE profiles
  PS50003 (PH_DOMAIN)
2. SM00233 (PH_update)
  
  1,438 - 1,546
  
  SMART
  SM00233 (PH_update)
IPR001452

SH3 domain
1. PS50002 (SH3)
  
  1,603 - 1,667
  
  PROSITE profiles
  PS50002 (SH3)
2. SSF50044 (SH3-domain)
  
  1,605 - 1,683
  
  SUPERFAMILY
  SSF50044 (SH3-domain)
IPR018159

Spectrin/alpha-actinin
1. SM00150 (SPEC_4)
  
  1,120 - 1,206
  
  SMART
  SM00150 (SPEC_4)
  
  291 - 394
  
  SMART
  SM00150 (SPEC_4)
  
  649 - 753
  
  SMART
  SM00150 (SPEC_4)
  
  759 - 875
  
  SMART
  SM00150 (SPEC_4)
  
  166 - 285
  
  SMART
  SM00150 (SPEC_4)
  
  544 - 646
  
  SMART
  SM00150 (SPEC_4)
  
  881 - 982
  
  SMART
  SM00150 (SPEC_4)
IPR002017

Spectrin repeat
1. PF00435 (Spectrin)
  
  289 - 391
  
  Pfam
  PF00435 (Spectrin)
  
  880 - 980
  
  Pfam
  PF00435 (Spectrin)

no IPR

Unintegrated signatures

Coil

217 - 237

COILS
Coil (Coil)

165 - 185

COILS
Coil (Coil)
G3DSA:1.20.58.60

1,152 - 1,198

GENE3D
G3DSA:1.20.58.60 (G3DSA:1.20.58.60)

875 - 980

GENE3D
G3DSA:1.20.58.60 (G3DSA:1.20.58.60)

286 - 393

GENE3D
G3DSA:1.20.58.60 (G3DSA:1.20.58.60)

1,113 - 1,139

GENE3D
G3DSA:1.20.58.60 (G3DSA:1.20.58.60)

541 - 646

GENE3D
G3DSA:1.20.58.60 (G3DSA:1.20.58.60)

683 - 762

GENE3D
G3DSA:1.20.58.60 (G3DSA:1.20.58.60)

163 - 285

GENE3D
G3DSA:1.20.58.60 (G3DSA:1.20.58.60)
G3DSA:2.30.30.40

1,594 - 1,661

GENE3D
G3DSA:2.30.30.40 (G3DSA:2.30.30.40)
SSF46966 (Spectrin ...)

256 - 393

SUPERFAMILY
SSF46966 (Spectrin repeat)

840 - 980

SUPERFAMILY
SSF46966 (Spectrin repeat)

1,084 - 1,196

SUPERFAMILY
SSF46966 (Spectrin repeat)

496 - 646

SUPERFAMILY
SSF46966 (Spectrin repeat)

710 - 821

SUPERFAMILY
SSF46966 (Spectrin repeat)
cd00160 (RhoGEF)

1,247 - 1,417

CDD
cd00160 (RhoGEF)
cd00170 (SEC14)

21 - 150

CDD
cd00170 (SEC14)
cd00176 (SPEC)

757 - 985

CDD
cd00176 (SPEC)

165 - 389

CDD
cd00176 (SPEC)

879 - 1,116

CDD
cd00176 (SPEC)
cd13240 (PH1_Kaliri...)

1,424 - 1,547

CDD
cd13240 (PH1_Kalirin_Trio_like)
mobidb-lite (disord...)

484 - 503

MobiDB Lite
mobidb-lite (disorder_prediction)

1,651 - 1,690

MobiDB Lite
mobidb-lite (disorder_prediction)

1,555 - 1,604

MobiDB Lite
mobidb-lite (disorder_prediction)

1,196 - 1,241

MobiDB Lite
mobidb-lite (disorder_prediction)

Residue annotation

phospholipid bindi...

31P

phospholipid binding pocket
31P, 33I, 35F, 73I, 75M, 83I, 87L, 91Q, 95P, 98V, 101A, 103V, 109F, 116T, 128M
CDD
cd00170 (SEC14)

33I

phospholipid binding pocket
31P, 33I, 35F, 73I, 75M, 83I, 87L, 91Q, 95P, 98V, 101A, 103V, 109F, 116T, 128M
CDD
cd00170 (SEC14)

35F

phospholipid binding pocket
31P, 33I, 35F, 73I, 75M, 83I, 87L, 91Q, 95P, 98V, 101A, 103V, 109F, 116T, 128M
CDD
cd00170 (SEC14)

73I

phospholipid binding pocket
31P, 33I, 35F, 73I, 75M, 83I, 87L, 91Q, 95P, 98V, 101A, 103V, 109F, 116T, 128M
CDD
cd00170 (SEC14)

75M

phospholipid binding pocket
31P, 33I, 35F, 73I, 75M, 83I, 87L, 91Q, 95P, 98V, 101A, 103V, 109F, 116T, 128M
CDD
cd00170 (SEC14)

