SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= e96h0033
         (690 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_46721| Best HMM Match : Tetraspannin (HMM E-Value=4e-35)            53   2e-07
SB_1751| Best HMM Match : No HMM Matches (HMM E-Value=.)               51   8e-07
SB_15887| Best HMM Match : No HMM Matches (HMM E-Value=.)              50   2e-06
SB_23554| Best HMM Match : No HMM Matches (HMM E-Value=.)              50   2e-06
SB_26670| Best HMM Match : Tetraspannin (HMM E-Value=1.09301e-43)      40   0.002
SB_41644| Best HMM Match : Tetraspannin (HMM E-Value=0)                40   0.003
SB_23671| Best HMM Match : Tetraspannin (HMM E-Value=6.6e-38)          39   0.003
SB_31429| Best HMM Match : Tetraspannin (HMM E-Value=5.9e-35)          37   0.013
SB_22249| Best HMM Match : No HMM Matches (HMM E-Value=.)              35   0.071
SB_31428| Best HMM Match : Tetraspannin (HMM E-Value=0)                34   0.12 
SB_31427| Best HMM Match : Tetraspannin (HMM E-Value=0)                34   0.12 
SB_22250| Best HMM Match : No HMM Matches (HMM E-Value=.)              33   0.17 
SB_2984| Best HMM Match : Tetraspannin (HMM E-Value=9e-06)             32   0.38 
SB_47590| Best HMM Match : TSP_3 (HMM E-Value=7.6e-09)                 30   1.5  
SB_32096| Best HMM Match : Tetraspannin (HMM E-Value=1.1e-13)          30   2.0  
SB_58401| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   2.7  
SB_57558| Best HMM Match : No HMM Matches (HMM E-Value=.)              28   6.2  
SB_38027| Best HMM Match : No HMM Matches (HMM E-Value=.)              28   6.2  
SB_15543| Best HMM Match : DUF1311 (HMM E-Value=6.2)                   28   8.2  

>SB_46721| Best HMM Match : Tetraspannin (HMM E-Value=4e-35)
          Length = 642

 Score = 53.2 bits (122), Expect = 2e-07
 Identities = 21/56 (37%), Positives = 34/56 (60%)
 Frame = +2

Query: 314 CCGAVKENHCMIITFSVFLLIIFVAELAVGIAGYMKHTDLEDSVMRNLNASITQYP 481
           CCGA KEN CM+I F + LLI+   E+A  + GY+    ++  +  ++  +I +YP
Sbjct: 425 CCGAWKENKCMLILFFILLLIVLGLEIAAAVLGYINKDKIQSDLTDDIIRNIKEYP 480



 Score = 32.7 bits (71), Expect = 0.29
 Identities = 16/58 (27%), Positives = 24/58 (41%), Gaps = 4/58 (6%)
 Frame = +1

Query: 514 IQTDLQCCGINSPADWADHG----LPIPSTCCSAQEINDGVVAACTENSTNFHSKGWS 675
           +Q D +CCG   P+DW  +G       P +CC     ND  +     +     +  WS
Sbjct: 490 MQMDFECCGAKGPSDWTFYGQMKYKAGPGSCCGKPN-NDACLPGTRNSKLPRSTHTWS 546


>SB_1751| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 341

 Score = 51.2 bits (117), Expect = 8e-07
 Identities = 21/56 (37%), Positives = 33/56 (58%)
 Frame = +2

Query: 314 CCGAVKENHCMIITFSVFLLIIFVAELAVGIAGYMKHTDLEDSVMRNLNASITQYP 481
           CCGA KEN CM+I F  FLL++ + E+  G   Y     +E+ + +++  +I  YP
Sbjct: 72  CCGAWKENKCMLICFFAFLLLLLILEIVGGALAYNNKDKIENRLDKDILKAIENYP 127



 Score = 38.3 bits (85), Expect = 0.006
 Identities = 19/50 (38%), Positives = 27/50 (54%)
 Frame = +3

Query: 99  MALEGGMSCVKYLMFCFNLLFAITGLIILIVGIRAEINSYPYMNFTDENF 248
           MAL+G  + VK L+  FN +F + GLI+  VGI A      Y+     N+
Sbjct: 1   MALQGSSNIVKILVIIFNFIFFLFGLILFGVGIWASTKLGAYVEIASVNY 50



