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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdV10416
         (765 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g14190.1 68414.m01679 glucose-methanol-choline (GMC) oxidored...    55   6e-08
At1g14185.1 68414.m01678 glucose-methanol-choline (GMC) oxidored...    55   6e-08
At3g56060.1 68416.m06229 glucose-methanol-choline (GMC) oxidored...    54   1e-07
At5g51950.1 68418.m06447 glucose-methanol-choline (GMC) oxidored...    53   2e-07
At1g12570.1 68414.m01459 glucose-methanol-choline (GMC) oxidored...    53   2e-07
At1g73050.1 68414.m08447 (R)-mandelonitrile lyase, putative / (R...    52   3e-07
At1g72970.1 68414.m08439 glucose-methanol-choline (GMC) oxidored...    50   2e-06
At5g51930.1 68418.m06442 glucose-methanol-choline (GMC) oxidored...    49   4e-06
At1g22220.1 68414.m02778 F-box family protein contains F-box dom...    29   4.5  
At2g05120.1 68415.m00539 expressed protein                             28   5.9  

>At1g14190.1 68414.m01679 glucose-methanol-choline (GMC)
           oxidoreductase family protein similar to mandelonitrile
           lyase from Prunus serotina [SP|P52706, SP|P52707];
           contains Pfam profile PF00732 GMC oxidoreductase
          Length = 501

 Score = 54.8 bits (126), Expect = 6e-08
 Identities = 26/49 (53%), Positives = 36/49 (73%), Gaps = 1/49 (2%)
 Frame = +3

Query: 561 RKEVVVSAGAINSPQLLLLSGIGPRKHLEDLNIEVKADL-PVGENLQDH 704
           R EV+++AGA+ SPQ+LLLSGIGP  HL+D +I V  +L  VG  + D+
Sbjct: 241 RGEVILAAGALGSPQILLLSGIGPENHLKDFDIPVIVNLKEVGRKMSDN 289



 Score = 29.9 bits (64), Expect = 1.9
 Identities = 25/63 (39%), Positives = 33/63 (52%)
 Frame = +2

Query: 50  RGKVLGGSSSINLMFYVRGNKADYDEWAADGNEGWSFEDVLPYFKKSESFMGKFDAEATK 229
           RG+VLGGSS+IN  FY R +    DE+      GW    V   +K  ES +  F  E T+
Sbjct: 88  RGRVLGGSSAINGGFYSRAS----DEFVK--KAGWDKGLVQESYKWVESKV-VFMPELTQ 140

Query: 230 YHS 238
           + S
Sbjct: 141 WQS 143


>At1g14185.1 68414.m01678 glucose-methanol-choline (GMC)
           oxidoreductase family protein similar to mandelonitrile
           lyase from Prunus serotina [SP|P52706, SP|P52707];
           contains Pfam profile PF00732 GMC oxidoreductase
          Length = 503

 Score = 54.8 bits (126), Expect = 6e-08
 Identities = 26/49 (53%), Positives = 36/49 (73%), Gaps = 1/49 (2%)
 Frame = +3

Query: 561 RKEVVVSAGAINSPQLLLLSGIGPRKHLEDLNIEVKADL-PVGENLQDH 704
           R EV+++AGA+ SPQ+LLLSGIGP  HL D +I V  +L  VG+ + D+
Sbjct: 261 RGEVILAAGALGSPQILLLSGIGPENHLNDFDIPVIVNLKEVGKQMSDN 309



 Score = 31.9 bits (69), Expect = 0.48
 Identities = 25/63 (39%), Positives = 34/63 (53%)
 Frame = +2

Query: 50  RGKVLGGSSSINLMFYVRGNKADYDEWAADGNEGWSFEDVLPYFKKSESFMGKFDAEATK 229
           RG+VLGGSS+IN  FY R +    DE+      GW  + V   +K  ES +  F  E T+
Sbjct: 109 RGRVLGGSSAINGGFYSRAS----DEFVK--KAGWDKDLVQESYKWVESKV-VFMPELTR 161

Query: 230 YHS 238
           + S
Sbjct: 162 WQS 164


>At3g56060.1 68416.m06229 glucose-methanol-choline (GMC)
           oxidoreductase family protein similar to mandelonitrile
           lyase from Prunus serotina [SP|P52706, SP|P52707];
           contains Pfam profile PF00732 GMC oxidoreductase
          Length = 577

 Score = 53.6 bits (123), Expect = 1e-07
 Identities = 29/58 (50%), Positives = 37/58 (63%), Gaps = 2/58 (3%)
 Frame = +3

Query: 567 EVVVSAGAINSPQLLLLSGIGPRKHLEDLNIE-VKADLP-VGENLQDHLFVPVFYTKP 734
           EV++SAGAI SPQLL+LSG+GP  HL    +  V  D P VG+ + D+   PVF   P
Sbjct: 274 EVILSAGAIASPQLLMLSGVGPAAHLAAYRVNPVIVDQPMVGQGMGDNPMNPVFIPSP 331


>At5g51950.1 68418.m06447 glucose-methanol-choline (GMC)
           oxidoreductase family protein similar to mandelonitrile
           lyase from Prunus serotina [SP|P52706, SP|P52707];
           contains Pfam profile PF00732 GMC oxidoreductase
          Length = 586

 Score = 53.2 bits (122), Expect = 2e-07
 Identities = 34/80 (42%), Positives = 48/80 (60%), Gaps = 2/80 (2%)
 Frame = +3

Query: 501 YQQVSGVLLNKGGRDIAVNVRKEVVVSAGAINSPQLLLLSGIGPRKHLEDLNIE-VKADL 677
           +Q  +GVL +K   ++  N   EV++SAGAI SPQLL+LSGIGP  HL    I+ +  D 
Sbjct: 253 FQDANGVL-HKA--ELEKNSMNEVILSAGAIGSPQLLMLSGIGPAAHLAAHGIKPLVLDH 309

Query: 678 P-VGENLQDHLFVPVFYTKP 734
           P VG+ + D+    +F   P
Sbjct: 310 PMVGQGMGDNPMNAIFIPSP 329


>At1g12570.1 68414.m01459 glucose-methanol-choline (GMC)
           oxidoreductase family protein similar to mandelonitrile
           lyase from Prunus serotina [SP|P52706, SP|P52707];
           contains Pfam profile PF00732 GMC oxidoreductase
          Length = 572

 Score = 53.2 bits (122), Expect = 2e-07
 Identities = 26/57 (45%), Positives = 36/57 (63%), Gaps = 1/57 (1%)
 Frame = +3

Query: 567 EVVVSAGAINSPQLLLLSGIGPRKHLEDLNIEVKADLP-VGENLQDHLFVPVFYTKP 734
           E+++SAG + SPQLL+LSG+GP   L+  NI V  D P VG+ + D+    VF   P
Sbjct: 272 EIILSAGTLGSPQLLMLSGVGPSAQLQAQNITVVMDQPHVGQGMYDNPMNAVFVPSP 328


>At1g73050.1 68414.m08447 (R)-mandelonitrile lyase, putative /
           (R)-oxynitrilase, putative similar to mandelonitrile
           lyase from Prunus serotina [SP|P52706, SP|52707];
           contains Pfam protile PF00732 GMC oxidoreductase
          Length = 552

 Score = 52.4 bits (120), Expect = 3e-07
 Identities = 27/49 (55%), Positives = 34/49 (69%), Gaps = 1/49 (2%)
 Frame = +3

Query: 561 RKEVVVSAGAINSPQLLLLSGIGPRKHLEDLNIEVKADLP-VGENLQDH 704
           R EV++SAGA+ SPQLL LSGIGPR +L    I V  D P VG+ + D+
Sbjct: 282 RGEVILSAGALGSPQLLFLSGIGPRSYLSTWGIPVALDQPHVGDFVYDN 330



 Score = 32.7 bits (71), Expect = 0.28
 Identities = 14/25 (56%), Positives = 19/25 (76%)
 Frame = +2

Query: 50  RGKVLGGSSSINLMFYVRGNKADYD 124
           RG+VLGGSS+IN  FY R +K  ++
Sbjct: 126 RGRVLGGSSAINAGFYSRADKQFFE 150


>At1g72970.1 68414.m08439 glucose-methanol-choline (GMC)
           oxidoreductase family protein similar to mandelonitrile
           lyase from Prunus serotina [SP|P52706, SP|P52707];
           contains Pfam profile PF00732 GMC oxidoreductase
          Length = 594

 Score = 50.0 bits (114), Expect = 2e-06
 Identities = 32/72 (44%), Positives = 49/72 (68%), Gaps = 6/72 (8%)
 Frame = +3

Query: 507 QVSGVLLN--KGGRDIAV-NVRK--EVVVSAGAINSPQLLLLSGIGPRKHLEDLNIEVKA 671
           +V+GV+    KG +  A+ + RK  EV++S+GAI SPQ+L+LSGIGP+K L+ L I V  
Sbjct: 263 RVTGVIFKDEKGNQHQALLSNRKGSEVILSSGAIGSPQMLMLSGIGPKKELQRLKIPVVL 322

Query: 672 DLP-VGENLQDH 704
           +   VG+ + D+
Sbjct: 323 ENEHVGKGMADN 334


>At5g51930.1 68418.m06442 glucose-methanol-choline (GMC)
           oxidoreductase family protein similar to mandelonitrile
           lyase from Prunus serotina [SP|P52706, SP|P52707];
           contains Pfam profile PF00732 GMC oxidoreductase
          Length = 582

 Score = 48.8 bits (111), Expect = 4e-06
 Identities = 25/56 (44%), Positives = 37/56 (66%), Gaps = 2/56 (3%)
 Frame = +3

Query: 543 DIAVNVRKEVVVSAGAINSPQLLLLSGIGPRKHLEDLNIE-VKADLP-VGENLQDH 704
           ++A     EV++SAGA+ SPQLL+LSG+GP  HLE   +  +  D P VG+ + D+
Sbjct: 285 NLANKATNEVILSAGALGSPQLLMLSGVGPAVHLEAHGVNPLVLDQPMVGQGMADN 340


>At1g22220.1 68414.m02778 F-box family protein contains F-box domain
           Pfam:PF00646
          Length = 314

 Score = 28.7 bits (61), Expect = 4.5
 Identities = 13/44 (29%), Positives = 20/44 (45%)
 Frame = -3

Query: 706 KWSCKFSPTGKSALTSMFRSSKCLRGPIPESNNSCGELIAPAET 575
           KW  +F  T KS +   FRS+  +  P+     S  E +   +T
Sbjct: 122 KWKAEFGKTLKSCVIVAFRSAGTVSSPVAVEGESDAEFVTGLKT 165


>At2g05120.1 68415.m00539 expressed protein 
          Length = 1234

 Score = 28.3 bits (60), Expect = 5.9
 Identities = 14/35 (40%), Positives = 18/35 (51%), Gaps = 4/35 (11%)
 Frame = +2

Query: 107  NKADYDEW----AADGNEGWSFEDVLPYFKKSESF 199
            N AD D+W     A  NEGWS E+ L   + +  F
Sbjct: 1122 NAADQDDWDRHHQASTNEGWSEEETLQNLRNTALF 1156


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,426,172
Number of Sequences: 28952
Number of extensions: 374290
Number of successful extensions: 1077
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1030
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1077
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1712086600
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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