Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA001725-TA|BGIBMGA001725-PA|IPR001030|Aconitate
hydratase, N-terminal
(419 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g35830.1 68417.m05090 aconitate hydratase, cytoplasmic / citr... 485 e-137
At4g26970.1 68417.m03881 aconitate hydratase, cytoplasmic, putat... 485 e-137
At2g05710.1 68415.m00611 aconitate hydratase, cytoplasmic, putat... 476 e-134
At4g13430.1 68417.m02096 aconitase family protein / aconitate hy... 59 5e-09
>At4g35830.1 68417.m05090 aconitate hydratase, cytoplasmic / citrate
hydro-lyase / aconitase (ACO) identical to SP|Q42560
Aconitate hydratase, cytoplasmic (EC 4.2.1.3) (Citrate
hydro-lyase) (Aconitase) {Arabidopsis thaliana};
contains Pfam profiles PF00330: Aconitase family
(aconitate hydratase), PF00694: Aconitase C-terminal
domain
Length = 898
Score = 485 bits (1197), Expect = e-137
Identities = 241/400 (60%), Positives = 294/400 (73%), Gaps = 6/400 (1%)
Query: 4 STNPYQKLLKTIEI--NGSTYTYYDVSALG-PKYDRLPFSIRVLLESCVRNCDNFQVLEK 60
S NP++ +LK +E G YY + AL P+ D+LP+SIR+LLES +RNCD FQV K
Sbjct: 3 SENPFRSILKALEKPDGGEFGNYYSLPALNDPRIDKLPYSIRILLESAIRNCDEFQVKSK 62
Query: 61 DVSNVLDWESKQGNEESVEIAFKPARVILQDLTGVPAVVDFAAMRDAVKDLGGDPDRINP 120
DV +LDWE+ + + VEI FKPARV+LQD TGVPAVVD A MRDA+ +LGGD ++INP
Sbjct: 63 DVEKILDWENT--SPKQVEIPFKPARVLLQDFTGVPAVVDLACMRDAMNNLGGDSNKINP 120
Query: 121 ICPADLVIDHSVQVDFARTPDALNKNQDLEFVRNKERFQFLKWGAQAFDNMLIVPPGSGI 180
+ P DLVIDHSVQVD AR+ +A+ N +LEF RNKERF FLKWG+ AF NML+VPPGSGI
Sbjct: 121 LVPVDLVIDHSVQVDVARSENAVQANMELEFQRNKERFAFLKWGSNAFHNMLVVPPGSGI 180
Query: 181 VHQVNLEYLARVVF-TGPLLHPDSVVGTDSHTTMINXXXXXXXXXXXIEAEAVMLGQAIS 239
VHQVNLEYLARVVF T LL+PDSVVGTDSHTTMI+ IEAEA MLGQ +S
Sbjct: 181 VHQVNLEYLARVVFNTNGLLYPDSVVGTDSHTTMIDGLGVAGWGVGGIEAEATMLGQPMS 240
Query: 240 MLLPKVVGYKLVGELNPLATSTDLVLTITKHLRSLGVVGKFVEFFGPGVGALSIADRATV 299
M+LP VVG+KL G+L T+TDLVLT+T+ LR GVVGKFVEF G G+ LS+ADRAT+
Sbjct: 241 MVLPGVVGFKLTGKLRDGMTATDLVLTVTQMLRKHGVVGKFVEFHGEGMRELSLADRATI 300
Query: 300 ANMCPEFGATLAHFPVDARSLEYLHQTNRPKEKIDVIESYLRATKQFRDYTNPEQDPVFS 359
ANM PE+GAT+ FPVD +L+YL T R + + +IE+YLRA K F DY+ PE V+S
Sbjct: 301 ANMSPEYGATMGFFPVDHVTLQYLRLTGRSDDTVSMIEAYLRANKMFVDYSEPESKTVYS 360
Query: 360 EVVELDLGDVVTSVSGPKRPQDRVSVAIMKKDFQDCLTNK 399
+EL+L DV VSGPKRP DRV + MK D+ CL N+
Sbjct: 361 SCLELNLEDVEPCVSGPKRPHDRVPLKEMKADWHSCLDNR 400
>At4g26970.1 68417.m03881 aconitate hydratase, cytoplasmic, putative
/ citrate hydro-lyase/aconitase, putative strong
similarity to SP|P49608 Aconitate hydratase, cytoplasmic
(EC 4.2.1.3) (Citrate hydro-lyase) (Aconitase)
