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Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA001401-TA|BGIBMGA001401-PA|IPR003000|Silent information
regulator protein Sir2
         (370 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g55760.1 68418.m06950 transcriptional regulator Sir2 family p...   256   2e-68
At5g09230.5 68418.m01052 transcriptional regulator Sir2 family p...    50   2e-06
At5g09230.3 68418.m01049 transcriptional regulator Sir2 family p...    50   2e-06
At5g09230.2 68418.m01051 transcriptional regulator Sir2 family p...    50   2e-06
At5g09230.1 68418.m01050 transcriptional regulator Sir2 family p...    50   2e-06
At5g09230.6 68418.m01054 transcriptional regulator Sir2 family p...    42   7e-04
At5g09230.4 68418.m01053 transcriptional regulator Sir2 family p...    42   7e-04
At5g48690.1 68418.m06025 hypothetical protein                          34   0.17 
At3g55340.1 68416.m06146 RNA recognition motif (RRM)-containing ...    33   0.40 
At3g48970.1 68416.m05349 copper-binding family protein similar t...    30   2.8  
At4g14020.1 68417.m02167 rapid alkalinization factor (RALF) fami...    29   3.8  
At3g55960.1 68416.m06218 NLI interacting factor (NIF) family pro...    29   3.8  
At2g46070.1 68415.m05731 mitogen-activated protein kinase, putat...    29   3.8  
At3g28770.1 68416.m03591 expressed protein                             29   5.0  
At5g07270.1 68418.m00829 ankyrin repeat family protein contains ...    29   6.6  
At1g42580.1 68414.m04910 hypothetical protein contains similarit...    29   6.6  
At5g05440.1 68418.m00586 expressed protein low similarity to cyt...    28   8.7  

>At5g55760.1 68418.m06950 transcriptional regulator Sir2 family
           protein contains Pfam domain PF02146: transcriptional
           regulator, Sir2 family
          Length = 473

 Score =  256 bits (626), Expect = 2e-68
 Identities = 131/276 (47%), Positives = 178/276 (64%), Gaps = 6/276 (2%)

Query: 1   MSCNYAEGLSPYENKGILGVPEKFDSNDKLNQKCVLLAQLVKDSKHIVVHTGAGISTSAG 60
           MS  YAE LS  E+ G +G+ E FD +  L  K   LA+L++ SKH+VV TGAGISTS G
Sbjct: 1   MSLGYAEKLSFIEDVGQVGMAEFFDPSHLLQCKIEELAKLIQKSKHLVVFTGAGISTSCG 60

Query: 61  IPDFRGPNGVWTLEKEGKK-PTINVSFADAQPTKTHMXXXXXXXXXXXXXXVSQNIDGLH 119
           IPDFRGP G+WTL++EGK  P  ++ F  A P+ THM              +SQN+DGLH
Sbjct: 61  IPDFRGPKGIWTLQREGKDLPKASLPFHRAMPSMTHMALVELERAGILKFVISQNVDGLH 120

Query: 120 LKSGVPRKFLAELHGNMFIDECNICKRQFVRSSPVETVGKKCSGVPCAAHHVTGRPCRGR 179
           L+SG+PR+ L+ELHG+ F++ C  C  +++R   VET+G K +   C+        C  +
Sbjct: 121 LRSGIPREKLSELHGDSFMEMCPSCGAEYLRDFEVETIGLKETSRKCSVEK-----CGAK 175

Query: 180 LYDGVLDWEHSLPENDLLMAEWHSSIADLSICLGTTLQIVPSGNLPLETIKYGGKLVICN 239
           L D VLDWE +LP  ++  AE H   ADL +CLGT+LQI P+ NLPL+ +K GGK+VI N
Sbjct: 176 LKDTVLDWEDALPPKEIDPAEKHCKKADLVLCLGTSLQITPACNLPLKCLKGGGKIVIVN 235

