BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA001401-TA|BGIBMGA001401-PA|IPR003000|Silent information regulator protein Sir2 (370 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g55760.1 68418.m06950 transcriptional regulator Sir2 family p... 256 2e-68 At5g09230.5 68418.m01052 transcriptional regulator Sir2 family p... 50 2e-06 At5g09230.3 68418.m01049 transcriptional regulator Sir2 family p... 50 2e-06 At5g09230.2 68418.m01051 transcriptional regulator Sir2 family p... 50 2e-06 At5g09230.1 68418.m01050 transcriptional regulator Sir2 family p... 50 2e-06 At5g09230.6 68418.m01054 transcriptional regulator Sir2 family p... 42 7e-04 At5g09230.4 68418.m01053 transcriptional regulator Sir2 family p... 42 7e-04 At5g48690.1 68418.m06025 hypothetical protein 34 0.17 At3g55340.1 68416.m06146 RNA recognition motif (RRM)-containing ... 33 0.40 At3g48970.1 68416.m05349 copper-binding family protein similar t... 30 2.8 At4g14020.1 68417.m02167 rapid alkalinization factor (RALF) fami... 29 3.8 At3g55960.1 68416.m06218 NLI interacting factor (NIF) family pro... 29 3.8 At2g46070.1 68415.m05731 mitogen-activated protein kinase, putat... 29 3.8 At3g28770.1 68416.m03591 expressed protein 29 5.0 At5g07270.1 68418.m00829 ankyrin repeat family protein contains ... 29 6.6 At1g42580.1 68414.m04910 hypothetical protein contains similarit... 29 6.6 At5g05440.1 68418.m00586 expressed protein low similarity to cyt... 28 8.7 >At5g55760.1 68418.m06950 transcriptional regulator Sir2 family protein contains Pfam domain PF02146: transcriptional regulator, Sir2 family Length = 473 Score = 256 bits (626), Expect = 2e-68 Identities = 131/276 (47%), Positives = 178/276 (64%), Gaps = 6/276 (2%) Query: 1 MSCNYAEGLSPYENKGILGVPEKFDSNDKLNQKCVLLAQLVKDSKHIVVHTGAGISTSAG 60 MS YAE LS E+ G +G+ E FD + L K LA+L++ SKH+VV TGAGISTS G Sbjct: 1 MSLGYAEKLSFIEDVGQVGMAEFFDPSHLLQCKIEELAKLIQKSKHLVVFTGAGISTSCG 60 Query: 61 IPDFRGPNGVWTLEKEGKK-PTINVSFADAQPTKTHMXXXXXXXXXXXXXXVSQNIDGLH 119 IPDFRGP G+WTL++EGK P ++ F A P+ THM +SQN+DGLH Sbjct: 61 IPDFRGPKGIWTLQREGKDLPKASLPFHRAMPSMTHMALVELERAGILKFVISQNVDGLH 120 Query: 120 LKSGVPRKFLAELHGNMFIDECNICKRQFVRSSPVETVGKKCSGVPCAAHHVTGRPCRGR 179 L+SG+PR+ L+ELHG+ F++ C C +++R VET+G K + C+ C + Sbjct: 121 LRSGIPREKLSELHGDSFMEMCPSCGAEYLRDFEVETIGLKETSRKCSVEK-----CGAK 175 Query: 180 LYDGVLDWEHSLPENDLLMAEWHSSIADLSICLGTTLQIVPSGNLPLETIKYGGKLVICN 239 L D VLDWE +LP ++ AE H ADL +CLGT+LQI P+ NLPL+ +K GGK+VI N Sbjct: 176 LKDTVLDWEDALPPKEIDPAEKHCKKADLVLCLGTSLQITPACNLPLKCLKGGGKIVIVN 235 Query: 240 LQPTKHDNKADLLINYYVDDVLEKVMDILGIEIPSY 275 LQ T D KA+++I+ VD V+ VM+ L ++IP Y Sbjct: 236 LQKTPKDKKANVVIHGLVDKVVAGVMESLNMKIPPY 271 >At5g09230.5 68418.m01052 transcriptional regulator Sir2 family protein contains Pfam domain PF02146: transcriptional