SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brP-0035
         (750 letters)

Database: bee 
           438 sequences; 146,343 total letters

Searching......................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF015267-1|AAC38959.1|  461|Apis mellifera elongation factor-1al...   392   e-111
X52884-1|CAA37066.1|  461|Apis mellifera elongation factor 1 alp...   382   e-108
AY208278-1|AAO48970.1|  274|Apis mellifera elongation factor 1-a...   273   1e-75
EF013389-1|ABK54743.1|  172|Apis mellifera elongation factor 1-a...   240   9e-66
AF069739-1|AAC63272.2|  690|Apis mellifera translation initiatio...    33   0.004
AM050259-1|CAJ18340.1|  683|Apis mellifera putative H3K9 methylt...    24   1.3  
DQ244074-1|ABB36784.1|  517|Apis mellifera cytochrome P450 monoo...    23   3.1  
AB253417-1|BAE86928.1|  567|Apis mellifera alpha-glucosidase pro...    23   3.1  
D79208-1|BAA11466.1|  567|Apis mellifera alpha-glucosidase protein.    23   4.0  
AB253415-1|BAE86926.1|  588|Apis mellifera alpha-glucosidase pro...    21   9.3  

>AF015267-1|AAC38959.1|  461|Apis mellifera elongation factor-1alpha
           F2 protein.
          Length = 461

 Score =  392 bits (964), Expect = e-111
 Identities = 187/195 (95%), Positives = 187/195 (95%)
 Frame = +2

Query: 38  MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 217
           MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL
Sbjct: 1   MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60

Query: 218 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVXAGT 397
           DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIV AGT
Sbjct: 61  DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 120

Query: 398 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDXTEPPYSEPRFEEIKKEVCSYXKK 577
           GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMD TEPPYSE RFEEIKKEV SY KK
Sbjct: 121 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKK 180

Query: 578 IGYNPAAXAFVPIXG 622
           IGYNPAA AFVPI G
Sbjct: 181 IGYNPAAVAFVPISG 195



 Score = 58.8 bits (136), Expect = 5e-11
 Identities = 23/32 (71%), Positives = 27/32 (84%)
 Frame = +1

Query: 631 GDNMLEPSTKMPWFRGWQVERKEGKADGNALL 726
           GDNMLE S+KMPWF+GW VERKEGK +G  L+
Sbjct: 198 GDNMLEVSSKMPWFKGWTVERKEGKVEGKCLI 229



 Score = 29.1 bits (62), Expect = 0.047
 Identities = 16/46 (34%), Positives = 21/46 (45%)
 Frame = +3

Query: 612 PXVGWHGRQHVGAFNQNALVQGMAGGA*GRQSXRKCLIEXLDAXLP 749
           P  GWHG   +   ++    +G        +   KCLIE LDA LP
Sbjct: 192 PISGWHGDNMLEVSSKMPWFKGWTVERKEGKVEGKCLIEALDAILP 237


>X52884-1|CAA37066.1|  461|Apis mellifera elongation factor 1 alpha
           protein.
          Length = 461

 Score =  382 bits (941), Expect = e-108
 Identities = 182/195 (93%), Positives = 185/195 (94%)
 Frame = +2

Query: 38  MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 217
           MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL
Sbjct: 1   MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60

Query: 218 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVXAGT 397
           DKLKAERERGITIDIALWKFET+KYYVTIIDAPGHRDFIKNMITGTSQADCAVLIV AG 
Sbjct: 61  DKLKAERERGITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGI 120

Query: 398 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDXTEPPYSEPRFEEIKKEVCSYXKK 577
           GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMD T+PPYSE RFEEIKKEV SY KK
Sbjct: 121 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDMTDPPYSEARFEEIKKEVSSYIKK 180

Query: 578 IGYNPAAXAFVPIXG 622
           IGYN A+ AFVPI G
Sbjct: 181 IGYNTASVAFVPISG 195



 Score = 58.8 bits (136), Expect = 5e-11
 Identities = 22/32 (68%), Positives = 27/32 (84%)
 Frame = +1

Query: 631 GDNMLEPSTKMPWFRGWQVERKEGKADGNALL 726
           GDNMLEPS K PW++GW+VERK+G ADG  L+
Sbjct: 198 GDNMLEPSPKTPWYKGWKVERKDGNADGKTLI 229


>AY208278-1|AAO48970.1|  274|Apis mellifera elongation factor
           1-alpha protein.
          Length = 274

 Score =  273 bits (670), Expect = 1e-75
 Identities = 131/138 (94%), Positives = 131/138 (94%)
 Frame = +2

Query: 209 WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVX 388
           WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 
Sbjct: 1   WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 60

Query: 389 AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDXTEPPYSEPRFEEIKKEVCSY 568
           AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMD TEPPYSE RFEEIKKEV SY
Sbjct: 61  AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSY 120

Query: 569 XKKIGYNPAAXAFVPIXG 622
            KKIGYNPAA AFVPI G
Sbjct: 121 IKKIGYNPAAVAFVPISG 138



 Score = 58.8 bits (136), Expect = 5e-11
 Identities = 23/32 (71%), Positives = 27/32 (84%)
 Frame = +1

Query: 631 GDNMLEPSTKMPWFRGWQVERKEGKADGNALL 726
           GDNMLE S+KMPWF+GW VERKEGK +G  L+
Sbjct: 141 GDNMLEVSSKMPWFKGWTVERKEGKVEGKCLI 172



 Score = 29.1 bits (62), Expect = 0.047
 Identities = 16/46 (34%), Positives = 21/46 (45%)
 Frame = +3

