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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= mg--0040
         (740 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S...   124   6e-29
At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) str...   124   6e-29
At5g17650.1 68418.m02069 glycine/proline-rich protein glycine/pr...    31   0.80 
At2g47550.1 68415.m05934 pectinesterase family protein contains ...    30   1.4  
At2g43680.2 68415.m05430 calmodulin-binding family protein simil...    29   3.2  
At2g43680.1 68415.m05429 calmodulin-binding family protein simil...    29   3.2  
At1g53120.1 68414.m06015 RNA-binding S4 domain-containing protein      29   4.3  
At1g26400.1 68414.m03220 FAD-binding domain-containing protein s...    28   5.7  
At5g07520.1 68418.m00861 glycine-rich protein (GRP18) Oleosin; g...    27   9.9  
At3g60160.1 68416.m06717 ABC transporter family protein similar ...    27   9.9  
At1g18670.1 68414.m02330 protein kinase family protein contains ...    27   9.9  

>At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S
           roibosomal protein L4, Arabidopsis thaliana,
           EMBL:CAA79104
          Length = 407

 Score =  124 bits (299), Expect = 6e-29
 Identities = 53/59 (89%), Positives = 56/59 (94%)
 Frame = +3

Query: 3   VSKEAGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWH 179
           VSK+AGHQTSAESWGTGRAV+RIPRV GGGTHR+GQ AFGNMCRGGRMFAPTK WRRWH
Sbjct: 60  VSKKAGHQTSAESWGTGRAVSRIPRVPGGGTHRAGQAAFGNMCRGGRMFAPTKIWRRWH 118



 Score = 91.9 bits (218), Expect = 4e-19
 Identities = 40/87 (45%), Positives = 59/87 (67%)
 Frame = +1

Query: 478 QGSGSDSRFPHIPGVEXXXXXXXXXXXXAPGGHLGRFVIWTQSAFGRLDPLFGSWKTPSK 657
           +G+     F ++PGVE            APGGHLGRFVIWT+SAF +L+ ++GS++ PS+
Sbjct: 220 EGAKIVKAFRNLPGVELCHVERLNLLKLAPGGHLGRFVIWTKSAFEKLESIYGSFEKPSE 279

Query: 658 QKKNFNLPQPKMANTDLTRLLKSDEIR 738
           +KK + LP+ KM N DL R++ SDE++
Sbjct: 280 KKKGYVLPRAKMVNADLARIINSDEVQ 306



 Score = 89.4 bits (212), Expect = 2e-18
 Identities = 42/88 (47%), Positives = 58/88 (65%), Gaps = 1/88 (1%)
 Frame = +2

Query: 257 ARGHIIEKIPELPLVVADKVQEINKTKQAVIFLRRLKAWSDILKVYKSQRLRAGKGKMRN 436
           ARGH IE +PE+PLVV+D  + + KT  A+  L+++ A+ D  K   S  +R GKGKMRN
Sbjct: 145 ARGHKIENVPEMPLVVSDSAEAVEKTSAAIKVLKQIGAYDDAEKAKNSIGIRPGKGKMRN 204

Query: 437 RRRIQRKGPLIIFNKDQG-LTRAFRTSP 517
           RR I RKGPL++F  +   + +AFR  P
Sbjct: 205 RRYISRKGPLVVFGTEGAKIVKAFRNLP 232


>At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) strong
           similarity to 60S ribosomal protein L1 GB:P49691
          Length = 406

 Score =  124 bits (299), Expect = 6e-29
 Identities = 53/59 (89%), Positives = 56/59 (94%)
 Frame = +3

Query: 3   VSKEAGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWH 179
           VSK+AGHQTSAESWGTGRAV+RIPRV GGGTHR+GQ AFGNMCRGGRMFAPTK WRRWH
Sbjct: 59  VSKKAGHQTSAESWGTGRAVSRIPRVPGGGTHRAGQAAFGNMCRGGRMFAPTKIWRRWH 117



 Score = 93.5 bits (222), Expect = 1e-19
 Identities = 42/87 (48%), Positives = 59/87 (67%)
 Frame = +1

Query: 478 QGSGSDSRFPHIPGVEXXXXXXXXXXXXAPGGHLGRFVIWTQSAFGRLDPLFGSWKTPSK 657
           +GS     F ++PGVE            APGGHLGRFVIWT+SAF +L+ ++GS++ PS+
Sbjct: 219 EGSKIVKAFRNLPGVELCHVERLNLLKLAPGGHLGRFVIWTKSAFEKLESIYGSFEKPSE 278

Query: 658 QKKNFNLPQPKMANTDLTRLLKSDEIR 738
           +KK + LP+ KM N DL R++ SDEI+
Sbjct: 279 KKKGYVLPRAKMVNADLARIINSDEIQ 305



 Score = 88.2 bits (209), Expect = 5e-18
 Identities = 41/88 (46%), Positives = 58/88 (65%), Gaps = 1/88 (1%)
 Frame = +2

Query: 257 ARGHIIEKIPELPLVVADKVQEINKTKQAVIFLRRLKAWSDILKVYKSQRLRAGKGKMRN 436
           ARGH IE +PE+PLVV+D  + + KT  A+  L+++ A+ D  K   S  +R GKGKMRN
Sbjct: 144 ARGHKIENVPEMPLVVSDSAEAVEKTSAAIKVLKQIGAYDDAEKAKNSIGIRPGKGKMRN 203

Query: 437 RRRIQRKGPLIIFNKD-QGLTRAFRTSP 517
           RR I RKGPL+++  +   + +AFR  P
Sbjct: 204 RRYISRKGPLVVYGTEGSKIVKAFRNLP 231


>At5g17650.1 68418.m02069 glycine/proline-rich protein
           glycine/proline-rich protein GPRP - Arabidopsis
           thaliana, EMBL:X84315
          Length = 173

 Score = 31.1 bits (67), Expect = 0.80
 Identities = 11/24 (45%), Positives = 13/24 (54%)
 Frame = -3

Query: 138 HHDTCYRRHPDRTYGYHHHGHAEF 67
           HH   Y  H    YGY +HGH +F
Sbjct: 122 HHHGHYGHHHGHGYGYGYHGHGKF 145


>At2g47550.1 68415.m05934 pectinesterase family protein contains
           Pfam profile: PF01095 pectinesterase
          Length = 560

 Score = 30.3 bits (65), Expect = 1.4
 Identities = 24/80 (30%), Positives = 38/80 (47%), Gaps = 2/80 (2%)
 Frame = -2

Query: 502 SASQTLILVED--YEGSLTLDTTTVAHFTLTSTKTLRLVHLKDIRPCLEAPQEDDSLFGL 329
           +A  T+  +ED  +  SLT+D    +  T  STKTL L   +D+   L A   ++     
Sbjct: 92  AAKSTVGALEDCKFLASLTMDYLLSSSQTADSTKTLSLSRAEDVHTFLSAAITNEQT--C 149

Query: 328 VDLLDFVGYNQGKLGNLFNN 269
           ++ L       G  G+LFN+
Sbjct: 150 LEGLKSTASENGLSGDLFND 169


>At2g43680.2 68415.m05430 calmodulin-binding family protein similar
           to SF16 protein [Helianthus annuus] GI:560150; contains
           Pfam profile PF00612: IQ calmodulin-binding motif
          Length = 669

 Score = 29.1 bits (62), Expect = 3.2
 Identities = 13/33 (39%), Positives = 16/33 (48%)
 Frame = +2

Query: 134 WWTYVRPHEALAALAPSRQPPTAESGLGSSRCC 232
           WW +V     LA+ APS   P  +  L  SR C
Sbjct: 465 WWNWVDRQNPLASPAPSYSQPQRDFRLTPSRLC 497


>At2g43680.1 68415.m05429 calmodulin-binding family protein similar
           to SF16 protein [Helianthus annuus] GI:560150; contains
           Pfam profile PF00612: IQ calmodulin-binding motif
          Length = 668

 Score = 29.1 bits (62), Expect = 3.2
 Identities = 13/33 (39%), Positives = 16/33 (48%)
 Frame = +2

Query: 134 WWTYVRPHEALAALAPSRQPPTAESGLGSSRCC 232
           WW +V     LA+ APS   P  +  L  SR C
Sbjct: 464 WWNWVDRQNPLASPAPSYSQPQRDFRLTPSRLC 496


>At1g53120.1 68414.m06015 RNA-binding S4 domain-containing protein
          Length = 320

 Score = 28.7 bits (61), Expect = 4.3
 Identities = 15/63 (23%), Positives = 31/63 (49%)
 Frame = -2

Query: 466 EGSLTLDTTTVAHFTLTSTKTLRLVHLKDIRPCLEAPQEDDSLFGLVDLLDFVGYNQGKL 287
           E SL +D    A F ++ +K + L+  KD+R       ++ ++    D++   G  + K+
Sbjct: 242 EASLRIDAVASAGFKISRSKLVDLISSKDVRVNWATVTKNGTIVKTGDVVSVSGKGRLKI 301

Query: 286 GNL 278
           G +
Sbjct: 302 GEI 304


>At1g26400.1 68414.m03220 FAD-binding domain-containing protein
           similar to SP|P30986 reticuline oxidase precursor
           (Berberine-bridge-forming enzyme) (BBE)
           (Tetrahydroprotoberberine synthase) [Eschscholzia
           californica]; contains PF01565 FAD binding domain
          Length = 527

 Score = 28.3 bits (60), Expect = 5.7
 Identities = 13/22 (59%), Positives = 16/22 (72%)
 Frame = -3

Query: 339 CLVLLISWTLSATTKGSSGIFS 274
           CLVLL+S   +A TK  SGIF+
Sbjct: 9   CLVLLVSILRAAVTKPDSGIFT 30


>At5g07520.1 68418.m00861 glycine-rich protein (GRP18) Oleosin;
           glycine-rich protein 18 (GRP18) PMID:11431566;
          Length = 228

 Score = 27.5 bits (58), Expect = 9.9
 Identities = 15/58 (25%), Positives = 25/58 (43%), Gaps = 3/58 (5%)
 Frame = +3

Query: 3   VSKEAGHQTSAESWGTGRAVARIPRVRGGGTHRSGQ---GAFGNMCRGGRMFAPTKPW 167
           + +  G + +AE   + + + ++P   G G    G+   G FGN   GG  F     W
Sbjct: 81  IRRRMGAKPTAEGTSSAQPLLKLPVYGGYGGFWGGKKFSGTFGNKPGGGNPFGDISKW 138


>At3g60160.1 68416.m06717 ABC transporter family protein similar to
           ATP-binding cassette transporter MRP8 GI:18031899 from
           [Arabidopsis thaliana]
          Length = 1490

 Score = 27.5 bits (58), Expect = 9.9
 Identities = 21/62 (33%), Positives = 31/62 (50%)
 Frame = +2

Query: 359 RLKAWSDILKVYKSQRLRAGKGKMRNRRRIQRKGPLIIFNKDQGLTRAFRTSPVWSS*ML 538
           R+KA S+ILK  K  +L+A   +  N+ +  RK       K   L +AF T  +W +  L
Sbjct: 491 RMKATSEILKNMKILKLQAWDNQFLNKVKTLRKKEYDCLWKSLRL-QAFTTFILWGAPSL 549

Query: 539 TS 544
            S
Sbjct: 550 IS 551


>At1g18670.1 68414.m02330 protein kinase family protein contains
           Protein kinases ATP-binding region signature,
           PROSITE:PS00107 and Serine/Threonine protein kinases
           active-site signature, PROSITE:PS00108
          Length = 662

 Score = 27.5 bits (58), Expect = 9.9
 Identities = 18/56 (32%), Positives = 26/56 (46%)
 Frame = +1

Query: 244 QRSLG*RTHY*KDSRASLGCSRQSPGDQQDQTGCHLPEAPQGMV*YP*GVQVSASS 411
           Q+  G   H   DS ++L    Q P + +     H+  A QG V +   +QVS SS
Sbjct: 489 QKRNGHSVHNSIDSDSTLFEKMQKPSNHEKDEASHVKNASQGDVPFSGPLQVSVSS 544


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,168,333
Number of Sequences: 28952
Number of extensions: 336378
Number of successful extensions: 1181
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 1094
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1173
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1633819784
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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