Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA001833-TA|BGIBMGA001833-PA|IPR003100|Argonaute and Dicer
protein, PAZ, IPR000999|Ribonuclease III
(1126 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g43920.1 68416.m04701 ribonuclease III family protein similar... 95 3e-19
At1g01040.1 68414.m00004 DEAD/DEAH box helicase carpel factory /... 92 2e-18
At3g03300.1 68416.m00327 DEAD/DEAH box helicase carpel factory-r... 87 5e-17
At5g20320.1 68418.m02418 DEAD/DEAH box helicase, putative simila... 74 5e-13
At3g20420.1 68416.m02586 ribonuclease III family protein similar... 50 9e-06
At5g45150.1 68418.m05543 ribonuclease III family protein similar... 39 0.016
At2g29210.1 68415.m03550 splicing factor PWI domain-containing p... 35 0.27
At1g77280.1 68414.m09000 protein kinase family protein contains ... 32 2.5
At5g54560.1 68418.m06792 hypothetical protein contains Pfam prof... 31 3.3
At4g18905.1 68417.m02787 transducin family protein / WD-40 repea... 31 3.3
At4g35370.1 68417.m05025 transducin family protein / WD-40 repea... 31 5.7
>At3g43920.1 68416.m04701 ribonuclease III family protein similar to
RNA helicase/RNAseIII CAF protein [Arabidopsis thaliana]
GI:6102610; contains Pfam profiles PF02170: PAZ domain,
PF00636: RNase3 domain
Length = 1531
Score = 94.7 bits (225), Expect = 3e-19
Identities = 57/172 (33%), Positives = 90/172 (52%), Gaps = 2/172 (1%)
Query: 865 SPSVILQALTMSNANDGINLERLETVGDSFLKFAITAYLYCAHPNVHEGKLSHMRSKQVS 924
S + IL+A+T + ++ERLE +GDS LK+ + +L+ +P+ EG+LS R +S
Sbjct: 953 SSTSILEAVTTLTCPESFSMERLELLGDSVLKYVASCHLFLKYPDKDEGQLSRQRQSIIS 1012
Query: 925 NLNLYRLGRNKQLGSRMIASKFEPHDNWLPPCHEPPPTLQPKLNLNGQDKKQNVDSSPEN 984
N NL+RL +++L + FEP W P + K ++ ++ + EN
Sbjct: 1013 NSNLHRLTTSRKLQGYIRNGAFEPR-RWTAPGQFSLFPVPCKCGIDTREVPLDPKFFTEN 1071
Query: 985 VGCFIPYNLITQHS-IPDKSIADCVEALIGAYLLECGPRGALLFMSWLGIAV 1035
+ I + H + KS++DC EALIGAY + G +L M WLGI V
Sbjct: 1072 MTIKIGKSCDMGHRWVVSKSVSDCAEALIGAYYVSGGLSASLHMMKWLGIDV 1123
Score = 48.0 bits (109), Expect = 4e-05
Identities = 28/121 (23%), Positives = 57/121 (47%), Gaps = 4/121 (3%)
Query: 331 LSPDSVFPSASHATFRDYYQNKYGVTITQSKQPLLDVDHTSARLNLLTPRYVNRKGVALP 390
+SP V S+ +AT+ +Y+ KYG+ + QPL+ + + NLL N + V
Sbjct: 827 MSPFEVDASSGYATYAEYFNKKYGIVLAHPNQPLMKLKQSHHAHNLLVD--FNEEMVV-- 882
Query: 391 VSSERTRRAKRERLDQKQLLLPELCRAHPFAAPLWAATVALPCALYRINALLIAEEIRRS 450
+ + ++ + + L PEL + + LP ++R+ +L++A ++R
Sbjct: 883 KTEPKAGNVRKRKPNIHAHLPPELLARIDVPRAVLKSIYLLPSVMHRLESLMLASQLREE 942
Query: 451 V 451
+
Sbjct: 943 I 943
Score = 45.6 bits (103), Expect = 2e-04
Identities = 23/81 (28%), Positives = 44/81 (54%)
Query: 861 HPGPSPSVILQALTMSNANDGINLERLETVGDSFLKFAITAYLYCAHPNVHEGKLSHMRS 920
H + ++ +A+T S+ + + ERLE +GDS L F IT +L+ + G+++ +RS
Sbjct: 1158 HEFSAKFLLKEAITHSSLRESYSYERLEFLGDSVLDFLITRHLFNTYEQTGPGEMTDLRS 1217
Query: 921 KQVSNLNLYRLGRNKQLGSRM 941
V+N N ++ L + +
Sbjct: 1218 ACVNNENFAQVAVKNNLHTHL 1238
>At1g01040.1 68414.m00004 DEAD/DEAH box helicase carpel factory / CAF
identical to RNA helicase/RNAseIII CAF protein
GB:AAF03534 GI:6102610 from [Arabidopsis thaliana]
Length = 1909
Score = 91.9 bits (218), Expect = 2e-18
Identities = 63/189 (33%), Positives = 95/189 (50%), Gaps = 15/189 (7%)
Query: 858 LEGHPGPSPSVILQALTMSNANDGINLERLETVGDSFLKFAITAYLYCAHPNVHEGKLSH 917
L +P P+ S IL+ALT ++ + ER E +GD++LK+ ++ +L+ +P HEG+L+
Sbjct: 1349 LISYPIPT-SKILEALTAASCQETFCYERAELLGDAYLKWVVSRFLFLKYPQKHEGQLTR 1407
Query: 918 MRSKQVSNLNLYRLGRNKQLGSRMIASKFEPHDNWLPPCHEPPPTLQPKLNLNGQD--KK 975
MR + VSN+ LY+ K L S + A +F P W P PP G +
Sbjct: 1408 MRQQMVSNMVLYQFALVKGLQSYIQADRFAP-SRWSAP--GVPPVFDEDTKDGGSSFFDE 1464
Query: 976 QNVDSSPENVGCF---------IPYNLITQHSIPDKSIADCVEALIGAYLLECGPRGALL 1026
+ S EN F + +L + + K++AD VEALIG Y +E G A
Sbjct: 1465 EQKPVSEENSDVFEDGEMEDGELEGDLSSYRVLSSKTLADVVEALIGVYYVEGGKIAANH 1524
Query: 1027 FMSWLGIAV 1035
M W+GI V
Sbjct: 1525 LMKWIGIHV 1533
Score = 57.2 bits (132), Expect = 6e-08
Identities = 40/141 (28%), Positives = 68/141 (48%), Gaps = 18/141 (12%)
Query: 322 FLVAEICWRLSPDSVFPSAS-------HATFRDYYQNKYGVTITQSKQPLLDVDHTSARL 374
F V IC+ +S ++ FP + T+ DYY+ KYGV + +QPL+ S
Sbjct: 1225 FYVDSICYDMSAETSFPRKEGYLGPLEYNTYADYYKQKYGVDLNCKQQPLIKGRGVSYCK 1284
Query: 375 NLLTPRYVNRKGVALPVSSERTRRAKRERLDQKQLLLPELCRAHPFAAPLWAATVALPCA 434
NLL+PR+ + G + V ++T L PELC HP + L LP
Sbjct: 1285 NLLSPRF-EQSGESETV-LDKTYYV---------FLPPELCVVHPLSGSLIRGAQRLPSI 1333
Query: 435 LYRINALLIAEEIRRSVAVEV 455
+ R+ ++L+A +++ ++ +
Sbjct: 1334 MRRVESMLLAVQLKNLISYPI 1354
Score = 40.3 bits (90), Expect = 0.007
Identities = 22/72 (30%), Positives = 44/72 (61%), Gaps = 2/72 (2%)
Query: 868 VILQALT-MSNANDGINL-ERLETVGDSFLKFAITAYLYCAHPNVHEGKLSHMRSKQVSN 925
++++A+T S + G++ +RLE VGD+ L IT +L+ + ++ G+L+ +R+ V+N
Sbjct: 1575 LLVEAITHASRPSSGVSCYQRLEFVGDAVLDHLITRHLFFTYTSLPPGRLTDLRAAAVNN 1634
Query: 926 LNLYRLGRNKQL 937
N R+ +L
Sbjct: 1635 ENFARVAVKHKL 1646
>At3g03300.1 68416.m00327 DEAD/DEAH box helicase carpel
factory-related similar to RNA helicase GB:AAF03534
Length = 1317
Score = 87.4 bits (207), Expect = 5e-17
Identities = 58/167 (34%), Positives = 88/167 (52%), Gaps = 17/167 (10%)
Query: 869 ILQALTMSNANDGINLERLETVGDSFLKFAITAYLYCAHPNVHEGKLSHMRSKQVSNLNL 928
+L+A+T D +LE LET+GDSFLK+A+ L+ HEG LS + +SN+ L
Sbjct: 908 VLEAITTKKCEDQFHLESLETLGDSFLKYAVCQQLFQHCHTHHEGLLSTKKDGMISNVML 967
Query: 929 YRLGRNKQLGSRMIASKFEPHDNWLPPCHEPPPTLQPKLNLNGQDKKQNVDSSPENVGCF 988
+ G ++L + FEP W+ P +N D+ PE+ +
Sbjct: 968 CQFGCQQKLQGFIRDECFEP-KGWMVPGQS--SAAYSLVN----------DTLPESRNIY 1014
Query: 989 IPYNLITQHSIPDKSIADCVEALIGAYLLECGPRGALLFMSWLGIAV 1035
+ ++ ++ KS+AD VE+LIGAYL E G AL+FM+W+GI V
Sbjct: 1015 V----ASRRNLKRKSVADVVESLIGAYLSEGGELAALMFMNWVGIKV 1057
Score = 39.9 bits (89), Expect = 0.009
Identities = 19/41 (46%), Positives = 27/41 (65%)
Query: 885 ERLETVGDSFLKFAITAYLYCAHPNVHEGKLSHMRSKQVSN 925
+RLE +GDS L + IT +LY +P + G L+ MRS V+N
Sbjct: 1114 QRLEFLGDSVLDYLITKHLYDKYPCLSPGLLTDMRSASVNN 1154
>At5g20320.1 68418.m02418 DEAD/DEAH box helicase, putative similar to
CAF protein [Arabidopsis thaliana] GI:6102610; contains
Pfam profiles PF00270: DEAD/DEAH box helicase, PF00271:
Helicase conserved C-terminal domain, PF03368: Domain of
unknown function, PF00636: RNase3 domain, PF00035:
Double-stranded RNA binding motif
Length = 1676
Score = 74.1 bits (174), Expect = 5e-13
Identities = 53/167 (31%), Positives = 90/167 (53%), Gaps = 20/167 (11%)
Query: 869 ILQALTMSNANDGINLERLETVGDSFLKFAITAYLYCAHPNVHEGKLSHMRSKQVSNLNL 928
+L+ALT ++ ++LERLE +GD+FLKFA++ +L+ H ++ EG+L+ RS N+
Sbjct: 1094 VLEALTTEKCHERLSLERLEVLGDAFLKFAVSRHLFLHHDSLDEGELTRRRS------NV 1147
Query: 929 YRLGRNKQLGSRMIASKFEPHDNWLPPCHEPPPTLQPKLNLNGQDKKQNVDSSPENVGCF 988
Y R++ L + P C E LN + ++S+ + C
Sbjct: 1148 YI--RDQALDPTQFFAFGHP---CRVTCDEVASKEVHSLNRD----LGILESNTGEIRCS 1198
Query: 989 IPYNLITQHSIPDKSIADCVEALIGAYLLECGPRGALLFMSWLGIAV 1035
+ H + K+IAD VEAL+GA+L++ G +GA+ F+ W+G+ V
Sbjct: 1199 KGH-----HWLYKKTIADVVEALVGAFLVDSGFKGAVKFLKWIGVNV 1240
Score = 39.1 bits (87), Expect = 0.016
Identities = 17/49 (34%), Positives = 30/49 (61%)
Query: 877 NANDGINLERLETVGDSFLKFAITAYLYCAHPNVHEGKLSHMRSKQVSN 925
N + G +RLE +GD+ L + +T+Y + P + G+L+ +RS V+N
Sbjct: 1293 NRHGGGCYQRLEFLGDAVLDYLMTSYFFTVFPKLKPGQLTDLRSLSVNN 1341
>At3g20420.1 68416.m02586 ribonuclease III family protein similar to
CAF protein (RNA helicase/RNAseIII) [Arabidopsis
thaliana] GI:6102610; contains Pfam profiles: PF00636
RNase3 domain, PF00035 Double-stranded RNA binding motif
Length = 391
Score = 50.0 bits (114), Expect = 9e-06
Identities = 27/73 (36%), Positives = 43/73 (58%)
Query: 867 SVILQALTMSNANDGINLERLETVGDSFLKFAITAYLYCAHPNVHEGKLSHMRSKQVSNL 926
S++ +A+T ++ D + ERLE +GDS + AI+ YLY +P++ LS +R+ VS
Sbjct: 75 SLLKEAITHTSCTDFPSYERLEFIGDSAIGLAISNYLYLTYPSLEPHDLSLLRAANVSTE 134
Query: 927 NLYRLGRNKQLGS 939
L R+ N L S
Sbjct: 135 KLARVSLNHGLYS 147
>At5g45150.1 68418.m05543 ribonuclease III family protein similar to
CAF protein (RNA helicase/RNAseIII) [Arabidopsis
thaliana] GI:6102610; contains Pfam profiles PF00035:
Double-stranded RNA binding motif, PF00636 RNase3 domain
Length = 957
Score = 39.1 bits (87), Expect = 0.016
Identities = 20/53 (37%), Positives = 29/53 (54%)
Query: 885 ERLETVGDSFLKFAITAYLYCAHPNVHEGKLSHMRSKQVSNLNLYRLGRNKQL 937
+RLE GDS L+ A T Y+ +PN+ +L +R+ VSN R+ N L
Sbjct: 33 DRLEFFGDSILEVAFTNYICHTYPNLKVKELRDLRTANVSNEKFARIAVNHNL 85
>At2g29210.1 68415.m03550 splicing factor PWI domain-containing
protein contains Pfam profile PF01480: PWI domain
Length = 878
Score = 35.1 bits (77), Expect = 0.27
Identities = 28/89 (31%), Positives = 33/89 (37%), Gaps = 6/89 (6%)