 Score = 31.1 bits (67), Expect = 0.88
 Identities = 12/42 (28%), Positives = 22/42 (52%), Gaps = 5/42 (11%)
 Frame = +1

Query: 514 IQTDLQCCGINSPADWAD-----HGLPIPSTCCSAQEINDGV 624
           +QT  +CCG ++  DW       +   +P +CC +++   GV
Sbjct: 137 MQTKFKCCGADNYTDWQSNIKMKNSSSVPDSCCKSEKAGCGV 178



 Score = 29.5 bits (63), Expect = 2.7
 Identities = 15/42 (35%), Positives = 26/42 (61%), Gaps = 5/42 (11%)
 Frame = +1

Query: 514 IQTDLQCCGINSPADW--ADH-GLP--IPSTCCSAQEINDGV 624
           +Q  ++CCGI +  DW  +D+ GL   +P +CC+  E + G+
Sbjct: 245 MQKTVKCCGITTYEDWKSSDNLGLTDNVPDSCCTTVEKDCGM 286


>SB_15887| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 333

 Score = 49.6 bits (113), Expect = 2e-06
 Identities = 23/70 (32%), Positives = 42/70 (60%)
 Frame = +2

Query: 314 CCGAVKENHCMIITFSVFLLIIFVAELAVGIAGYMKHTDLEDSVMRNLNASITQYPVDKN 493
           C GA++EN C++  FSV L IIF  +LA+G+  ++    +E  ++  L  ++T+Y  + +
Sbjct: 65  CIGALRENICLLKFFSVVLAIIFFLQLALGVFVFVFQDKVEAVIVEKLQTAVTKYRDNAD 124

Query: 494 VQKTIISYKQ 523
           +Q  I   +Q
Sbjct: 125 LQNLIDGVQQ 134


>SB_23554| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 514

 Score = 49.6 bits (113), Expect = 2e-06
 Identities = 19/55 (34%), Positives = 32/55 (58%)
 Frame = +2

Query: 314 CCGAVKENHCMIITFSVFLLIIFVAELAVGIAGYMKHTDLEDSVMRNLNASITQY 478
           CCGA+KE+ C++ TF   L II   E+     GY+    +E+ + +++N +I  Y
Sbjct: 344 CCGAIKEHRCLLATFFALLFIILAVEITGTALGYVYRNKVEEQLGKDMNDTIVSY 398



 Score = 31.9 bits (69), Expect = 0.50
 Identities = 17/59 (28%), Positives = 26/59 (44%), Gaps = 7/59 (11%)
 Frame = +1

Query: 469 YSISGRQERPEDYYIIQTDLQCCGINSPADW-------ADHGLPIPSTCCSAQEINDGV 624
           Y   G++   + Y  IQ   +CCGIN   DW        +H + +P +CC       G+
Sbjct: 398 YGEKGKEGITDAYDAIQKKEKCCGINGFKDWQRSYYTKGNHSI-VPDSCCKTVSKGCGI 455


>SB_26670| Best HMM Match : Tetraspannin (HMM E-Value=1.09301e-43)
          Length = 211

 Score = 39.9 bits (89), Expect = 0.002
 Identities = 17/34 (50%), Positives = 21/34 (61%)
 Frame = +2

Query: 314 CCGAVKENHCMIITFSVFLLIIFVAELAVGIAGY 415
           C GA KEN CM+ TF V LLI+   E+A  +  Y
Sbjct: 74  CLGAWKENRCMLGTFFVLLLILLTLEIAAAVLAY 107



 Score = 28.7 bits (61), Expect = 4.7
 Identities = 13/48 (27%), Positives = 22/48 (45%), Gaps = 5/48 (10%)
 Frame = +1

Query: 469 YSISGRQERPEDYYIIQTDLQCCGINSPADWA-----DHGLPIPSTCC 597
           Y  SG+    + +  +Q   +CCG+N   DW      +    +P +CC
Sbjct: 138 YGSSGQDGVTKAFDALQEKQKCCGVNGYLDWKASKKYNGTSTVPDSCC 185



 Score = 27.9 bits (59), Expect = 8.2
 Identities = 13/33 (39%), Positives = 20/33 (60%)
 Frame = +3

