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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ps4M0068.Seq
         (868 letters)

Database: uniref50 
           1,657,284 sequences; 575,637,011 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_Q47336 Cluster: LacZ-alpha peptide; n=2; cellular organ...    82   2e-14
UniRef50_Q37953 Cluster: LacZ protein; n=1; Phage M13mp18|Rep: L...    82   2e-14
UniRef50_P00722 Cluster: Beta-galactosidase; n=35; root|Rep: Bet...    82   2e-14
UniRef50_Q8GEG0 Cluster: Putative uncharacterized protein; n=1; ...    81   3e-14
UniRef50_A7BPF2 Cluster: LacZ alpha peptide; n=1; Beggiatoa sp. ...    57   5e-07
UniRef50_Q669R9 Cluster: Beta-galactosidase; n=14; Yersinia|Rep:...    54   4e-06
UniRef50_UPI0000498F17 Cluster: beta-galactosidase; n=3; Eukaryo...    51   3e-05
UniRef50_P06219 Cluster: Beta-galactosidase; n=11; Gammaproteoba...    42   0.015
UniRef50_A0ZLG1 Cluster: Beta-D-galactosidase; n=1; Nodularia sp...    41   0.035
UniRef50_A7MN76 Cluster: Putative uncharacterized protein; n=1; ...    40   0.082
UniRef50_A6FJQ2 Cluster: 50S ribosomal protein L5; n=8; Bacteria...    40   0.082
UniRef50_Q4Z0C1 Cluster: Putative uncharacterized protein; n=3; ...    37   0.58 
UniRef50_UPI0000D9AEF2 Cluster: PREDICTED: similar to alpha 2 ty...    36   1.3  
UniRef50_UPI0000DD7CB1 Cluster: PREDICTED: hypothetical protein;...    35   2.3  
UniRef50_Q5XLD8 Cluster: Innexin 4; n=2; Bombyx|Rep: Innexin 4 -...    34   4.1  
UniRef50_A0D095 Cluster: Chromosome undetermined scaffold_33, wh...    34   4.1  
UniRef50_A2VBJ9 Cluster: Non-ribosomal peptide synthetase; n=1; ...    33   9.4  
UniRef50_A7S1U8 Cluster: Predicted protein; n=1; Nematostella ve...    33   9.4  

>UniRef50_Q47336 Cluster: LacZ-alpha peptide; n=2; cellular
           organisms|Rep: LacZ-alpha peptide - Escherichia coli
          Length = 90

 Score = 81.8 bits (193), Expect = 2e-14
 Identities = 36/37 (97%), Positives = 37/37 (100%)
 Frame = +3

Query: 255 ENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRT 365
           ENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLR+
Sbjct: 32  ENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS 68


>UniRef50_Q37953 Cluster: LacZ protein; n=1; Phage M13mp18|Rep: LacZ
           protein - Phage M13mp18
          Length = 102

 Score = 81.8 bits (193), Expect = 2e-14
 Identities = 36/37 (97%), Positives = 37/37 (100%)
 Frame = +3

Query: 255 ENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRT 365
           ENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLR+
Sbjct: 36  ENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS 72


>UniRef50_P00722 Cluster: Beta-galactosidase; n=35; root|Rep:
           Beta-galactosidase - Escherichia coli (strain K12)
          Length = 1024

 Score = 81.8 bits (193), Expect = 2e-14
 Identities = 36/37 (97%), Positives = 37/37 (100%)
 Frame = +3

Query: 255 ENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRT 365
           ENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLR+
Sbjct: 18  ENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS 54


>UniRef50_Q8GEG0 Cluster: Putative uncharacterized protein; n=1;
           Erwinia amylovora|Rep: Putative uncharacterized protein
           - Erwinia amylovora (Fire blight bacteria)
          Length = 123

 Score = 81.4 bits (192), Expect = 3e-14
 Identities = 36/36 (100%), Positives = 36/36 (100%)
 Frame = +3

Query: 255 ENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLR 362
           ENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLR
Sbjct: 78  ENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLR 113


>UniRef50_A7BPF2 Cluster: LacZ alpha peptide; n=1; Beggiatoa sp.
           SS|Rep: LacZ alpha peptide - Beggiatoa sp. SS
          Length = 73

 Score = 57.2 bits (132), Expect = 5e-07
 Identities = 25/27 (92%), Positives = 26/27 (96%)
 Frame = -2

Query: 705 FPGQALNRGLPLGFRFNALRHLDPKKL 625
           FP QALNRGLPLGFRF+ALRHLDPKKL
Sbjct: 46  FPRQALNRGLPLGFRFSALRHLDPKKL 72


>UniRef50_Q669R9 Cluster: Beta-galactosidase; n=14; Yersinia|Rep:
           Beta-galactosidase - Yersinia pseudotuberculosis
          Length = 1066

 Score = 54.4 bits (125), Expect = 4e-06
 Identities = 23/37 (62%), Positives = 28/37 (75%)
 Frame = +3

Query: 255 ENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRT 365
           ENP +TQ +RL AHPPF SWR+ E A+ DRPS Q +T
Sbjct: 25  ENPQITQYHRLEAHPPFHSWRDVESAQKDRPSPQQQT 61


>UniRef50_UPI0000498F17 Cluster: beta-galactosidase; n=3;
           Eukaryota|Rep: beta-galactosidase - Entamoeba
           histolytica HM-1:IMSS
          Length = 86

 Score = 51.2 bits (117), Expect = 3e-05
 Identities = 30/47 (63%), Positives = 34/47 (72%), Gaps = 7/47 (14%)
 Frame = +1

Query: 256 KTLALPNLIALQHIPLSPAGVIAK-----RPAP--IALPNSCAPEWR 375
           KTLALPNLIALQHIPLSPAGVI++     RP+    +L    APEWR
Sbjct: 16  KTLALPNLIALQHIPLSPAGVISEEARTDRPSQQLRSLKWRMAPEWR 62



 Score = 34.3 bits (75), Expect = 4.1
 Identities = 13/17 (76%), Positives = 13/17 (76%)
 Frame = +2

Query: 224 HWPSFYNVVTGKPWRYP 274
           HWPSFYNVVTGK    P
Sbjct: 5   HWPSFYNVVTGKTLALP 21


>UniRef50_P06219 Cluster: Beta-galactosidase; n=11;
           Gammaproteobacteria|Rep: Beta-galactosidase - Klebsiella
           pneumoniae
          Length = 1034

 Score = 42.3 bits (95), Expect = 0.015
 Identities = 19/35 (54%), Positives = 23/35 (65%)
 Frame = +3

Query: 258 NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLR 362
           N  +T LNRL AHP FASWR+   AR + PS + R
Sbjct: 25  NQTITHLNRLPAHPVFASWRDELAARDNLPSSRRR 59


>UniRef50_A0ZLG1 Cluster: Beta-D-galactosidase; n=1; Nodularia
           spumigena CCY 9414|Rep: Beta-D-galactosidase - Nodularia
           spumigena CCY 9414
          Length = 72

 Score = 41.1 bits (92), Expect = 0.035
 Identities = 17/18 (94%), Positives = 18/18 (100%)
 Frame = +3

Query: 312 WRNSEEARTDRPSQQLRT 365
           WRNSEEARTDRPSQQLR+
Sbjct: 47  WRNSEEARTDRPSQQLRS 64


>UniRef50_A7MN76 Cluster: Putative uncharacterized protein; n=1;
           Enterobacter sakazakii ATCC BAA-894|Rep: Putative
           uncharacterized protein - Enterobacter sakazakii ATCC
           BAA-894
          Length = 1043

 Score = 39.9 bits (89), Expect = 0.082
 Identities = 14/32 (43%), Positives = 21/32 (65%)
 Frame = +3

Query: 255 ENPGVTQLNRLAAHPPFASWRNSEEARTDRPS 350
           +NP +T +NRL +H P   WR+++ AR   PS
Sbjct: 28  QNPAITSVNRLPSHTPLHGWRDADRARRGEPS 59


>UniRef50_A6FJQ2 Cluster: 50S ribosomal protein L5; n=8;
           Bacteria|Rep: 50S ribosomal protein L5 - Moritella sp.
           PE36
          Length = 45

 Score = 39.9 bits (89), Expect = 0.082
 Identities = 22/29 (75%), Positives = 23/29 (79%)
 Frame = -3

Query: 383 FAIRHSGAQLLGRAIGAGLFAITPAGERG 297
           FAI+   AQLLGRAIGAGLFAITP  E G
Sbjct: 12  FAIQ--AAQLLGRAIGAGLFAITPEFELG 38


>UniRef50_Q4Z0C1 Cluster: Putative uncharacterized protein; n=3;
           Plasmodium (Vinckeia)|Rep: Putative uncharacterized
           protein - Plasmodium berghei
          Length = 275

 Score = 37.1 bits (82), Expect = 0.58
 Identities = 16/16 (100%), Positives = 16/16 (100%)
 Frame = +2

Query: 173 RGGARYPIRPIVSRIT 220
           RGGARYPIRPIVSRIT
Sbjct: 260 RGGARYPIRPIVSRIT 275


>UniRef50_UPI0000D9AEF2 Cluster: PREDICTED: similar to alpha 2 type
           VI collagen isoform 2C2 precursor; n=1; Macaca
           mulatta|Rep: PREDICTED: similar to alpha 2 type VI
           collagen isoform 2C2 precursor - Macaca mulatta
          Length = 235

 Score = 35.9 bits (79), Expect = 1.3
 Identities = 16/38 (42%), Positives = 22/38 (57%)
 Frame = +2

Query: 647 RKALNRNPKGSPRFRA*PGKSRRTWRXKGREEXRXGPG 760
           +KA    PK S   +  PG +R TWR +G++  R GPG
Sbjct: 38  QKASPGGPKDSRPHKEGPGTARLTWRTRGQQASRGGPG 75


>UniRef50_UPI0000DD7CB1 Cluster: PREDICTED: hypothetical protein;
           n=1; Homo sapiens|Rep: PREDICTED: hypothetical protein -
           Homo sapiens
          Length = 221

 Score = 35.1 bits (77), Expect = 2.3
 Identities = 27/83 (32%), Positives = 35/83 (42%)
 Frame = +2

Query: 569 GEKPSIQGDGPLREPSP*SSFLGSRCRKALNRNPKGSPRFRA*PGKSRRTWRXKGREEXR 748
           G +P++Q   P R+ +P     G     A    P+ SPR R  PG  R   R +GR   R
Sbjct: 5   GGRPALQKPSPARKRTPG----GCESSLAARARPR-SPRLRGRPGPGRGRGRSRGRSAAR 59

Query: 749 XGPGPXALGNFXRSPWXVNXPXP 817
             P   A G   R P   + P P
Sbjct: 60  DRP-DVAQGASARLPGPKSEPIP 81


>UniRef50_Q5XLD8 Cluster: Innexin 4; n=2; Bombyx|Rep: Innexin 4 -
           Bombyx mori (Silk moth)
          Length = 371

 Score = 34.3 bits (75), Expect = 4.1
 Identities = 20/51 (39%), Positives = 27/51 (52%)
 Frame = +2

Query: 65  F*LLVYWSIMLMPLVILAMY*RYLIKYLTEXXXXXTRGGARYPIRPIVSRI 217
           F +L +W I+L    ILA+  R+L+  L        RG  RY  R +VSRI
Sbjct: 279 FVILWFWLIILTAFSILAVVFRFLLLILYPLRTVMIRGQIRYVKRSVVSRI 329


>UniRef50_A0D095 Cluster: Chromosome undetermined scaffold_33, whole
           genome shotgun sequence; n=1; Paramecium
           tetraurelia|Rep: Chromosome undetermined scaffold_33,
           whole genome shotgun sequence - Paramecium tetraurelia
          Length = 1173

 Score = 34.3 bits (75), Expect = 4.1
 Identities = 15/44 (34%), Positives = 26/44 (59%)
 Frame = -2

Query: 495 VYSFDL*GILPISAYWLKNELI*QKFNANFNKILTLTICHSPFR 364
           +++F L G L    +WLKN+    KF++ F ++L L +  + FR
Sbjct: 665 IFNFSLQGALSYIDFWLKNQHFDDKFSSTFTQLLLLALGVTVFR 708


>UniRef50_A2VBJ9 Cluster: Non-ribosomal peptide synthetase; n=1;
           uncultured bacterium|Rep: Non-ribosomal peptide
           synthetase - uncultured bacterium
          Length = 338

 Score = 33.1 bits (72), Expect = 9.4
 Identities = 14/15 (93%), Positives = 14/15 (93%)
 Frame = -3

Query: 215 YDSL*GELGTGPPLE 171
           YDSL GELGTGPPLE
Sbjct: 278 YDSLYGELGTGPPLE 292


>UniRef50_A7S1U8 Cluster: Predicted protein; n=1; Nematostella
           vectensis|Rep: Predicted protein - Nematostella
           vectensis
          Length = 693

 Score = 33.1 bits (72), Expect = 9.4
 Identities = 22/50 (44%), Positives = 26/50 (52%)
 Frame = +2

Query: 554 TPTSKGEKPSIQGDGPLREPSP*SSFLGSRCRKALNRNPKGSPRFRA*PG 703
           TP S GEK SI   GP+  P P +   G+R R A  R P G+   R  PG
Sbjct: 377 TPGSPGEKGSIGDPGPVGPPGP-AGMRGTRGR-AGKRGPPGNDGPRGIPG 424


  Database: uniref50
    Posted date:  Oct 5, 2007 11:19 AM
  Number of letters in database: 575,637,011
  Number of sequences in database:  1,657,284
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 829,026,737
Number of Sequences: 1657284
Number of extensions: 17089942
Number of successful extensions: 34061
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 32830
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 34041
length of database: 575,637,011
effective HSP length: 100
effective length of database: 409,908,611
effective search space used: 77062818868
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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