Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV30003
(692 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 127 8e-30
At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 100 2e-21
At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 100 2e-21
At5g25230.1 68418.m02991 elongation factor Tu family protein tra... 97 1e-20
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 71 7e-13
At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 37 0.015
At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 37 0.015
At5g13650.2 68418.m01585 elongation factor family protein contai... 34 0.078
At5g13650.1 68418.m01584 elongation factor family protein contai... 34 0.078
At5g26920.1 68418.m03210 calmodulin-binding protein similar to c... 29 2.2
At5g38660.1 68418.m04675 expressed protein similar to unknown pr... 28 5.1
At2g40070.1 68415.m04923 expressed protein 28 5.1
At1g65910.1 68414.m07479 no apical meristem (NAM) family protein... 28 6.8
At4g23820.1 68417.m03425 glycoside hydrolase family 28 protein /... 27 8.9
At4g00980.1 68417.m00132 zinc knuckle (CCHC-type) family protein... 27 8.9
At3g63180.1 68416.m07097 expressed protein 27 8.9
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 127 bits (306), Expect = 8e-30
Identities = 54/81 (66%), Positives = 67/81 (82%)
Frame = -2
Query: 511 LEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGG 332
+EIQ PE A+GGIY VLN++RGHVFEE Q GTP++ +KAYLPV ESFGF++ LR+ T G
Sbjct: 732 VEIQAPEGALGGIYSVLNQKRGHVFEEMQRPGTPLYNIKAYLPVVESFGFSSQLRAATSG 791
Query: 331 QAFPQCVFDHWQVLPGDPCEP 269
QAFPQCVFDHW+++ DP EP
Sbjct: 792 QAFPQCVFDHWEMMSSDPLEP 812
Score = 93.9 bits (223), Expect = 9e-20
Identities = 39/60 (65%), Positives = 51/60 (85%)
Frame = -3
Query: 690 AKEGVMAEENLRGVRFNIYDVTLHTDAIHRGGGQIIPTTRRCLYACLLTAQPRLMEPVYL 511
+KEG +AEEN+RG+ F + DV LH+DAIHRGGGQ+IPT RR +YA +TA+PRL+EPVY+
Sbjct: 672 SKEGPLAEENMRGICFEVCDVVLHSDAIHRGGGQVIPTARRVIYASQITAKPRLLEPVYM 731
>At1g06220.2 68414.m00656 elongation factor Tu family protein similar
to Cryptosporidium parvum elongation factor-2 GB:U21667
GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 99.5 bits (237), Expect = 2e-21
Identities = 47/80 (58%), Positives = 56/80 (70%)
Frame = -2
Query: 511 LEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGG 332
+EIQ P V IY VL+RRRGHV + GTP +IVKA+LPV ESFGF DLR +T G
Sbjct: 848 VEIQTPIDCVTAIYTVLSRRRGHVTSDVPQPGTPAYIVKAFLPVIESFGFETDLRYHTQG 907
Query: 331 QAFPQCVFDHWQVLPGDPCE 272
QAF VFDHW ++PGDP +
Sbjct: 908 QAFCLSVFDHWAIVPGDPLD 927
Score = 70.1 bits (164), Expect = 1e-12
Identities = 30/59 (50%), Positives = 40/59 (67%)
Frame = -3
Query: 690 AKEGVMAEENLRGVRFNIYDVTLHTDAIHRGGGQIIPTTRRCLYACLLTAQPRLMEPVY 514
A+EG + +E +R V+F I D + + +HRG GQ+IPT RR Y+ L A PRLMEPVY
Sbjct: 788 AREGPLCDEPIRNVKFKIVDARIAPEPLHRGSGQMIPTARRVAYSAFLMATPRLMEPVY 846
>At1g06220.1 68414.m00655 elongation factor Tu family protein similar
to Cryptosporidium parvum elongation factor-2 GB:U21667
GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 99.5 bits (237), Expect = 2e-21
Identities = 47/80 (58%), Positives = 56/80 (70%)
Frame = -2
Query: 511 LEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGG 332
+EIQ P V IY VL+RRRGHV + GTP +IVKA+LPV ESFGF DLR +T G
Sbjct: 848 VEIQTPIDCVTAIYTVLSRRRGHVTSDVPQPGTPAYIVKAFLPVIESFGFETDLRYHTQG 907
Query: 331 QAFPQCVFDHWQVLPGDPCE 272
QAF VFDHW ++PGDP +
Sbjct: 908 QAFCLSVFDHWAIVPGDPLD 927
Score = 70.1 bits (164), Expect = 1e-12
Identities = 30/59 (50%), Positives = 40/59 (67%)
Frame = -3