Query: 240 LQPTKHDNKADLLINYYVDDVLEKVMDILGIEIPSY 275
           LQ T  D KA+++I+  VD V+  VM+ L ++IP Y
Sbjct: 236 LQKTPKDKKANVVIHGLVDKVVAGVMESLNMKIPPY 271


>At5g09230.5 68418.m01052 transcriptional regulator Sir2 family
           protein contains Pfam domain PF02146: transcriptional
           regulator, Sir2 family;
          Length = 354

 Score = 50.4 bits (115), Expect = 2e-06
 Identities = 39/135 (28%), Positives = 55/135 (40%), Gaps = 27/135 (20%)

Query: 37  LAQLVKDSKHIVVHTGAGISTSAGIPDFRGPNGVWTLEKEGKKPTINVS----------- 85
           L +L + S  + + TGAG+ST  GIPD+R PNG ++    G KP  +             
Sbjct: 66  LYRLFEQSSRLTILTGAGVSTECGIPDYRSPNGAYS---SGFKPITHQEFTRSSRARRRY 122

Query: 86  ----------FADAQPTKTHMXXXXXXXXXXXXXXVSQNIDGLHLKSGVPRKFLAELHGN 135
                     F  AQP   H               ++QN+D LH ++G       ELHG 
Sbjct: 123 WARSYAGWRRFTAAQPGPAHTALASLEKAGRINFMITQNVDRLHHRAGSDP---LELHGT 179

Query: 136 MFIDECNICKRQFVR 150
           ++   C  C   F R
Sbjct: 180 VYTVMCLECGFSFPR 194



 Score = 41.9 bits (94), Expect = 7e-04
 Identities = 24/99 (24%), Positives = 49/99 (49%)

Query: 176 CRGRLYDGVLDWEHSLPENDLLMAEWHSSIADLSICLGTTLQIVPSGNLPLETIKYGGKL 235
           C+G L   V+ +  ++P+     A   +  +D  + LG++L  + +  L     + G   
Sbjct: 255 CKGVLKPDVIFFGDNIPKERATQAMEVAKQSDAFLVLGSSLMTMSAFRLCRAAHEAGAMT 314

Query: 236 VICNLQPTKHDNKADLLINYYVDDVLEKVMDILGIEIPS 274
            I N+  T+ D+   L IN  V ++L +V+D+  + +P+
Sbjct: 315 AIVNIGETRADDIVPLKINARVGEILHRVLDVGSLSVPA 353


>At5g09230.3 68418.m01049 transcriptional regulator Sir2 family
           protein contains Pfam domain PF02146: transcriptional
           regulator, Sir2 family;
          Length = 324

 Score = 50.4 bits (115), Expect = 2e-06
 Identities = 39/135 (28%), Positives = 55/135 (40%), Gaps = 27/135 (20%)

Query: 37  LAQLVKDSKHIVVHTGAGISTSAGIPDFRGPNGVWTLEKEGKKPTINVS----------- 85
           L +L + S  + + TGAG+ST  GIPD+R PNG ++    G KP  +             
Sbjct: 85  LYRLFEQSSRLTILTGAGVSTECGIPDYRSPNGAYS---SGFKPITHQEFTRSSRARRRY 141

Query: 86  ----------FADAQPTKTHMXXXXXXXXXXXXXXVSQNIDGLHLKSGVPRKFLAELHGN 135
                     F  AQP   H               ++QN+D LH ++G       ELHG 
Sbjct: 142 WARSYAGWRRFTAAQPGPAHTALASLEKAGRINFMITQNVDRLHHRAGSDP---LELHGT 198

Query: 136 MFIDECNICKRQFVR 150
           ++   C  C   F R
Sbjct: 199 VYTVMCLECGFSFPR 213


>At5g09230.2 68418.m01051 transcriptional regulator Sir2 family
           protein contains Pfam domain PF02146: transcriptional
           regulator, Sir2 family;
          Length = 373

 Score = 50.4 bits (115), Expect = 2e-06
 Identities = 39/135 (28%), Positives = 55/135 (40%), Gaps = 27/135 (20%)

Query: 37  LAQLVKDSKHIVVHTGAGISTSAGIPDFRGPNGVWTLEKEGKKPTINVS----------- 85
           L +L + S  + + TGAG+ST  GIPD+R PNG ++    G KP  +             
Sbjct: 85  LYRLFEQSSRLTILTGAGVSTECGIPDYRSPNGAYS---SGFKPITHQEFTRSSRARRRY 141

Query: 86  ----------FADAQPTKTHMXXXXXXXXXXXXXXVSQNIDGLHLKSGVPRKFLAELHGN 135
                     F  AQP   H               ++QN+D LH ++G       ELHG 
Sbjct: 142 WARSYAGWRRFTAAQPGPAHTALASLEKAGRINFMITQNVDRLHHRAGSDP---LELHGT 198

Query: 136 MFIDECNICKRQFVR 150
           ++   C  C   F R
Sbjct: 199 VYTVMCLECGFSFPR 213



 Score = 41.9 bits (94), Expect = 7e-04
 Identities = 24/99 (24%), Positives = 49/99 (49%)

Query: 176 CRGRLYDGVLDWEHSLPENDLLMAEWHSSIADLSICLGTTLQIVPSGNLPLETIKYGGKL 235
           C+G L   V+ +  ++P+     A   +  +D  + LG++L  + +  L     + G   
Sbjct: 274 CKGVLKPDVIFFGDNIPKERATQAMEVAKQSDAFLVLGSSLMTMSAFRLCRAAHEAGAMT 333

Query: 236 VICNLQPTKHDNKADLLINYYVDDVLEKVMDILGIEIPS 274
            I N+  T+ D+   L IN  V ++L +V+D+  + +P+
Sbjct: 334 AIVNIGETRADDIVPLKINARVGEILHRVLDVGSLSVPA 372


>At5g09230.1 68418.m01050 transcriptional regulator Sir2 family
           protein contains Pfam domain PF02146: transcriptional
           regulator, Sir2 family;
          Length = 373

 Score = 50.4 bits (115), Expect = 2e-06
 Identities = 39/135 (28%), Positives = 55/135 (40%), Gaps = 27/135 (20%)

Query: 37  LAQLVKDSKHIVVHTGAGISTSAGIPDFRGPNGVWTLEKEGKKPTINVS----------- 85
           L +L + S  + + TGAG+ST  GIPD+R PNG ++    G KP  +             
Sbjct: 85  LYRLFEQSSRLTILTGAGVSTECGIPDYRSPNGAYS---SGFKPITHQEFTRSSRARRRY 141

Query: 86  ----------FADAQPTKTHMXXXXXXXXXXXXXXVSQNIDGLHLKSGVPRKFLAELHGN 135
                     F  AQP   H               ++QN+D LH ++G       ELHG 
Sbjct: 142 WARSYAGWRRFTAAQPGPAHTALASLEKAGRINFMITQNVDRLHHRAGSDP---LELHGT 198

Query: 136 MFIDECNICKRQFVR 150
           ++   C  C   F R
Sbjct: 199 VYTVMCLECGFSFPR 213



 Score = 41.9 bits (94), Expect = 7e-04
 Identities = 24/99 (24%), Positives = 49/99 (49%)

Query: 176 CRGRLYDGVLDWEHSLPENDLLMAEWHSSIADLSICLGTTLQIVPSGNLPLETIKYGGKL 235
           C+G L   V+ +  ++P+     A   +  +D  + LG++L  + +  L     + G   
Sbjct: 274 CKGVLKPDVIFFGDNIPKERATQAMEVAKQSDAFLVLGSSLMTMSAFRLCRAAHEAGAMT 333

Query: 236 VICNLQPTKHDNKADLLINYYVDDVLEKVMDILGIEIPS 274
            I N+  T+ D+   L IN  V ++L +V+D+  + +P+
Sbjct: 334 AIVNIGETRADDIVPLKINARVGEILHRVLDVGSLSVPA 372


>At5g09230.6 68418.m01054 transcriptional regulator Sir2 family
           protein contains Pfam domain PF02146: transcriptional
           regulator, Sir2 family;
          Length = 271

 Score = 41.9 bits (94), Expect = 7e-04
 Identities = 24/99 (24%), Positives = 49/99 (49%)

Query: 176 CRGRLYDGVLDWEHSLPENDLLMAEWHSSIADLSICLGTTLQIVPSGNLPLETIKYGGKL 235
           C+G L   V+ +  ++P+     A   +  +D  + LG++L  + +  L     + G   
Sbjct: 172 CKGVLKPDVIFFGDNIPKERATQAMEVAKQSDAFLVLGSSLMTMSAFRLCRAAHEAGAMT 231

Query: 236 VICNLQPTKHDNKADLLINYYVDDVLEKVMDILGIEIPS 274
            I N+  T+ D+   L IN  V ++L +V+D+  + +P+
Sbjct: 232 AIVNIGETRADDIVPLKINARVGEILHRVLDVGSLSVPA 270



 Score = 32.3 bits (70), Expect = 0.53
 Identities = 19/65 (29%), Positives = 26/65 (40%), Gaps = 3/65 (4%)

Query: 86  FADAQPTKTHMXXXXXXXXXXXXXXVSQNIDGLHLKSGVPRKFLAELHGNMFIDECNICK 145
           F  AQP   H               ++QN+D LH ++G       ELHG ++   C  C 
Sbjct: 50  FTAAQPGPAHTALASLEKAGRINFMITQNVDRLHHRAGSDP---LELHGTVYTVMCLECG 106

Query: 146 RQFVR 150
             F R
Sbjct: 107 FSFPR 111


>At5g09230.4 68418.m01053 transcriptional regulator Sir2 family
           protein contains Pfam domain PF02146: transcriptional
           regulator, Sir2 family;
          Length = 271

 Score = 41.9 bits (94), Expect = 7e-04
 Identities = 24/99 (24%), Positives = 49/99 (49%)

Query: 176 CRGRLYDGVLDWEHSLPENDLLMAEWHSSIADLSICLGTTLQIVPSGNLPLETIKYGGKL 235
           C+G L   V+ +  ++P+     A   +  +D  + LG++L  + +  L     + G   
Sbjct: 172 CKGVLKPDVIFFGDNIPKERATQAMEVAKQSDAFLVLGSSLMTMSAFRLCRAAHEAGAMT 231

Query: 236 VICNLQPTKHDNKADLLINYYVDDVLEKVMDILGIEIPS 274
            I N+  T+ D+   L IN  V ++L +V+D+  + +P+
Sbjct: 232 AIVNIGETRADDIVPLKINARVGEILHRVLDVGSLSVPA 270



 Score = 32.3 bits (70), Expect = 0.53
 Identities = 19/65 (29%), Positives = 26/65 (40%), Gaps = 3/65 (4%)

Query: 86  FADAQPTKTHMXXXXXXXXXXXXXXVSQNIDGLHLKSGVPRKFLAELHGNMFIDECNICK 145
           F  AQP   H               ++QN+D LH ++G       ELHG ++   C  C 
Sbjct: 50  FTAAQPGPAHTALASLEKAGRINFMITQNVDRLHHRAGSDP---LELHGTVYTVMCLECG 106

Query: 146 RQFVR 150
             F R
Sbjct: 107 FSFPR 111


>At5g48690.1 68418.m06025 hypothetical protein
          Length = 301

 Score = 33.9 bits (74), Expect = 0.17
 Identities = 25/85 (29%), Positives = 44/85 (51%), Gaps = 6/85 (7%)

