Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0023.Seq
(379 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g34670.1 68417.m04922 40S ribosomal protein S3A (RPS3aB) 84 3e-17
At3g04840.1 68416.m00525 40S ribosomal protein S3A (RPS3aA) simi... 83 4e-17
At1g78700.1 68414.m09173 brassinosteroid signalling positive reg... 27 3.1
At5g38550.1 68418.m04661 jacalin lectin family protein similar t... 27 4.1
At5g44290.1 68418.m05421 protein kinase family protein contains ... 27 5.5
At1g54570.1 68414.m06223 esterase/lipase/thioesterase family pro... 27 5.5
At5g28080.1 68418.m03391 protein kinase family protein contains ... 26 7.2
At4g36390.1 68417.m05170 radical SAM domain-containing protein /... 26 7.2
At1g79830.1 68414.m09326 expressed protein weak similarity to TA... 26 7.2
At1g60130.1 68414.m06774 jacalin lectin family protein similar t... 26 9.6
At3g17630.1 68416.m02252 cation/hydrogen exchanger, putative (CH... 23 9.9
>At4g34670.1 68417.m04922 40S ribosomal protein S3A (RPS3aB)
Length = 262
Score = 84.2 bits (199), Expect = 3e-17
Identities = 43/96 (44%), Positives = 63/96 (65%), Gaps = 2/96 (2%)
Frame = -3
Query: 371 CIGFTNKDSLSQRKTCYGPAHSGQSNQKENV*NHYTRRHNS--ELREVVNKLIPDSIAKD 198
CI FT + + ++TCY A S Q Q + + S +L+E+V K IP++I ++
Sbjct: 139 CIAFTKRRANQVKRTCY--AQSSQIRQIRRKMSEIMVKEASSCDLKELVAKFIPEAIGRE 196
Query: 197 IEKACHGIYPLRDVCIRKVKVLKRPRFEISKLMELH 90
IEKA GIYPL++V IRKVK+LK P+F++ KLME+H
Sbjct: 197 IEKATQGIYPLQNVFIRKVKILKAPKFDLGKLMEVH 232
>At3g04840.1 68416.m00525 40S ribosomal protein S3A (RPS3aA) similar
to 40S ribosomal protein S3A (S phase specific protein
GBIS289) GB:P49396 [Brassica rapa]
Length = 262
Score = 83.4 bits (197), Expect = 4e-17
Identities = 42/96 (43%), Positives = 66/96 (68%), Gaps = 2/96 (2%)
Frame = -3
Query: 371 CIGFTNKDSLSQRKTCYGPAHSGQSNQ-KENV*NHYTRRHNS-ELREVVNKLIPDSIAKD 198
CI FT + + ++TCY A S Q Q + + + R +S +L+++V K IP++I ++
Sbjct: 139 CIAFTKRRANQVKRTCY--AQSSQIRQIRRKMRDIMVREASSCDLKDLVAKFIPEAIGRE 196
Query: 197 IEKACHGIYPLRDVCIRKVKVLKRPRFEISKLMELH 90
IEKA GIYPL++V IRKVK+LK P+F++ KLM++H
Sbjct: 197 IEKATQGIYPLQNVFIRKVKILKAPKFDLGKLMDVH 232
>At1g78700.1 68414.m09173 brassinosteroid signalling positive
regulator-related contains similarity to BZR1 protein
[Arabidopsis thaliana] gi|20270971|gb|AAM18490
Length = 325
Score = 27.5 bits (58), Expect = 3.1
Identities = 17/48 (35%), Positives = 21/48 (43%), Gaps = 3/48 (6%)
Frame = +2
Query: 140 SPFGCKHRAEGRCHGRPSRCPWQWSQESTCSP--PP*V-QSCDVACND 274
+PFG K A G W Q TCSP PP Q+ DV ++
Sbjct: 234 NPFGFKEEAASAAGGGGGSRMWTPGQSGTCSPAIPPGADQTADVPMSE 281
>At5g38550.1 68418.m04661 jacalin lectin family protein similar to
myrosinase-binding protein homolog [Arabidopsis
thaliana] GI:2997767; contains Pfam profile PF01419
jacalin-like lectin domain
Length = 594
Score = 27.1 bits (57), Expect = 4.1
Identities = 9/12 (75%), Positives = 10/12 (83%)
Frame = -2
Query: 213 LHCQGHREGLPW 178
L CQG R+GLPW
Sbjct: 299 LECQGDRKGLPW 310
>At5g44290.1 68418.m05421 protein kinase family protein contains
Pfam domain, PF00069: Protein kinase domain
Length = 644
Score = 26.6 bits (56), Expect = 5.5
Identities = 11/25 (44%), Positives = 16/25 (64%)
Frame = -3
Query: 176 IYPLRDVCIRKVKVLKRPRFEISKL 102
+Y RD+ K+ LKR RF++S L
Sbjct: 151 VYKARDLTNNKIVALKRVRFDLSDL 175
>At1g54570.1 68414.m06223 esterase/lipase/thioesterase family
protein contains Interpro entry IPR000379
Length = 704
Score = 26.6 bits (56), Expect = 5.5
Identities = 12/39 (30%), Positives = 18/39 (46%)
Frame = +3
Query: 54 VSGFPLASTAFAVKFHQLRDLETGPLQHFHLSDANIAQR 170
++G P V +H L LE GP+ + + NI R
Sbjct: 438 LAGVPDKGPVLLVGYHMLMGLELGPMSEAFIKEKNILFR 476
>At5g28080.1 68418.m03391 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 406
Score = 26.2 bits (55), Expect = 7.2
Identities = 12/38 (31%), Positives = 22/38 (57%)
Frame = +3
Query: 135 HFHLSDANIAQRVDAMAGLLDVLGNGVRNQLVHHLPEF 248
HF D + +RV++ GL+D G +R+ +H+P +
Sbjct: 194 HFLCIDESDMRRVESEKGLIDEAGTPLRHS--YHIPHY 229
>At4g36390.1 68417.m05170 radical SAM domain-containing protein /
TRAM domain-containing protein similar to CDK5
activator-binding protein [Rattus norvegicus]
GI:7330738; contains Pfam profiles PF00919: UPF0004
family protein, PF01938: TRAM domain, PF04055: radical
SAM domain protein
Length = 640
Score = 26.2 bits (55), Expect = 7.2
Identities = 13/35 (37%), Positives = 18/35 (51%)
Frame = -3
Query: 317 PAHSGQSNQKENV*NHYTRRHNSELREVVNKLIPD 213
PA SG S E + YTR +L + + +IPD
Sbjct: 424 PAQSGNSRILEQMRRGYTREAYLDLVKKIRSIIPD 458
>At1g79830.1 68414.m09326 expressed protein weak similarity to TATA
element modulatory factor (TMF) (Swiss-Prot:P82094)
[Homo sapiens]
Length = 927
Score = 26.2 bits (55), Expect = 7.2
Identities = 15/39 (38%), Positives = 23/39 (58%)
Frame = -3
Query: 305 GQSNQKENV*NHYTRRHNSELREVVNKLIPDSIAKDIEK 189
G++NQ E R+H EL+EV+ L + I KD+E+
Sbjct: 727 GRANQLEVEIRELRRKHKQELQEVL--LHNELIQKDLER 763
>At1g60130.1 68414.m06774 jacalin lectin family protein similar to
myrosinase-binding protein homolog [Arabidopsis
thaliana] GI:2997767; contains Pfam profile PF01419
jacalin-like lectin domain
Length = 600
Score = 25.8 bits (54), Expect = 9.6
Identities = 10/21 (47%), Positives = 12/21 (57%)
Frame = -3
Query: 371 CIGFTNKDSLSQRKTCYGPAH 309
CI + D + RK YGPAH
Sbjct: 195 CIQYIKFDYVKDRKYIYGPAH 215
>At3g17630.1 68416.m02252 cation/hydrogen exchanger, putative
(CHX19) similar to putative Na+/H+-exchanging protein
GB:CAA23036 from [Arabidopsis thaliana]; monovalent
cation:proton antiporter family 2 (CPA2) member,
PMID:11500563
Length = 857
Score = 23.0 bits (47), Expect(2) = 9.9
Identities = 16/54 (29%), Positives = 24/54 (44%), Gaps = 1/54 (1%)
Frame = +3
Query: 99 HQLRDLETGPLQH-FHLSDANIAQRVDAMAGLLDVLGNGVRNQLVHHLPEFRVV 257
HQ D + H FH + + QR G+L G G +Q+V ++VV
Sbjct: 631 HQRMDGAMESIGHRFHEVNQRVLQRAPCSVGILVDRGLGGTSQVVASEVAYKVV 684
Score = 21.0 bits (42), Expect(2) = 9.9
Identities = 10/29 (34%), Positives = 12/29 (41%)
Frame = +3
Query: 24 RLVALRPFRLVSGFPLASTAFAVKFHQLR 110
R VA+RP +SG HQ R
Sbjct: 592 RAVAVRPMTAISGLSSIHEDICTSAHQKR 620
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 7,631,898
Number of Sequences: 28952
Number of extensions: 146871
Number of successful extensions: 464
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 449
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 460
length of database: 12,070,560
effective HSP length: 73
effective length of database: 9,957,064
effective search space used: 517767328
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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