SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= heS30019
         (573 letters)

Database: uniref50 
           1,657,284 sequences; 575,637,011 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_Q8GEG0 Cluster: Putative uncharacterized protein; n=1; ...    82   1e-14
UniRef50_Q47336 Cluster: LacZ-alpha peptide; n=2; cellular organ...    82   1e-14
UniRef50_Q37953 Cluster: LacZ protein; n=1; Phage M13mp18|Rep: L...    82   1e-14
UniRef50_P00722 Cluster: Beta-galactosidase; n=35; root|Rep: Bet...    82   1e-14
UniRef50_UPI0000498F17 Cluster: beta-galactosidase; n=3; Eukaryo...    81   2e-14
UniRef50_Q669R9 Cluster: Beta-galactosidase; n=14; Yersinia|Rep:...    56   4e-07
UniRef50_A7MN76 Cluster: Putative uncharacterized protein; n=1; ...    49   9e-05
UniRef50_P06219 Cluster: Beta-galactosidase; n=11; Gammaproteoba...    48   2e-04
UniRef50_P81650 Cluster: Beta-galactosidase; n=26; Gammaproteoba...    39   0.072
UniRef50_A0ZLG1 Cluster: Beta-D-galactosidase; n=1; Nodularia sp...    39   0.096
UniRef50_Q4Z0C1 Cluster: Putative uncharacterized protein; n=3; ...    37   0.29 
UniRef50_Q5DC94 Cluster: SJCHGC09076 protein; n=1; Schistosoma j...    37   0.39 
UniRef50_Q48727 Cluster: Beta-galactosidase; n=3; Lactococcus la...    36   0.68 
UniRef50_A0UVE2 Cluster: Glycoside hydrolase family 2, TIM barre...    36   0.89 
UniRef50_A0D095 Cluster: Chromosome undetermined scaffold_33, wh...    34   2.1  
UniRef50_A2VBJ9 Cluster: Non-ribosomal peptide synthetase; n=1; ...    34   2.7  
UniRef50_A0M224 Cluster: Beta-galactosidase; n=1; Gramella forse...    34   2.7  
UniRef50_A6FJQ2 Cluster: 50S ribosomal protein L5; n=8; Bacteria...    33   3.6  
UniRef50_Q15XN9 Cluster: Glycoside hydrolase family 2, TIM barre...    33   6.3  
UniRef50_Q2R308 Cluster: Putative uncharacterized protein; n=2; ...    33   6.3  
UniRef50_UPI0000F1EDC6 Cluster: PREDICTED: hypothetical protein;...    32   8.3  
UniRef50_UPI000038DE68 Cluster: COG0457: FOG: TPR repeat; n=1; N...    32   8.3  
UniRef50_Q4S5F6 Cluster: Chromosome 19 SCAF14731, whole genome s...    32   8.3  

>UniRef50_Q8GEG0 Cluster: Putative uncharacterized protein; n=1;
           Erwinia amylovora|Rep: Putative uncharacterized protein
           - Erwinia amylovora (Fire blight bacteria)
          Length = 123

 Score = 81.8 bits (193), Expect = 1e-14
 Identities = 36/36 (100%), Positives = 36/36 (100%)
 Frame = +2

Query: 146 LAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEA 253
           LAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEA
Sbjct: 68  LAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEA 103



 Score = 35.5 bits (78), Expect = 0.89
 Identities = 14/16 (87%), Positives = 15/16 (93%)
 Frame = +1

Query: 256 TDRPSQQLRSLNGEWQ 303
           TDRPSQQLR LNGEW+
Sbjct: 105 TDRPSQQLRXLNGEWR 120


>UniRef50_Q47336 Cluster: LacZ-alpha peptide; n=2; cellular
           organisms|Rep: LacZ-alpha peptide - Escherichia coli
          Length = 90

 Score = 81.8 bits (193), Expect = 1e-14
 Identities = 36/36 (100%), Positives = 36/36 (100%)
 Frame = +2

Query: 146 LAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEA 253
           LAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEA
Sbjct: 22  LAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEA 57


>UniRef50_Q37953 Cluster: LacZ protein; n=1; Phage M13mp18|Rep: LacZ
           protein - Phage M13mp18
          Length = 102

 Score = 81.8 bits (193), Expect = 1e-14
 Identities = 36/36 (100%), Positives = 36/36 (100%)
 Frame = +2

Query: 146 LAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEA 253
           LAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEA
Sbjct: 26  LAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEA 61



 Score = 37.5 bits (83), Expect = 0.22
 Identities = 15/16 (93%), Positives = 16/16 (100%)
 Frame = +1

