Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= tesV0068.Seq
(978 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g08300.1 68418.m00977 succinyl-CoA ligase [GDP-forming] alpha... 49 5e-06
At5g23250.1 68418.m02720 succinyl-CoA ligase [GDP-forming] alpha... 45 9e-05
At1g14685.3 68414.m01750 expressed protein 29 4.7
At1g14685.2 68414.m01749 expressed protein 29 4.7
At1g14685.1 68414.m01748 expressed protein 29 4.7
At1g13830.1 68414.m01623 beta-1,3-glucanase-related similar to b... 29 4.7
At3g17470.1 68416.m02232 RelA/SpoT domain-containing protein / c... 29 6.2
At1g29380.1 68414.m03592 hypothetical protein 29 6.2
>At5g08300.1 68418.m00977 succinyl-CoA ligase [GDP-forming]
alpha-chain, mitochondrial, putative / succinyl-CoA
synthetase, alpha chain, putative / SCS-alpha, putative
identical to SP|P53586 Succinyl-CoA ligase [GDP-forming]
alpha-chain, mitochondrial precursor (EC 6.2.1.4)
(Succinyl-CoA synthetase, alpha chain) (SCS-alpha)
{Arabidopsis thaliana}; strong similarity to SP|P13086
Succinyl-CoA ligase [GDP-forming] alpha-chain,
mitochondrial precursor {Rattus norvegicus}; contains
Pfam profiles PF00549: CoA-ligase, PF02629: CoA binding
domain
Length = 347
Score = 48.8 bits (111), Expect = 5e-06
Identities = 27/79 (34%), Positives = 37/79 (46%)
Frame = +3
Query: 336 KLLERVSPKKAGTEHLGXPGFGXXXXXXXXXXXXXXXIDVPPPGXAAGXVXXXXAXMPSV 515
K++ V+PKK GTEHLG P F I VP P AA + A + +
Sbjct: 83 KMVAGVTPKKGGTEHLGLPVFNSVAEAKADTKANASVIYVPAPFAAAAIMEGIEAELDLI 142
Query: 516 XALQKVCHRHXXLRVKHAL 572
+ + +H +RVKHAL
Sbjct: 143 VCITEGIPQHDMVRVKHAL 161
Score = 36.7 bits (81), Expect = 0.023
Identities = 14/23 (60%), Positives = 18/23 (78%)
Frame = +1
Query: 259 TKVXVQGFTGKQGTFHSXQALDY 327
T+V QG TGK GTFH+ QA++Y
Sbjct: 58 TRVLCQGITGKNGTFHTEQAIEY 80
Score = 36.3 bits (80), Expect = 0.031
Identities = 29/85 (34%), Positives = 36/85 (42%)
Frame = +1
Query: 589 SKLVGPNCPGIIAPEECXNWXXASWQSXXXXXHXALVSRSGXIEPNEACHXTTYYWA*AK 768
++L+GPNCPGII P EC + +VSRSG + EA TT
Sbjct: 167 TRLIGPNCPGIIKPGECKIGIMPGY--IHKPGKIGIVSRSGTL-TYEAVFQTTAVGLGQS 223
Query: 769 LLWCRXWRGXPSTGTRLSLTCXEVF 843
C G P GT + C E F
Sbjct: 224 T--CVGIGGDPFNGTNF-VDCLEKF 245
>At5g23250.1 68418.m02720 succinyl-CoA ligase [GDP-forming]
alpha-chain, mitochondrial, putative / succinyl-CoA
synthetase, alpha chain, putative / SCS-alpha, putative
similar to SP|P36967 Succinyl-CoA ligase [GDP-forming]
alpha-chain, mitochondrial precursor (EC 6.2.1.4)
(Succinyl-CoA synthetase, alpha chain) (SCS-alpha)
{Dictyostelium discoideum}; contains Pfam profiles
PF00549: CoA-ligase, PF02629: CoA binding domain
Length = 341
Score = 44.8 bits (101), Expect = 9e-05
Identities = 26/79 (32%), Positives = 36/79 (45%)
Frame = +3
Query: 336 KLLERVSPKKAGTEHLGXPGFGXXXXXXXXXXXXXXXIDVPPPGXAAGXVXXXXAXMPSV 515
K++ V+PKK GTEHLG P F I VP P AA + A + +
Sbjct: 78 KMVAGVTPKKGGTEHLGLPVFNTVAEAKAETKANASVIYVPAPFAAAAIMEGLAAELDLI 137
Query: 516 XALQKVCHRHXXLRVKHAL 572
+ + +H +RVK AL
Sbjct: 138 VCITEGIPQHDMVRVKAAL 156
Score = 36.7 bits (81), Expect = 0.023
Identities = 14/23 (60%), Positives = 18/23 (78%)
Frame = +1
Query: 259 TKVXVQGFTGKQGTFHSXQALDY 327
T+V QG TGK GTFH+ QA++Y
Sbjct: 53 TRVICQGITGKNGTFHTEQAIEY 75
Score = 36.3 bits (80), Expect = 0.031
Identities = 29/85 (34%), Positives = 36/85 (42%)
Frame = +1
Query: 589 SKLVGPNCPGIIAPEECXNWXXASWQSXXXXXHXALVSRSGXIEPNEACHXTTYYWA*AK 768
++L+GPNCPGII P EC + +VSRSG + EA TT
Sbjct: 162 TRLIGPNCPGIIKPGECKIGIMPGY--IHKPGKIGIVSRSGTL-TYEAVFQTTAVGLGQS 218
Query: 769 LLWCRXWRGXPSTGTRLSLTCXEVF 843
C G P GT + C E F
Sbjct: 219 T--CVGIGGDPFNGTNF-VDCLEKF 240
>At1g14685.3 68414.m01750 expressed protein
Length = 279
Score = 29.1 bits (62), Expect = 4.7
Identities = 12/29 (41%), Positives = 14/29 (48%)
Frame = +1
Query: 583 DXSKLVGPNCPGIIAPEECXNWXXASWQS 669
D S L P C AP++C W WQS
Sbjct: 182 DISGLPVPICTCTGAPQQCYRWGCGGWQS 210
>At1g14685.2 68414.m01749 expressed protein
Length = 279
Score = 29.1 bits (62), Expect = 4.7
Identities = 12/29 (41%), Positives = 14/29 (48%)
Frame = +1
Query: 583 DXSKLVGPNCPGIIAPEECXNWXXASWQS 669
D S L P C AP++C W WQS
Sbjct: 182 DISGLPVPICTCTGAPQQCYRWGCGGWQS 210
>At1g14685.1 68414.m01748 expressed protein
Length = 279
Score = 29.1 bits (62), Expect = 4.7
Identities = 12/29 (41%), Positives = 14/29 (48%)
Frame = +1
Query: 583 DXSKLVGPNCPGIIAPEECXNWXXASWQS 669
D S L P C AP++C W WQS
Sbjct: 182 DISGLPVPICTCTGAPQQCYRWGCGGWQS 210
>At1g13830.1 68414.m01623 beta-1,3-glucanase-related similar to
beta-1,3-glucanase-like protein (GI:14279169) [Olea
europaea] similar to Glucan endo-1,3-beta-glucosidase
precursor (EC 3.2.1.39) ((1-3)-beta- glucan
endohydrolase) ((1-3)-beta-glucanase) (Beta-1,3-
endoglucanase) (Swiss-Prot:P52409) [Triticum aestivum]
Length = 197
Score = 29.1 bits (62), Expect = 4.7
Identities = 20/64 (31%), Positives = 22/64 (34%)
Frame = -3
Query: 478 PAAXPGGGTSITEALAPVPAFASLTVPNPGLPRCSVPAFFGDTRSNNFSTXSQGLVXCGR 299
P+ P GT+ T P P P PG P P FG T N S G G
Sbjct: 117 PSTTPPTGTTPTNGTTPFPG-----TPFPGTPFPGTPPVFGPTGVFNPSNPGSGASSLGT 171
Query: 298 YPAF 287
F
Sbjct: 172 SSVF 175
>At3g17470.1 68416.m02232 RelA/SpoT domain-containing protein /
calcium-binding EF-hand family protein contains
INTERPRO:IPR002048 calcium-binding EF-hand domain, Pfam
profile PF04607: Region found in RelA / SpoT proteins
Length = 583
Score = 28.7 bits (61), Expect = 6.2
Identities = 11/31 (35%), Positives = 17/31 (54%)
Frame = -2
Query: 215 CRMQIVHCRVFSPSLNLERTRHWHSHCRIID 123
CR Q++H R++S L R R W+ + D
Sbjct: 14 CRSQVLHRRLYSIQLIQRRRRRWNPRSEVED 44
>At1g29380.1 68414.m03592 hypothetical protein
Length = 228
Score = 28.7 bits (61), Expect = 6.2
Identities = 20/62 (32%), Positives = 24/62 (38%)
Frame = -3
Query: 520 AXTEGIXAXIXXTXPAAXPGGGTSITEALAPVPAFASLTVPNPGLPRCSVPAFFGDTRSN 341
A +G I P A PGG T+ P P SLT P P P P G T +
Sbjct: 28 AQGQGGQGDIPVVNPTA-PGGSTTTPTITQPSPP--SLTFPGPTTPTGGYPPLDGTTPTG 84
Query: 340 NF 335
+
Sbjct: 85 GY 86
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,136,902
Number of Sequences: 28952
Number of extensions: 294992
Number of successful extensions: 499
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 475
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 494
length of database: 12,070,560
effective HSP length: 81
effective length of database: 9,725,448
effective search space used: 2373009312
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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