Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA000991-TA|BGIBMGA000991-PA|IPR004154|Anticodon-binding,
IPR009068|S15/NS1, RNA-binding, IPR010987|Glutathione S-transferase,
C-terminal-like, IPR000738|WHEP-TRS
(491 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g02760.1 68416.m00268 histidyl-tRNA synthetase, putative / hi... 174 1e-43
At3g46100.1 68416.m04988 histidyl-tRNA synthetase / histidine--t... 69 5e-12
At2g04842.1 68415.m00498 threonyl-tRNA synthetase, putative / th... 52 9e-07
At3g59410.1 68416.m06626 protein kinase family protein low simil... 50 3e-06
At2g18110.1 68415.m02105 elongation factor 1-beta, putative / EF... 44 3e-04
At1g30230.1 68414.m03698 elongation factor 1-beta / EF-1-beta id... 44 3e-04
At3g62120.2 68416.m06980 tRNA synthetase class II (G, H, P and S... 38 0.020
At3g62120.1 68416.m06979 tRNA synthetase class II (G, H, P and S... 38 0.020
At5g10880.1 68418.m01263 tRNA synthetase-related / tRNA ligase-r... 32 0.99
At1g47900.1 68414.m05334 expressed protein 30 3.0
At4g30170.1 68417.m04290 peroxidase, putative identical to perox... 30 4.0
At3g25520.1 68416.m03173 60S ribosomal protein L5 similar to 60S... 29 7.0
At1g73430.1 68414.m08500 sec34-like family protein contains Pfam... 29 7.0
At4g24170.1 68417.m03468 kinesin motor family protein contains P... 29 9.2
At2g31970.1 68415.m03906 DNA repair-recombination protein (RAD50... 29 9.2
>At3g02760.1 68416.m00268 histidyl-tRNA synthetase, putative /
histidine--tRNA ligase, putative similar to SP|P12081
Histidyl-tRNA synthetase (EC 6.1.1.21) (Histidine--tRNA
ligase) (HisRS) {Homo sapiens}; contains Pfam profiles
PF00587: tRNA synthetase class II core domain (G, H, P,
S and T), PF03129: Anticodon binding domain
Length = 479
Score = 174 bits (423), Expect = 1e-43
Identities = 85/196 (43%), Positives = 124/196 (63%), Gaps = 2/196 (1%)
Query: 169 KIITVFRRHGAECIDTPVFELKDFDIAGIYDPMVPDAECLKVVTEILEALDIGQYILKVN 228
+I V+RR F DFDIAG+++PM PD E +K++TE+L+ L+IG Y +K+N
Sbjct: 137 QIAKVYRRDNPSKGRYREFYQCDFDIAGLFEPMGPDFEIVKILTELLDELEIGDYEVKLN 196
Query: 229 HRRLLDGMFEACGVPADKFRTTCSTVDKLDKSPWEEVRTEMINEKGVSPDAADRIGEYGR 288
HR+LLDGM E CGVP +KFRT CS++DKLDK +E+V+ EM+ EKG+S + ADRIG + +
Sbjct: 197 HRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFEQVKKEMVEEKGLSSEIADRIGNFVK 256
Query: 289 LNGS--TXXXXXXXXXXXXXXXXAALEGVEAIKTLLQYCELFGIKDKILFDLSLARGLDY 346
G+ ++ E ++ + + + + ++I+FDLSLARGLDY
Sbjct: 257 EKGAPLELLSKLRQEGSEFLDNQSSREALDELSIMFEALKRSKCSERIVFDLSLARGLDY 316
Query: 347 YTGVIYEAVLTRVSIG 362
YTGVI+EAV +G
Sbjct: 317 YTGVIFEAVCIGAEVG 332
Score = 57.6 bits (133), Expect = 2e-08
Identities = 24/45 (53%), Positives = 31/45 (68%)
Query: 147 KTPKGTRDYNPQQMTIRNNVLDKIITVFRRHGAECIDTPVFELKD 191
K PKGTRD+ +QM +R I VF+RHGA +DTPVFEL++
Sbjct: 43 KLPKGTRDFAKEQMAVREKAFSIIQNVFKRHGATALDTPVFELRE 87
Score = 54.8 bits (126), Expect = 1e-07
Identities = 32/123 (26%), Positives = 62/123 (50%), Gaps = 6/123 (4%)
Query: 359 VSIGVERIFSVLEAKLAAGDITVRTSDIDVYVASAQKNFLEERMKICAELWDAGIKTEQS 418
+S+G+ER+F+++E +R ++ V V+ N L E ++ ++LW A I E
Sbjct: 357 MSLGIERVFNIMEELNEKQKQVIRPTETQVLVSIMVDNKLAEAAELVSQLWGAKINAE-- 414
Query: 419 YKKNPKMLNQLQHCEENKIPLAVVLGDSELKRGVVKIRNI----TTREEDEVPRNDLVQE 474
Y + + +E+ IP V++G+ EL V ++ + +E+ R+ V+E
Sbjct: 415 YLVSKRKEKHFNRAKESGIPWMVMVGEKELSGSFVTLKKLEKGSEEKEDQTCTRDRFVEE 474
Query: 475 IKK 477
+KK
Sbjct: 475 LKK 477
>At3g46100.1 68416.m04988 histidyl-tRNA synthetase / histidine--tRNA
ligase identical to histidyl-tRNA synthetase
[Arabidopsis thaliana] GI:3659909
Length = 486
Score = 69.3 bits (162), Expect = 5e-12
Identities = 45/165 (27%), Positives = 76/165 (46%), Gaps = 13/165 (7%)
Query: 191 DFDIAGIYDPMVPDAECLKVVTEILEALDI--GQYILKVNHRRLLDGMFEACGVPADKFR 248
+ DI G+ + +AE + + + + I KV+ R++L + + GVP D F
Sbjct: 190 NMDIIGV-PQVTAEAELISSIVTFFKRIGITASDVGFKVSSRKVLQELLKKYGVPEDLFG 248
Query: 249 TTCSTVDKLDKSPWEEVRTEMINEKGVSPDAADRIGEYGRLNGSTXXXXXXXXXXXXXXX 308
C +DK++K P +E++ E + G+S DA +++
Sbjct: 249 RVCIIIDKIEKIPIDEIKKE-LGFTGISEDAIEQL---------LQVLSVKSLDDLEDII 298
Query: 309 XAALEGVEAIKTLLQYCELFGIKDKILFDLSLARGLDYYTGVIYE 353
A E + +K L E FG + I FD S+ RGL YYTG+++E
Sbjct: 299 GGAGEAIADLKQLFSLAEKFGYSEWIQFDASVVRGLAYYTGIVFE 343
Score = 48.8 bits (111), Expect = 8e-06
Identities = 24/60 (40%), Positives = 36/60 (60%)
Query: 129 ALKAQLVTEDAGPQKFTLKTPKGTRDYNPQQMTIRNNVLDKIITVFRRHGAECIDTPVFE 188
++ A LVTE+ +K + PKGTRD+ P+ M +RN + + V R +G E +D PV E
Sbjct: 52 SIVAPLVTEEDFHKKIDVNPPKGTRDFPPEDMRLRNWLFNHFKEVSRLYGYEEVDYPVLE 111
>At2g04842.1 68415.m00498 threonyl-tRNA synthetase, putative /
threonine--tRNA ligase, putative similar to SP|P18256
Threonyl-tRNA synthetase 2 (EC 6.1.1.3) (Threonine--tRNA
ligase) (ThrRS) {Bacillus subtilis}; contains Pfam
profiles PF00587: tRNA synthetase class II core domain
(G, H, P, S and T), PF03129: Anticodon binding domain
Length = 650
Score = 52.0 bits (119), Expect = 9e-07
Identities = 37/128 (28%), Positives = 67/128 (52%), Gaps = 6/128 (4%)
Query: 355 VLTRVSIG-VERIFSVLEAKLAAGDITVRTSDIDVYVASAQKNFLEERMKICAELWDAGI 413
++ R +G +ER F VL + AGD + S + V V N LE ++ +L G+
Sbjct: 522 MIHRAVLGSLERFFGVL-IEHYAGDFPLWLSPVQVRVLPVTDNQLEFCKEVSKKLRACGV 580
Query: 414 KTEQSY-KKNPKMLNQLQHCEENKIPLAVVLGDSELKRGVVKIRNITTREEDEVPRNDLV 472
+ E + ++ PK++ ++ E KIPL V+G E++ G V +R+ E +P +DL+
Sbjct: 581 RAELCHGERLPKLI---RNAETQKIPLMAVVGPKEVETGTVTVRSRFGGELGTIPVDDLI 637
Query: 473 QEIKKRID 480
+I ++
Sbjct: 638 NKINIAVE 645
>At3g59410.1 68416.m06626 protein kinase family protein low similarity
to GCN2 eIF2alpha kinase [Mus musculus] GI:6066585;
contains Pfam profiles PF03129: Anticodon binding domain,