Query: 690 AKEGVMAEENLRGVRFNIYDVTLHTDAIHRGGGQIIPTTRRCLYACLLTAQPRLMEPVY 514
A+EG + +E +R V+F I D + + +HRG GQ+IPT RR Y+ L A PRLMEPVY
Sbjct: 788 AREGPLCDEPIRNVKFKIVDARIAPEPLHRGSGQMIPTARRVAYSAFLMATPRLMEPVY 846
>At5g25230.1 68418.m02991 elongation factor Tu family protein
translation Elongation Factor 2, Schizosaccharomyces
pombe, PIR:T39902
Length = 973
Score = 97.1 bits (231), Expect = 1e-20
Identities = 46/80 (57%), Positives = 56/80 (70%)
Frame = -2
Query: 511 LEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGG 332
+EIQ P V IY VL+RRRG+V + GTP +IVKA+LPV ESFGF DLR +T G
Sbjct: 834 VEIQTPIDCVTAIYTVLSRRRGYVTSDVPQPGTPAYIVKAFLPVIESFGFETDLRYHTQG 893
Query: 331 QAFPQCVFDHWQVLPGDPCE 272
QAF VFDHW ++PGDP +
Sbjct: 894 QAFCLSVFDHWAIVPGDPLD 913
Score = 70.1 bits (164), Expect = 1e-12
Identities = 30/59 (50%), Positives = 40/59 (67%)
Frame = -3
Query: 690 AKEGVMAEENLRGVRFNIYDVTLHTDAIHRGGGQIIPTTRRCLYACLLTAQPRLMEPVY 514
A+EG + +E +R V+F I D + + +HRG GQ+IPT RR Y+ L A PRLMEPVY
Sbjct: 774 AREGPLCDEPIRNVKFKIVDARIAPEPLHRGSGQMIPTARRVAYSAFLMATPRLMEPVY 832
>At3g22980.1 68416.m02898 elongation factor Tu family protein similar
to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 70.9 bits (166), Expect = 7e-13
Identities = 31/69 (44%), Positives = 43/69 (62%)
Frame = -2
Query: 484 VGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQAFPQCVFD 305
+G +Y VL+RRR + +E G+ +F V AY+PV+ESFGF +LR T G A V
Sbjct: 890 LGPMYAVLSRRRARILKEEMQEGSSLFTVHAYVPVSESFGFADELRKGTSGGASALMVLS 949
Query: 304 HWQVLPGDP 278
HW++L DP
Sbjct: 950 HWEMLEEDP 958
>At2g45030.1 68415.m05606 mitochondrial elongation factor, putative
similar to SP|P25039 Elongation factor G 1,
mitochondrial precursor (mEF-G-1) {Saccharomyces
cerevisiae}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03764: Elongation factor
G domain IV, PF00679: Elongation factor G C-terminus
Length = 754
Score = 36.7 bits (81), Expect = 0.015
Identities = 18/61 (29%), Positives = 33/61 (54%)
Frame = -2
Query: 511 LEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGG 332
+E++ P G + G +N+R+G + Q ++ A +P+N FG++ LRS T G
Sbjct: 664 VELKVPTEFQGTVAGDINKRKGIIVGNDQEGDDS--VITANVPLNNMFGYSTSLRSMTQG 721
Query: 331 Q 329
+
Sbjct: 722 K 722
>At1g45332.1 68414.m05195 mitochondrial elongation factor, putative
similar to mitochondrial elongation factor GI:3917 from
[Saccharomyces cerevisiae]
Length = 754
Score = 36.7 bits (81), Expect = 0.015
Identities = 18/61 (29%), Positives = 33/61 (54%)
Frame = -2
Query: 511 LEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGG 332
+E++ P G + G +N+R+G + Q ++ A +P+N FG++ LRS T G
Sbjct: 664 VELKVPTEFQGTVAGDINKRKGIIVGNDQEGDDS--VITANVPLNNMFGYSTSLRSMTQG 721
Query: 331 Q 329
+
Sbjct: 722 K 722
>At5g13650.2 68418.m01585 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain,PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 676
Score = 34.3 bits (75), Expect = 0.078
Identities = 18/75 (24%), Positives = 33/75 (44%)
Frame = -2
Query: 505 IQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQA 326
++ PE +G + +L +RRG +F+ V ++ +P G + + + G A
Sbjct: 483 VEVPEAHMGPVVELLGKRRGQMFDMQGVGSEGTTFLRYKIPTRGLLGLRNAILTASRGTA 542
Query: 325 FPQCVFDHWQVLPGD 281
VFD + GD
Sbjct: 543 ILNTVFDSYGPWAGD 557
>At5g13650.1 68418.m01584 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain,PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 675
Score = 34.3 bits (75), Expect = 0.078
Identities = 18/75 (24%), Positives = 33/75 (44%)
Frame = -2
Query: 505 IQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQA 326
++ PE +G + +L +RRG +F+ V ++ +P G + + + G A
Sbjct: 482 VEVPEAHMGPVVELLGKRRGQMFDMQGVGSEGTTFLRYKIPTRGLLGLRNAILTASRGTA 541
Query: 325 FPQCVFDHWQVLPGD 281
VFD + GD
Sbjct: 542 ILNTVFDSYGPWAGD 556
>At5g26920.1 68418.m03210 calmodulin-binding protein similar to
calmodulin-binding protein TCB60 GI:1698548 from
[Nicotiana tabacum]
Length = 492
Score = 29.5 bits (63), Expect = 2.2
Identities = 21/82 (25%), Positives = 37/82 (45%), Gaps = 2/82 (2%)
Frame = +1
Query: 433 LRKRDHDVCSAHRRYHPQLLQDTEFPKIYRLHETGLSS*QTCIQASSSCWNDLATTSMDG 612
L K D SA R ++L +F ++Y ++ L + S WN + + +MD
Sbjct: 175 LEKIAKDGVSATRLAERKILTVKDFRRLYTVNRNELHN-IIGAGVSKKTWNTIVSHAMDC 233
Query: 613 I--SMECYIIDVESNTTQILFS 672
+ ECYI + + +LF+
Sbjct: 234 VLDETECYIYNANTPGVTLLFN 255
>At5g38660.1 68418.m04675 expressed protein similar to unknown
protein (pir||S75762)
Length = 286
Score = 28.3 bits (60), Expect = 5.1
Identities = 15/56 (26%), Positives = 33/56 (58%), Gaps = 4/56 (7%)
Frame = +2
Query: 218 SILSSFSFPVQRLGLALRFARVSREDLPMVEYA----LREGLSAGVGTQIGSKTER 373
+I+ ++ FP+ +G+AL++A + + +P + Y+ LRE + + TQ+ + R
Sbjct: 126 AIMLTYGFPLSIIGMALKYAEL--KPVPCLSYSDAVKLRESCATPILTQVRNDVTR 179
>At2g40070.1 68415.m04923 expressed protein
Length = 607
Score = 28.3 bits (60), Expect = 5.1
Identities = 18/47 (38%), Positives = 22/47 (46%), Gaps = 1/47 (2%)
Frame = -3
Query: 342 TPADRPSRSAYSTIGRSSLETRANL-RASPKRCTGNEKEERIEGRSP 205
TP RP+ TI RSS TR + AS T N +I+ SP
Sbjct: 297 TPTSRPTLPPSKTISRSSTPTRRPIASASAATTTANPTISQIKPSSP 343
>At1g65910.1 68414.m07479 no apical meristem (NAM) family protein
similar to jasmonic acid 2 GI:6175246 from [Lycopersicon
esculentum]; similar to NAC2 (GI:6456751) {Arabidopsis
thaliana}
Length = 631
Score = 27.9 bits (59), Expect = 6.8
Identities = 15/58 (25%), Positives = 29/58 (50%), Gaps = 2/58 (3%)
Frame = +2
Query: 230 SFSFPVQRLGLALRFARVSREDLPMVEYALREGLSAG--VGTQIGSKTERLIDR*VGL 397
+ S+P ++ +AL AR+ LP V EGL+ G + + T+ ++ + + L
Sbjct: 249 TISYPPSKVDIALECARLQNRMLPPVPPLYVEGLTHNEYFGNNVANDTDEMLSKIIAL 306
>At4g23820.1 68417.m03425 glycoside hydrolase family 28 protein /
polygalacturonase (pectinase) family protein weak
similarity to polygalacturonase PG1 [Glycine max]
GI:5669846; contains PF00295: Glycosyl hydrolases family
28
Length = 444
Score = 27.5 bits (58), Expect = 8.9
Identities = 11/22 (50%), Positives = 17/22 (77%), Gaps = 1/22 (4%)
Frame = -1
Query: 452 SWSRFRRVPGGRY-TYVHCEGL 390
S+ R R +PGGRY +++H +GL
Sbjct: 123 SYGRGRELPGGRYMSFIHGDGL 144
>At4g00980.1 68417.m00132 zinc knuckle (CCHC-type) family protein
contains Pfam domain, PF00098: Zinc knuckle
Length = 488
Score = 27.5 bits (58), Expect = 8.9
Identities = 10/21 (47%), Positives = 15/21 (71%)
Frame = -3
Query: 255 KRCTGNEKEERIEGRSPRLNS 193
K C G++ +ER G+S R+NS
Sbjct: 449 KHCWGSKSDERASGKSNRINS 469
>At3g63180.1 68416.m07097 expressed protein
Length = 978
Score = 27.5 bits (58), Expect = 8.9
Identities = 10/35 (28%), Positives = 21/35 (60%)
Frame = -3
Query: 393 PTYLSMSRSVLLPICVPTPADRPSRSAYSTIGRSS 289
P ++ + L P+ VP+ + RP++S + +G +S
Sbjct: 539 PAFIYPANHHLQPVMVPSKSSRPTKSPHLAVGLAS 573
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,082,314
Number of Sequences: 28952
Number of extensions: 333647
Number of successful extensions: 777
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 745
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 777
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1477286152
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -