Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0067.Seq
(568 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g53800.1 68418.m06685 expressed protein 32 0.23
At2g22795.1 68415.m02704 expressed protein 32 0.31
At3g20550.1 68416.m02601 forkhead-associated domain-containing p... 31 0.41
At1g56660.1 68414.m06516 expressed protein 31 0.54
At1g20920.1 68414.m02619 DEAD box RNA helicase, putative similar... 31 0.54
At5g60530.1 68418.m07590 late embryogenesis abundant protein-rel... 30 0.94
At5g55920.1 68418.m06975 nucleolar protein, putative similar to ... 29 2.2
At4g33390.1 68417.m04746 hypothetical protein contains Pfam prof... 29 2.2
At1g31460.1 68414.m03852 expressed protein 29 2.2
At5g48610.1 68418.m06012 expressed protein ; expression supporte... 29 2.9
At3g29075.1 68416.m03637 glycine-rich protein 29 2.9
At1g48970.1 68414.m05489 eukaryotic translation initiation facto... 29 2.9
At3g09620.1 68416.m01141 DEAD/DEAH box helicase, putative simila... 28 3.8
At1g62970.1 68414.m07110 DNAJ heat shock N-terminal domain-conta... 28 5.0
At5g09880.1 68418.m01142 RNA recognition motif (RRM)-containing ... 27 6.6
At3g28110.1 68416.m03508 hypothetical protein 27 6.6
At2g30840.1 68415.m03760 2-oxoglutarate-dependent dioxygenase, p... 27 6.6
At2g16140.1 68415.m01850 expressed protein contains similarity t... 27 8.7
At1g65190.1 68414.m07391 protein kinase family protein contains ... 27 8.7
At1g10320.1 68414.m01162 U2 snRNP auxiliary factor-related simil... 27 8.7
>At5g53800.1 68418.m06685 expressed protein
Length = 351
Score = 32.3 bits (70), Expect = 0.23
Identities = 15/45 (33%), Positives = 25/45 (55%)
Frame = -1
Query: 256 RRNQREVKEKKSKANMRKTTKGKRTTADPPNIQREKERVWKNQKK 122
+R +RE +EK+ K R+ K KR +D ++ KE+ K +K
Sbjct: 136 KRKEREEEEKERKRRRREKDKKKRNKSDKDGDKKRKEKKKKKSEK 180
>At2g22795.1 68415.m02704 expressed protein
Length = 734
Score = 31.9 bits (69), Expect = 0.31
Identities = 30/149 (20%), Positives = 55/149 (36%)
Frame = -1
Query: 568 KKKLEKSKRN*WMSXKSERRLTEXXXXXXXXXXXEGLTLEKEGYKSDYDRNEYEERGSEH 389
K+K E S + ++E + E E +EKE S + E E E
Sbjct: 509 KEKEESSSQEKTEEKETETKDNEESSSQEETKDKENEKIEKEEASSQEESKENETETKEK 568
Query: 388 QEDNDSDXXXXXXXXXXXXXXXXXXXERRTHDKFSIGKNGISAERRNQREVKEKKSKANM 209
+E + + E + + I K E +Q E KEK+++
Sbjct: 569 EESSSQEETKEKENEKIEKEESAPQEETKEKENEKIEKE----ESASQEETKEKETETKE 624
Query: 208 RKTTKGKRTTADPPNIQREKERVWKNQKK 122
++ + + + +KE+V +N+KK
Sbjct: 625 KEESSSNESQENVNTESEKKEQVEENEKK 653
>At3g20550.1 68416.m02601 forkhead-associated domain-containing
protein / FHA domain-containing protein weak similarity
to SP|Q28147 Nuclear inhibitor of protein phosphatase-1
(NIPP-1) (Protein phosphatase 1, regulatory inhibitor
subunit 8) {Bos taurus}; contains Pfam profile PF00498:
FHA domain
Length = 314
Score = 31.5 bits (68), Expect = 0.41
Identities = 19/58 (32%), Positives = 26/58 (44%), Gaps = 1/58 (1%)
Frame = -1
Query: 259 ERRNQREVKEKKSKANMRKTTKGKRTTADPPNIQREK-ERVWKNQKKGTASSRLSPSR 89
E REV +K+ ++ T K +RT D R + ER + SSR SP R
Sbjct: 60 EGERDREVGDKRRRSGREDTEKRRRTRTDDERYSRGRHERSTSPSDRSHRSSRRSPER 117
>At1g56660.1 68414.m06516 expressed protein
Length = 522
Score = 31.1 bits (67), Expect = 0.54
Identities = 25/108 (23%), Positives = 48/108 (44%), Gaps = 1/108 (0%)
Frame = -1
Query: 466 EGLTLEKEGYKSDYDRNEYEERGSEHQEDNDSDXXXXXXXXXXXXXXXXXXXERRTHDKF 287
E L EKEG K ++ E +E G E +++ +D ++ K
Sbjct: 124 EELEEEKEGKKKK-NKKEKDESGPE-EKNKKADKEKKHEDVSQEKEELEEEDGKKNKKKE 181
Query: 286 SIGKNGISAERRNQREVKEKK-SKANMRKTTKGKRTTADPPNIQREKE 146
+++ ++E K+K+ SK+N K KGK+ + ++++E E
Sbjct: 182 KDESGTEEKKKKPKKEKKQKEESKSNEDKKVKGKKEKGEKGDLEKEDE 229
>At1g20920.1 68414.m02619 DEAD box RNA helicase, putative similar to
RNA helicase [Rattus norvegicus] GI:897915; contains
Pfam profiles PF00270: DEAD/DEAH box helicase, PF00271:
Helicase conserved C-terminal domain
Length = 1166
Score = 31.1 bits (67), Expect = 0.54
Identities = 28/110 (25%), Positives = 50/110 (45%)
Frame = -1
Query: 451 EKEGYKSDYDRNEYEERGSEHQEDNDSDXXXXXXXXXXXXXXXXXXXERRTHDKFSIGKN 272
E+E + D DR +R SE ++ +DS+ R H++ GK
Sbjct: 78 ERERRRRDKDR---VKRRSERRKSSDSEDDVEEEDERDKRRVNEKERGHREHER-DRGK- 132
Query: 271 GISAERRNQREVKEKKSKANMRKTTKGKRTTADPPNIQREKERVWKNQKK 122
+R+ RE +E+K K R+ + +R + +REKERV + +++
Sbjct: 133 ----DRKRDREREERKDKEREREKDRERR---EREREEREKERVKERERR 175
>At5g60530.1 68418.m07590 late embryogenesis abundant
protein-related / LEA protein-related similar to late
embryogenesis abundant protein [Picea glauca] GI:1350543
Length = 439
Score = 30.3 bits (65), Expect = 0.94
Identities = 19/57 (33%), Positives = 27/57 (47%)
Frame = -1
Query: 295 DKFSIGKNGISAERRNQREVKEKKSKANMRKTTKGKRTTADPPNIQREKERVWKNQK 125
DK K+ E++++ E KEKK K K K K ++EKER K +K
Sbjct: 67 DKEKAAKDKKEKEKKDKEE-KEKKDKERKEKEKKDKLEKEKKDKERKEKERKEKERK 122
>At5g55920.1 68418.m06975 nucleolar protein, putative similar to
SP|P46087 Proliferating-cell nucleolar antigen p120
(Proliferation-associated nucleolar protein p120) {Homo
sapiens}, SP|P40991 Nucleolar protein NOP2
{Saccharomyces cerevisiae}; contains Pfam profile
PF01189: NOL1/NOP2/sun family
Length = 682
Score = 29.1 bits (62), Expect = 2.2
Identities = 13/47 (27%), Positives = 25/47 (53%), Gaps = 3/47 (6%)
Frame = -1
Query: 259 ERRNQREVKEKKSKANMRKTTKGKRTTADPPNI---QREKERVWKNQ 128
E++N+ ++ + K +K K K + P+ Q++K R WKN+
Sbjct: 616 EKKNKEKLAKSKEDKRGKKDKKSKSENVEEPSKPRKQKKKRREWKNE 662
>At4g33390.1 68417.m04746 hypothetical protein contains Pfam profile
PF05701: Plant protein of unknown function (DUF827)
Length = 779
Score = 29.1 bits (62), Expect = 2.2
Identities = 17/52 (32%), Positives = 26/52 (50%), Gaps = 2/52 (3%)
Frame = -1
Query: 295 DKFSIGKNGISAERRNQREVKEKKSK--ANMRKTTKGKRTTADPPNIQREKE 146
+K GK G+ E R REV EKK K ++ K+ +G + ++ E E
Sbjct: 697 EKAKEGKLGVEQELRKWREVSEKKRKNGSSHGKSIQGSKEKEAETSVSNETE 748
>At1g31460.1 68414.m03852 expressed protein
Length = 301
Score = 29.1 bits (62), Expect = 2.2
Identities = 22/94 (23%), Positives = 42/94 (44%), Gaps = 1/94 (1%)
Frame = -1
Query: 295 DKFSIGKNGISAERRNQREVKEKKSKANM-RKTTKGKRTTADPPNIQREKERVWKNQKKG 119
D +I ++ I + + Q + K K K + ++ A PP+ E E+ K + K
Sbjct: 140 DSGNISESKILSPVKQQTKKKLPKEKRRIVSPSSSSSIDLATPPSTDSEPEKKSKPKSKS 199
Query: 118 TASSRLSPSRWVNPA*RSRIIQNRIPTRKSRNWK 17
+ +LSPS+ + RS ++ + +N K
Sbjct: 200 SWFDKLSPSKLIGSIWRSSPKKSTTNKKNLKNCK 233
>At5g48610.1 68418.m06012 expressed protein ; expression supported
by MPSS
Length = 470
Score = 28.7 bits (61), Expect = 2.9
Identities = 16/45 (35%), Positives = 25/45 (55%)
Frame = -1
Query: 253 RNQREVKEKKSKANMRKTTKGKRTTADPPNIQREKERVWKNQKKG 119
+ +RE KEKKSK + K ++ D Q++KE K ++KG
Sbjct: 24 KEKREGKEKKSKDRSKDKQKERKEKKDKHKDQKDKE---KGKEKG 65
>At3g29075.1 68416.m03637 glycine-rich protein
Length = 294
Score = 28.7 bits (61), Expect = 2.9
Identities = 10/28 (35%), Positives = 19/28 (67%)
Frame = -1
Query: 451 EKEGYKSDYDRNEYEERGSEHQEDNDSD 368
+KE YK +D ++Y+E+ + ++ ND D
Sbjct: 189 KKEQYKEHHDDDDYDEKKKKKKDYNDDD 216
>At1g48970.1 68414.m05489 eukaryotic translation initiation factor
2B family protein / eIF-2B family protein similar to
guanine nucleotide exchange factor, eIF-2B, delta
subunit [Mus musculus] GI:529428; contains Pfam profile
PF01008: Initiation factor 2 subunit family
Length = 756
Score = 28.7 bits (61), Expect = 2.9
Identities = 21/62 (33%), Positives = 27/62 (43%)
Frame = -1
Query: 262 AERRNQREVKEKKSKANMRKTTKGKRTTADPPNIQREKERVWKNQKKGTASSRLSPSRWV 83
+E + KEK+ KA KTTK +R +E +R K KG S R S
Sbjct: 275 SESATTKPQKEKEPKALKDKTTKAERRAI------QEAQRAAKAAAKGEGSRRADESGRA 328
Query: 82 NP 77
NP
Sbjct: 329 NP 330
>At3g09620.1 68416.m01141 DEAD/DEAH box helicase, putative similar
to RNA helicase GB:A57514 GI:897915 from [Rattus
norvegicus]; contains Pfam profiles PF00270: DEAD/DEAH
box helicase, PF00271: Helicase conserved C-terminal
domain
Length = 989
Score = 28.3 bits (60), Expect = 3.8
Identities = 16/46 (34%), Positives = 23/46 (50%)
Frame = -1
Query: 259 ERRNQREVKEKKSKANMRKTTKGKRTTADPPNIQREKERVWKNQKK 122
ER ++R ++K R +GK D +REKE+ K QKK
Sbjct: 63 ERDSKRRGEDKGRGRRERDRDRGKYLKRDRERREREKEKGRKKQKK 108
>At1g62970.1 68414.m07110 DNAJ heat shock N-terminal
domain-containing protein low similarity to AHM1
[Triticum aestivum] GI:6691467; contains Pfam profile
PF00226: DnaJ domain
Length = 797
Score = 27.9 bits (59), Expect = 5.0
Identities = 12/32 (37%), Positives = 18/32 (56%)
Frame = +1
Query: 283 WRTCRGSYVLHTFPLHRIFRIRNLCCMSRRCR 378
W TC YVL+ +P+ I+ L C ++ CR
Sbjct: 646 WTTCPYCYVLYEYPI--IYEESVLKCQTKSCR 675
>At5g09880.1 68418.m01142 RNA recognition motif (RRM)-containing
protein
Length = 527
Score = 27.5 bits (58), Expect = 6.6
Identities = 25/99 (25%), Positives = 38/99 (38%)
Frame = -1
Query: 451 EKEGYKSDYDRNEYEERGSEHQEDNDSDXXXXXXXXXXXXXXXXXXXERRTHDKFSIGKN 272
E+E ++S D++ ++ E D +SD ERR +K S
Sbjct: 76 ERERHRSSRDKDRERDKVREGSRDKESDRERSSKERDRSDRDKPRDRERREREKRS---- 131
Query: 271 GISAERRNQREVKEKKSKANMRKTTKGKRTTADPPNIQR 155
S RR ++E E + + R K ADP QR
Sbjct: 132 -SSRSRREEKE-PEVVERGSRRHRDKKDEPEADPERDQR 168
>At3g28110.1 68416.m03508 hypothetical protein
Length = 354
Score = 27.5 bits (58), Expect = 6.6
Identities = 11/31 (35%), Positives = 18/31 (58%)
Frame = -1
Query: 466 EGLTLEKEGYKSDYDRNEYEERGSEHQEDND 374
E + +++E + D NEYE+ +E ED D
Sbjct: 132 EDINVDEEDDIDEGDENEYEDEDNEEDEDMD 162
>At2g30840.1 68415.m03760 2-oxoglutarate-dependent dioxygenase,
putative similar to 2A6 (GI:599622) and tomato ethylene
synthesis regulatory protein E8 (SP|P10967)
Length = 362
Score = 27.5 bits (58), Expect = 6.6
Identities = 10/17 (58%), Positives = 12/17 (70%)
Frame = +1
Query: 58 LSCFFKPDLPNEKDLDD 108
LSCF PD+P +DL D
Sbjct: 153 LSCFMAPDVPETEDLPD 169
>At2g16140.1 68415.m01850 expressed protein contains similarity to
hypothetical proteins
Length = 311
Score = 27.1 bits (57), Expect = 8.7
Identities = 13/46 (28%), Positives = 21/46 (45%)
Frame = -1
Query: 259 ERRNQREVKEKKSKANMRKTTKGKRTTADPPNIQREKERVWKNQKK 122
E R + K +KA +KT T D N E + +W+ ++K
Sbjct: 210 EEAMSRPIGVKAAKAKAKKTVTKTTTVEDKGNAMLEIQSIWEIKQK 255
>At1g65190.1 68414.m07391 protein kinase family protein contains
similarity to protein kinases
Length = 396
Score = 27.1 bits (57), Expect = 8.7
Identities = 14/32 (43%), Positives = 20/32 (62%)
Frame = -1
Query: 463 GLTLEKEGYKSDYDRNEYEERGSEHQEDNDSD 368
GLTL GYKS ++ EE+ SE ++ D+D
Sbjct: 238 GLTLLL-GYKSYFEHYRGEEKESEEEDPEDTD 268
>At1g10320.1 68414.m01162 U2 snRNP auxiliary factor-related similar
to U2 small nuclear ribonucleoprotein auxiliary factor
35 kD subunit related protein 1 (sp|Q15695)
Length = 757
Score = 27.1 bits (57), Expect = 8.7
Identities = 18/92 (19%), Positives = 36/92 (39%)
Frame = -1
Query: 304 RTHDKFSIGKNGISAERRNQREVKEKKSKANMRKTTKGKRTTADPPNIQREKERVWKNQK 125
R HD ++G R+ + ++ + +R+ + D P+ ER ++
Sbjct: 469 RDHDHANVGSTPSYRSRKYHGDTQDSTREDKLRRHAENCHDGDDSPSRDGSLEREMYKER 528
Query: 124 KGTASSRLSPSRWVNPA*RSRIIQNRIPTRKS 29
+ + SRW + R+ + RI R S
Sbjct: 529 RYAKDTLHRDSRWSEHSPGHRVGRKRIHGRYS 560
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 7,447,717
Number of Sequences: 28952
Number of extensions: 110435
Number of successful extensions: 624
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 553
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 604
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1092379416
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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