GeneModel BLAST Result against arabidopsis

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA001834-TA|BGIBMGA001834-PA|IPR000999|Ribonuclease III
         (157 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g01040.1 68414.m00004 DEAD/DEAH box helicase carpel factory /...   115   1e-26
At3g03300.1 68416.m00327 DEAD/DEAH box helicase carpel factory-r...   113   5e-26
At3g43920.1 68416.m04701 ribonuclease III family protein similar...    90   6e-19
At5g20320.1 68418.m02418 DEAD/DEAH box helicase, putative simila...    86   9e-18
At4g15417.1 68417.m02358 ribonuclease III family protein similar...    78   3e-15
At3g20420.1 68416.m02586 ribonuclease III family protein similar...    75   3e-14
At5g45150.1 68418.m05543 ribonuclease III family protein similar...    60   9e-10
At5g53280.1 68418.m06622 expressed protein                             33   0.12 
At4g01800.1 68417.m00237 preprotein translocase secA subunit, pu...    28   3.3  
At1g30270.1 68414.m03702 CBL-interacting protein kinase 23 (CIPK...    28   3.3  

>At1g01040.1 68414.m00004 DEAD/DEAH box helicase carpel factory / CAF
            identical to RNA helicase/RNAseIII CAF protein
            GB:AAF03534 GI:6102610 from [Arabidopsis thaliana]
          Length = 1909

 Score =  115 bits (277), Expect = 1e-26
 Identities = 56/112 (50%), Positives = 80/112 (71%), Gaps = 2/112 (1%)

Query: 15   LERVLQYRFRDRSLLLQAMTHASHHRNVLTDCYQRLEFLGDAILDYLITRHLYEDKRCHS 74
            LER L+Y F+++ LL++A+THAS   + ++ CYQRLEF+GDA+LD+LITRHL+       
Sbjct: 1562 LERALKYEFKEKGLLVEAITHASRPSSGVS-CYQRLEFVGDAVLDHLITRHLFFTYTSLP 1620

Query: 75   PGALTDLRSALVNNTIFATLAARHGFHKYFRHMSPGLNEVLKKYVK-IQEEN 125
            PG LTDLR+A VNN  FA +A +H  H Y RH S  L + ++++VK +Q E+
Sbjct: 1621 PGRLTDLRAAAVNNENFARVAVKHKLHLYLRHGSSALEKQIREFVKEVQTES 1672



 Score = 49.2 bits (112), Expect = 1e-06
 Identities = 30/92 (32%), Positives = 47/92 (51%), Gaps = 4/92 (4%)

Query: 14   QLERVLQYRFRDRSLLLQAMTHASHHRNVLTDCYQRLEFLGDAILDYLITRHLYEDKRCH 73
            QL+ ++ Y     S +L+A+T AS      T CY+R E LGDA L ++++R L+      
Sbjct: 1345 QLKNLISYPI-PTSKILEALTAASCQE---TFCYERAELLGDAYLKWVVSRFLFLKYPQK 1400

Query: 74   SPGALTDLRSALVNNTIFATLAARHGFHKYFR 105
              G LT +R  +V+N +    A   G   Y +
Sbjct: 1401 HEGQLTRMRQQMVSNMVLYQFALVKGLQSYIQ 1432


>At3g03300.1 68416.m00327 DEAD/DEAH box helicase carpel
            factory-related similar to RNA helicase GB:AAF03534
          Length = 1317

 Score =  113 bits (272), Expect = 5e-26
 Identities = 52/111 (46%), Positives = 76/111 (68%)

Query: 15   LERVLQYRFRDRSLLLQAMTHASHHRNVLTDCYQRLEFLGDAILDYLITRHLYEDKRCHS 74
            +E +L Y F D+SLL++A+TH S+    +  CYQRLEFLGD++LDYLIT+HLY+   C S
Sbjct: 1081 MESLLNYSFEDKSLLVEALTHGSYMMPEIPRCYQRLEFLGDSVLDYLITKHLYDKYPCLS 1140

Query: 75   PGALTDLRSALVNNTIFATLAARHGFHKYFRHMSPGLNEVLKKYVKIQEEN 125
            PG LTD+RSA VNN  +A +A +   HK+  + S  L++ + + V   E++
Sbjct: 1141 PGLLTDMRSASVNNECYALVAVKANLHKHILYASHHLHKHISRTVSEFEQS 1191


>At3g43920.1 68416.m04701 ribonuclease III family protein similar to
            RNA helicase/RNAseIII CAF protein [Arabidopsis thaliana]
            GI:6102610; contains Pfam profiles PF02170: PAZ domain,
            PF00636: RNase3 domain
          Length = 1531

 Score = 90.2 bits (214), Expect = 6e-19
 Identities = 48/119 (40%), Positives = 72/119 (60%), Gaps = 6/119 (5%)

Query: 14   QLERVLQYRFRDRSLLLQAMTHASHHRNVLTDCYQRLEFLGDAILDYLITRHLYEDKRCH 73
            +LER +Q+ F  + LL +A+TH+S   +     Y+RLEFLGD++LD+LITRHL+      
Sbjct: 1151 ELERKIQHEFSAKFLLKEAITHSSLRESY---SYERLEFLGDSVLDFLITRHLFNTYEQT 1207

Query: 74   SPGALTDLRSALVNNTIFATLAARHGFHKYFRHMSPGLNEVLKKYV---KIQEENGHSI 129
             PG +TDLRSA VNN  FA +A ++  H + +  +  L   +  Y+   +  +E G SI
Sbjct: 1208 GPGEMTDLRSACVNNENFAQVAVKNNLHTHLQRCATVLETQINDYLMSFQKPDETGRSI 1266



 Score = 35.1 bits (77), Expect = 0.022
 Identities = 17/59 (28%), Positives = 30/59 (50%)

Query: 48   QRLEFLGDAILDYLITRHLYEDKRCHSPGALTDLRSALVNNTIFATLAARHGFHKYFRH 106
            +RLE LGD++L Y+ + HL+        G L+  R ++++N+    L        Y R+
Sbjct: 973  ERLELLGDSVLKYVASCHLFLKYPDKDEGQLSRQRQSIISNSNLHRLTTSRKLQGYIRN 1031


>At5g20320.1 68418.m02418 DEAD/DEAH box helicase, putative similar to
            CAF protein [Arabidopsis thaliana] GI:6102610; contains
            Pfam profiles PF00270: DEAD/DEAH box helicase, PF00271:
            Helicase conserved C-terminal domain, PF03368: Domain of
            unknown function, PF00636: RNase3 domain, PF00035:
            Double-stranded RNA binding motif
          Length = 1676

 Score = 86.2 bits (204), Expect = 9e-18
 Identities = 47/106 (44%), Positives = 63/106 (59%), Gaps = 1/106 (0%)

Query: 13   EQLERVLQYRFRDRSLLLQAMTHASHHRNVLTDCYQRLEFLGDAILDYLITRHLYEDKRC 72
            E LE  L Y+F  + LL+QA  H S++R+    CYQRLEFLGDA+LDYL+T + +     
Sbjct: 1267 ETLENQLDYKFLHKGLLVQAFIHPSYNRHG-GGCYQRLEFLGDAVLDYLMTSYFFTVFPK 1325

Query: 73   HSPGALTDLRSALVNNTIFATLAARHGFHKYFRHMSPGLNEVLKKY 118
              PG LTDLRS  VNN   A +A      ++    S  L+EV++ Y
Sbjct: 1326 LKPGQLTDLRSLSVNNEALANVAVSFSLKRFLFCESIYLHEVIEDY 1371



 Score = 34.3 bits (75), Expect = 0.038
 Identities = 20/55 (36%), Positives = 29/55 (52%), Gaps = 3/55 (5%)

Query: 29   LLQAMTHASHHRNVLTDCYQRLEFLGDAILDYLITRHLYEDKRCHSPGALTDLRS 83
            +L+A+T    H  +     +RLE LGDA L + ++RHL+        G LT  RS
Sbjct: 1094 VLEALTTEKCHERL---SLERLEVLGDAFLKFAVSRHLFLHHDSLDEGELTRRRS 1145


>At4g15417.1 68417.m02358 ribonuclease III family protein similar to
           CAF protein (RNA helicase/RNAseIII) [Arabidopsis
           thaliana] GI:6102610; contains Pfam profile PF00636
           RNase3 domain
          Length = 213

 Score = 77.8 bits (183), Expect = 3e-15
 Identities = 43/117 (36%), Positives = 68/117 (58%), Gaps = 1/117 (0%)

Query: 8   ELHGYEQLERVLQYRFRDRSLLLQAMTHASHHRNVLTDCYQRLEFLGDAILDYLITRHLY 67
           +L   E LE++L Y+F+D+SLLL+A T AS+  +  ++ Y+ LE LGD+IL+  I     
Sbjct: 25  QLMEIESLEKILNYKFKDKSLLLKAFTDASYVDDK-SESYELLELLGDSILNMGIIYDFI 83

Query: 68  EDKRCHSPGALTDLRSALVNNTIFATLAARHGFHKYFRHMSPGLNEVLKKYVKIQEE 124
           +     +PG LT LR+  V+    A +A  H  + Y RH  P L E + ++V+  E+
Sbjct: 84  KLYPKEAPGPLTKLRAVNVDTEKLARVAVNHQLYSYLRHKKPLLEEQILEFVEAMEK 140


>At3g20420.1 68416.m02586 ribonuclease III family protein similar to
           CAF protein (RNA helicase/RNAseIII) [Arabidopsis
           thaliana] GI:6102610; contains Pfam profiles: PF00636
           RNase3 domain, PF00035 Double-stranded RNA binding motif
          Length = 391

 Score = 74.5 bits (175), Expect = 3e-14
 Identities = 43/129 (33%), Positives = 75/129 (58%), Gaps = 8/129 (6%)

Query: 5   VERELHGYEQLERVLQYRFRDRSLLLQAMTHASHHRNVLTD--CYQRLEFLGDAILDYLI 62
           V  E+   E +E++L Y+F ++SLL +A+TH S      TD   Y+RLEF+GD+ +   I
Sbjct: 53  VSSEMESMEAVEKILNYKFSNKSLLKEAITHTS-----CTDFPSYERLEFIGDSAIGLAI 107

Query: 63  TRHLYEDKRCHSPGALTDLRSALVNNTIFATLAARHGFHKYFRHMSPGLNEVLKKYVK-I 121
           + +LY       P  L+ LR+A V+    A ++  HG + + R  +P L+E +K++ + +
Sbjct: 108 SNYLYLTYPSLEPHDLSLLRAANVSTEKLARVSLNHGLYSFLRRNAPSLDEKVKEFSEAV 167

Query: 122 QEENGHSIS 130
            +E+  S+S
Sbjct: 168 GKEDDLSVS 176


>At5g45150.1 68418.m05543 ribonuclease III family protein similar to
           CAF protein (RNA helicase/RNAseIII) [Arabidopsis
           thaliana] GI:6102610; contains Pfam profiles PF00035:
           Double-stranded RNA binding motif, PF00636 RNase3 domain
          Length = 957

 Score = 59.7 bits (138), Expect = 9e-10
 Identities = 34/112 (30%), Positives = 58/112 (51%), Gaps = 7/112 (6%)

Query: 9   LHGYEQLERVLQYRFRDRSLLLQAMTHASHHRNVLTDCYQRLEFLGDAILDYLITRHLYE 68
           ++  E +E++L Y F +++LL +A+T  S         + RLEF GD+IL+   T ++  
Sbjct: 1   MNSVEAVEKILNYSFVNKTLLKEAITQKS-------PLFDRLEFFGDSILEVAFTNYICH 53

Query: 69  DKRCHSPGALTDLRSALVNNTIFATLAARHGFHKYFRHMSPGLNEVLKKYVK 120
                    L DLR+A V+N  FA +A  H  H +    +P L + +K + +
Sbjct: 54  TYPNLKVKELRDLRTANVSNEKFARIAVNHNLHHFLLLQNPSLFKKVKNFAE 105



 Score = 46.8 bits (106), Expect = 7e-06
 Identities = 28/109 (25%), Positives = 56/109 (51%), Gaps = 6/109 (5%)

Query: 13  EQLERVLQYRFRDRSLLLQAMTHASHHRNVLTDCYQRLEFLGDAILDYLITRHLYEDKRC 72
           E +E++L Y F +++LL + +TH +      +  +Q L F+G++ L    T+HLY     
Sbjct: 416 EAVEKILNYSFVNKTLLKELLTHNN------SPLFQGLMFVGESALSLAFTKHLYLTYPM 469

Query: 73  HSPGALTDLRSALVNNTIFATLAARHGFHKYFRHMSPGLNEVLKKYVKI 121
             P  L+ LR A   +  +A +A + G ++ F    P   ++   ++++
Sbjct: 470 LEPKDLSVLRDANTCHDKYACVAVKKGIYQSFIGSVPKPEKMTTDFIEL 518


>At5g53280.1 68418.m06622 expressed protein
          Length = 272

 Score = 32.7 bits (71), Expect = 0.12
 Identities = 23/96 (23%), Positives = 42/96 (43%), Gaps = 4/96 (4%)

Query: 9   LHGYEQLERVLQYRFRDRSLLLQAMTHASHHRNVLTDCYQRLEFLGD--AILDYLITRHL 66
           +H  ++ E+ +     ++S +L AM  A HH        + L F+G   +   Y+   HL
Sbjct: 104 IHTQQRTEKDVAIARLEQSRILLAMRLAEHHGKNYGVLEEALAFVGSIKSNSHYVSPDHL 163

Query: 67  YEDKRCHSPGALTDLRSALVNNTIFATLAARHGFHK 102
           Y+  R  +P     +   + +N +    A+  GF K
Sbjct: 164 YDSSR--NPDGANSIPDGIESNFVINAFASTFGFAK 197


>At4g01800.1 68417.m00237 preprotein translocase secA subunit,
           putative similar to preprotein translocase secA subunit,
           chloroplast [precursor] SP:Q9SYI0 from [Arabidopsis
           thaliana]; non-consensus GA donor splice site at exon 4
          Length = 1022

 Score = 27.9 bits (59), Expect = 3.3
 Identities = 14/40 (35%), Positives = 22/40 (55%), Gaps = 2/40 (5%)

Query: 9   LHGYEQLERVLQYRFRDRSLLLQAMTHASHHRNVLTDCYQ 48
           L GY Q + +++Y+    +L L+ M  A   RNV+   YQ
Sbjct: 940 LRGYAQRDPLIEYKLEGYNLFLEMM--AQIRRNVIYSIYQ 977


>At1g30270.1 68414.m03702 CBL-interacting protein kinase 23 (CIPK23)
           identical to CBL-interacting protein kinase 23
           [Arabidopsis thaliana] gi|14486386|gb|AAK61494
          Length = 482

 Score = 27.9 bits (59), Expect = 3.3
 Identities = 8/27 (29%), Positives = 20/27 (74%)

Query: 100 FHKYFRHMSPGLNEVLKKYVKIQEENG 126
           FHK++++++ GL +++ K +  ++E G
Sbjct: 436 FHKFYKNLTTGLKDIVWKTIDEEKEEG 462


  Database: arabidopsis
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.322    0.134    0.406 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,726,464
Number of Sequences: 28952
Number of extensions: 136983
Number of successful extensions: 396
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 377
Number of HSP's gapped (non-prelim): 14
length of query: 157
length of database: 12,070,560
effective HSP length: 75
effective length of query: 82
effective length of database: 9,899,160
effective search space: 811731120
effective search space used: 811731120
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.9 bits)
S2: 56 (26.6 bits)