Query: 286 ETAIIDWSIDRKDVLALEKTFKSKCKGVKKKRILIKTKRFTSNANDIEKSKMIKLEVKEE 345
           ET  I    +RK  +AL K  K + K  ++K I++K      NA+ +E+ + + L  + E
Sbjct: 100 ETKRIAEENERKRNIALRKAEKDEEKKAREK-IMLKV-----NADKLERKRRLGLPTETE 153

Query: 346 NETKDWPLKPSDQEFIIDQKGSIVS 370
           + +   P+ P D + I+    S+VS
Sbjct: 154 SASTSNPVSPLDPKRIVMSSPSVVS 178


>At3g55340.1 68416.m06146 RNA recognition motif (RRM)-containing
           protein low similarity to nucleolar phosphoprotein
           (Nopp52), Tetrahymena thermophila, EMBL:TT51555;
           contains InterPro entry IPR000504: RNA-binding region
           RNP-1 (RNA recognition motif) (RRM)
          Length = 597

 Score = 32.7 bits (71), Expect = 0.40
 Identities = 18/48 (37%), Positives = 27/48 (56%)

Query: 303 EKTFKSKCKGVKKKRILIKTKRFTSNANDIEKSKMIKLEVKEENETKD 350
           +K  K K K  KKKR + KT +     N  EK K+ ++EV  +N+ +D
Sbjct: 109 QKPQKKKNKKKKKKRKVNKTPKKAEEGNVEEKVKVEEIEVNTDNKEED 156


>At3g48970.1 68416.m05349 copper-binding family protein similar to
           copper homeostasis factor gi:3168840 from Arabidopsis
           thaliana; contains Pfam heavy-metal-associated domain
           PF00403
          Length = 140

 Score = 29.9 bits (64), Expect = 2.8
 Identities = 15/59 (25%), Positives = 31/59 (52%), Gaps = 1/59 (1%)

Query: 302 LEKTFKSKCKGVKKKRILIKTKRFTSNANDIEKSKMIKLEVKEENETKDWPLKPSDQEF 360
           L KT   K KGV++  + ++T++ T+    +E+ K++K   +     + WP +  +  F
Sbjct: 21  LRKTLL-KLKGVEEVEVEMETQKVTARGYRLEEKKVLKAVRRAGKAAELWPYRLGNSHF 78


>At4g14020.1 68417.m02167 rapid alkalinization factor (RALF) family
           protein
          Length = 111

 Score = 29.5 bits (63), Expect = 3.8
 Identities = 15/43 (34%), Positives = 25/43 (58%), Gaps = 3/43 (6%)

Query: 323 KRFTSNANDIEKSKMIKLEVKEENETKDWPLKPSDQEFIIDQK 365
           +R+ S+  D E +K+I   +KE+ ETKD    PS +  ++  K
Sbjct: 27  ERYESDEEDGEVNKLI---LKEDEETKDMVTSPSGERMMLTSK 66


>At3g55960.1 68416.m06218 NLI interacting factor (NIF) family
           protein contains Pfam profile PF03031: NLI interacting
           factor
          Length = 305

 Score = 29.5 bits (63), Expect = 3.8
 Identities = 16/54 (29%), Positives = 27/54 (50%), Gaps = 1/54 (1%)

Query: 142 NICKRQFVRSSPVETVGKKCSGVPCAAHHVTGRPCRGRLYDGVLDWEHSLPEND 195
           N+C+   V ++P   + +  +G+PC A    G+P   +L D +L     L E D
Sbjct: 225 NMCRTVIVDNNPFSFLLQPSNGIPCIAFS-AGQPNDTQLLDVILPLLKQLSEED 277


>At2g46070.1 68415.m05731 mitogen-activated protein kinase, putative
           / MAPK, putative (MPK12) mitogen-activated protein
           kinase (MAPK)(AtMPK12), PMID:12119167
          Length = 372

 Score = 29.5 bits (63), Expect = 3.8
 Identities = 17/58 (29%), Positives = 29/58 (50%), Gaps = 5/58 (8%)