Query: 111 GGMSCVKYLMFCFNLLFAITGLIILIVGIRAEI 209
           G    +K L+  FN+LF I G+ ++ VG  AE+
Sbjct: 7   GAGRILKILVVFFNVLFFIFGIALIAVGAWAEV 39


>SB_41644| Best HMM Match : Tetraspannin (HMM E-Value=0)
          Length = 252

 Score = 39.5 bits (88), Expect = 0.003
 Identities = 15/55 (27%), Positives = 32/55 (58%)
 Frame = +2

Query: 314 CCGAVKENHCMIITFSVFLLIIFVAELAVGIAGYMKHTDLEDSVMRNLNASITQY 478
           CCGA+KE+ CM+ T+   +  IF  E+   +  Y+    +E ++  ++ +++ +Y
Sbjct: 77  CCGALKESQCMLGTYFGLVTAIFCLEVTAIVLAYVYRERIEKNLHGDIRSTMNEY 131



 Score = 37.9 bits (84), Expect = 0.008
 Identities = 13/33 (39%), Positives = 22/33 (66%)
 Frame = +3

Query: 99  MALEGGMSCVKYLMFCFNLLFAITGLIILIVGI 197
           M+ + G  C+K+++F FNLLF + G  I  +G+
Sbjct: 1   MSTDWGFKCIKFMVFFFNLLFLLAGCAIFAIGV 33


>SB_23671| Best HMM Match : Tetraspannin (HMM E-Value=6.6e-38)
          Length = 919

 Score = 39.1 bits (87), Expect = 0.003
 Identities = 14/65 (21%), Positives = 34/65 (52%)
 Frame = +2

Query: 314 CCGAVKENHCMIITFSVFLLIIFVAELAVGIAGYMKHTDLEDSVMRNLNASITQYPVDKN 493
           C   +KEN  +++++ + L++IF+ E+A G+   +  +++   +   +   + +Y     
Sbjct: 78  CVAVIKENRFILVSYLIMLVLIFILEIATGVVAVIYRSEISYELEVGIRGKMNRYGFTDA 137

Query: 494 VQKTI 508
           V K I
Sbjct: 138 VTKAI 142


>SB_31429| Best HMM Match : Tetraspannin (HMM E-Value=5.9e-35)
          Length = 286

 Score = 37.1 bits (82), Expect = 0.013
 Identities = 19/71 (26%), Positives = 35/71 (49%)
 Frame = +2

Query: 314 CCGAVKENHCMIITFSVFLLIIFVAELAVGIAGYMKHTDLEDSVMRNLNASITQYPVDKN 493
           C GA+KEN   + ++ +F+    +  +A G+  +++   L D   R L   +  Y  D +
Sbjct: 91  CVGALKENTLFLASYGIFIFCCLIPIIAAGVLAFLQREKLVDPKAR-LEDGLKSYQQDSS 149

Query: 494 VQKTIISYKQT 526
            Q+  I + QT
Sbjct: 150 KQE-FIDFIQT 159



 Score = 29.1 bits (62), Expect = 3.6
 Identities = 13/32 (40%), Positives = 17/32 (53%), Gaps = 5/32 (15%)
 Frame = +1

Query: 514 IQTDLQCCGINSPADWADH-----GLPIPSTC 594
           IQT  +CCGI S  DW  +       P+P+ C
Sbjct: 157 IQTRFKCCGIESYKDWQANRYFSCSSPVPTAC 188


>SB_22249| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 296

 Score = 34.7 bits (76), Expect = 0.071
 Identities = 17/45 (37%), Positives = 25/45 (55%)
 Frame = +1

Query: 514 IQTDLQCCGINSPADWADHGLPIPSTCCSAQEINDGVVAACTENS 648
           +QT+L+CCG+NS  DW  +   I   C S    + GV  +C + S
Sbjct: 165 LQTNLKCCGVNSYTDWQTN---IYFNCSSPGSESCGVPFSCCKES 206



 Score = 33.5 bits (73), Expect = 0.17
 Identities = 10/25 (40%), Positives = 21/25 (84%)
 Frame = +3

Query: 120 SCVKYLMFCFNLLFAITGLIILIVG 194
           +C+KY++F  N++F + G++IL++G
Sbjct: 37  TCIKYILFFVNVVFWVFGVLILVIG 61



 Score = 28.3 bits (60), Expect = 6.2
 Identities = 19/72 (26%), Positives = 34/72 (47%), Gaps = 1/72 (1%)
 Frame = +2

Query: 314 CCGAVKENHCMIITFSVFLLIIFVAELAVGIAGYMKHTDLEDSVMRNLNASITQY-PVDK 490
           C GA++EN  ++  +   + +  +  ++ GIA YM     +D V+  L   +  Y   DK
Sbjct: 100 CIGALRENTILLAIYGGAVTLCLIFLVSAGIAVYMH----QDKVVEKLKDDLKHYRDPDK 155

Query: 491 NVQKTIISYKQT 526
              + +I   QT
Sbjct: 156 ENFQFLIDRLQT 167


>SB_31428| Best HMM Match : Tetraspannin (HMM E-Value=0)
          Length = 280

 Score = 33.9 bits (74), Expect = 0.12
 Identities = 16/71 (22%), Positives = 34/71 (47%)
 Frame = +2

Query: 314 CCGAVKENHCMIITFSVFLLIIFVAELAVGIAGYMKHTDLEDSVMRNLNASITQYPVDKN 493
           C GA++EN C +  +   + +  +  +A G   Y +  D   ++   L   + +Y +D  
Sbjct: 96  CVGALRENTCFLTVYGTSVFLCLILLIACGAVAYTQ-KDKVQALKERLKQDLKKY-LDDP 153

Query: 494 VQKTIISYKQT 526
           + + +I + QT
Sbjct: 154 LNQDLIDFMQT 164



 Score = 32.7 bits (71), Expect = 0.29
 Identities = 11/25 (44%), Positives = 18/25 (72%)
 Frame = +3

Query: 123 CVKYLMFCFNLLFAITGLIILIVGI 197
           C KY +F  N++F I GL+++ VG+
Sbjct: 27  CTKYTLFFINVIFFILGLLVMTVGV 51



 Score = 31.5 bits (68), Expect = 0.67
 Identities = 16/49 (32%), Positives = 25/49 (51%)
 Frame = +1

Query: 514 IQTDLQCCGINSPADWADHGLPIPSTCCSAQEINDGVVAACTENSTNFH 660
           +QT L+CCG+ S  DW  +       C S   ++ GV  +C + S  F+
Sbjct: 162 MQTLLKCCGVESYKDWQANPY---FNCSSPGRMSCGVPFSCCKESIQFN 207


>SB_31427| Best HMM Match : Tetraspannin (HMM E-Value=0)
          Length = 299

 Score = 33.9 bits (74), Expect = 0.12
 Identities = 17/45 (37%), Positives = 25/45 (55%)
 Frame = +1

Query: 514 IQTDLQCCGINSPADWADHGLPIPSTCCSAQEINDGVVAACTENS 648
           +QT+L+CCGINS  DW  +   +   C S    + GV  +C + S
Sbjct: 165 LQTNLKCCGINSYNDWQTN---VYFNCSSPGSESCGVPFSCCKES 206



 Score = 33.5 bits (73), Expect = 0.17
 Identities = 10/25 (40%), Positives = 21/25 (84%)
 Frame = +3

Query: 120 SCVKYLMFCFNLLFAITGLIILIVG 194
           +C+KY++F  N++F + G++IL++G
Sbjct: 37  TCIKYILFFVNVVFWVFGVLILVIG 61


>SB_22250| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 313

 Score = 33.5 bits (73), Expect = 0.17
 Identities = 13/25 (52%), Positives = 18/25 (72%)
 Frame = +3

Query: 123 CVKYLMFCFNLLFAITGLIILIVGI 197
           CVKY +F  N++F + G +IL VGI
Sbjct: 59  CVKYSLFTINVIFWLAGAVILAVGI 83



 Score = 31.5 bits (68), Expect = 0.67
 Identities = 16/55 (29%), Positives = 24/55 (43%)
 Frame = +2

Query: 314 CCGAVKENHCMIITFSVFLLIIFVAELAVGIAGYMKHTDLEDSVMRNLNASITQY 478
           C GA++EN C++  +     II    +  G  G+     LE  V   L  +I  Y
Sbjct: 122 CIGALRENRCLLTAYIWMCGIILAGMIVCGGLGFYYKDVLETKVTAQLKDAIVLY 176



