Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= psV30186.Seq
(767 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha ... 154 5e-38
At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha ... 154 5e-38
At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha ... 154 5e-38
At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha ... 154 5e-38
At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,... 102 3e-22
At5g10630.1 68418.m01231 elongation factor 1-alpha, putative / E... 96 3e-20
At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA) ide... 70 2e-12
At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,... 67 1e-11
At4g18330.2 68417.m02719 eukaryotic translation initiation facto... 40 0.001
At4g18330.1 68417.m02718 eukaryotic translation initiation facto... 40 0.001
At1g04170.1 68414.m00407 eukaryotic translation initiation facto... 38 0.007
At2g18720.1 68415.m02180 eukaryotic translation initiation facto... 38 0.010
At5g39900.1 68418.m04839 GTP-binding protein LepA, putative GTP-... 34 0.091
At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 34 0.12
At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 34 0.12
At4g11160.1 68417.m01808 translation initiation factor IF-2, mit... 33 0.28
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 33 0.28
At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 33 0.28
At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 31 0.64
At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 31 0.64
At1g76720.1 68414.m08929 eukaryotic translation initiation facto... 31 0.85
At1g21160.1 68414.m02646 eukaryotic translation initiation facto... 31 0.85
At5g08650.1 68418.m01029 GTP-binding protein LepA, putative 31 1.1
At1g76825.1 68414.m08940 eukaryotic translation initiation facto... 30 1.5
At1g76810.1 68414.m08938 eukaryotic translation initiation facto... 30 1.5
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 29 2.6
At5g44785.2 68418.m05490 expressed protein contains Pfam PF05329... 29 4.5
At5g44785.1 68418.m05489 expressed protein contains Pfam PF05329... 29 4.5
At1g62660.1 68414.m07071 beta-fructosidase (BFRUCT3) / beta-fruc... 29 4.5
At3g20440.1 68416.m02588 glycoside hydrolase family 13 protein s... 28 6.0
At1g22730.1 68414.m02840 MA3 domain-containing protein contains ... 28 6.0
At1g17220.1 68414.m02098 translation initiation factor IF-2, chl... 28 6.0
At2g28000.1 68415.m03393 RuBisCO subunit binding-protein alpha s... 28 7.9
>At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha
identical to SWISS-PROT:P13905 elongation factor 1-alpha
(EF-1-alpha) [Arabidopsis thaliana]
Length = 449
Score = 154 bits (374), Expect = 5e-38
Identities = 72/85 (84%), Positives = 78/85 (91%)
Frame = +3
Query: 255 TIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNG 434
TIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + TG FEAGISK+G
Sbjct: 72 TIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDG 131
Query: 435 QTREHALLAFTLGVKQLIVGVNKMD 509
QTREHALLAFTLGVKQ+I NKMD
Sbjct: 132 QTREHALLAFTLGVKQMICCCNKMD 156
Score = 130 bits (313), Expect = 1e-30
Identities = 61/68 (89%), Positives = 62/68 (91%)
Frame = +1
Query: 43 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 222
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 223 DKLKAERE 246
DKLKAERE
Sbjct: 61 DKLKAERE 68
Score = 80.2 bits (189), Expect = 1e-15
Identities = 33/57 (57%), Positives = 44/57 (77%)
Frame = +2
Query: 509 STEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDXMLEPSTKMXWFXG 679
+T P YS+ R++EI KEVSSY+KK+GYNP + FVPISG+ GD M+E ST + W+ G
Sbjct: 157 ATTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWYKG 213
>At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 154 bits (374), Expect = 5e-38
Identities = 72/85 (84%), Positives = 78/85 (91%)
Frame = +3
Query: 255 TIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNG 434
TIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + TG FEAGISK+G
Sbjct: 72 TIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDG 131
Query: 435 QTREHALLAFTLGVKQLIVGVNKMD 509
QTREHALLAFTLGVKQ+I NKMD
Sbjct: 132 QTREHALLAFTLGVKQMICCCNKMD 156
Score = 130 bits (313), Expect = 1e-30
Identities = 61/68 (89%), Positives = 62/68 (91%)
Frame = +1
Query: 43 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 222
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 223 DKLKAERE 246
DKLKAERE
Sbjct: 61 DKLKAERE 68
Score = 80.2 bits (189), Expect = 1e-15
Identities = 33/57 (57%), Positives = 44/57 (77%)
Frame = +2
Query: 509 STEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDXMLEPSTKMXWFXG 679
+T P YS+ R++EI KEVSSY+KK+GYNP + FVPISG+ GD M+E ST + W+ G
Sbjct: 157 ATTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWYKG 213
>At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 154 bits (374), Expect = 5e-38
Identities = 72/85 (84%), Positives = 78/85 (91%)
Frame = +3
Query: 255 TIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNG 434
TIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + TG FEAGISK+G
Sbjct: 72 TIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDG 131
Query: 435 QTREHALLAFTLGVKQLIVGVNKMD 509
QTREHALLAFTLGVKQ+I NKMD
Sbjct: 132 QTREHALLAFTLGVKQMICCCNKMD 156
Score = 130 bits (313), Expect = 1e-30
Identities = 61/68 (89%), Positives = 62/68 (91%)
Frame = +1
Query: 43 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 222
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 223 DKLKAERE 246
DKLKAERE
Sbjct: 61 DKLKAERE 68
Score = 80.2 bits (189), Expect = 1e-15
Identities = 33/57 (57%), Positives = 44/57 (77%)
Frame = +2
Query: 509 STEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDXMLEPSTKMXWFXG 679
+T P YS+ R++EI KEVSSY+KK+GYNP + FVPISG+ GD M+E ST + W+ G
Sbjct: 157 ATTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWYKG 213
>At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 154 bits (374), Expect = 5e-38
Identities = 72/85 (84%), Positives = 78/85 (91%)
Frame = +3
Query: 255 TIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNG 434
TIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + TG FEAGISK+G
Sbjct: 72 TIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDG 131
Query: 435 QTREHALLAFTLGVKQLIVGVNKMD 509
QTREHALLAFTLGVKQ+I NKMD
Sbjct: 132 QTREHALLAFTLGVKQMICCCNKMD 156
Score = 130 bits (313), Expect = 1e-30
Identities = 61/68 (89%), Positives = 62/68 (91%)
Frame = +1
Query: 43 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 222
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 223 DKLKAERE 246
DKLKAERE
Sbjct: 61 DKLKAERE 68
Score = 80.2 bits (189), Expect = 1e-15
Identities = 33/57 (57%), Positives = 44/57 (77%)
Frame = +2
Query: 509 STEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDXMLEPSTKMXWFXG 679
+T P YS+ R++EI KEVSSY+KK+GYNP + FVPISG+ GD M+E ST + W+ G
Sbjct: 157 ATTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWYKG 213
>At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,
putative similar to EF-1-alpha-related GTP-binding
protein gi|1009232|gb|AAA79032
Length = 532
Score = 102 bits (244), Expect = 3e-22
Identities = 47/85 (55%), Positives = 60/85 (70%)
Frame = +3
Query: 255 TIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNG 434
T+++ FET TI+DAPGH+ ++ NMI+G SQAD VL+++A GEFE G + G
Sbjct: 166 TVEVGRAHFETESTRFTILDAPGHKSYVPNMISGASQADIGVLVISARKGEFETGYERGG 225
Query: 435 QTREHALLAFTLGVKQLIVGVNKMD 509
QTREH LA TLGV +LIV VNKMD
Sbjct: 226 QTREHVQLAKTLGVSKLIVVVNKMD 250