Query: 1038 RHLAALP---HTHSTSEQRETSAEPPPARRRVGSLPPYRDRQGNWVQQVYGELKAPPSPL 1094
RH + P S S PPPARRR PP R R+ + PSPL
Sbjct: 339 RHRSPTPPARQRRSPSPPARRHRSPPPARRRRSPSPPARRRRS---PSPPARRRRSPSPL 395
Query: 1095 LRYVEDPEGELEKMLSGKELEQSAYSDSP 1123
R P + S L + SDSP
Sbjct: 396 YRRNRSPSPLYRRNRSRSPLAKRGRSDSP 424
>At1g77280.1 68414.m09000 protein kinase family protein contains Pfam
domain, PF00069: Protein kinase domain
Length = 794
Score = 31.9 bits (69), Expect = 2.5
Identities = 24/61 (39%), Positives = 30/61 (49%), Gaps = 6/61 (9%)
Query: 961 PTLQPKLNLNGQDKKQ-NVDSSPENVG---CFIPY--NLITQHSIPDKSIADCVEALIGA 1014
P +Q L L +D KQ DSS +N+ +P+ N IT SIPD S E L G
Sbjct: 363 PVVQWALKLPPRDTKQLGYDSSEDNLSTLNALVPFGINSITNKSIPDNSPRKLPEELEGL 422
Query: 1015 Y 1015
Y
Sbjct: 423 Y 423
>At5g54560.1 68418.m06792 hypothetical protein contains Pfam profile
PF03478: Protein of unknown function (DUF295)
Length = 360
Score = 31.5 bits (68), Expect = 3.3
Identities = 18/55 (32%), Positives = 27/55 (49%)
Query: 552 MASSIPEDTDFDEYLEPLPPNLTFCTSASGSASWCDPIQKPKSEFNPTRSYSVAD 606
M+SS PED + ++ L P L+FC A ++ W + S F+ YS D
Sbjct: 142 MSSSCPEDENCVVAVKFLGPQLSFCRPAQSNSEWTNIKIASPSFFSSDVMYSKKD 196
>At4g18905.1 68417.m02787 transducin family protein / WD-40 repeat
family protein contains 5 (4 significant) WD-40 repeats;
similar to periodic tryptophan protein 1 homolog
(Keratinocyte protein IEF SSP 9502) (PWP1)(SP:Q13610)
(PIR2:I39360) [Homo sapiens]
Length = 494
Score = 31.5 bits (68), Expect = 3.3
Identities = 14/39 (35%), Positives = 21/39 (53%)
Query: 803 MKDIDIDAPDYVDEKITNIGFDDGDKYNTGNEFKPYYED 841
+K++D+D D D++I GD Y NE PY +D
Sbjct: 94 LKELDMDNYDEEDDEIELFSSGMGDLYYPSNEMDPYLKD 132
>At4g35370.1 68417.m05025 transducin family protein / WD-40 repeat
family protein contains 4 (3 significant) WD-40 repeats;
similar to periodic tryptophan protein 1 homolog
(Keratinocyte protein IEF SSP 9502) (PWP1)(SP:Q13610)
(PIR2:I39360) [Homo sapiens]
Length = 414
Score = 30.7 bits (66), Expect = 5.7
Identities = 14/47 (29%), Positives = 22/47 (46%)
Query: 803 MKDIDIDAPDYVDEKITNIGFDDGDKYNTGNEFKPYYEDDKGGQKRE 849
+K++D+D D D++I G Y N+ PY +D G E
Sbjct: 69 LKELDMDNYDEEDDEIELFSSGQGHLYYPSNDMDPYLKDTDGDYDSE 115
Database: arabidopsis
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.316 0.134 0.407
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 24,700,367
Number of Sequences: 28952
Number of extensions: 1017984
Number of successful extensions: 2442
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 2404
Number of HSP's gapped (non-prelim): 33
length of query: 1126
length of database: 12,070,560
effective HSP length: 89
effective length of query: 1037
effective length of database: 9,493,832
effective search space: 9845103784
effective search space used: 9845103784
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)
S2: 65 (30.3 bits)
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