{Cucurbita maxima}; contains Pfam profiles PF00330:
Aconitase family (aconitate hydratase), PF00694:
Aconitase C-terminal domain
Length = 995
Score = 485 bits (1195), Expect = e-137
Identities = 244/422 (57%), Positives = 303/422 (71%), Gaps = 10/422 (2%)
Query: 4 STNPYQKLLKTI-EINGSTY-TYYDVSALG-PKYDRLPFSIRVLLESCVRNCDNFQVLEK 60
S + Y+ +L ++ + G Y YY + AL P+ D+LPFS+R+LLES +RNCDN+QV +
Sbjct: 100 SEHSYKDILTSLPKPGGGEYGKYYSLPALNDPRIDKLPFSVRILLESAIRNCDNYQVTKD 159
Query: 61 DVSNVLDWESKQGNEESVEIAFKPARVILQDLTGVPAVVDFAAMRDAVKDLGGDPDRINP 120
DV +LDWE+ + + VEIAFKPARVILQD TGVP +VD A+MRDAVK+LG DP +INP
Sbjct: 160 DVEKILDWENT--STKQVEIAFKPARVILQDFTGVPVLVDLASMRDAVKNLGSDPSKINP 217
Query: 121 ICPADLVIDHSVQVDFARTPDALNKNQDLEFVRNKERFQFLKWGAQAFDNMLIVPPGSGI 180
+ P DLV+DHS+QVDFAR+ DA KN +LEF RNKERF FLKWG+ AF NML+VPPGSGI
Sbjct: 218 LVPVDLVVDHSIQVDFARSEDAAQKNLELEFKRNKERFTFLKWGSTAFQNMLVVPPGSGI 277
Query: 181 VHQVNLEYLARVVFTGP-LLHPDSVVGTDSHTTMINXXXXXXXXXXXIEAEAVMLGQAIS 239
VHQVNLEYL RVVF L+PDSVVGTDSHTTMI+ IEAEA MLGQ +S
Sbjct: 278 VHQVNLEYLGRVVFNSKGFLYPDSVVGTDSHTTMIDGLGVAGWGVGGIEAEAAMLGQPMS 337
Query: 240 MLLPKVVGYKLVGELNPLATSTDLVLTITKHLRSLGVVGKFVEFFGPGVGALSIADRATV 299
M+LP VVG+KL G+L T+TDLVLT+T+ LR GVVGKFVEF+G G+ LS+ADRAT+
Sbjct: 338 MVLPGVVGFKLDGKLKEGVTATDLVLTVTQILRKHGVVGKFVEFYGEGMSELSLADRATI 397
Query: 300 ANMCPEFGATLAHFPVDARSLEYLHQTNRPKEKIDVIESYLRATKQFRDYTNPEQDPVFS 359
ANM PE+GAT+ FPVD +LEYL T R E + +IESYLRA F DY P+Q+ ++
Sbjct: 398 ANMSPEYGATMGFFPVDHVTLEYLKLTGRSDETVSMIESYLRANNMFVDYNEPQQERAYT 457
Query: 360 EVVELDLGDVVTSVSGPKRPQDRVSVAIMKKDFQDCLTNKPV---PIAILSGVQEHVLDF 416
++L+LG V +SGPKRP DRV + MK D+ CL N PV A+ QE V+ F
Sbjct: 458 SYLQLELGHVEPCISGPKRPHDRVPLKDMKADWHACLDN-PVGFKGFAVPKEKQEEVVKF 516
Query: 417 PY 418
Y
Sbjct: 517 SY 518
>At2g05710.1 68415.m00611 aconitate hydratase, cytoplasmic, putative
/ citrate hydro-lyase/aconitase, putative nearly
identical to SP|P49608 Aconitate hydratase, cytoplasmic
(EC 4.2.1.3) (Citrate hydro-lyase) (Aconitase)
{Cucurbita maxima}; contains Pfam profiles PF00330:
Aconitase family (aconitate hydratase), PF00694:
Aconitase C-terminal domain
Length = 990
Score = 476 bits (1174), Expect = e-134
Identities = 239/421 (56%), Positives = 301/421 (71%), Gaps = 8/421 (1%)
Query: 4 STNPYQKLLKTIEI--NGSTYTYYDVSALG-PKYDRLPFSIRVLLESCVRNCDNFQVLEK 60
S +P++ + T+ G +Y + AL P+ D+LP+SIR+LLES +RNCDNFQV ++
Sbjct: 95 SEHPFKGIFTTLPKPGGGEFGKFYSLPALNDPRVDKLPYSIRILLESAIRNCDNFQVTKE 154
Query: 61 DVSNVLDWESKQGNEESVEIAFKPARVILQDLTGVPAVVDFAAMRDAVKDLGGDPDRINP 120
DV ++DWE + + VEI FKPARV+LQD TGVPAVVD A MRDA+ LG D ++INP