Query: 256 TDRPSQQLRSLNGEWQ 303
           TDRPSQQLRSLNGEW+
Sbjct: 63  TDRPSQQLRSLNGEWR 78


>UniRef50_P00722 Cluster: Beta-galactosidase; n=35; root|Rep:
           Beta-galactosidase - Escherichia coli (strain K12)
          Length = 1024

 Score = 81.8 bits (193), Expect = 1e-14
 Identities = 36/36 (100%), Positives = 36/36 (100%)
 Frame = +2

Query: 146 LAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEA 253
           LAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEA
Sbjct: 8   LAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEA 43



 Score = 37.5 bits (83), Expect = 0.22
 Identities = 15/16 (93%), Positives = 16/16 (100%)
 Frame = +1

Query: 256 TDRPSQQLRSLNGEWQ 303
           TDRPSQQLRSLNGEW+
Sbjct: 45  TDRPSQQLRSLNGEWR 60


>UniRef50_UPI0000498F17 Cluster: beta-galactosidase; n=3;
           Eukaryota|Rep: beta-galactosidase - Entamoeba
           histolytica HM-1:IMSS
          Length = 86

 Score = 81.0 bits (191), Expect = 2e-14
 Identities = 38/54 (70%), Positives = 42/54 (77%), Gaps = 1/54 (1%)
 Frame = +3

Query: 144 HWPSFYNVVTGKTLALPNLIALQHIPLSPAGVIAKRPHRS-PFPTVAQPEWRMA 302
           HWPSFYNVVTGKTLALPNLIALQHIPLSPAGVI++      P   +   +WRMA
Sbjct: 5   HWPSFYNVVTGKTLALPNLIALQHIPLSPAGVISEEARTDRPSQQLRSLKWRMA 58


>UniRef50_Q669R9 Cluster: Beta-galactosidase; n=14; Yersinia|Rep:
           Beta-galactosidase - Yersinia pseudotuberculosis
          Length = 1066

 Score = 56.4 bits (130), Expect = 4e-07
 Identities = 23/36 (63%), Positives = 27/36 (75%)
 Frame = +2

Query: 146 LAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEA 253
           L  +L RRDWENP +TQ +RL AHPPF SWR+ E A
Sbjct: 15  LPQILSRRDWENPQITQYHRLEAHPPFHSWRDVESA 50


>UniRef50_A7MN76 Cluster: Putative uncharacterized protein; n=1;
           Enterobacter sakazakii ATCC BAA-894|Rep: Putative
           uncharacterized protein - Enterobacter sakazakii ATCC
           BAA-894
          Length = 1043

 Score = 48.8 bits (111), Expect = 9e-05
 Identities = 17/36 (47%), Positives = 25/36 (69%)
 Frame = +2

Query: 146 LAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEA 253
           LA +L R DW+NP +T +NRL +H P   WR+++ A
Sbjct: 18  LATILARNDWQNPAITSVNRLPSHTPLHGWRDADRA 53


>UniRef50_P06219 Cluster: Beta-galactosidase; n=11;
           Gammaproteobacteria|Rep: Beta-galactosidase - Klebsiella
           pneumoniae
          Length = 1034

 Score = 48.0 bits (109), Expect = 2e-04
 Identities = 24/41 (58%), Positives = 27/41 (65%)
 Frame = +2

Query: 155 VLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAPPIALPNS 277
           VL R DW N  +T LNRL AHP FASWR+ E A    LP+S
Sbjct: 17  VLAREDWHNQTITHLNRLPAHPVFASWRD-ELAARDNLPSS 56


>UniRef50_P81650 Cluster: Beta-galactosidase; n=26;
           Gammaproteobacteria|Rep: Beta-galactosidase -
           Pseudoalteromonas haloplanktis (Alteromonas
           haloplanktis)
          Length = 1039

 Score = 39.1 bits (87), Expect = 0.072
 Identities = 14/33 (42%), Positives = 22/33 (66%)
 Frame = +2

Query: 155 VLQRRDWENPGVTQLNRLAAHPPFASWRNSEEA 253
           ++ RRDWENP   Q+N++ AH P   ++  E+A
Sbjct: 7   IINRRDWENPITVQVNQVKAHSPLNGFKTIEDA 39


>UniRef50_A0ZLG1 Cluster: Beta-D-galactosidase; n=1; Nodularia
           spumigena CCY 9414|Rep: Beta-D-galactosidase - Nodularia
           spumigena CCY 9414
          Length = 72

 Score = 38.7 bits (86), Expect = 0.096
 Identities = 15/18 (83%), Positives = 18/18 (100%)
 Frame = +1