83I

phospholipid binding pocket
31P, 33I, 35F, 73I, 75M, 83I, 87L, 91Q, 95P, 98V, 101A, 103V, 109F, 116T, 128M
CDD
cd00170 (SEC14)

87L

phospholipid binding pocket
31P, 33I, 35F, 73I, 75M, 83I, 87L, 91Q, 95P, 98V, 101A, 103V, 109F, 116T, 128M
CDD
cd00170 (SEC14)

91Q

phospholipid binding pocket
31P, 33I, 35F, 73I, 75M, 83I, 87L, 91Q, 95P, 98V, 101A, 103V, 109F, 116T, 128M
CDD
cd00170 (SEC14)

95P

phospholipid binding pocket
31P, 33I, 35F, 73I, 75M, 83I, 87L, 91Q, 95P, 98V, 101A, 103V, 109F, 116T, 128M
CDD
cd00170 (SEC14)

98V

phospholipid binding pocket
31P, 33I, 35F, 73I, 75M, 83I, 87L, 91Q, 95P, 98V, 101A, 103V, 109F, 116T, 128M
CDD
cd00170 (SEC14)

101A

phospholipid binding pocket
31P, 33I, 35F, 73I, 75M, 83I, 87L, 91Q, 95P, 98V, 101A, 103V, 109F, 116T, 128M
CDD
cd00170 (SEC14)

103V

phospholipid binding pocket
31P, 33I, 35F, 73I, 75M, 83I, 87L, 91Q, 95P, 98V, 101A, 103V, 109F, 116T, 128M
CDD
cd00170 (SEC14)

109F

phospholipid binding pocket
31P, 33I, 35F, 73I, 75M, 83I, 87L, 91Q, 95P, 98V, 101A, 103V, 109F, 116T, 128M
CDD
cd00170 (SEC14)

116T

phospholipid binding pocket
31P, 33I, 35F, 73I, 75M, 83I, 87L, 91Q, 95P, 98V, 101A, 103V, 109F, 116T, 128M
CDD
cd00170 (SEC14)

128M

phospholipid binding pocket
31P, 33I, 35F, 73I, 75M, 83I, 87L, 91Q, 95P, 98V, 101A, 103V, 109F, 116T, 128M
CDD
cd00170 (SEC14)
salt bridge cd00170

96S

salt bridge
96S, 127T
CDD
cd00170 (SEC14)

127T

salt bridge
96S, 127T
CDD
cd00170 (SEC14)
linker region cd00...

287C

linker region
287C, 288F, 289Q, 290L, 291R, 292L
CDD
cd00176 (SPEC)

288F

linker region
287C, 288F, 289Q, 290L, 291R, 292L
CDD
cd00176 (SPEC)

289Q

linker region
287C, 288F, 289Q, 290L, 291R, 292L
CDD
cd00176 (SPEC)

290L

linker region
287C, 288F, 289Q, 290L, 291R, 292L
CDD
cd00176 (SPEC)

291R

linker region
287C, 288F, 289Q, 290L, 291R, 292L
CDD
cd00176 (SPEC)

292L

linker region
287C, 288F, 289Q, 290L, 291R, 292L
CDD
cd00176 (SPEC)
linker region cd00...

877C

linker region
877C, 878V, 879Q, 880L, 881G, 882Q
CDD
cd00176 (SPEC)

878V

linker region
877C, 878V, 879Q, 880L, 881G, 882Q
CDD
cd00176 (SPEC)

879Q

linker region
877C, 878V, 879Q, 880L, 881G, 882Q
CDD
cd00176 (SPEC)

880L

linker region
877C, 878V, 879Q, 880L, 881G, 882Q
CDD
cd00176 (SPEC)

881G

linker region
877C, 878V, 879Q, 880L, 881G, 882Q
CDD
cd00176 (SPEC)

882Q

linker region
877C, 878V, 879Q, 880L, 881G, 882Q
CDD
cd00176 (SPEC)
linker region cd00...

984S

linker region
984S, 985L, 986N
CDD
cd00176 (SPEC)

985L

linker region
984S, 985L, 986N
CDD
cd00176 (SPEC)

986N

linker region
984S, 985L, 986N
CDD
cd00176 (SPEC)
GTPase interaction...

1,251E

GTPase interaction site
1,251E, 1,255T, 1,350V, 1,371A, 1,372A, 1,375I, 1,376K, 1,378V, 1,379Q, 1,382T, 1,383K, 1,386L, 1,387L, 1,390D, 1,413P, 1,417N
CDD
cd00160 (RhoGEF)

1,255T

GTPase interaction site
1,251E, 1,255T, 1,350V, 1,371A, 1,372A, 1,375I, 1,376K, 1,378V, 1,379Q, 1,382T, 1,383K, 1,386L, 1,387L, 1,390D, 1,413P, 1,417N
CDD
cd00160 (RhoGEF)

1,350V

GTPase interaction site
1,251E, 1,255T, 1,350V, 1,371A, 1,372A, 1,375I, 1,376K, 1,378V, 1,379Q, 1,382T, 1,383K, 1,386L, 1,387L, 1,390D, 1,413P, 1,417N
CDD
cd00160 (RhoGEF)

1,371A

GTPase interaction site
1,251E, 1,255T, 1,350V, 1,371A, 1,372A, 1,375I, 1,376K, 1,378V, 1,379Q, 1,382T, 1,383K, 1,386L, 1,387L, 1,390D, 1,413P, 1,417N
CDD
cd00160 (RhoGEF)

1,372A

GTPase interaction site
1,251E, 1,255T, 1,350V, 1,371A, 1,372A, 1,375I, 1,376K, 1,378V, 1,379Q, 1,382T, 1,383K, 1,386L, 1,387L, 1,390D, 1,413P, 1,417N
CDD
cd00160 (RhoGEF)

1,375I

GTPase interaction site
1,251E, 1,255T, 1,350V, 1,371A, 1,372A, 1,375I, 1,376K, 1,378V, 1,379Q, 1,382T, 1,383K, 1,386L, 1,387L, 1,390D, 1,413P, 1,417N
CDD
cd00160 (RhoGEF)

1,376K

GTPase interaction site
1,251E, 1,255T, 1,350V, 1,371A, 1,372A, 1,375I, 1,376K, 1,378V, 1,379Q, 1,382T, 1,383K, 1,386L, 1,387L, 1,390D, 1,413P, 1,417N
CDD
cd00160 (RhoGEF)

1,378V

GTPase interaction site
1,251E, 1,255T, 1,350V, 1,371A, 1,372A, 1,375I, 1,376K, 1,378V, 1,379Q, 1,382T, 1,383K, 1,386L, 1,387L, 1,390D, 1,413P, 1,417N
CDD
cd00160 (RhoGEF)

1,379Q

GTPase interaction site
1,251E, 1,255T, 1,350V, 1,371A, 1,372A, 1,375I, 1,376K, 1,378V, 1,379Q, 1,382T, 1,383K, 1,386L, 1,387L, 1,390D, 1,413P, 1,417N
CDD
cd00160 (RhoGEF)

1,382T

GTPase interaction site
1,251E, 1,255T, 1,350V, 1,371A, 1,372A, 1,375I, 1,376K, 1,378V, 1,379Q, 1,382T, 1,383K, 1,386L, 1,387L, 1,390D, 1,413P, 1,417N
CDD
cd00160 (RhoGEF)

1,383K

GTPase interaction site
1,251E, 1,255T, 1,350V, 1,371A, 1,372A, 1,375I, 1,376K, 1,378V, 1,379Q, 1,382T, 1,383K, 1,386L, 1,387L, 1,390D, 1,413P, 1,417N
CDD
cd00160 (RhoGEF)

1,386L

GTPase interaction site
1,251E, 1,255T, 1,350V, 1,371A, 1,372A, 1,375I, 1,376K, 1,378V, 1,379Q, 1,382T, 1,383K, 1,386L, 1,387L, 1,390D, 1,413P, 1,417N
CDD
cd00160 (RhoGEF)

1,387L

GTPase interaction site
1,251E, 1,255T, 1,350V, 1,371A, 1,372A, 1,375I, 1,376K, 1,378V, 1,379Q, 1,382T, 1,383K, 1,386L, 1,387L, 1,390D, 1,413P, 1,417N
CDD
cd00160 (RhoGEF)

1,390D

GTPase interaction site
1,251E, 1,255T, 1,350V, 1,371A, 1,372A, 1,375I, 1,376K, 1,378V, 1,379Q, 1,382T, 1,383K, 1,386L, 1,387L, 1,390D, 1,413P, 1,417N
CDD
cd00160 (RhoGEF)

1,413P

GTPase interaction site
1,251E, 1,255T, 1,350V, 1,371A, 1,372A, 1,375I, 1,376K, 1,378V, 1,379Q, 1,382T, 1,383K, 1,386L, 1,387L, 1,390D, 1,413P, 1,417N
CDD
cd00160 (RhoGEF)

1,417N

GTPase interaction site
1,251E, 1,255T, 1,350V, 1,371A, 1,372A, 1,375I, 1,376K, 1,378V, 1,379Q, 1,382T, 1,383K, 1,386L, 1,387L, 1,390D, 1,413P, 1,417N
CDD
cd00160 (RhoGEF)
Rac1 binding site ...

1,441Q

Rac1 binding site
1,441Q, 1,481A, 1,483Y
CDD
cd13240 (PH1_Kalirin_Trio_like)

1,481A

Rac1 binding site
1,441Q, 1,481A, 1,483Y
CDD
cd13240 (PH1_Kalirin_Trio_like)

1,483Y

Rac1 binding site
1,441Q, 1,481A, 1,483Y
CDD
cd13240 (PH1_Kalirin_Trio_like)

GO term prediction

Biological Process

GO:0035023 regulation of Rho protein signal transduction

Molecular Function

GO:0005089 Rho guanyl-nucleotide exchange factor activity
GO:0005515 protein binding

Cellular Component