Score = 68.1 bits (159), Expect = 6e-12
Identities = 28/64 (43%), Positives = 46/64 (71%)
Frame = +1
Query: 52 EKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKL 231
+K H+N+V IGHVD+GKST G +++ G +D R I+K+EKEA++ + S+ A+++D
Sbjct: 98 KKRHLNVVFIGHVDAGKSTIGGQILFLSGQVDDRQIQKYEKEAKDKSRESWYMAYIMDTN 157
Query: 232 KAER 243
+ ER
Sbjct: 158 EEER 161
>At5g10630.1 68418.m01231 elongation factor 1-alpha, putative /
EF-1-alpha, putative contains similarity to
SWISS-PROT:Q9YAV0 elongation factor 1-alpha (EF-1-alpha)
[Aeropyrum pernix]
Length = 667
Score = 95.9 bits (228), Expect = 3e-20
Identities = 44/99 (44%), Positives = 66/99 (66%), Gaps = 1/99 (1%)
Frame = +3
Query: 255 TIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK-N 431
T+ +A+ F + +++V ++D+PGH+DF+ NMI G +QAD A+L++ A G FEAG
Sbjct: 304 TMTVAVAYFNSKRHHVVLLDSPGHKDFVPNMIAGATQADAAILVIDASVGAFEAGFDNLK 363
Query: 432 GQTREHALLAFTLGVKQLIVGVNKMDPLNHHTVSPDLRK 548
GQTREHA + GV+Q+IV +NKMD + + DL K
Sbjct: 364 GQTREHARVLRGFGVEQVIVAINKMDIVGYSKERFDLIK 402
Score = 77.4 bits (182), Expect = 1e-14
Identities = 32/63 (50%), Positives = 46/63 (73%)
Frame = +1
Query: 58 THINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKA 237
+ +N+ ++GHVDSGKST +G L++ G I ++ + K+EKEA+ GKGSF YAW LD+
Sbjct: 238 SQLNLAIVGHVDSGKSTLSGRLLHLLGRISQKQMHKYEKEAKLQGKGSFAYAWALDESAE 297
Query: 238 ERE 246
ERE
Sbjct: 298 ERE 300
Score = 31.1 bits (67), Expect = 0.85
Identities = 14/55 (25%), Positives = 30/55 (54%), Gaps = 3/55 (5%)
Frame = +2
Query: 524 YSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDXML-EPSTK--MXWFXG 679
YS+ RF+ IK+ V S+++ + +++ ++P+S ++ PS W+ G
Sbjct: 393 YSKERFDLIKQHVGSFLQSCRFKDSSLTWIPLSAMENQNLVAAPSDNRLSSWYQG 447
>At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA)
identical to SWISS-PROT:P17745 elongation factor Tu,
chloroplast precursor (EF-Tu) [Arabidopsis thaliana]
Length = 476
Score = 69.7 bits (163), Expect = 2e-12
Identities = 35/88 (39%), Positives = 53/88 (60%)
Frame = +3
Query: 255 TIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNG 434
TI+ A ++ET + +D PGH D++KNMITG +Q D A+L+V+ G
Sbjct: 129 TINTATVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGADGPMP------- 181
Query: 435 QTREHALLAFTLGVKQLIVGVNKMDPLN 518
QT+EH LLA +GV ++V +NK D ++
Sbjct: 182 QTKEHILLAKQVGVPDMVVFLNKEDQVD 209
Score = 39.1 bits (87), Expect = 0.003
Identities = 17/45 (37%), Positives = 25/45 (55%)
Frame = +1
Query: 40 KMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEK 174
K ++K H+NI IGHVD GK+T T L I +K+++
Sbjct: 72 KFERKKPHVNIGTIGHVDHGKTTLTAALTMALASIGSSVAKKYDE 116
>At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,
putative similar to mitochondrial elongation factor Tu
[Arabidopsis thaliana] gi|1149571|emb|CAA61511
Length = 454
Score = 67.3 bits (157), Expect = 1e-11
Identities = 35/85 (41%), Positives = 51/85 (60%)
Frame = +3
Query: 255 TIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNG 434
TI A ++ET+K + +D PGH D++KNMITG +Q D +L+V+ G
Sbjct: 117 TIATAHVEYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGPMP------- 169
Query: 435 QTREHALLAFTLGVKQLIVGVNKMD 509
QT+EH LLA +GV L+ +NK+D
Sbjct: 170 QTKEHILLARQVGVPSLVCFLNKVD 194
Score = 32.7 bits (71), Expect = 0.28
Identities = 12/22 (54%), Positives = 16/22 (72%)
Frame = +1
Query: 49 KEKTHINIVVIGHVDSGKSTTT 114
+ K H+N+ IGHVD GK+T T
Sbjct: 63 RNKPHVNVGTIGHVDHGKTTLT 84
>At4g18330.2 68417.m02719 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to SP|Q09130 Eukaryotic translation
initiation factor 2 gamma subunit (eIF-2- gamma)
{Schizosaccharomyces pombe}; contains Pfam profile
PF00009: Elongation factor Tu GTP binding domain;
isoform predicted to contain a TG non-consensus acceptor
splice site.
Length = 471
Score = 40.3 bits (90), Expect = 0.001
Identities = 23/78 (29%), Positives = 39/78 (50%)
Frame = +3
Query: 285 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAF 464
T + +V+ +D PGH + M+ G + D A+L++AA QT EH
Sbjct: 124 TLRRHVSCVDCPGHDILMATMLNGAAIVDGALLLIAANES------CPQPQTAEHLASVD 177
Query: 465 TLGVKQLIVGVNKMDPLN 518
+ +K +I+ NK+D +N
Sbjct: 178 MMRLKHIIILQNKIDLIN 195
>At4g18330.1 68417.m02718 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to SP|Q09130 Eukaryotic translation
initiation factor 2 gamma subunit (eIF-2- gamma)
{Schizosaccharomyces pombe}; contains Pfam profile
PF00009: Elongation factor Tu GTP binding domain;
isoform predicted to contain a TG non-consensus acceptor
splice site.
Length = 284
Score = 40.3 bits (90), Expect = 0.001
Identities = 23/78 (29%), Positives = 39/78 (50%)
Frame = +3
Query: 285 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAF 464
T + +V+ +D PGH + M+ G + D A+L++AA QT EH
Sbjct: 124 TLRRHVSCVDCPGHDILMATMLNGAAIVDGALLLIAANES------CPQPQTAEHLASVD 177
Query: 465 TLGVKQLIVGVNKMDPLN 518
+ +K +I+ NK+D +N
Sbjct: 178 MMRLKHIIILQNKIDLIN 195
>At1g04170.1 68414.m00407 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to gb|U37354 from S. pombe. ESTs
gb|T41979, gb|N37284 and gb|N37529 come from this gene
Length = 465
Score = 37.9 bits (84), Expect = 0.007
Identities = 25/81 (30%), Positives = 39/81 (48%), Gaps = 4/81 (4%)
Frame = +3
Query: 279 FETSKY----YVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTRE 446
FE SK +V+ +D PGH + M+ G + D A+L++AA QT E
Sbjct: 112 FENSKMKLLRHVSFVDCPGHDILMATMLNGAAIMDGALLLIAANE------TCPQPQTSE 165
Query: 447 HALLAFTLGVKQLIVGVNKMD 509
H + +K +I+ NK+D
Sbjct: 166 HLAAVEIMQLKHIIILQNKID 186
>At2g18720.1 68415.m02180 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative
Length = 465
Score = 37.5 bits (83), Expect = 0.010
Identities = 22/71 (30%), Positives = 35/71 (49%)
Frame = +3
Query: 297 YVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGV 476
+V+ +D PGH + M+ G + D A+LI+AA QT EH + +
Sbjct: 120 HVSFVDCPGHDILMATMLNGAAIMDGALLIIAANE------TCPQPQTAEHLASVDMMHL 173
Query: 477 KQLIVGVNKMD 509
K +I+ NK+D
Sbjct: 174 KDIIIIQNKID 184
>At5g39900.1 68418.m04839 GTP-binding protein LepA, putative
GTP-binding protein GUF1 - Saccharomyces cerevisiae,
PIR:S50374
Length = 661
Score = 34.3 bits (75), Expect = 0.091
Identities = 28/93 (30%), Positives = 42/93 (45%)
Frame = +3
Query: 282 ETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLA 461
E S Y + +ID PGH DF + S A+L+V A G QT + LA
Sbjct: 131 EASGYLLNLIDTPGHVDFSYEVSRSLSACQGALLVVDAAQG-------VQAQTVANFYLA 183
Query: 462 FTLGVKQLIVGVNKMDPLNHHTVSPDLRKSRRK 560
F + ++ +NK+D T P+ K++ K
Sbjct: 184 FEANL-TIVPVINKID---QPTADPERVKAQLK 212
Score = 27.9 bits (59), Expect = 7.9
Identities = 16/40 (40%), Positives = 19/40 (47%)
Frame = +1
Query: 31 D*PKMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDK 150
D K EK N +I H+D GKST L+ G I K
Sbjct: 57 DLTKFPSEKIR-NFSIIAHIDHGKSTLADRLMELTGTIKK 95
>At1g06220.2 68414.m00656 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 33.9 bits (74), Expect = 0.12
Identities = 18/49 (36%), Positives = 23/49 (46%)
Frame = +3
Query: 258 IDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 404
+ + L + Y I+D PGH +F M AD AVLIV A G
Sbjct: 197 MSLVLEDSRSKSYLCNIMDTPGHVNFSDEMTASLRLADGAVLIVDAAEG 245
>At1g06220.1 68414.m00655 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 33.9 bits (74), Expect = 0.12
Identities = 18/49 (36%), Positives = 23/49 (46%)
Frame = +3
Query: 258 IDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 404
+ + L + Y I+D PGH +F M AD AVLIV A G
Sbjct: 197 MSLVLEDSRSKSYLCNIMDTPGHVNFSDEMTASLRLADGAVLIVDAAEG 245
>At4g11160.1 68417.m01808 translation initiation factor IF-2,
mitochondrial, putative similar to SP|P46198|IF2M_BOVIN
Translation initiation factor IF-2, mitochondrial
precursor (IF-2Mt) (IF-2(Mt)) {Bos taurus}
Length = 743
Score = 32.7 bits (71), Expect = 0.28
Identities = 14/35 (40%), Positives = 19/35 (54%)
Frame = +3
Query: 300 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 404
+T +D PGH F + G + D VL+VAA G
Sbjct: 270 ITFLDTPGHAAFSEMRARGAAVTDIVVLVVAADDG 304
>At3g22980.1 68416.m02898 elongation factor Tu family protein
similar to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 32.7 bits (71), Expect = 0.28
Identities = 13/25 (52%), Positives = 16/25 (64%)
Frame = +1
Query: 67 NIVVIGHVDSGKSTTTGHLIYKCGG 141
NI ++ HVD GK+T HLI GG
Sbjct: 11 NICILAHVDHGKTTLADHLIASSGG 35
Score = 32.7 bits (71), Expect = 0.28
Identities = 14/37 (37%), Positives = 22/37 (59%)
Frame = +3
Query: 294 YYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 404
Y + +ID+PGH DF + T +D A+++V A G
Sbjct: 74 YSLNLIDSPGHMDFCSEVSTAARLSDGALVLVDAVEG 110
>At1g62750.1 68414.m07082 elongation factor Tu family protein
similar to elongation factor G SP:P34811 [Glycine max
(Soybean)]
Length = 783
Score = 32.7 bits (71), Expect = 0.28
Identities = 12/28 (42%), Positives = 20/28 (71%)
Frame = +1
Query: 55 KTHINIVVIGHVDSGKSTTTGHLIYKCG 138
K + NI ++ H+D+GK+TTT ++Y G
Sbjct: 94 KDYRNIGIMAHIDAGKTTTTERILYYTG 121
>At2g45030.1 68415.m05606 mitochondrial elongation factor, putative
similar to SP|P25039 Elongation factor G 1,
mitochondrial precursor (mEF-G-1) {Saccharomyces
cerevisiae}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03764: Elongation factor
G domain IV, PF00679: Elongation factor G C-terminus
Length = 754
Score = 31.5 bits (68), Expect = 0.64
Identities = 17/52 (32%), Positives = 25/52 (48%)
Frame = +3
Query: 294 YYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREH 449
Y V IID PGH DF + D A+L++ + G I+ + Q R +
Sbjct: 133 YKVNIIDTPGHVDFTIEVERALRVLDGAILVLCSVGGVQSQSITVDRQMRRY 184
Score = 27.9 bits (59), Expect = 7.9
Identities = 11/26 (42%), Positives = 17/26 (65%)
Frame = +1
Query: 67 NIVVIGHVDSGKSTTTGHLIYKCGGI 144
NI + H+DSGK+T T +++ G I
Sbjct: 67 NIGISAHIDSGKTTLTERVLFYTGRI 92
>At1g45332.1 68414.m05195 mitochondrial elongation factor, putative
similar to mitochondrial elongation factor GI:3917 from
[Saccharomyces cerevisiae]
Length = 754
Score = 31.5 bits (68), Expect = 0.64
Identities = 17/52 (32%), Positives = 25/52 (48%)
Frame = +3
Query: 294 YYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREH 449
Y V IID PGH DF + D A+L++ + G I+ + Q R +
Sbjct: 133 YKVNIIDTPGHVDFTIEVERALRVLDGAILVLCSVGGVQSQSITVDRQMRRY 184
Score = 27.9 bits (59), Expect = 7.9
Identities = 11/26 (42%), Positives = 17/26 (65%)
Frame = +1
Query: 67 NIVVIGHVDSGKSTTTGHLIYKCGGI 144
NI + H+DSGK+T T +++ G I
Sbjct: 67 NIGISAHIDSGKTTLTERVLFYTGRI 92
>At1g76720.1 68414.m08929 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03144: Elongation factor
Tu domain 2
Length = 1201
Score = 31.1 bits (67), Expect = 0.85
Identities = 22/70 (31%), Positives = 35/70 (50%)
Frame = +3
Query: 306 IIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQL 485
+ID PGH F G+S D A+L+V + + G+ QT E +L + +
Sbjct: 705 VIDTPGHESFTNLRSRGSSLCDLAILVV-----DIKHGLEP--QTIE-SLNLLRMRNTEF 756
Query: 486 IVGVNKMDPL 515
I+ +NK+D L
Sbjct: 757 IIALNKVDRL 766
>At1g21160.1 68414.m02646 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03144: Elongation factor
Tu domain 2
Length = 1088
Score = 31.1 bits (67), Expect = 0.85
Identities = 23/72 (31%), Positives = 34/72 (47%)
Frame = +3
Query: 300 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVK 479
+ +ID PGH F G++ D A+L+V + G+ QT E L VK
Sbjct: 558 ILVIDTPGHESFTNLRSRGSNLCDLAILVV-----DIMRGLEP--QTIESLNLLRRRNVK 610
Query: 480 QLIVGVNKMDPL 515
I+ +NK+D L
Sbjct: 611 -FIIALNKVDRL 621
>At5g08650.1 68418.m01029 GTP-binding protein LepA, putative
Length = 681
Score = 30.7 bits (66), Expect = 1.1
Identities = 13/42 (30%), Positives = 23/42 (54%)
Frame = +3
Query: 279 FETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 404
+E + + + +ID PGH DF + + + A+L+V A G
Sbjct: 147 YEDTPFCLNLIDTPGHVDFSYEVSRSLAACEGALLVVDASQG 188
Score = 28.3 bits (60), Expect = 6.0
Identities = 11/33 (33%), Positives = 18/33 (54%)
Frame = +1
Query: 67 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEK 165
N +I H+D GKST L+ G + R +++
Sbjct: 88 NFSIIAHIDHGKSTLADKLLQVTGTVQNRDMKE 120
>At1g76825.1 68414.m08940 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profile PF00009: Elongation
factor Tu GTP binding domain
Length = 630
Score = 30.3 bits (65), Expect = 1.5
Identities = 22/70 (31%), Positives = 34/70 (48%)
Frame = +3
Query: 306 IIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQL 485
+ID PGH F G+S D A+L+V + G+ QT E +L + +
Sbjct: 113 VIDTPGHESFTNLRSRGSSLCDLAILVV-----DITHGLQP--QTIE-SLNLLRMRNTEF 164
Query: 486 IVGVNKMDPL 515
I+ +NK+D L
Sbjct: 165 IIALNKVDRL 174
>At1g76810.1 68414.m08938 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to IF2
protein [Drosophila melanogaster] GI:7108770; contains
Pfam profile PF03144: Elongation factor Tu domain 2
Length = 1294
Score = 30.3 bits (65), Expect = 1.5
Identities = 23/70 (32%), Positives = 34/70 (48%)
Frame = +3
Query: 306 IIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQL 485
+ID PGH F G+S D A+L+V + G+ QT E +L + +
Sbjct: 772 VIDTPGHESFTNLRSRGSSLCDLAILVV-----DIMHGLEP--QTIE-SLNLLRMRNTEF 823
Query: 486 IVGVNKMDPL 515
IV +NK+D L
Sbjct: 824 IVALNKVDRL 833
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 29.5 bits (63), Expect = 2.6
Identities = 11/34 (32%), Positives = 20/34 (58%)
Frame = +3
Query: 288 SKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 389
++Y + +ID+PGH DF + D A+++V
Sbjct: 96 NEYLINLIDSPGHVDFSSEVTAALRITDGALVVV 129
Score = 29.1 bits (62), Expect = 3.4
Identities = 13/24 (54%), Positives = 15/24 (62%)
Frame = +1
Query: 67 NIVVIGHVDSGKSTTTGHLIYKCG 138
N+ VI HVD GKST T L+ G
Sbjct: 21 NMSVIAHVDHGKSTLTDSLVAAAG 44
>At5g44785.2 68418.m05490 expressed protein contains Pfam PF05329:
Protein of unknown function (DUF731)
Length = 442
Score = 28.7 bits (61), Expect = 4.5
Identities = 15/40 (37%), Positives = 22/40 (55%)
Frame = +2
Query: 503 NGSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPIS 622
N +TE + P+ E K E+S++I IG+ V F P S
Sbjct: 61 NVATEKESTPPKKIEYKPEISNWINLIGFVEQPVQFGPCS 100
>At5g44785.1 68418.m05489 expressed protein contains Pfam PF05329:
Protein of unknown function (DUF731)
Length = 440
Score = 28.7 bits (61), Expect = 4.5
Identities = 15/40 (37%), Positives = 22/40 (55%)
Frame = +2
Query: 503 NGSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPIS 622
N +TE + P+ E K E+S++I IG+ V F P S
Sbjct: 61 NVATEKESTPPKKIEYKPEISNWINLIGFVEQPVQFGPCS 100
>At1g62660.1 68414.m07071 beta-fructosidase (BFRUCT3) /
beta-fructofuranosidase / invertase, vacuolar identical
to beta-fructosidase GB:CAA67560 GI:1429209 [Arabidopsis
thaliana]; supported by full-length cDNA GI:14517549;
identical to cDNA Beta-fructosidase GI:3115854
Length = 648
Score = 28.7 bits (61), Expect = 4.5
Identities = 17/54 (31%), Positives = 24/54 (44%), Gaps = 1/54 (1%)
Frame = -1
Query: 539 IWAHCMVVQWIHFVYSYDELFDTEGESEQGMLTG-LTVLRDTSFEFTGTGSYDE 381
+W H + IH++Y + + G+ TG T L D S TGS DE
Sbjct: 148 VWGHAVSKDLIHWLYLPIAMVPDQWYDANGVWTGSATFLDDGSIVMLYTGSTDE 201
>At3g20440.1 68416.m02588 glycoside hydrolase family 13 protein
similar to 1,4-alpha-glucan branching enzyme [Solanum
tuberosum] GI:1621012, 1,4-alpha-glucan branching enzyme
(EC 2.4.1.18) from [Homo sapiens] SP|Q04446, {Solanum
tuberosum} SP|P30924; contains Pfam profiles: PF00128
Alpha amylase catalytic domain, PF02922 Isoamylase
N-terminal domain
Length = 777
Score = 28.3 bits (60), Expect = 6.0
Identities = 14/33 (42%), Positives = 20/33 (60%), Gaps = 3/33 (9%)
Frame = +2
Query: 527 SEPR---FEEIKKEVSSYIKKIGYNPAAVAFVP 616
SEP+ FEE K+V ++K+ GYN + VP
Sbjct: 257 SEPKVSTFEEFTKKVLPHVKRAGYNAIQLIGVP 289
>At1g22730.1 68414.m02840 MA3 domain-containing protein contains
Pfam profile PF02847: MA3 domain; low similarity to
programmed cell death 4 protein [Gallus gallus]
GI:12958564
Length = 693
Score = 28.3 bits (60), Expect = 6.0
Identities = 13/33 (39%), Positives = 19/33 (57%)
Frame = +3
Query: 468 LGVKQLIVGVNKMDPLNHHTVSPDLRKSRRKYP 566
L + QL + + +DPL ++ DL KSRR P
Sbjct: 14 LCISQLKISSSSLDPLPQANMAEDLTKSRRHSP 46
>At1g17220.1 68414.m02098 translation initiation factor IF-2,
chloroplast, putative similar to SP|P57997|IF2C_PHAVU
Translation initiation factor IF-2, chloroplast
precursor (PvIF2cp) {Phaseolus vulgaris}
Length = 1026
Score = 28.3 bits (60), Expect = 6.0
Identities = 19/67 (28%), Positives = 29/67 (43%)
Frame = +3
Query: 309 IDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLI 488
+D PGH F G D A+++VAA G QT E A+ ++
Sbjct: 557 LDTPGHEAFGAMRARGARVTDIAIIVVAADDG-------IRPQTNE-AIAHAKAAAVPIV 608
Query: 489 VGVNKMD 509
+ +NK+D
Sbjct: 609 IAINKID 615
>At2g28000.1 68415.m03393 RuBisCO subunit binding-protein alpha
subunit, chloroplast / 60 kDa chaperonin alpha subunit /
CPN-60 alpha identical to SWISS-PROT:P21238- RuBisCO
subunit binding-protein alpha subunit, chloroplast
precursor (60 kDa chaperonin alpha subunit, CPN-60
alpha) [Arabidopsis thaliana]
Length = 586
Score = 27.9 bits (59), Expect = 7.9
Identities = 16/58 (27%), Positives = 29/58 (50%)
Frame = -1
Query: 392 SYDEHSAISLRGSCDHVLDEISVSRSINDGNIVLASFELPESNIDCIPSSRSAFSLSN 219
++D+HS +L+ D + D + ++ N+VL F P+ D + +R A L N
Sbjct: 52 AFDQHSRAALQAGIDKLADCVGLTLGPRGRNVVLDEFGSPKVVNDGVTIAR-AIELPN 108
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,763,629
Number of Sequences: 28952
Number of extensions: 347666
Number of successful extensions: 1046
Number of sequences better than 10.0: 33
Number of HSP's better than 10.0 without gapping: 982
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1039
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1721869952
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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