Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= pg--0027.Seq
(697 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g03340.1 68418.m00286 cell division cycle protein 48, putativ... 141 5e-34
At3g09840.1 68416.m01174 cell division cycle protein 48 (CDC48A)... 141 5e-34
At3g53230.1 68416.m05865 cell division cycle protein 48, putativ... 136 2e-32
At3g26630.1 68416.m03328 pentatricopeptide (PPR) repeat-containi... 33 0.18
At4g13885.1 68417.m02151 3'-5' exonuclease-related contains weak... 31 0.96
At2g14120.2 68415.m01573 dynamin-like protein 2b (ADL2b) identic... 29 3.9
At2g14120.1 68415.m01572 dynamin-like protein 2b (ADL2b) identic... 29 3.9
At1g30750.1 68414.m03758 expressed protein 29 3.9
At5g10520.1 68418.m01218 protein kinase family protein contains ... 27 9.0
At2g22810.1 68415.m02707 1-aminocyclopropane-1-carboxylate synth... 27 9.0
At1g49400.1 68414.m05537 ribosomal protein S17 family protein si... 27 9.0
>At5g03340.1 68418.m00286 cell division cycle protein 48, putative /
CDC48, putative very strong similarity to SP|P54609 Cell
division cycle protein 48 homolog {Arabidopsis
thaliana}; contains Pfam profiles PF00004: ATPase AAA
family, PF02359: Cell division protein 48 (CDC48)
N-terminal domain; supporting cDNA
gi|26449351|dbj|AK117125.1|
Length = 810
Score = 141 bits (341), Expect = 5e-34
Identities = 59/85 (69%), Positives = 73/85 (85%)
Frame = +3
Query: 255 DDNCPDEKIRMNHVVRNNLRVRLSDVVSIAPCPSVKYGKRVHILPIDDSVEGLTGNLFEV 434
D+ C + KIRMN VVR+NLRVRL DV+S+ CP VKYGKRVHILP+DD+VEG+TGNLF+
Sbjct: 78 DETCEEPKIRMNKVVRSNLRVRLGDVISVHQCPDVKYGKRVHILPVDDTVEGVTGNLFDA 137
Query: 435 YLKPYFMEAYRPIHRDDTFMVRGGI 509
YLKPYF+EAYRP+ + D F+VRGG+
Sbjct: 138 YLKPYFLEAYRPVRKGDLFLVRGGM 162
Score = 101 bits (241), Expect = 6e-22
Identities = 45/70 (64%), Positives = 59/70 (84%)
Frame = +1
Query: 46 ADNKSPDDLSTAILRRKDRPNRLIVEEAVSDDNSVVALSQAKMEQLQLFRGDTVLLKGKR 225
+D+K+ D STAIL RK PNRL+V+EA++DDNSVV+L ME+LQLFRGDT+L+KGK+
Sbjct: 8 SDSKTKKDFSTAILERKKSPNRLVVDEAINDDNSVVSLHPTTMEKLQLFRGDTILIKGKK 67
Query: 226 RKETVCIVLS 255
RK+TVCI L+
Sbjct: 68 RKDTVCIALA 77
Score = 85.4 bits (202), Expect = 3e-17
Identities = 36/54 (66%), Positives = 46/54 (85%)
Frame = +2
Query: 503 GHRAVEFKVVETDPSPFCIVAPDTVIHCDGEPIKREEEEEALNAVGYDDIGGCR 664
G R+VEFKV+ETDP+ +C+VAPDT I C+GEP+KRE+EE L+ VGYDD+GG R
Sbjct: 161 GMRSVEFKVIETDPAEYCVVAPDTEIFCEGEPVKREDEER-LDEVGYDDVGGVR 213
>At3g09840.1 68416.m01174 cell division cycle protein 48 (CDC48A)
(CDC48) identical to SP|P54609 Cell division cycle
protein 48 homolog {Arabidopsis thaliana}
Length = 809
Score = 141 bits (341), Expect = 5e-34
Identities = 59/85 (69%), Positives = 73/85 (85%)
Frame = +3
Query: 255 DDNCPDEKIRMNHVVRNNLRVRLSDVVSIAPCPSVKYGKRVHILPIDDSVEGLTGNLFEV 434
D+ C + KIRMN VVR+NLRVRL DV+S+ CP VKYGKRVHILP+DD+VEG+TGNLF+
Sbjct: 78 DETCEEPKIRMNKVVRSNLRVRLGDVISVHQCPDVKYGKRVHILPVDDTVEGVTGNLFDA 137
Query: 435 YLKPYFMEAYRPIHRDDTFMVRGGI 509
YLKPYF+EAYRP+ + D F+VRGG+
Sbjct: 138 YLKPYFLEAYRPVRKGDLFLVRGGM 162
Score = 103 bits (248), Expect = 9e-23
Identities = 47/70 (67%), Positives = 60/70 (85%)
Frame = +1
Query: 46 ADNKSPDDLSTAILRRKDRPNRLIVEEAVSDDNSVVALSQAKMEQLQLFRGDTVLLKGKR 225
+D+KS D STAIL RK PNRL+V+EA++DDNSVV+L A ME+LQLFRGDT+L+KGK+
Sbjct: 8 SDSKSKKDFSTAILERKKSPNRLVVDEAINDDNSVVSLHPATMEKLQLFRGDTILIKGKK 67
Query: 226 RKETVCIVLS 255
RK+TVCI L+
Sbjct: 68 RKDTVCIALA 77
Score = 85.0 bits (201), Expect = 4e-17
Identities = 36/54 (66%), Positives = 46/54 (85%)
Frame = +2
Query: 503 GHRAVEFKVVETDPSPFCIVAPDTVIHCDGEPIKREEEEEALNAVGYDDIGGCR 664
G R+VEFKV+ETDP+ +C+VAPDT I C+GEP+KRE+EE L+ VGYDD+GG R
Sbjct: 161 GMRSVEFKVIETDPAEYCVVAPDTEIFCEGEPVKREDEER-LDDVGYDDVGGVR 213
>At3g53230.1 68416.m05865 cell division cycle protein 48, putative /
CDC48, putative very strong similarity to SP|P54609 Cell
division cycle protein 48 homolog {Arabidopsis
thaliana}; contains Pfam profiles PF00004: ATPase AAA
family, PF02359: Cell division protein 48 (CDC48)
N-terminal domain
Length = 815
Score = 136 bits (328), Expect = 2e-32
Identities = 55/85 (64%), Positives = 72/85 (84%)
Frame = +3
Query: 255 DDNCPDEKIRMNHVVRNNLRVRLSDVVSIAPCPSVKYGKRVHILPIDDSVEGLTGNLFEV 434
D+ C + KIRMN VVR+NLRVRL DV+S+ CP VKYG RVHILP+DD++EG++GN+F+
Sbjct: 79 DETCDEPKIRMNKVVRSNLRVRLGDVISVHQCPDVKYGNRVHILPLDDTIEGVSGNIFDA 138
Query: 435 YLKPYFMEAYRPIHRDDTFMVRGGI 509
YLKPYF+EAYRP+ + D F+VRGG+
Sbjct: 139 YLKPYFLEAYRPVRKGDLFLVRGGM 163
Score = 91.5 bits (217), Expect = 5e-19
Identities = 43/71 (60%), Positives = 58/71 (81%), Gaps = 1/71 (1%)
Frame = +1
Query: 46 ADNK-SPDDLSTAILRRKDRPNRLIVEEAVSDDNSVVALSQAKMEQLQLFRGDTVLLKGK 222
+D+K + D STAIL +K NRL+V+EA++DDNSVV+L ME+LQLFRGDT+L+KGK
Sbjct: 8 SDSKGTKKDFSTAILEKKKAANRLVVDEAINDDNSVVSLHPDTMEKLQLFRGDTILIKGK 67
Query: 223 RRKETVCIVLS 255
+RK+TVCI L+
Sbjct: 68 KRKDTVCIALA 78
Score = 85.4 bits (202), Expect = 3e-17
Identities = 36/54 (66%), Positives = 46/54 (85%)
Frame = +2
Query: 503 GHRAVEFKVVETDPSPFCIVAPDTVIHCDGEPIKREEEEEALNAVGYDDIGGCR 664
G R++EFKV+ETDP+ +C+VAPDT I C+GEPIKRE+EE L+ VGYDD+GG R
Sbjct: 162 GMRSIEFKVIETDPAEYCVVAPDTEIFCEGEPIKREDEER-LDEVGYDDVGGVR 214
>At3g26630.1 68416.m03328 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile: PF01535 PPR repeat
Length = 455
Score = 33.1 bits (72), Expect = 0.18
Identities = 16/54 (29%), Positives = 29/54 (53%), Gaps = 1/54 (1%)
Frame = -3
Query: 353 RTRSDGYHIRKTHTKVVSHNMVHPNFLIRAII-VESTMQTVSLRRLPLSKTVSP 195
RT S+ +++ HTK++ HN+ + L+R +I V S+ L ++ SP
Sbjct: 28 RTCSNFSQLKQIHTKIIKHNLTNDQLLVRQLISVSSSFGETQYASLVFNQLQSP 81
>At4g13885.1 68417.m02151 3'-5' exonuclease-related contains weak
similarity to Pfam domain PF01612: 3'-5' exonuclease
Length = 263
Score = 30.7 bits (66), Expect = 0.96
Identities = 13/23 (56%), Positives = 18/23 (78%)
Frame = -2
Query: 198 TTEELKLLHFGL*KCHD*VVIAD 130
TTEELK+ H+ L KC D +V+A+
Sbjct: 3 TTEELKISHYKLYKCFDFLVVAN 25
>At2g14120.2 68415.m01573 dynamin-like protein 2b (ADL2b) identical
to dynamin like protein 2b (ADL2b) [Arabidopsis
thaliana] GI:19032339
Length = 780
Score = 28.7 bits (61), Expect = 3.9
Identities = 13/27 (48%), Positives = 15/27 (55%)
Frame = -3
Query: 296 NMVHPNFLIRAIIVESTMQTVSLRRLP 216
N HPNF+ VE MQTV R+P
Sbjct: 508 NTSHPNFIGGTKAVEQAMQTVKSSRIP 534
>At2g14120.1 68415.m01572 dynamin-like protein 2b (ADL2b) identical
to dynamin like protein 2b (ADL2b) [Arabidopsis
thaliana] GI:19032339
Length = 780
Score = 28.7 bits (61), Expect = 3.9
Identities = 13/27 (48%), Positives = 15/27 (55%)
Frame = -3
Query: 296 NMVHPNFLIRAIIVESTMQTVSLRRLP 216
N HPNF+ VE MQTV R+P
Sbjct: 508 NTSHPNFIGGTKAVEQAMQTVKSSRIP 534
>At1g30750.1 68414.m03758 expressed protein
Length = 212
Score = 28.7 bits (61), Expect = 3.9
Identities = 13/55 (23%), Positives = 26/55 (47%)
Frame = +2
Query: 83 SSVARTDPTVSLSKKQSAMTTQSWHFHRPKWSNFNSSVVTQSCSRANAARKPFAS 247
SS +P + S++ F++P + FN + + S S ++A +PF +
Sbjct: 115 SSTGLNNPILGQPSTSSSLNQPLTGFNQPSSTGFNQPISSSSSSSSSAQNQPFTN 169
>At5g10520.1 68418.m01218 protein kinase family protein contains
protein kinase domain, INTERPRO:IPR000719
Length = 467
Score = 27.5 bits (58), Expect = 9.0
Identities = 14/49 (28%), Positives = 25/49 (51%), Gaps = 1/49 (2%)
Frame = +2
Query: 41 KWQIIKALMIYR-PRSSVARTDPTVSLSKKQSAMTTQSWHFHRPKWSNF 184
++ +I L Y R ++ R P L+ +SA T +++ +P W NF
Sbjct: 95 RFSVIPLLASYELTRKNLRRKQP--KLTPSESAFTCEAFFMAKPSWRNF 141
>At2g22810.1 68415.m02707 1-aminocyclopropane-1-carboxylate synthase
4 / ACC synthase 4 (ACS4) identical to gi:940370
[GB:U23481]
Length = 474
Score = 27.5 bits (58), Expect = 9.0
Identities = 14/43 (32%), Positives = 19/43 (44%)
Frame = -2
Query: 345 ERWIPHQKDAHEGCFSQHGSSEFSHQGNYRREHDANGFLAAFA 217
E W+ DA CF + G S F ++ H + F AFA
Sbjct: 56 ESWLAQNTDA--ACFKRDGQSVFRELALFQDYHGLSSFKNAFA 96
>At1g49400.1 68414.m05537 ribosomal protein S17 family protein
similar to 40S ribosomal protein S17 GI:1620985 from
[Nicotiana plumbaginifolia]
Length = 116
Score = 27.5 bits (58), Expect = 9.0
Identities = 18/44 (40%), Positives = 23/44 (52%)
Frame = +2
Query: 20 SNNKF*IKWQIIKALMIYRPRSSVARTDPTVSLSKKQSAMTTQS 151
S NK I +IIK IY P+++ A + S S SA TT S
Sbjct: 64 SKNKHWIVAEIIKKARIYSPKAAAAAVSASASAS---SASTTDS 104
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,656,247
Number of Sequences: 28952
Number of extensions: 336946
Number of successful extensions: 973
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 941
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 970
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1487069504
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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