PF00069: Protein kinase domain
Length = 1241
Score = 50.4 bits (115), Expect = 3e-06
Identities = 37/119 (31%), Positives = 61/119 (51%), Gaps = 7/119 (5%)
Query: 359 VSIGVERIFSVLEAKLAAGDITVRTSDIDVYVAS-AQKNFLEERMKICAELWDAGIKTEQ 417
VS+ +E IF L L V TS V V S L +RM++ AELW+ IK E
Sbjct: 1109 VSLALETIFQHLPMDLRPIRNEVSTS---VLVCSRGGGGLLVQRMELVAELWEKSIKAEF 1165
Query: 418 SYKKNPKMLNQLQHCEENKIPLAVVLGDSELKRG---VVKIRNITTREEDEVPRNDLVQ 473
+P + Q ++ E++I V++ +S + + VK+R++ ++E V R +LV+
Sbjct: 1166 VPTPDPSLTEQYEYANEHEIKCLVIITESGVAQNQIEFVKVRHLELKKEKVVGREELVK 1224
Score = 37.9 bits (84), Expect = 0.015
Identities = 38/200 (19%), Positives = 77/200 (38%), Gaps = 12/200 (6%)
Query: 169 KIITVFRRHGAECIDTPVFELKDFDIAGIYDPMVPDAECLKVVTEILEAL-DIGQYILKV 227
+I V+RR P + DFDI G + +AE LKV+ +I + G + +
Sbjct: 871 EISHVYRRAIGHSPPNPCLQA-DFDIVG-GTLSLTEAEVLKVIVDITTHIFHRGSCDIHL 928
Query: 228 NHRRLLDGMFEACGVPADKFRTTCSTVDKLD---------KSPWEEVRTEMINEKGVSPD 278
NH LLD ++ G+ A+ R + + K W +R +++ E +
Sbjct: 929 NHGDLLDAIWSWAGIKAEHRRKVAELLSMMGSLRPQSSERKLKWVFIRRQLLQELKLPEA 988
Query: 279 AADRIGEYGRLNGSTXXXXXXXXXXXXXXXXAALEGVEAIKTLLQYCELFGIKDKILFDL 338
+R+ + ++ + LL Y ++ I++ + D+
Sbjct: 989 VVNRLQTVASRFCGDADQALPRLRGALRADRPTRKALDELSNLLTYLRVWRIEEHVHIDV 1048
Query: 339 SLARGLDYYTGVIYEAVLTR 358
+ Y+ + ++ LT+
Sbjct: 1049 LMPPTESYHRNLFFQVFLTK 1068
>At2g18110.1 68415.m02105 elongation factor 1-beta, putative /
EF-1-beta, putative nearly identical to eEF-1beta
[Arabidopsis thaliana] GI:398606
Length = 231
Score = 43.6 bits (98), Expect = 3e-04
Identities = 19/47 (40%), Positives = 28/47 (59%)
Query: 42 EEIDKHLSHYSYICGYVPSKPDVDVAKALKSLDFSNYKYIKRWWNHM 88
+++D+HL SYI GY SK D+ V AL S + + RW+NH+
Sbjct: 14 KKLDEHLLTRSYITGYQASKDDITVFTALSKPPTSEFVNVSRWFNHI 60
>At1g30230.1 68414.m03698 elongation factor 1-beta / EF-1-beta
identical to SP|P48006 Elongation factor 1-beta
(EF-1-beta) {Arabidopsis thaliana}
Length = 231
Score = 43.6 bits (98), Expect = 3e-04
Identities = 20/47 (42%), Positives = 27/47 (57%)
Query: 42 EEIDKHLSHYSYICGYVPSKPDVDVAKALKSLDFSNYKYIKRWWNHM 88
+++D+HL SYI GY SK D+ V AL S Y RW+NH+
Sbjct: 14 KKLDEHLLTRSYITGYQASKDDITVFAALAKPPTSQYVNASRWYNHI 60
>At3g62120.2 68416.m06980 tRNA synthetase class II (G, H, P and S)
family protein similar to SP|P07814 Bifunctional
aminoacyl-tRNA synthetase [Includes: Glutamyl-tRNA
synthetase (EC 6.1.1.17) (Glutamate--tRNA ligase);
Prolyl-tRNA synthetase (EC 6.1.1.15) (Proline--tRNA
ligase)] {Homo sapiens}; contains Pfam profiles PF00587:
tRNA synthetase class II core domain (G, H, P, S and T),
PF03129: Anticodon binding domain
Length = 530
Score = 37.5 bits (83), Expect = 0.020
Identities = 18/78 (23%), Positives = 39/78 (50%)
Query: 408 LWDAGIKTEQSYKKNPKMLNQLQHCEENKIPLAVVLGDSELKRGVVKIRNITTREEDEVP 467
L +AGI+ E+ + N + E +PL + +G +L+ V+ ++++P
Sbjct: 361 LCEAGIRAEEDLRDNYSPGWKYSDWEMKGVPLRIEIGPRDLENDQVRTVRRDNGVKEDIP 420
Query: 468 RNDLVQEIKKRIDNLNLN 485
R LV+ +K+ ++ + N
Sbjct: 421 RGSLVEHVKELLEKIQQN 438
>At3g62120.1 68416.m06979 tRNA synthetase class II (G, H, P and S)
family protein similar to SP|P07814 Bifunctional
aminoacyl-tRNA synthetase [Includes: Glutamyl-tRNA
synthetase (EC 6.1.1.17) (Glutamate--tRNA ligase);
Prolyl-tRNA synthetase (EC 6.1.1.15) (Proline--tRNA
ligase)] {Homo sapiens}; contains Pfam profiles PF00587:
tRNA synthetase class II core domain (G, H, P, S and T),
PF03129: Anticodon binding domain
Length = 530
Score = 37.5 bits (83), Expect = 0.020
Identities = 18/78 (23%), Positives = 39/78 (50%)
Query: 408 LWDAGIKTEQSYKKNPKMLNQLQHCEENKIPLAVVLGDSELKRGVVKIRNITTREEDEVP 467
L +AGI+ E+ + N + E +PL + +G +L+ V+ ++++P
Sbjct: 361 LCEAGIRAEEDLRDNYSPGWKYSDWEMKGVPLRIEIGPRDLENDQVRTVRRDNGVKEDIP 420
Query: 468 RNDLVQEIKKRIDNLNLN 485
R LV+ +K+ ++ + N
Sbjct: 421 RGSLVEHVKELLEKIQQN 438
>At5g10880.1 68418.m01263 tRNA synthetase-related / tRNA
ligase-related similar to SP|P07814 Bifunctional
aminoacyl-tRNA synthetase [Includes: Glutamyl-tRNA
synthetase (EC 6.1.1.17) (Glutamate--tRNA ligase);
Prolyl-tRNA synthetase (EC 6.1.1.15) (Proline--tRNA
ligase)] {Homo sapiens}; contains Pfam profile PF03129:
Anticodon binding domain
Length = 309
Score = 31.9 bits (69), Expect = 0.99
Identities = 19/78 (24%), Positives = 35/78 (44%)
Query: 408 LWDAGIKTEQSYKKNPKMLNQLQHCEENKIPLAVVLGDSELKRGVVKIRNITTREEDEVP 467
L AGI+ E + N + E +PL + G +L V+I + +V
Sbjct: 135 LLGAGIRAEADIRDNYSCGWKYADQELTGVPLRIETGPRDLANDQVRIVTRDNGAKMDVK 194
Query: 468 RNDLVQEIKKRIDNLNLN 485
R DL++++K ++ + N
Sbjct: 195 RGDLIEQVKDLLEKIQSN 212
>At1g47900.1 68414.m05334 expressed protein
Length = 1054
Score = 30.3 bits (65), Expect = 3.0
Identities = 26/98 (26%), Positives = 46/98 (46%), Gaps = 4/98 (4%)
Query: 359 VSIGVERI--FSVLEAKLAAGDITVRTSDIDVYVASAQKNFLEERMKICAELWDAGIKTE 416
V I VE+ F+ LE ++ + D+ ++ D+ V Q EE++K E DA +
Sbjct: 42 VQISVEQYTHFTGLEEQIKSYDVQIKGYDVQVKTYENQVESYEEQVKDFEEQIDAYDEKV 101
Query: 417 QSYKKNPKMLNQLQHCEENKIPLAVVLGDSELKRGVVK 454
Y++ + LN + E+ L+V + K +VK
Sbjct: 102 HEYEEQVQKLN--EDVEDLNEKLSVANEEIVTKEALVK 137
>At4g30170.1 68417.m04290 peroxidase, putative identical to
peroxidase ATP8a [Arabidopsis thaliana]
gi|1546706|emb|CAA67361
Length = 325
Score = 29.9 bits (64), Expect = 4.0
Identities = 27/112 (24%), Positives = 46/112 (41%), Gaps = 5/112 (4%)
Query: 378 DITVRTSDIDVYVASAQKNFLEERMKICAELWDAGIKTEQSYKKNPKMLNQLQHCEENKI 437
D VR D + +AS + + M + + +D +K +Q+ NP N++ C +
Sbjct: 68 DCFVRGCDASIMIASPSERDHPDDMSLAGDGFDTVVKAKQAVDSNPNCRNKVS-CAD--- 123
Query: 438 PLAVVLGDSELKRGVVKIRNITTREEDEVPRNDLVQEIKKRIDNLNLNQLNG 489
LA+ + + G R + + VQ + NLNQLNG
Sbjct: 124 ILALATREVVVLTGGPSYPVELGRRDGRISTKASVQSQLPQ-PEFNLNQLNG 174
>At3g25520.1 68416.m03173 60S ribosomal protein L5 similar to 60S
ribosomal protein L5 GB:P49625 from [Oryza sativa]
Length = 301
Score = 29.1 bits (62), Expect = 7.0
Identities = 23/97 (23%), Positives = 44/97 (45%), Gaps = 11/97 (11%)
Query: 77 NYKYIKRWWNHMRSFSDTEVSLFPDFRQPNFLKYNSKKILESQVKEEVSKLLALKAQLVT 136
NY Y N+M+ + E P+ Q +F Y K + ++E K+ A +
Sbjct: 196 NYIYGGHVSNYMKLLGEDE----PEKLQTHFSAYIKKGVEAESIEELYKKVHAA----IR 247
Query: 137 EDAGPQKFTLKTPKGTRDYNPQQMTI---RNNVLDKI 170
D P+K PK + YN +++T +N +++++
Sbjct: 248 ADPNPKKTVKPAPKQHKRYNLKKLTYEERKNKLIERV 284
>At1g73430.1 68414.m08500 sec34-like family protein contains Pfam
PF04136: Sec34-like family profile; similar to Conserved
oligomeric Golgi complex component 3 (Vesicle docking
protein SEC34 homolog) (p94) (Swiss-Prot:Q96JB2) [Homo
sapiens]
Length = 784
Score = 29.1 bits (62), Expect = 7.0
Identities = 21/68 (30%), Positives = 34/68 (50%), Gaps = 8/68 (11%)
Query: 63 DVDVAKALKSLDFSNYKYIKRWWNHMRSFSDTEVSLFPDFRQPNFLKYNSKKILESQV-- 120
D++ + K LDFS+ H+R + SLF R + + S ++LESQ+
Sbjct: 580 DIEFSVTHKELDFSHLL------EHLRRILRGQASLFDWSRSTSLARTLSPRVLESQIDA 633
Query: 121 KEEVSKLL 128
K+E+ K L
Sbjct: 634 KKELEKCL 641
>At4g24170.1 68417.m03468 kinesin motor family protein contains Pfam
domain, PF00225: Kinesin motor domain
Length = 1004
Score = 28.7 bits (61), Expect = 9.2
Identities = 16/57 (28%), Positives = 30/57 (52%), Gaps = 1/57 (1%)
Query: 432 CEENKIPLAVVLGDSELKRGVVKIRNITTREEDEVPRNDLVQEIKKRIDNLNLNQLN 488
CE++ PL + L D E V K+R T R+ + + E +++I +LN+++
Sbjct: 142 CEDSSTPLRL-LDDPERGTVVEKLREETLRDRSHLEELLSICETQRKIGETSLNEIS 197
>At2g31970.1 68415.m03906 DNA repair-recombination protein (RAD50)
identical to DNA repair-recombination protein GI:7110148
from [Arabidopsis thaliana]
Length = 1316
Score = 28.7 bits (61), Expect = 9.2
Identities = 22/76 (28%), Positives = 39/76 (51%), Gaps = 3/76 (3%)
Query: 92 SDTEVSLFP-DFRQPNFLKYNSKKILESQVKEEVSKLLALKAQLVTEDAGPQKFTLKTPK 150
++ EV++ ++ N + K ES+ + SKL ALK + VT DA P+ L++ K
Sbjct: 605 AEKEVNMLQMKIQEVNNSLFKHNKDTESRKRYIESKLQALKQESVTIDAYPK--LLESAK 662
Query: 151 GTRDYNPQQMTIRNNV 166
RD ++ + N +
Sbjct: 663 DKRDDRKREYNMANGM 678
Database: arabidopsis
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.136 0.389
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,947,674
Number of Sequences: 28952
Number of extensions: 457823
Number of successful extensions: 1294
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 1278
Number of HSP's gapped (non-prelim): 20
length of query: 491
length of database: 12,070,560
effective HSP length: 84
effective length of query: 407
effective length of database: 9,638,592
effective search space: 3922906944
effective search space used: 3922906944
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
S2: 61 (28.7 bits)
- SilkBase 1999-2023 -