Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA001835-TA|BGIBMGA001835-PA|IPR001159|Double-stranded RNA
binding, IPR000999|Ribonuclease III
(152 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g01040.1 68414.m00004 DEAD/DEAH box helicase carpel factory /... 55 2e-08
At3g03300.1 68416.m00327 DEAD/DEAH box helicase carpel factory-r... 46 8e-06
At3g43920.1 68416.m04701 ribonuclease III family protein similar... 45 3e-05
At5g20320.1 68418.m02418 DEAD/DEAH box helicase, putative simila... 43 8e-05
At3g20420.1 68416.m02586 ribonuclease III family protein similar... 40 0.001
At4g15417.1 68417.m02358 ribonuclease III family protein similar... 34 0.048
At5g45150.1 68418.m05543 ribonuclease III family protein similar... 32 0.15
At3g59030.1 68416.m06579 transparent testa 12 protein (TT12) / m... 27 5.5
At2g40760.1 68415.m05028 rhodanese-like domain-containing protei... 26 9.6
At1g20800.1 68414.m02605 F-box family protein contains Pfam:PF00... 26 9.6
>At1g01040.1 68414.m00004 DEAD/DEAH box helicase carpel factory / CAF
identical to RNA helicase/RNAseIII CAF protein
GB:AAF03534 GI:6102610 from [Arabidopsis thaliana]
Length = 1909
Score = 55.2 bits (127), Expect = 2e-08
Identities = 40/128 (31%), Positives = 56/128 (43%), Gaps = 2/128 (1%)
Query: 19 DVEVPKALGDLFESVAGAIFLDSGMSLGAVWKSYGRLMGRELEAFSAAAPKSPVRELLEA 78
D + PK LGD+ ES+AGAIFLDSG A WK + L+ + P PVREL E
Sbjct: 1684 DCKAPKVLGDIVESIAGAIFLDSGKDTTAAWKVFQPLL--QPMVTPETLPMHPVRELQER 1741
Query: 79 EPDTAKFGKPERLADGRRVRVCVEXXXXXXXXXXXXNYRIAKGTAARCALRHLRRGRCSA 138
A+ + + G V V ++A+ AAR AL L+ +
Sbjct: 1742 CQQQAEGLEYKASRSGNTATVEVFIDGVQVGVAQNPQKKMAQKLAARNALAALKEKEIAE 1801
Query: 139 RPPPHTHS 146
H ++
Sbjct: 1802 SKEKHINN 1809
>At3g03300.1 68416.m00327 DEAD/DEAH box helicase carpel
factory-related similar to RNA helicase GB:AAF03534
Length = 1317
Score = 46.4 bits (105), Expect = 8e-06
Identities = 26/59 (44%), Positives = 34/59 (57%), Gaps = 2/59 (3%)
Query: 19 DVEVPKALGDLFESVAGAIFLDSGMSLGAVWKSYGRLMGRELEAFSAAAPKSPVRELLE 77
D+ PK LGD+ ES+AGAIF+DSG + V+ S L+G + PVREL E
Sbjct: 1202 DISFPKVLGDVIESLAGAIFVDSGYNKEVVFASIKPLLGCMIT--PETVKLHPVRELTE 1258
>At3g43920.1 68416.m04701 ribonuclease III family protein similar to
RNA helicase/RNAseIII CAF protein [Arabidopsis thaliana]
GI:6102610; contains Pfam profiles PF02170: PAZ domain,
PF00636: RNase3 domain
Length = 1531
Score = 44.8 bits (101), Expect = 3e-05
Identities = 25/83 (30%), Positives = 43/83 (51%), Gaps = 2/83 (2%)
Query: 20 VEVPKALGDLFESVAGAIFLDSGMSLGAVWKSYGRLMGRELEAFSAAAPKSPVRELLEAE 79
++ PKALGD+ ES+AGA+ +D+ + L VW+ + L+ + P P REL E
Sbjct: 1269 IQGPKALGDVVESIAGALLIDTRLDLDQVWRVFEPLLSPLVTPDKLQLP--PYRELNELC 1326
Query: 80 PDTAKFGKPERLADGRRVRVCVE 102
F + + DG + + ++
Sbjct: 1327 DSLGYFFRVKCSNDGVKAQATIQ 1349
>At5g20320.1 68418.m02418 DEAD/DEAH box helicase, putative similar to
CAF protein [Arabidopsis thaliana] GI:6102610; contains
Pfam profiles PF00270: DEAD/DEAH box helicase, PF00271:
Helicase conserved C-terminal domain, PF03368: Domain of
unknown function, PF00636: RNase3 domain, PF00035:
Double-stranded RNA binding motif
Length = 1676
Score = 43.2 bits (97), Expect = 8e-05
Identities = 24/62 (38%), Positives = 32/62 (51%), Gaps = 2/62 (3%)
Query: 16 QAEDVEVPKALGDLFESVAGAIFLDSGMSLGAVWKSYGRLMGRELEAFSAAAPKSPVREL 75
Q+E PK LGDL ES GA+FLD G +L VW + + SP++EL
Sbjct: 1384 QSEGPRCPKVLGDLVESCLGALFLDCGFNLNHVWTMMLSFLDPVKNLSNLQI--SPIKEL 1441
Query: 76 LE 77
+E
Sbjct: 1442 IE 1443
>At3g20420.1 68416.m02586 ribonuclease III family protein similar to
CAF protein (RNA helicase/RNAseIII) [Arabidopsis
thaliana] GI:6102610; contains Pfam profiles: PF00636
RNase3 domain, PF00035 Double-stranded RNA binding motif
Length = 391
Score = 39.5 bits (88), Expect = 0.001
Identities = 31/112 (27%), Positives = 45/112 (40%)
Query: 20 VEVPKALGDLFESVAGAIFLDSGMSLGAVWKSYGRLMGRELEAFSAAAPKSPVRELLEAE 79
V+ PK L DLFES+AGA+++D L +W + L+ + PV L +
Sbjct: 181 VKAPKVLADLFESLAGAVYVDVNFDLQRLWVIFRGLLEPIVTLDDLQKQPQPVSMLFKLC 240
Query: 80 PDTAKFGKPERLADGRRVRVCVEXXXXXXXXXXXXNYRIAKGTAARCALRHL 131
K + DG + N IA+ AA+ ALR L
Sbjct: 241 HKHKKRIDIKNWKDGNVSIAVIYLDDELLASGRAENKDIARLIAAKEALRKL 292
>At4g15417.1 68417.m02358 ribonuclease III family protein similar to
CAF protein (RNA helicase/RNAseIII) [Arabidopsis
thaliana] GI:6102610; contains Pfam profile PF00636
RNase3 domain
Length = 213
Score = 33.9 bits (74), Expect = 0.048
Identities = 16/31 (51%), Positives = 20/31 (64%), Gaps = 1/31 (3%)
Query: 20 VEVPKALGDLFESVAGAIFLDSGMSLGAVWK 50
++VPK L D+ ES GAIF+D S VWK
Sbjct: 149 LKVPKVLADIVESTIGAIFMDCN-STETVWK 178
>At5g45150.1 68418.m05543 ribonuclease III family protein similar to
CAF protein (RNA helicase/RNAseIII) [Arabidopsis
thaliana] GI:6102610; contains Pfam profiles PF00035:
Double-stranded RNA binding motif, PF00636 RNase3 domain
Length = 957
Score = 32.3 bits (70), Expect = 0.15
Identities = 12/37 (32%), Positives = 21/37 (56%)
Query: 20 VEVPKALGDLFESVAGAIFLDSGMSLGAVWKSYGRLM 56
V+ PK L D ES+A +F+D + +W+ + L+
Sbjct: 120 VKAPKILADTLESIAATVFIDVNYDVKRLWEIFRSLL 156
>At3g59030.1 68416.m06579 transparent testa 12 protein (TT12) /
multidrug transporter-like protein similar to ripening
regulated protein DDTFR18 [Lycopersicon esculentum]
GI:12231296, putative multidrug efflux protein NorM -
Vibrio parahaemolyticus, EMBL:AB010463; contains Pfam
profile PF01554: Uncharacterized membrane protein
family; identical to cDNA multidrug transporter-like
protein (tt12) GI:13624642, SP|Q9LYT3 TRANSPARENT TESTA
12 protein {Arabidopsis thaliana}, multidrug
transporter-like protein [Arabidopsis thaliana]
GI:13624643
Length = 507
Score = 27.1 bits (57), Expect = 5.5
Identities = 15/39 (38%), Positives = 25/39 (64%), Gaps = 6/39 (15%)
Query: 19 DVEVPKALGDLFESVAGAIFLD------SGMSLGAVWKS 51
D EV A+ DLF +A +IFL+ SG+++G+ W++
Sbjct: 387 DAEVIAAVSDLFPLLAVSIFLNGIQPILSGVAIGSGWQA 425
>At2g40760.1 68415.m05028 rhodanese-like domain-containing protein
contains rhodanese-like domain PF00581
Length = 474
Score = 26.2 bits (55), Expect = 9.6
Identities = 8/12 (66%), Positives = 11/12 (91%)
Query: 137 SARPPPHTHSPY 148
S+ PPPH+HSP+
Sbjct: 20 SSPPPPHSHSPF 31
>At1g20800.1 68414.m02605 F-box family protein contains Pfam:PF00646
F-box domain
Length = 492
Score = 26.2 bits (55), Expect = 9.6
Identities = 25/97 (25%), Positives = 48/97 (49%), Gaps = 13/97 (13%)
Query: 13 EMEQAEDVEVPKALGD------LFESVAGAIFLDSGMSLGA--VWKSYGRLMGRELEAFS 64
E+++ EDV++ + D S++ + L SG+S A ++K G+++G+ L
Sbjct: 95 ELDEKEDVDMMTMVNDDDDDDKRVSSLSKVMRLISGISPYAQSLFKKKGKILGKRL---- 150
Query: 65 AAAPKSPVRELLEAEPDTAKFGKPERLADGRRVRVCV 101
+ ++ EP+++KF ER+ RRV V
Sbjct: 151 -MIEEETESNMMMDEPNSSKFLDVERINKRRRVNPSV 186
Database: arabidopsis
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.320 0.135 0.409
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,517,785
Number of Sequences: 28952
Number of extensions: 114952
Number of successful extensions: 262
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 253
Number of HSP's gapped (non-prelim): 11
length of query: 152
length of database: 12,070,560
effective HSP length: 75
effective length of query: 77
effective length of database: 9,899,160
effective search space: 762235320
effective search space used: 762235320
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.8 bits)
S2: 55 (26.2 bits)
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