 Score = 29.5 bits (63), Expect = 2.7
 Identities = 9/16 (56%), Positives = 12/16 (75%)
 Frame = +1

Query: 514 IQTDLQCCGINSPADW 561
           +QT+LQCCG+    DW
Sbjct: 191 VQTELQCCGVQGLNDW 206


>SB_2984| Best HMM Match : Tetraspannin (HMM E-Value=9e-06)
          Length = 187

 Score = 32.3 bits (70), Expect = 0.38
 Identities = 12/44 (27%), Positives = 25/44 (56%)
 Frame = +2

Query: 350 ITFSVFLLIIFVAELAVGIAGYMKHTDLEDSVMRNLNASITQYP 481
           + F V L ++F+ EL   I GY+  ++++  +   L+ ++  YP
Sbjct: 130 LQFGVILGLLFLVELGTAITGYIFRSEIKTGLSDGLDTALKDYP 173


>SB_47590| Best HMM Match : TSP_3 (HMM E-Value=7.6e-09)
          Length = 669

 Score = 30.3 bits (65), Expect = 1.5
 Identities = 11/22 (50%), Positives = 16/22 (72%)
 Frame = +3

Query: 114 GMSCVKYLMFCFNLLFAITGLI 179
           GM C+KYL+F FN +F  + +I
Sbjct: 76  GMQCMKYLLFAFNFVFWCSKII 97



 Score = 29.1 bits (62), Expect = 3.6
 Identities = 10/16 (62%), Positives = 12/16 (75%)
 Frame = +2

Query: 314 CCGAVKENHCMIITFS 361
           CCGAVKEN C ++  S
Sbjct: 217 CCGAVKENKCFLLLVS 232


>SB_32096| Best HMM Match : Tetraspannin (HMM E-Value=1.1e-13)
          Length = 174

 Score = 29.9 bits (64), Expect = 2.0
 Identities = 14/37 (37%), Positives = 20/37 (54%), Gaps = 6/37 (16%)
 Frame = +1

Query: 514 IQTDLQCCGINS-PADWADH-----GLPIPSTCCSAQ 606
           IQ  LQCCG +S P DW ++        +P +CC  +
Sbjct: 90  IQERLQCCGADSGPNDWMNNKHFLQSASVPDSCCKQE 126


>SB_58401| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 426

 Score = 29.5 bits (63), Expect = 2.7
 Identities = 10/14 (71%), Positives = 12/14 (85%)
 Frame = +2

Query: 314 CCGAVKENHCMIIT 355
           CCGAVKE+ CM+ T
Sbjct: 332 CCGAVKESRCMLAT 345


>SB_57558| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 733

 Score = 28.3 bits (60), Expect = 6.2
 Identities = 12/28 (42%), Positives = 18/28 (64%)
 Frame = -3

Query: 211 FISALMPTIRMINPVMANRRLKQNMRYF 128
           FI+ALM  I +I  +M  + L++ M YF
Sbjct: 39  FITALMGNIILITVIMKTKSLRRTMNYF 66


>SB_38027| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 316

 Score = 28.3 bits (60), Expect = 6.2
 Identities = 10/30 (33%), Positives = 19/30 (63%)
 Frame = +2

Query: 317 CGAVKENHCMIITFSVFLLIIFVAELAVGI 406
           C  +  N C++ T+  F+LI+ VAE+ + +
Sbjct: 38  CALLYMNPCLLKTYFSFMLILVVAEVTIAV 67


>SB_15543| Best HMM Match : DUF1311 (HMM E-Value=6.2)
          Length = 163

 Score = 27.9 bits (59), Expect = 8.2
 Identities = 13/38 (34%), Positives = 19/38 (50%)
 Frame = -1

Query: 531 LQVCLYDIIVFWTFLSTGY*VMEAFRLRITESSKSVCF 418
           +QVCLY   +FW   S+G  V +     +   + SV F
Sbjct: 4   IQVCLYFTFIFWNGFSSGEDVQQNIAGVVYHKTDSVLF 41


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 21,039,404
Number of Sequences: 59808
Number of extensions: 428847
Number of successful extensions: 1090
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 1027
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1090
length of database: 16,821,457
effective HSP length: 80
effective length of database: 12,036,817
effective search space used: 1793485733
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -