Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ps4M0065.Seq
(620 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g77440.1 68414.m09018 20S proteasome beta subunit C (PBC2) id... 89 2e-18
At1g21720.1 68414.m02719 20S proteasome beta subunit C1 (PBC1) (... 86 2e-17
At3g60820.1 68416.m06804 20S proteasome beta subunit F1 (PBF1) 36 0.022
At4g39770.1 68417.m05632 trehalose-6-phosphate phosphatase, puta... 29 1.9
At5g25520.2 68418.m03037 transcription elongation factor-related... 29 3.3
At4g05160.1 68417.m00775 4-coumarate--CoA ligase, putative / 4-c... 29 3.3
At5g63380.1 68418.m07955 4-coumarate--CoA ligase family protein ... 28 5.7
At3g54000.3 68416.m05969 expressed protein 28 5.7
At3g54000.2 68416.m05968 expressed protein 28 5.7
At3g54000.1 68416.m05970 expressed protein 28 5.7
>At1g77440.1 68414.m09018 20S proteasome beta subunit C (PBC2)
identical to residues 14-204 of 20S proteasome beta
subunit PBC2 GB:AAC32069 [Arabidopsis thaliana]
Length = 204
Score = 89.0 bits (211), Expect = 2e-18
Identities = 54/146 (36%), Positives = 79/146 (54%)
Frame = +3
Query: 78 MSILAYNGGAVVAMKGQDCVAIATDKRFGIQAQTVSTQLPKSIPXGTHXVXRPSRXRX*T 257
MSI YNG AVVAM G++C AIA+D+R G+Q QT++T + H S
Sbjct: 1 MSIFEYNGSAVVAMVGKNCFAIASDRRLGVQLQTIATDFQRISKIHDHLFIGLSGLATDV 60
Query: 258 QTVXQXXXFRXNLYXLKEXXLXRLKXFSXMLSTLLYDRRFGPYLWNLS*LA*IPYXNPTL 437
QT+ Q FR LY L+E + + F+ ++S +LY++RFGP+L +A + N
Sbjct: 61 QTLYQRLVFRHKLYQLREERDMKPETFASLVSAILYEKRFGPFLCQ-PVIAGLGDDNKP- 118
Query: 438 MVCXMA*LVAQMTLKILWVSGPCSKS 515
+C M + A+ K VSG S+S
Sbjct: 119 FICTMDSIGAKELAKDFVVSGTASES 144
Score = 35.5 bits (78), Expect = 0.029
Identities = 22/56 (39%), Positives = 27/56 (48%)
Frame = +2
Query: 374 FRPLLMEPVIAGLDPLX*PNPYGLXHGLIGCPNDPEDFVGIGPLFEKLYGMCXALW 541
F P L +PVIAGL P+ IG +DFV G E LYG C A++
Sbjct: 100 FGPFLCQPVIAGLGDDN--KPFICTMDSIGAKELAKDFVVSGTASESLYGACEAMF 153
>At1g21720.1 68414.m02719 20S proteasome beta subunit C1 (PBC1)
(PRCT) almost identical to GB:AAC32069 from [Arabidopsis
thaliana], EST gb|T76747 comes from this gene; identical
to cDNA proteasome subunit prct GI:2511567
Length = 204
Score = 85.8 bits (203), Expect = 2e-17
Identities = 43/104 (41%), Positives = 61/104 (58%)
Frame = +3
Query: 78 MSILAYNGGAVVAMKGQDCVAIATDKRFGIQAQTVSTQLPKSIPXGTHXVXRPSRXRX*T 257
MSI YNG AVVAM G++C AIA+D+R G+Q QT++T + S
Sbjct: 1 MSIFEYNGSAVVAMVGKNCFAIASDRRLGVQLQTIATDFQRISKIHDRVFIGLSGLATDV 60
Query: 258 QTVXQXXXFRXNLYXLKEXXLXRLKXFSXMLSTLLYDRRFGPYL 389
QT+ Q FR LY L+E + + F+ ++S +LY++RFGPYL
Sbjct: 61 QTLYQRLVFRHKLYQLREERDMKPETFASLVSAILYEKRFGPYL 104
Score = 35.5 bits (78), Expect = 0.029
Identities = 22/56 (39%), Positives = 27/56 (48%)
Frame = +2
Query: 374 FRPLLMEPVIAGLDPLX*PNPYGLXHGLIGCPNDPEDFVGIGPLFEKLYGMCXALW 541
F P L +PVIAGL P+ IG +DFV G E LYG C A++
Sbjct: 100 FGPYLCQPVIAGLGDDD--KPFICTMDSIGAKELAKDFVVSGTASESLYGACEAMY 153
>At3g60820.1 68416.m06804 20S proteasome beta subunit F1 (PBF1)
Length = 223
Score = 35.9 bits (79), Expect = 0.022
Identities = 27/104 (25%), Positives = 38/104 (36%)
Frame = +3
Query: 75 NMSILAYNGGAVVAMKGQDCVAIATDKRFGIQAQTVSTQLPKSIPXGTHXVXRPSRXRX* 254
N S NGG VA+ G D IA D R +S K V S +
Sbjct: 7 NWSPYDNNGGTCVAIAGSDYCVIAADTRMSTGYSILSRDYSKIHKLADRAVLSSSGFQAD 66
Query: 255 TQTVXQXXXFRXNLYXLKEXXLXRLKXFSXMLSTLLYDRRFGPY 386
+ + + R +Y + + +LS LY +RF PY
Sbjct: 67 VKALQKVLKSRHLIYQHQHNKQMSCPAMAQLLSNTLYFKRFFPY 110
>At4g39770.1 68417.m05632 trehalose-6-phosphate phosphatase,
putative similar to trehalose-6-phosphate phosphatase
(AtTPPB) [Arabidopsis thaliana] GI:2944180; contains
Pfam profile PF02358: Trehalose-phosphatase
Length = 349
Score = 29.5 bits (63), Expect = 1.9
Identities = 11/26 (42%), Positives = 15/26 (57%)
Frame = -2
Query: 202 LLGSWVDTV*ACIPNRLSVAMATQSW 125
L+ SWVD++ AC P L + SW
Sbjct: 43 LINSWVDSMRACSPTHLKSLLKQSSW 68
>At5g25520.2 68418.m03037 transcription elongation factor-related
contains weak similarity to transcription elongation
factors
Length = 997
Score = 28.7 bits (61), Expect = 3.3
Identities = 12/23 (52%), Positives = 15/23 (65%), Gaps = 1/23 (4%)
Frame = +2
Query: 449 HGLIGCPNDPEDF-VGIGPLFEK 514
HGL+GC +D ED G GP+ K
Sbjct: 853 HGLVGCDDDDEDMPPGFGPVAAK 875
>At4g05160.1 68417.m00775 4-coumarate--CoA ligase, putative /
4-coumaroyl-CoA synthase, putative similar to 4CL2
[gi:12229665] from Arabidopsis thaliana, 4CL1
[gi:12229631] from Nicotiana tabacum; contains Pfam
AMP-binding enzyme domain PF00501; acyl-activating
enzyme superfamily; identical to cDNA 4-coumarate-CoA
ligase-like protein (At4g05160) GI:29893226
Length = 544
Score = 28.7 bits (61), Expect = 3.3
Identities = 15/32 (46%), Positives = 20/32 (62%), Gaps = 2/32 (6%)
Frame = -1
Query: 497 RYPQNLQGHLGN-QSSHXT-DHKGWVXIGDLG 408
R P ++G+L N Q++ T D K WV GDLG
Sbjct: 395 RGPNMMKGYLNNPQATKETIDKKSWVHTGDLG 426
>At5g63380.1 68418.m07955 4-coumarate--CoA ligase family protein /
4-coumaroyl-CoA synthase family protein similar to 4CL2
[gi:12229665] from Arabidopsis thaliana, 4CL1
[gi:12229631] from Nicotiana tabacum; contains Pfam
AMP-binding enzyme domain PF00501
Length = 562
Score = 27.9 bits (59), Expect = 5.7
Identities = 12/31 (38%), Positives = 20/31 (64%), Gaps = 2/31 (6%)
Frame = -1
Query: 497 RYPQNLQGHLGNQ--SSHXTDHKGWVXIGDL 411
R P ++G++GN+ S+ D +GW+ GDL
Sbjct: 409 RGPVIMKGYVGNEKASAETVDKEGWLKTGDL 439
>At3g54000.3 68416.m05969 expressed protein
Length = 301
Score = 27.9 bits (59), Expect = 5.7
Identities = 18/55 (32%), Positives = 24/55 (43%), Gaps = 1/55 (1%)
Frame = -3
Query: 507 NRGPIPTKSSGSFGQ-PIKPXYRP*GLGXHRGSRPAMTGSISKGRNVGHTIRLTT 346
N GP+ SS Q P + R +G H G R + + R+V HT R T
Sbjct: 226 NVGPVDLSSSAWSNQFPRRDVMRAVFIGDHTGKRGSTGTGVFLPRSVNHTSRTET 280
>At3g54000.2 68416.m05968 expressed protein
Length = 301
Score = 27.9 bits (59), Expect = 5.7
Identities = 18/55 (32%), Positives = 24/55 (43%), Gaps = 1/55 (1%)
Frame = -3
Query: 507 NRGPIPTKSSGSFGQ-PIKPXYRP*GLGXHRGSRPAMTGSISKGRNVGHTIRLTT 346
N GP+ SS Q P + R +G H G R + + R+V HT R T
Sbjct: 226 NVGPVDLSSSAWSNQFPRRDVMRAVFIGDHTGKRGSTGTGVFLPRSVNHTSRTET 280
>At3g54000.1 68416.m05970 expressed protein
Length = 352
Score = 27.9 bits (59), Expect = 5.7
Identities = 18/55 (32%), Positives = 24/55 (43%), Gaps = 1/55 (1%)
Frame = -3
Query: 507 NRGPIPTKSSGSFGQ-PIKPXYRP*GLGXHRGSRPAMTGSISKGRNVGHTIRLTT 346
N GP+ SS Q P + R +G H G R + + R+V HT R T
Sbjct: 226 NVGPVDLSSSAWSNQFPRRDVMRAVFIGDHTGKRGSTGTGVFLPRSVNHTSRTET 280
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,347,940
Number of Sequences: 28952
Number of extensions: 231463
Number of successful extensions: 433
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 421
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 429
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1255974912
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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