Query: 612 PXVGWHGRQHVGAFNQNALVQGMAGGA*GRQSXRKCLIEXLDAXLP 749
           P  GWHG   +   ++    +G        +   KCLIE LDA LP
Sbjct: 135 PISGWHGDNMLEVSSKMPWFKGWTVERKEGKVEGKCLIEALDAILP 180


>EF013389-1|ABK54743.1|  172|Apis mellifera elongation factor
           1-alpha protein.
          Length = 172

 Score =  240 bits (588), Expect = 9e-66
 Identities = 115/122 (94%), Positives = 115/122 (94%)
 Frame = +2

Query: 257 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVXAGTGEFEAGISKNGQT 436
           DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIV AGTGEFEAGISKNGQT
Sbjct: 1   DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 60

Query: 437 REHALLAFTLGVKQLIVGVNKMDXTEPPYSEPRFEEIKKEVCSYXKKIGYNPAAXAFVPI 616
           REHALLAFTLGVKQLIVGVNKMD TEPPYSE RFEEIKKEV SY KKIGYNPAA AFVPI
Sbjct: 61  REHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPI 120

Query: 617 XG 622
            G
Sbjct: 121 SG 122



 Score = 58.8 bits (136), Expect = 5e-11
 Identities = 23/32 (71%), Positives = 27/32 (84%)
 Frame = +1

Query: 631 GDNMLEPSTKMPWFRGWQVERKEGKADGNALL 726
           GDNMLE S+KMPWF+GW VERKEGK +G  L+
Sbjct: 125 GDNMLEVSSKMPWFKGWTVERKEGKVEGKCLI 156



 Score = 29.1 bits (62), Expect = 0.047
 Identities = 16/46 (34%), Positives = 21/46 (45%)
 Frame = +3

Query: 612 PXVGWHGRQHVGAFNQNALVQGMAGGA*GRQSXRKCLIEXLDAXLP 749
           P  GWHG   +   ++    +G        +   KCLIE LDA LP
Sbjct: 119 PISGWHGDNMLEVSSKMPWFKGWTVERKEGKVEGKCLIEALDAILP 164


>AF069739-1|AAC63272.2|  690|Apis mellifera translation initiation
           factor 2 protein.
          Length = 690

 Score = 32.7 bits (71), Expect = 0.004
 Identities = 25/70 (35%), Positives = 32/70 (45%)
 Frame = +2

Query: 296 VTIIDAPGHRDFIKNMITGTSQADCAVLIVXAGTGEFEAGISKNGQTREHALLAFTLGVK 475
           VT +D PGH  FI     G    D  VL+V A  G  E       QT +   +A    V 
Sbjct: 195 VTFLDTPGHAAFISMRHRGAHITDIVVLVVAADDGVKE-------QTLQSIEMAKDAKV- 246

Query: 476 QLIVGVNKMD 505
            +IV +NK+D
Sbjct: 247 PIIVAINKID 256



 Score = 25.4 bits (53), Expect = 0.57
 Identities = 9/18 (50%), Positives = 13/18 (72%)
 Frame = +2

Query: 50  KTHINIVVIGHVDSGKST 103
           K H  + ++GHVD GK+T
Sbjct: 143 KRHPIVTIMGHVDHGKTT 160


>AM050259-1|CAJ18340.1|  683|Apis mellifera putative H3K9
           methyltransferase protein.
          Length = 683

 Score = 24.2 bits (50), Expect = 1.3
 Identities = 11/15 (73%), Positives = 11/15 (73%)
 Frame = +2

Query: 59  INIVVIGHVDSGKST 103
           INI  IGHV  GKST
Sbjct: 43  INIGTIGHVAHGKST 57


>DQ244074-1|ABB36784.1|  517|Apis mellifera cytochrome P450
           monooxygenase protein.
          Length = 517

 Score = 23.0 bits (47), Expect = 3.1
 Identities = 10/17 (58%), Positives = 12/17 (70%)
 Frame = -1

Query: 51  FSLPIFG*SRITNCV*Y 1
           FSLPIFG   I +C+ Y
Sbjct: 57  FSLPIFGTRWIFSCIGY 73


>AB253417-1|BAE86928.1|  567|Apis mellifera alpha-glucosidase
           protein.
          Length = 567

 Score = 23.0 bits (47), Expect = 3.1
 Identities = 13/34 (38%), Positives = 18/34 (52%)
 Frame = +2

Query: 281 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 382
           T KYY    D P +  FIKN+   ++ +D   LI
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLI 327


>D79208-1|BAA11466.1|  567|Apis mellifera alpha-glucosidase protein.
          Length = 567

 Score = 22.6 bits (46), Expect = 4.0
 Identities = 12/34 (35%), Positives = 18/34 (52%)
 Frame = +2

Query: 281 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 382
           T KYY    D P +  FIKN+   ++ +D   L+
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLV 327


>AB253415-1|BAE86926.1|  588|Apis mellifera alpha-glucosidase
           protein.
          Length = 588

 Score = 21.4 bits (43), Expect = 9.3
 Identities = 8/18 (44%), Positives = 12/18 (66%)
 Frame = -3

Query: 553 LLDFLKSGLTVWWFSXIH 500
           L  F++SG+T  W S I+
Sbjct: 56  LSHFIESGITAIWLSPIN 73


  Database: bee
    Posted date:  Oct 23, 2007  1:17 PM
  Number of letters in database: 146,343
  Number of sequences in database:  438
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 203,604
Number of Sequences: 438
Number of extensions: 3986
Number of successful extensions: 25
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 16
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 25
length of database: 146,343
effective HSP length: 56
effective length of database: 121,815
effective search space used: 23510295
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -