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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ovS301A12f
         (516 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g65560.1 68414.m07437 allyl alcohol dehydrogenase, putative s...    49   2e-06
At5g17000.1 68418.m01991 NADP-dependent oxidoreductase, putative...    48   5e-06
At5g16990.1 68418.m01990 NADP-dependent oxidoreductase, putative...    45   3e-05
At3g03080.1 68416.m00304 NADP-dependent oxidoreductase, putative...    43   1e-04
At5g16970.1 68418.m01988 NADP-dependent oxidoreductase, putative...    40   0.001
At1g26320.1 68414.m03210 NADP-dependent oxidoreductase, putative...    38   0.003
At5g16960.1 68418.m01987 NADP-dependent oxidoreductase, putative...    35   0.028
At3g08490.1 68416.m00984 hypothetical protein                          33   0.11 
At3g53810.1 68416.m05945 lectin protein kinase, putative similar...    27   9.9  

>At1g65560.1 68414.m07437 allyl alcohol dehydrogenase, putative
           similar to allyl alcohol dehydrogenase from Nicotiana
           tabacum [gi:6692816]; similar to probable NADP-dependent
           oxidoreductase (zeta-crystallin homolog) P1
           [SP|Q39172][gi:886428] and P2 [SP|Q39173][gi:886430],
           Arabidopsis thaliana
          Length = 350

 Score = 49.2 bits (112), Expect = 2e-06
 Identities = 34/125 (27%), Positives = 58/125 (46%), Gaps = 13/125 (10%)
 Frame = +2

Query: 176 NMVIAKKIIVVNPFVGVPKHNDFDI----VXXXXXXXXXXXFLAEAIYFSVDPYQRMKL- 340
           ++V  KK+I+ N   G+P   D ++                FL + +Y S DPY R ++ 
Sbjct: 5   SVVENKKVILKNYVDGIPTETDMEVKLGETIELKAPKGSSCFLVKNLYLSCDPYMRGRMR 64

Query: 341 ---GNVYPCDMIGGQI-----ARVIESKNTEYPVGTLVMGHFGWRTHTITKPENESSVNK 496
              G+  P  + G +I     ARVI+S +T Y  G +V G  GW  +++ +  +   +  
Sbjct: 65  DFHGSYLPPFVPGQRIEGFGLARVIDSDDTNYKPGDIVSGIIGWEEYSLLRSSDNLQLRN 124

Query: 497 TLLDN 511
             LD+
Sbjct: 125 IQLDD 129


>At5g17000.1 68418.m01991 NADP-dependent oxidoreductase, putative
           strong similarity to probable NADP-dependent
           oxidoreductase (zeta-crystallin homolog) P1
           [SP|Q39172][gi:886428] and P2 [SP|Q39173][gi:886430],
           Arabidopsis thaliana
          Length = 345

 Score = 47.6 bits (108), Expect = 5e-06
 Identities = 30/109 (27%), Positives = 49/109 (44%), Gaps = 15/109 (13%)
 Frame = +2

Query: 191 KKIIVVNPFVGVPKHNDFDI---VXXXXXXXXXXXFLAEAIYFSVDPYQRMKLGNVYPCD 361
           K++++ N   G PK +DFD                 L + +Y S DPY R+++G   P  
Sbjct: 6   KQVVLKNYVSGFPKESDFDFKTTTVEFKLPGGSNSVLVKNLYLSCDPYMRIRMGKPDPST 65

Query: 362 ------------MIGGQIARVIESKNTEYPVGTLVMGHFGWRTHTITKP 472
                       + G  ++RVIES + +Y  G L+ G  GW  +++  P
Sbjct: 66  AALAQAYAPGKPIFGYGVSRVIESGHPDYKKGDLLWGIVGWEEYSVITP 114


>At5g16990.1 68418.m01990 NADP-dependent oxidoreductase, putative
           strong similarity to probable NADP-dependent
           oxidoreductase (zeta-crystallin homolog) P1
           [SP|Q39172][gi:886428] and P2 [SP|Q39173][gi:886430],
           Arabidopsis thaliana
          Length = 343

 Score = 44.8 bits (101), Expect = 3e-05
 Identities = 30/112 (26%), Positives = 48/112 (42%), Gaps = 14/112 (12%)
 Frame = +2

Query: 179 MVIAKKIIVVNPFVGVPKHNDFDIVXXXXXXXXXXX---FLAEAIYFSVDPYQRMKLGNV 349
           M   K++I  +   G PK +DF+                 L + +Y S DPY R ++G  
Sbjct: 1   MTTNKQVIFKDHVSGFPKESDFNFTTTTVELRVPEGSKSVLVKNLYLSCDPYMRSRMGKP 60

Query: 350 YPCDMI-----------GGQIARVIESKNTEYPVGTLVMGHFGWRTHTITKP 472
            P   +           G  ++RVIES + +Y  G L+ G  GW  +++  P
Sbjct: 61  DPSSALAQAYAPGKPIYGYGVSRVIESGHPDYKKGDLLWGIVGWEEYSVITP 112


>At3g03080.1 68416.m00304 NADP-dependent oxidoreductase, putative
           similar to probable NADP-dependent oxidoreductase
           (zeta-crystallin homolog)  P2 [SP|Q39173][gi:886430],
           Arabidopsis thaliana; similar to allyl alcohol
           dehydrogenase [Nicotiana tabacum] GI:6692816
          Length = 350

 Score = 43.2 bits (97), Expect = 1e-04
 Identities = 29/116 (25%), Positives = 52/116 (44%), Gaps = 16/116 (13%)
 Frame = +2

Query: 176 NMVIAKKIIVVNPFV-GVPKHNDF---DIVXXXXXXXXXXXFLAEAIYFSVDPYQRMKLG 343
           N  +A K +++  +V G PK +D    D              L + +Y S DPY R+++G
Sbjct: 6   NATVANKQVILRDYVTGFPKESDLIFTDSTIDLKIPEGSKTVLVKNLYLSCDPYMRIRMG 65

Query: 344 NVYPC------------DMIGGQIARVIESKNTEYPVGTLVMGHFGWRTHTITKPE 475
              P              + G  +++VI+S + +Y  G L+ G  GW  +++  P+
Sbjct: 66  KPDPGTAALAPHYIPGEPIYGFSVSKVIDSGHPDYKKGDLLWGLVGWGEYSLITPD 121


>At5g16970.1 68418.m01988 NADP-dependent oxidoreductase, putative
           (P1) identical to probable NADP-dependent oxidoreductase
           P1, zeta-crystallin homolog [SP|Q39172][gi:886428],
           Arabidopsis thaliana; similar to allyl alcohol
           dehydrogenase [Nicotiana tabacum] GI:6692816; contains
           Pfam profile PF00107: oxidoreductase, zinc-binding
           dehydrogenase family
          Length = 345

 Score = 39.9 bits (89), Expect = 0.001
 Identities = 28/114 (24%), Positives = 48/114 (42%), Gaps = 16/114 (14%)
 Frame = +2

Query: 179 MVIAKKIIVVNPFV-GVPKHNDFDIVXXXXXXXXXXX---FLAEAIYFSVDPYQRMKLGN 346
           M    K +++  +V G P  +DFD                 L + +Y S DPY R+++G 
Sbjct: 1   MTATNKQVILKDYVSGFPTESDFDFTTTTVELRVPEGTNSVLVKNLYLSCDPYMRIRMGK 60

Query: 347 VYPC------------DMIGGQIARVIESKNTEYPVGTLVMGHFGWRTHTITKP 472
             P              + G  ++R+IES + +Y  G L+ G   W  +++  P
Sbjct: 61  PDPSTAALAQAYTPGQPIQGYGVSRIIESGHPDYKKGDLLWGIVAWEEYSVITP 114


>At1g26320.1 68414.m03210 NADP-dependent oxidoreductase, putative
           similar to probable NADP-dependent oxidoreductase
           (zeta-crystallin homolog) P1 [SP|Q39172][gi:886428] and
           P2 [SP|Q39173][gi:886430], Arabidopsis thaliana; similar
           to allyl alcohol dehydrogenase GI:9758497 from
           [Arabidopsis thaliana]
          Length = 351

 Score = 38.3 bits (85), Expect = 0.003
 Identities = 29/109 (26%), Positives = 47/109 (43%), Gaps = 15/109 (13%)
 Frame = +2

Query: 182 VIAKKIIVVNPFVGVPKHNDFDIVXXXXXXXXXXX---FLAEAIYFSVDPYQRMKLGNVY 352
           V  K+II  +   G PK +D  I                L + +Y S DPY R+ +G   
Sbjct: 9   VTNKQIIFPDYVTGFPKESDLKITTTTIDLRLPEGSTSVLVKNLYLSCDPYMRICMGKPD 68

Query: 353 PCD------------MIGGQIARVIESKNTEYPVGTLVMGHFGWRTHTI 463
           P              +IG  +++VI+S + +Y  G L+ G  GW  +++
Sbjct: 69  PLSSSLVPPYKTGVPIIGLGVSKVIDSGHPDYKKGDLLWGLVGWEEYSV 117


>At5g16960.1 68418.m01987 NADP-dependent oxidoreductase, putative
           similar to probable NADP-dependent oxidoreductase
           (zeta-crystallin homolog) P1 [SP|Q39172][gi:886428] and
           P2 [SP|Q39173][gi:886430], Arabidopsis thaliana
          Length = 346

 Score = 35.1 bits (77), Expect = 0.028
 Identities = 25/111 (22%), Positives = 48/111 (43%), Gaps = 15/111 (13%)
 Frame = +2

Query: 185 IAKKIIVVNPFVGVPKHNDFDIVXXXXXXXXXXXF---LAEAIYFSVDPYQRMKLGNVYP 355
           I K++++++   G  K +D  I                L + +Y S DPY R ++    P
Sbjct: 5   INKQVVLLDYVTGFAKESDLVITSTTIDLRVLKGSMTALVKNLYLSCDPYMRNRMRKPDP 64

Query: 356 CD------------MIGGQIARVIESKNTEYPVGTLVMGHFGWRTHTITKP 472
                         + G  +++VI+S +++Y  G L+ G  GW  +++  P
Sbjct: 65  LSPATAQSFTPGKPISGFGVSKVIDSGHSDYEEGDLIWGAVGWEEYSVITP 115


>At3g08490.1 68416.m00984 hypothetical protein
          Length = 271

 Score = 33.1 bits (72), Expect = 0.11
 Identities = 12/38 (31%), Positives = 21/38 (55%)
 Frame = -2

Query: 386 LWQFVHQSYHKDIHCLVSFFDKDRH*SILLLLETHFLV 273
           +W  ++  YH  + CL+   DK+RH  + ++ E   LV
Sbjct: 233 VWNIINPKYHHKVECLLLLADKERHSHVTMIREKCRLV 270


>At3g53810.1 68416.m05945 lectin protein kinase, putative similar to
           receptor lectin kinase 3 [Arabidopsis thaliana]
           gi|4100060|gb|AAD00733; contains protein kinase domain,
           Pfam:PF00069; contains legume lectins alpha and beta
           domains, Pfam:PF00138 and Pfam:PF00139
          Length = 677

 Score = 26.6 bits (56), Expect = 9.9
 Identities = 10/21 (47%), Positives = 16/21 (76%)
 Frame = -3

Query: 421 PNRILGIFTFNNSGNLSTNHI 359
           P++ +G+F  +N+GN  TNHI
Sbjct: 120 PSQYIGLFNISNNGN-DTNHI 139


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,318,679
Number of Sequences: 28952
Number of extensions: 189011
Number of successful extensions: 417
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 404
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 412
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 937669760
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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