regulator, Sir2 family; Length = 354 Score = 50.4 bits (115), Expect = 2e-06 Identities = 39/135 (28%), Positives = 55/135 (40%), Gaps = 27/135 (20%) Query: 37 LAQLVKDSKHIVVHTGAGISTSAGIPDFRGPNGVWTLEKEGKKPTINVS----------- 85 L +L + S + + TGAG+ST GIPD+R PNG ++ G KP + Sbjct: 66 LYRLFEQSSRLTILTGAGVSTECGIPDYRSPNGAYS---SGFKPITHQEFTRSSRARRRY 122 Query: 86 ----------FADAQPTKTHMXXXXXXXXXXXXXXVSQNIDGLHLKSGVPRKFLAELHGN 135 F AQP H ++QN+D LH ++G ELHG Sbjct: 123 WARSYAGWRRFTAAQPGPAHTALASLEKAGRINFMITQNVDRLHHRAGSDP---LELHGT 179 Query: 136 MFIDECNICKRQFVR 150 ++ C C F R Sbjct: 180 VYTVMCLECGFSFPR 194 Score = 41.9 bits (94), Expect = 7e-04 Identities = 24/99 (24%), Positives = 49/99 (49%) Query: 176 CRGRLYDGVLDWEHSLPENDLLMAEWHSSIADLSICLGTTLQIVPSGNLPLETIKYGGKL 235 C+G L V+ + ++P+ A + +D + LG++L + + L + G Sbjct: 255 CKGVLKPDVIFFGDNIPKERATQAMEVAKQSDAFLVLGSSLMTMSAFRLCRAAHEAGAMT 314 Query: 236 VICNLQPTKHDNKADLLINYYVDDVLEKVMDILGIEIPS 274 I N+ T+ D+ L IN V ++L +V+D+ + +P+ Sbjct: 315 AIVNIGETRADDIVPLKINARVGEILHRVLDVGSLSVPA 353 >At5g09230.3 68418.m01049 transcriptional regulator Sir2 family protein contains Pfam domain PF02146: transcriptional regulator, Sir2 family; Length = 324 Score = 50.4 bits (115), Expect = 2e-06 Identities = 39/135 (28%), Positives = 55/135 (40%), Gaps = 27/135 (20%) Query: 37 LAQLVKDSKHIVVHTGAGISTSAGIPDFRGPNGVWTLEKEGKKPTINVS----------- 85 L +L + S + + TGAG+ST GIPD+R PNG ++ G KP + Sbjct: 85 LYRLFEQSSRLTILTGAGVSTECGIPDYRSPNGAYS---SGFKPITHQEFTRSSRARRRY 141 Query: 86 ----------FADAQPTKTHMXXXXXXXXXXXXXXVSQNIDGLHLKSGVPRKFLAELHGN 135 F AQP H ++QN+D LH ++G ELHG Sbjct: 142 WARSYAGWRRFTAAQPGPAHTALASLEKAGRINFMITQNVDRLHHRAGSDP---LELHGT 198 Query: 136 MFIDECNICKRQFVR 150 ++ C C F R Sbjct: 199 VYTVMCLECGFSFPR 213 >At5g09230.2 68418.m01051 transcriptional regulator Sir2 family protein contains Pfam domain PF02146: transcriptional regulator, Sir2 family; Length = 373 Score = 50.4 bits (115), Expect = 2e-06 Identities = 39/135 (28%), Positives = 55/135 (40%), Gaps = 27/135 (20%) Query: 37 LAQLVKDSKHIVVHTGAGISTSAGIPDFRGPNGVWTLEKEGKKPTINVS----------- 85 L +L + S + + TGAG+ST GIPD+R PNG ++ G KP + Sbjct: 85 LYRLFEQSSRLTILTGAGVSTECGIPDYRSPNGAYS---SGFKPITHQEFTRSSRARRRY 141 Query: 86 ----------FADAQPTKTHMXXXXXXXXXXXXXXVSQNIDGLHLKSGVPRKFLAELHGN 135 F AQP H ++QN+D LH ++G ELHG Sbjct: 142 WARSYAGWRRFTAAQPGPAHTALASLEKAGRINFMITQNVDRLHHRAGSDP---LELHGT 198 Query: 136 MFIDECNICKRQFVR 150 ++ C C F R Sbjct: 199 VYTVMCLECGFSFPR 213 Score = 41.9 bits (94), Expect = 7e-04 Identities = 24/99 (24%), Positives = 49/99 (49%) Query: 176 CRGRLYDGVLDWEHSLPENDLLMAEWHSSIADLSICLGTTLQIVPSGNLPLETIKYGGKL 235 C+G L V+ + ++P+ A + +D + LG++L + + L + G Sbjct: 274 CKGVLKPDVIFFGDNIPKERATQAMEVAKQSDAFLVLGSSLMTMSAFRLCRAAHEAGAMT 333 Query: 236 VICNLQPTKHDNKADLLINYYVDDVLEKVMDILGIEIPS 274 I N+ T+ D+ L IN V ++L +V+D+ + +P+ Sbjct: 334 AIVNIGETRADDIVPLKINARVGEILHRVLDVGSLSVPA 372 >At5g09230.1 68418.m01050 transcriptional regulator Sir2 family protein contains Pfam domain PF02146: transcriptional regulator, Sir2 family; Length = 373 Score = 50.4 bits (115), Expect = 2e-06 Identities = 39/135 (28%), Positives = 55/135 (40%), Gaps = 27/135 (20%) Query: 37 LAQLVKDSKHIVVHTGAGISTSAGIPDFRGPNGVWTLEKEGKKPTINVS----------- 85 L +L + S + + TGAG+ST GIPD+R PNG ++ G KP + Sbjct: 85 LYRLFEQSSRLTILTGAGVSTECGIPDYRSPNGAYS---SGFKPITHQEFTRSSRARRRY 141 Query: 86 ----------FADAQPTKTHMXXXXXXXXXXXXXXVSQNIDGLHLKSGVPRKFLAELHGN 135 F AQP H ++QN+D LH ++G ELHG Sbjct: 142 WARSYAGWRRFTAAQPGPAHTALASLEKAGRINFMITQNVDRLHHRAGSDP---LELHGT 198 Query: 136 MFIDECNICKRQFVR 150 ++ C C F R Sbjct: 199 VYTVMCLECGFSFPR 213 Score = 41.9 bits (94), Expect = 7e-04 Identities = 24/99 (24%), Positives = 49/99 (49%) Query: 176 CRGRLYDGVLDWEHSLPENDLLMAEWHSSIADLSICLGTTLQIVPSGNLPLETIKYGGKL 235 C+G L V+ + ++P+ A + +D + LG++L + + L + G Sbjct: 274 CKGVLKPDVIFFGDNIPKERATQAMEVAKQSDAFLVLGSSLMTMSAFRLCRAAHEAGAMT 333 Query: 236 VICNLQPTKHDNKADLLINYYVDDVLEKVMDILGIEIPS 274 I N+ T+ D+ L IN V ++L +V+D+ + +P+ Sbjct: 334 AIVNIGETRADDIVPLKINARVGEILHRVLDVGSLSVPA 372 >At5g09230.6 68418.m01054 transcriptional regulator Sir2 family protein contains Pfam domain PF02146: transcriptional regulator, Sir2 family; Length = 271 Score = 41.9 bits (94), Expect = 7e-04 Identities = 24/99 (24%), Positives = 49/99 (49%) Query: 176 CRGRLYDGVLDWEHSLPENDLLMAEWHSSIADLSICLGTTLQIVPSGNLPLETIKYGGKL 235 C+G L V+ + ++P+ A + +D + LG++L + + L + G Sbjct: 172 CKGVLKPDVIFFGDNIPKERATQAMEVAKQSDAFLVLGSSLMTMSAFRLCRAAHEAGAMT 231 Query: 236 VICNLQPTKHDNKADLLINYYVDDVLEKVMDILGIEIPS 274 I N+ T+ D+ L IN V ++L +V+D+ + +P+ Sbjct: 232 AIVNIGETRADDIVPLKINARVGEILHRVLDVGSLSVPA 270 Score = 32.3 bits (70), Expect = 0.53 Identities = 19/65 (29%), Positives = 26/65 (40%), Gaps = 3/65 (4%) Query: 86 FADAQPTKTHMXXXXXXXXXXXXXXVSQNIDGLHLKSGVPRKFLAELHGNMFIDECNICK 145 F AQP H ++QN+D LH ++G ELHG ++ C C Sbjct: 50 FTAAQPGPAHTALASLEKAGRINFMITQNVDRLHHRAGSDP---LELHGTVYTVMCLECG 106 Query: 146 RQFVR 150 F R Sbjct: 107 FSFPR 111 >At5g09230.4 68418.m01053 transcriptional regulator Sir2 family protein contains Pfam domain PF02146: transcriptional regulator, Sir2 family; Length = 271 Score = 41.9 bits (94), Expect = 7e-04 Identities = 24/99 (24%), Positives = 49/99 (49%) Query: 176 CRGRLYDGVLDWEHSLPENDLLMAEWHSSIADLSICLGTTLQIVPSGNLPLETIKYGGKL 235 C+G L V+ + ++P+ A + +D + LG++L + + L + G Sbjct: 172 CKGVLKPDVIFFGDNIPKERATQAMEVAKQSDAFLVLGSSLMTMSAFRLCRAAHEAGAMT 231 Query: 236 VICNLQPTKHDNKADLLINYYVDDVLEKVMDILGIEIPS 274 I N+ T+ D+ L IN V ++L +V+D+ + +P+ Sbjct: 232 AIVNIGETRADDIVPLKINARVGEILHRVLDVGSLSVPA 270 Score = 32.3 bits (70), Expect = 0.53 Identities = 19/65 (29%), Positives = 26/65 (40%), Gaps = 3/65 (4%) Query: 86 FADAQPTKTHMXXXXXXXXXXXXXXVSQNIDGLHLKSGVPRKFLAELHGNMFIDECNICK 145 F AQP H ++QN+D LH ++G ELHG ++ C C Sbjct: 50 FTAAQPGPAHTALASLEKAGRINFMITQNVDRLHHRAGSDP---LELHGTVYTVMCLECG 106 Query: 146 RQFVR 150 F R Sbjct: 107 FSFPR 111 >At5g48690.1 68418.m06025 hypothetical protein Length = 301 Score = 33.9 bits (74), Expect = 0.17 Identities = 25/85 (29%), Positives = 44/85 (51%), Gaps = 6/85 (7%) Query: 286 ETAIIDWSIDRKDVLALEKTFKSKCKGVKKKRILIKTKRFTSNANDIEKSKMIKLEVKEE 345 ET I +RK +AL K K + K ++K I++K NA+ +E+ + + L + E Sbjct: 100 ETKRIAEENERKRNIALRKAEKDEEKKAREK-IMLKV-----NADKLERKRRLGLPTETE 153 Query: 346 NETKDWPLKPSDQEFIIDQKGSIVS 370 + + P+ P D + I+ S+VS Sbjct: 154 SASTSNPVSPLDPKRIVMSSPSVVS 178 >At3g55340.1 68416.m06146 RNA recognition motif (RRM)-containing protein low similarity to nucleolar phosphoprotein (Nopp52), Tetrahymena thermophila, EMBL:TT51555; contains InterPro entry IPR000504: RNA-binding region RNP-1 (RNA recognition motif) (RRM) Length = 597 Score = 32.7 bits (71), Expect = 0.40 Identities = 18/48 (37%), Positives = 27/48 (56%) Query: 303 EKTFKSKCKGVKKKRILIKTKRFTSNANDIEKSKMIKLEVKEENETKD 350 +K K K K KKKR + KT + N EK K+ ++EV +N+ +D Sbjct: 109 QKPQKKKNKKKKKKRKVNKTPKKAEEGNVEEKVKVEEIEVNTDNKEED 156 >At3g48970.1 68416.m05349 copper-binding family protein similar to copper homeostasis factor gi:3168840 from Arabidopsis thaliana; contains Pfam heavy-metal-associated domain PF00403 Length = 140 Score = 29.9 bits (64), Expect = 2.8 Identities = 15/59 (25%), Positives = 31/59 (52%), Gaps = 1/59 (1%) Query: 302 LEKTFKSKCKGVKKKRILIKTKRFTSNANDIEKSKMIKLEVKEENETKDWPLKPSDQEF 360 L KT K KGV++ + ++T++ T+ +E+ K++K + + WP + + F Sbjct: 21 LRKTLL-KLKGVEEVEVEMETQKVTARGYRLEEKKVLKAVRRAGKAAELWPYRLGNSHF 78 >At4g14020.1 68417.m02167 rapid alkalinization factor (RALF) family protein Length = 111 Score = 29.5 bits (63), Expect = 3.8 Identities = 15/43 (34%), Positives = 25/43 (58%), Gaps = 3/43 (6%) Query: 323 KRFTSNANDIEKSKMIKLEVKEENETKDWPLKPSDQEFIIDQK 365 +R+ S+ D E +K+I +KE+ ETKD PS + ++ K Sbjct: 27 ERYESDEEDGEVNKLI---LKEDEETKDMVTSPSGERMMLTSK 66 >At3g55960.1 68416.m06218 NLI interacting factor (NIF) family protein contains Pfam profile PF03031: NLI interacting factor Length = 305 Score = 29.5 bits (63), Expect = 3.8 Identities = 16/54 (29%), Positives = 27/54 (50%), Gaps = 1/54 (1%) Query: 142 NICKRQFVRSSPVETVGKKCSGVPCAAHHVTGRPCRGRLYDGVLDWEHSLPEND 195 N+C+ V ++P + + +G+PC A G+P +L D +L L E D Sbjct: 225 NMCRTVIVDNNPFSFLLQPSNGIPCIAFS-AGQPNDTQLLDVILPLLKQLSEED 277 >At2g46070.1 68415.m05731 mitogen-activated protein kinase, putative / MAPK, putative (MPK12) mitogen-activated protein kinase (MAPK)(AtMPK12), PMID:12119167 Length = 372 Score = 29.5 bits (63), Expect = 3.8 Identities = 17/58 (29%), Positives = 29/58 (50%), Gaps = 5/58 (8%) Query: 153 PVETVGKKCSGVPCAA-HHVTGRPCR----GRLYDGVLDWEHSLPENDLLMAEWHSSI 205 P+ +G+ G+ CAA + VTG G +D ++D + +L E LL H ++ Sbjct: 43 PIRPIGRGACGIVCAAVNSVTGEKVAIKKIGNAFDNIIDAKRTLREIKLLRHMDHENV 100 >At3g28770.1 68416.m03591 expressed protein Length = 2081 Score = 29.1 bits (62), Expect = 5.0 Identities = 22/84 (26%), Positives = 44/84 (52%), Gaps = 2/84 (2%) Query: 277 ESENPMKFAETAIIDWSIDRKDVLALEKTFKSKCKGVKKKRILIKTKRFTSNANDIEKSK 336 + EN K +ET I+ S +K+ + ++ SK + KK++ + +++ N+ ++ K Sbjct: 1156 KKENEEK-SETKEIESSKSQKNEVDKKEKKSSKDQQKKKEKEMKESEEKKLKKNEEDRKK 1214 Query: 337 MIKLEV-KEENETKDWPLKPSDQE 359 +E K++ ETK KP D + Sbjct: 1215 QTSVEENKKQKETKKEKNKPKDDK 1238 >At5g07270.1 68418.m00829 ankyrin repeat family protein contains ankyrin repeats, Pfam:PF00023 Length = 513 Score = 28.7 bits (61), Expect = 6.6 Identities = 13/58 (22%), Positives = 29/58 (50%) Query: 197 LMAEWHSSIADLSICLGTTLQIVPSGNLPLETIKYGGKLVICNLQPTKHDNKADLLIN 254 L+ + ++++ ++ GT++ ++ +G+ PL GG L C + + K L N Sbjct: 190 LLLDLEANVSAVTFHYGTSMDMIGAGSTPLHYAACGGNLKCCQILLARGARKMTLNCN 247 >At1g42580.1 68414.m04910 hypothetical protein contains similarity to hypothetical proteins Length = 134 Score = 28.7 bits (61), Expect = 6.6 Identities = 16/50 (32%), Positives = 25/50 (50%), Gaps = 4/50 (8%) Query: 312 GVKKKRILIKTKRFTSNANDIEKSKMIKLEVKE--ENETKDWPLKPSDQE 359 GVK + K K+ S +E + LE++ E + KDW L+ D+E Sbjct: 62 GVKASKA--KAKKVVSKTTTVEDKGNVMLEIQSIGEIKQKDWELRQKDRE 109 >At5g05440.1 68418.m00586 expressed protein low similarity to cytokinin-specific binding protein [Vigna radiata] GI:4190976 Length = 203 Score = 28.3 bits (60), Expect = 8.7 Identities = 17/58 (29%), Positives = 31/58 (53%), Gaps = 1/58 (1%) Query: 184 VLDWEHSLPENDLLMAEWHSSIADLSICLGTTLQIVPSGNLPLETIKYGGKLVICNLQ 241 V+ +H L +N + H+S + ++ + + + VP GN ET+ + +V CNLQ Sbjct: 138 VVGGDHRL-KNYRSVTTLHASDDEGTVVVESYIVDVPPGNTEEETLSFVDTIVRCNLQ 194 Database: arabidopsis Posted date: Oct 3, 2007 3:31 PM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.316 0.135 0.404 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 9,215,418 Number of Sequences: 28952 Number of extensions: 402387 Number of successful extensions: 1001 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 11 Number of HSP's successfully gapped in prelim test: 6 Number of HSP's that attempted gapping in prelim test: 979 Number of HSP's gapped (non-prelim): 29 length of query: 370 length of database: 12,070,560 effective HSP length: 82 effective length of query: 288 effective length of database: 9,696,496 effective search space: 2792590848 effective search space used: 2792590848 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.6 bits) S2: 60 (28.3 bits)
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