Query: 256 TDRPSQQLRSLNGEWQIV 309
           TDRPSQQLRSLNGEW+++
Sbjct: 55  TDRPSQQLRSLNGEWRLM 72


>UniRef50_Q4Z0C1 Cluster: Putative uncharacterized protein; n=3;
           Plasmodium (Vinckeia)|Rep: Putative uncharacterized
           protein - Plasmodium berghei
          Length = 275

 Score = 37.1 bits (82), Expect = 0.29
 Identities = 16/16 (100%), Positives = 16/16 (100%)
 Frame = +3

Query: 93  RGGARYPIRPIVSRIT 140
           RGGARYPIRPIVSRIT
Sbjct: 260 RGGARYPIRPIVSRIT 275


>UniRef50_Q5DC94 Cluster: SJCHGC09076 protein; n=1; Schistosoma
           japonicum|Rep: SJCHGC09076 protein - Schistosoma
           japonicum (Blood fluke)
          Length = 109

 Score = 36.7 bits (81), Expect = 0.39
 Identities = 17/37 (45%), Positives = 23/37 (62%)
 Frame = +2

Query: 149 AVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAPP 259
           A  L+RR+ +NPG  QLN L A P F     +++APP
Sbjct: 57  AAFLKRREGKNPGCPQLNPLEALPLFPGGEKTKKAPP 93


>UniRef50_Q48727 Cluster: Beta-galactosidase; n=3; Lactococcus
           lactis|Rep: Beta-galactosidase - Lactococcus lactis
           subsp. lactis (Streptococcus lactis)
          Length = 998

 Score = 35.9 bits (79), Expect = 0.68
 Identities = 14/23 (60%), Positives = 17/23 (73%)
 Frame = +2

Query: 155 VLQRRDWENPGVTQLNRLAAHPP 223
           VL+R+DWENP V+  NRL  H P
Sbjct: 9   VLERKDWENPVVSNWNRLPMHTP 31


>UniRef50_A0UVE2 Cluster: Glycoside hydrolase family 2, TIM barrel;
           n=1; Clostridium cellulolyticum H10|Rep: Glycoside
           hydrolase family 2, TIM barrel - Clostridium
           cellulolyticum H10
          Length = 1033

 Score = 35.5 bits (78), Expect = 0.89
 Identities = 12/29 (41%), Positives = 20/29 (68%)
 Frame = +2

Query: 167 RDWENPGVTQLNRLAAHPPFASWRNSEEA 253
           R+WEN  +TQ+NR   H P+ ++ + E+A
Sbjct: 3   REWENQYITQINRYPMHSPYGAYESVEQA 31


>UniRef50_A0D095 Cluster: Chromosome undetermined scaffold_33, whole
           genome shotgun sequence; n=1; Paramecium
           tetraurelia|Rep: Chromosome undetermined scaffold_33,
           whole genome shotgun sequence - Paramecium tetraurelia
          Length = 1173

 Score = 34.3 bits (75), Expect = 2.1
 Identities = 15/44 (34%), Positives = 26/44 (59%)
 Frame = -2

Query: 416 VYSFDL*GILPISAYWLKNELI*QKFNANFNKILTLTICHSPFR 285
           +++F L G L    +WLKN+    KF++ F ++L L +  + FR
Sbjct: 665 IFNFSLQGALSYIDFWLKNQHFDDKFSSTFTQLLLLALGVTVFR 708


>UniRef50_A2VBJ9 Cluster: Non-ribosomal peptide synthetase; n=1;
           uncultured bacterium|Rep: Non-ribosomal peptide
           synthetase - uncultured bacterium
          Length = 338

 Score = 33.9 bits (74), Expect = 2.7
 Identities = 18/35 (51%), Positives = 18/35 (51%)
 Frame = -1

Query: 195 WVTPGFSQSRRCKTTASEL*YDSL*GELGTGPPLE 91
           W   GF     C        YDSL GELGTGPPLE
Sbjct: 260 WSKTGFRPF--CLEAGRRAYYDSLYGELGTGPPLE 292


>UniRef50_A0M224 Cluster: Beta-galactosidase; n=1; Gramella forsetii
           KT0803|Rep: Beta-galactosidase - Gramella forsetii
           (strain KT0803)
          Length = 1049

 Score = 33.9 bits (74), Expect = 2.7
 Identities = 17/44 (38%), Positives = 22/44 (50%)
 Frame = +2

Query: 170 DWENPGVTQLNRLAAHPPFASWRNSEEAPPIALPNSCAA*MANG 301
           DWENP VT +N+L A     S+ N + A  +   NS      NG
Sbjct: 26  DWENPAVTGINKLPARATMYSFSNKQAAINLNKENSDRVKSLNG 69


>UniRef50_A6FJQ2 Cluster: 50S ribosomal protein L5; n=8;
           Bacteria|Rep: 50S ribosomal protein L5 - Moritella sp.
           PE36
          Length = 45

 Score = 33.5 bits (73), Expect = 3.6
 Identities = 15/18 (83%), Positives = 16/18 (88%)
 Frame = -1

Query: 309 YNLPFAIQAAQLLGRAIG 256
           +  PFAIQAAQLLGRAIG
Sbjct: 8   HQAPFAIQAAQLLGRAIG 25


>UniRef50_Q15XN9 Cluster: Glycoside hydrolase family 2, TIM barrel
           precursor; n=1; Pseudoalteromonas atlantica T6c|Rep:
           Glycoside hydrolase family 2, TIM barrel precursor -
           Pseudoalteromonas atlantica (strain T6c / BAA-1087)
          Length = 1079

 Score = 32.7 bits (71), Expect = 6.3
 Identities = 14/30 (46%), Positives = 18/30 (60%)
 Frame = +2

Query: 164 RRDWENPGVTQLNRLAAHPPFASWRNSEEA 253
           + DWENP V Q+NRL A     S+   E+A
Sbjct: 31  KNDWENPDVIQINRLPARATSYSFDTPEQA 60


>UniRef50_Q2R308 Cluster: Putative uncharacterized protein; n=2;
           Oryza sativa|Rep: Putative uncharacterized protein -
           Oryza sativa subsp. japonica (Rice)
          Length = 103

 Score = 32.7 bits (71), Expect = 6.3
 Identities = 11/18 (61%), Positives = 13/18 (72%)
 Frame = -2

Query: 293 PFRLRNCWEGRSVGPLRY 240
           PFR R CW  R+VGP R+
Sbjct: 35  PFRSRTCWHSRAVGPTRF 52


>UniRef50_UPI0000F1EDC6 Cluster: PREDICTED: hypothetical protein;
           n=1; Danio rerio|Rep: PREDICTED: hypothetical protein -
           Danio rerio
          Length = 195

 Score = 32.3 bits (70), Expect = 8.3
 Identities = 13/13 (100%), Positives = 13/13 (100%)
 Frame = +2

Query: 146 LAVVLQRRDWENP 184
           LAVVLQRRDWENP
Sbjct: 179 LAVVLQRRDWENP 191


>UniRef50_UPI000038DE68 Cluster: COG0457: FOG: TPR repeat; n=1;
           Nostoc punctiforme PCC 73102|Rep: COG0457: FOG: TPR
           repeat - Nostoc punctiforme PCC 73102
          Length = 532

 Score = 32.3 bits (70), Expect = 8.3
 Identities = 14/37 (37%), Positives = 23/37 (62%), Gaps = 1/37 (2%)
 Frame = +2

Query: 146 LAVVLQRRDWENPGVTQLNRLAAH-PPFASWRNSEEA 253
           + V+L+  DWE P + QL+ L ++  P  SW + +EA
Sbjct: 96  IPVLLRYADWETPPIDQLSPLPSNRKPIKSWNDRDEA 132


>UniRef50_Q4S5F6 Cluster: Chromosome 19 SCAF14731, whole genome
           shotgun sequence; n=2; Clupeocephala|Rep: Chromosome 19
           SCAF14731, whole genome shotgun sequence - Tetraodon
           nigroviridis (Green puffer)
          Length = 842

 Score = 32.3 bits (70), Expect = 8.3
 Identities = 21/58 (36%), Positives = 29/58 (50%), Gaps = 3/58 (5%)
 Frame = +3

Query: 138 TIHWPSFYNVVTGKTLALPNLIALQHIPLSPAG---VIAKRPHRSPFPTVAQPEWRMA 302
           ++H  S Y   +  T AL  L +  H+ LSP+    V  K+   SPFP V QP   +A
Sbjct: 755 SLHPSSLYKTPSPSTPALSPLSSSSHLSLSPSSPGDVPPKQQVFSPFPCVKQPRKSVA 812


  Database: uniref50
    Posted date:  Oct 5, 2007 11:19 AM
  Number of letters in database: 575,637,011
  Number of sequences in database:  1,657,284
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 555,114,538
Number of Sequences: 1657284
Number of extensions: 11578093
Number of successful extensions: 28601
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 27840
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 28597
length of database: 575,637,011
effective HSP length: 96
effective length of database: 416,537,747
effective search space used: 39154548218
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -