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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= msgV0030.Seq
         (329 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g51680.2 68414.m05823 4-coumarate--CoA ligase 1 / 4-coumaroyl...    29   0.75 
At1g51680.1 68414.m05822 4-coumarate--CoA ligase 1 / 4-coumaroyl...    29   0.75 
At3g06670.1 68416.m00786 expressed protein                             28   1.7  
At5g39210.1 68418.m04749 expressed protein                             26   5.3  
At5g18530.1 68418.m02191 beige/BEACH domain-containing protein c...    26   5.3  
At5g39460.1 68418.m04779 F-box family protein contains Pfam:PF00...    26   7.0  
At3g53570.3 68416.m05915 protein kinase (AFC1) (AME2) identical ...    26   7.0  
At3g53570.2 68416.m05916 protein kinase (AFC1) (AME2) identical ...    26   7.0  
At3g53570.1 68416.m05917 protein kinase (AFC1) (AME2) identical ...    26   7.0  
At2g40980.1 68415.m05062 expressed protein                             26   7.0  

>At1g51680.2 68414.m05823 4-coumarate--CoA ligase 1 /
           4-coumaroyl-CoA synthase 1 (4CL1) identical to SP|Q42524
           4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1)
           (4-coumaroyl-CoA synthase 1) {Arabidopsis thaliana}
          Length = 490

 Score = 29.1 bits (62), Expect = 0.75
 Identities = 15/42 (35%), Positives = 24/42 (57%), Gaps = 1/42 (2%)
 Frame = +1

Query: 136 GVLKTCSDDNVALCLKEKALRYVENVSXSXELN-LIDGVSLS 258
           GV+  C DDN ++ + E  LR+ E    + E + +ID V +S
Sbjct: 159 GVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVEIS 200


>At1g51680.1 68414.m05822 4-coumarate--CoA ligase 1 /
           4-coumaroyl-CoA synthase 1 (4CL1) identical to SP|Q42524
           4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1)
           (4-coumaroyl-CoA synthase 1) {Arabidopsis thaliana}
          Length = 561

 Score = 29.1 bits (62), Expect = 0.75
 Identities = 15/42 (35%), Positives = 24/42 (57%), Gaps = 1/42 (2%)
 Frame = +1

Query: 136 GVLKTCSDDNVALCLKEKALRYVENVSXSXELN-LIDGVSLS 258
           GV+  C DDN ++ + E  LR+ E    + E + +ID V +S
Sbjct: 159 GVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVEIS 200


>At3g06670.1 68416.m00786 expressed protein
          Length = 865

 Score = 27.9 bits (59), Expect = 1.7
 Identities = 12/21 (57%), Positives = 15/21 (71%), Gaps = 1/21 (4%)
 Frame = +1

Query: 109 DEDVFRSVMGVLKTCSD-DNV 168
           D D FR++MGV K C D +NV
Sbjct: 167 DHDFFRNLMGVFKICEDLENV 187


>At5g39210.1 68418.m04749 expressed protein
          Length = 156

 Score = 26.2 bits (55), Expect = 5.3
 Identities = 13/30 (43%), Positives = 17/30 (56%)
 Frame = -1

Query: 227 SXEXETFSTYRKAFSLRHNATLSSEQVFST 138
           S + + F+   K FS RHN  +S EQV  T
Sbjct: 4   SLQKQLFNNGDKLFSSRHNRRVSIEQVHVT 33


>At5g18530.1 68418.m02191 beige/BEACH domain-containing protein
           contains 5 WD-40 repeats (PF00400); contains
           Beige/BEACH domain (Pfam PF02138);  FACTOR ASSOCIATED
           WITH N-SMASE ACTIVATION (FAN) (SP:Q92636) Homo
           sapiens;similar to Lipopolysaccharide-responsive and
           beige-like anchor protein (CDC4-like protein)
           (Beige-like protein) (SP:P50851) [Homo sapiens}
          Length = 909

 Score = 26.2 bits (55), Expect = 5.3
 Identities = 13/30 (43%), Positives = 17/30 (56%)
 Frame = -2

Query: 145 SALPSLIGRRLRHYSLRPQRILLTPPGASW 56
           SAL  L G ++ H  +RP  ILL+    SW
Sbjct: 270 SALAHLHGLKVSHGDIRPSNILLSDSLWSW 299


>At5g39460.1 68418.m04779 F-box family protein contains Pfam:PF00646
           F-box domain ; similar to SKP1 interacting partner 2
           (SKIP2) TIGR_Ath1:At5g67250
          Length = 571

 Score = 25.8 bits (54), Expect = 7.0
 Identities = 11/33 (33%), Positives = 20/33 (60%)
 Frame = +1

Query: 208 NVSXSXELNLIDGVSLSVKAXLDQPGPXSLYPT 306
           ++  S  +NL+D V+  V   +D+P   +L+PT
Sbjct: 206 DLDFSDRMNLLDIVTKHVSLRVDEPLTGNLFPT 238


>At3g53570.3 68416.m05915 protein kinase (AFC1) (AME2) identical to
           protein kinase AFC1 (EC 2.7.1.-) [Arabidopsis thaliana]
          Length = 453

 Score = 25.8 bits (54), Expect = 7.0
 Identities = 13/37 (35%), Positives = 19/37 (51%)
 Frame = -2

Query: 187 FP*DTMRRCHRSKFSALPSLIGRRLRHYSLRPQRILL 77
           FP D +R   R    ++  +   RL H  L+P+ ILL
Sbjct: 198 FPIDLVRELGRQLLESVAYMHDLRLIHTDLKPENILL 234


>At3g53570.2 68416.m05916 protein kinase (AFC1) (AME2) identical to
           protein kinase AFC1 (EC 2.7.1.-) [Arabidopsis thaliana]
          Length = 467

 Score = 25.8 bits (54), Expect = 7.0
 Identities = 13/37 (35%), Positives = 19/37 (51%)
 Frame = -2

Query: 187 FP*DTMRRCHRSKFSALPSLIGRRLRHYSLRPQRILL 77
           FP D +R   R    ++  +   RL H  L+P+ ILL
Sbjct: 212 FPIDLVRELGRQLLESVAYMHDLRLIHTDLKPENILL 248


>At3g53570.1 68416.m05917 protein kinase (AFC1) (AME2) identical to
           protein kinase AFC1 (EC 2.7.1.-) [Arabidopsis thaliana]
          Length = 467

 Score = 25.8 bits (54), Expect = 7.0
 Identities = 13/37 (35%), Positives = 19/37 (51%)
 Frame = -2

Query: 187 FP*DTMRRCHRSKFSALPSLIGRRLRHYSLRPQRILL 77
           FP D +R   R    ++  +   RL H  L+P+ ILL
Sbjct: 212 FPIDLVRELGRQLLESVAYMHDLRLIHTDLKPENILL 248


>At2g40980.1 68415.m05062 expressed protein 
          Length = 617

 Score = 25.8 bits (54), Expect = 7.0
 Identities = 13/40 (32%), Positives = 20/40 (50%)
 Frame = -2

Query: 184 P*DTMRRCHRSKFSALPSLIGRRLRHYSLRPQRILLTPPG 65
           P + +R CH    SAL S     +RH  +RP+ ++    G
Sbjct: 445 PEEAIRCCHDC-LSALSSSSSAGIRHGDIRPENVVYVTSG 483


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.312    0.131    0.364 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 6,555,812
Number of Sequences: 28952
Number of extensions: 105051
Number of successful extensions: 155
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 154
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 155
length of database: 12,070,560
effective HSP length: 71
effective length of database: 10,014,968
effective search space used: 380568784
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.2 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (21.8 bits)

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