Query: 153 PVETVGKKCSGVPCAA-HHVTGRPCR----GRLYDGVLDWEHSLPENDLLMAEWHSSI 205
           P+  +G+   G+ CAA + VTG        G  +D ++D + +L E  LL    H ++
Sbjct: 43  PIRPIGRGACGIVCAAVNSVTGEKVAIKKIGNAFDNIIDAKRTLREIKLLRHMDHENV 100


>At3g28770.1 68416.m03591 expressed protein 
          Length = 2081

 Score = 29.1 bits (62), Expect = 5.0
 Identities = 22/84 (26%), Positives = 44/84 (52%), Gaps = 2/84 (2%)

Query: 277  ESENPMKFAETAIIDWSIDRKDVLALEKTFKSKCKGVKKKRILIKTKRFTSNANDIEKSK 336
            + EN  K +ET  I+ S  +K+ +  ++   SK +  KK++ + +++      N+ ++ K
Sbjct: 1156 KKENEEK-SETKEIESSKSQKNEVDKKEKKSSKDQQKKKEKEMKESEEKKLKKNEEDRKK 1214

Query: 337  MIKLEV-KEENETKDWPLKPSDQE 359
               +E  K++ ETK    KP D +
Sbjct: 1215 QTSVEENKKQKETKKEKNKPKDDK 1238


>At5g07270.1 68418.m00829 ankyrin repeat family protein contains
           ankyrin repeats, Pfam:PF00023
          Length = 513

 Score = 28.7 bits (61), Expect = 6.6
 Identities = 13/58 (22%), Positives = 29/58 (50%)

Query: 197 LMAEWHSSIADLSICLGTTLQIVPSGNLPLETIKYGGKLVICNLQPTKHDNKADLLIN 254
           L+ +  ++++ ++   GT++ ++ +G+ PL     GG L  C +   +   K  L  N
Sbjct: 190 LLLDLEANVSAVTFHYGTSMDMIGAGSTPLHYAACGGNLKCCQILLARGARKMTLNCN 247


>At1g42580.1 68414.m04910 hypothetical protein contains similarity
           to hypothetical proteins
          Length = 134

 Score = 28.7 bits (61), Expect = 6.6
 Identities = 16/50 (32%), Positives = 25/50 (50%), Gaps = 4/50 (8%)

Query: 312 GVKKKRILIKTKRFTSNANDIEKSKMIKLEVKE--ENETKDWPLKPSDQE 359
           GVK  +   K K+  S    +E    + LE++   E + KDW L+  D+E
Sbjct: 62  GVKASKA--KAKKVVSKTTTVEDKGNVMLEIQSIGEIKQKDWELRQKDRE 109


>At5g05440.1 68418.m00586 expressed protein low similarity to
           cytokinin-specific binding protein [Vigna radiata]
           GI:4190976
          Length = 203

 Score = 28.3 bits (60), Expect = 8.7
 Identities = 17/58 (29%), Positives = 31/58 (53%), Gaps = 1/58 (1%)

Query: 184 VLDWEHSLPENDLLMAEWHSSIADLSICLGTTLQIVPSGNLPLETIKYGGKLVICNLQ 241
           V+  +H L +N   +   H+S  + ++ + + +  VP GN   ET+ +   +V CNLQ
Sbjct: 138 VVGGDHRL-KNYRSVTTLHASDDEGTVVVESYIVDVPPGNTEEETLSFVDTIVRCNLQ 194


  Database: arabidopsis
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.316    0.135    0.404 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,215,418
Number of Sequences: 28952
Number of extensions: 402387
Number of successful extensions: 1001
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 11
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 979
Number of HSP's gapped (non-prelim): 29
length of query: 370
length of database: 12,070,560
effective HSP length: 82
effective length of query: 288
effective length of database: 9,696,496
effective search space: 2792590848
effective search space used: 2792590848
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)
S2: 60 (28.3 bits)

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