Sbjct: 155 DVEKIIDWEKT--SPKQVEIPFKPARVLLQDFTGVPAVVDLACMRDAMNKLGSDSNKINP 212
Query: 121 ICPADLVIDHSVQVDFARTPDALNKNQDLEFVRNKERFQFLKWGAQAFDNMLIVPPGSGI 180
+ P DLVIDHSVQVD AR+ +A+ N +LEF RNKERF FLKWG+ AF NML+VPPGSGI
Sbjct: 213 LVPVDLVIDHSVQVDVARSENAVQANMELEFQRNKERFAFLKWGSTAFQNMLVVPPGSGI 272
Query: 181 VHQVNLEYLARVVF-TGPLLHPDSVVGTDSHTTMINXXXXXXXXXXXIEAEAVMLGQAIS 239
VHQVNLEYL RVVF T LL+PDSVVGTDSHTTMI+ IEAEA MLGQ +S
Sbjct: 273 VHQVNLEYLGRVVFNTKGLLYPDSVVGTDSHTTMIDGLGVAGWGVGGIEAEATMLGQPMS 332
Query: 240 MLLPKVVGYKLVGELNPLATSTDLVLTITKHLRSLGVVGKFVEFFGPGVGALSIADRATV 299
M+LP VVG+KL G++ T+TDLVLT+T+ LR GVVGKFVEF+G G+ LS+ADRAT+
Sbjct: 333 MVLPGVVGFKLAGKMRNGVTATDLVLTVTQMLRKHGVVGKFVEFYGNGMSGLSLADRATI 392
Query: 300 ANMCPEFGATLAHFPVDARSLEYLHQTNRPKEKIDVIESYLRATKQFRDYTNPEQDPVFS 359
ANM PE+GAT+ FPVD +L+YL T R E + +IE+YLRA F DY P+QD V+S
Sbjct: 393 ANMSPEYGATMGFFPVDHVTLQYLKLTGRSDETVAMIEAYLRANNMFVDYNEPQQDRVYS 452
Query: 360 EVVELDLGDVVTSVSGPKRPQDRVSVAIMKKDFQDCLTNKP--VPIAILSGVQEHVLDFP 417
+EL+L DV +SGPKRP DRV++ MK D+ CL +K AI QE V++F
Sbjct: 453 SYLELNLDDVEPCISGPKRPHDRVTLKEMKADWHSCLDSKVGFKGFAIPKEAQEKVVNFS 512
Query: 418 Y 418
+
Sbjct: 513 F 513
>At4g13430.1 68417.m02096 aconitase family protein / aconitate
hydratase family protein contains Pfam profile PF00330:
Aconitase family (aconitate hydratase
Length = 509
Score = 59.3 bits (137), Expect = 5e-09
Identities = 38/131 (29%), Positives = 62/131 (47%), Gaps = 1/131 (0%)
Query: 204 VVGTDSHTTMINXXXXXXXXXXXIEAEAVMLGQAISMLLPKVVGYKLVGELNPLATSTDL 263
++GTDSHT +A V+ I + +P + + L GE+ + DL
Sbjct: 195 LLGTDSHTCTAGAFGQFATGIGNTDAGFVLGTGKILLKVPPTMRFILDGEMPSYLQAKDL 254
Query: 264 VLTITKHLRSLGVVGKFVEFFGPGVGALSIADRATVANMCPEFGATLAHFPVDARSLEYL 323
+L I + G K +EF G + +LS+ +R T+ NM E G P DA +L Y+
Sbjct: 255 ILQIIGEISVAGATYKTMEFSGTTIESLSMEERMTLCNMVVEAGGKNGVIPPDATTLNYV 314
Query: 324 -HQTNRPKEKI 333
++T+ P E +
Sbjct: 315 ENRTSVPFEPV 325
Database: arabidopsis
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.319 0.137 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,620,167
Number of Sequences: 28952
Number of extensions: 393534
Number of successful extensions: 959
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 949
Number of HSP's gapped (non-prelim): 4
length of query: 419
length of database: 12,070,560
effective HSP length: 83
effective length of query: 336
effective length of database: 9,667,544
effective search space: 3248294784
effective search space used: 3248294784
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.8 bits)
S2: 61 (28.7 bits)
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