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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= mg--0030
         (456 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g37720.1 68415.m04625 expressed protein                             29   1.5  
At4g36120.1 68417.m05141 expressed protein                             28   2.6  
At5g14570.2 68418.m01709 transporter, putative similar to trans-...    27   4.5  
At5g14570.1 68418.m01708 transporter, putative similar to trans-...    27   4.5  
At4g38640.1 68417.m05469 choline transporter-related contains we...    27   4.5  
At4g13950.1 68417.m02158 rapid alkalinization factor (RALF) fami...    27   6.0  

>At2g37720.1 68415.m04625 expressed protein 
          Length = 482

 Score = 29.1 bits (62), Expect = 1.5
 Identities = 21/73 (28%), Positives = 35/73 (47%)
 Frame = +1

Query: 31  YAYRCTGVRIPQAGTNFSNEIRTQQMFTIDFHGEGITSCNXNQTSKIIICVITGGRTSWE 210
           Y ++  G  IP+   N  N +R  Q  TI F G+ +      +  + ++C+ TGG+ S E
Sbjct: 187 YRWQPEGCNIPEF--NRVNFLRRMQNKTIAFIGDSL----GREQFQSLMCMATGGKESPE 240

Query: 211 SARVGTTTPRLFP 249
              VG+    + P
Sbjct: 241 VQNVGSEYGLVIP 253


>At4g36120.1 68417.m05141 expressed protein
          Length = 981

 Score = 28.3 bits (60), Expect = 2.6
 Identities = 10/24 (41%), Positives = 16/24 (66%)
 Frame = +3

Query: 225 YHHPPPISAVSTNAFRFEGWGSRC 296
           +H+PP +++VS + F  EG  S C
Sbjct: 432 HHYPPSVTSVSEDGFDEEGSSSEC 455


>At5g14570.2 68418.m01709 transporter, putative similar to
           trans-membrane nitrate transporter protein AtNRT2:1
           [Arabidopsis thaliana] GI:3747058, high-affinity nitrate
           transporter ACH1 [Arabidopsis thaliana] GI:3608362;
           contains Pfam profile PF00083: Sugar (and other)
           transporter
          Length = 397

 Score = 27.5 bits (58), Expect = 4.5
 Identities = 13/46 (28%), Positives = 26/46 (56%)
 Frame = -1

Query: 177 NYNFAGLIXITRCYSFTVEVNREHLLSTYFIRKIGTRLRDSNTGAS 40
           NY  A ++ +   YS+ VE+  +++++ YF  + G  L  + T A+
Sbjct: 267 NYR-AWILALLYGYSYGVELTTDNVIAGYFYERFGVNLEAAGTIAA 311


>At5g14570.1 68418.m01708 transporter, putative similar to
           trans-membrane nitrate transporter protein AtNRT2:1
           [Arabidopsis thaliana] GI:3747058, high-affinity nitrate
           transporter ACH1 [Arabidopsis thaliana] GI:3608362;
           contains Pfam profile PF00083: Sugar (and other)
           transporter
          Length = 493

 Score = 27.5 bits (58), Expect = 4.5
 Identities = 13/46 (28%), Positives = 26/46 (56%)
 Frame = -1

Query: 177 NYNFAGLIXITRCYSFTVEVNREHLLSTYFIRKIGTRLRDSNTGAS 40
           NY  A ++ +   YS+ VE+  +++++ YF  + G  L  + T A+
Sbjct: 267 NYR-AWILALLYGYSYGVELTTDNVIAGYFYERFGVNLEAAGTIAA 311


>At4g38640.1 68417.m05469 choline transporter-related contains weak
           similarity to CD92 protein [Homo sapiens]
           gi|16945323|emb|CAC82175
          Length = 556

 Score = 27.5 bits (58), Expect = 4.5
 Identities = 17/46 (36%), Positives = 23/46 (50%), Gaps = 5/46 (10%)
 Frame = -1

Query: 186 DYAN---YNFAGLIXITRCYS--FTVEVNREHLLSTYFIRKIGTRL 64
           DY N    NFA +     C S   T E+ R +LLS  F+  + TR+
Sbjct: 410 DYLNKFTINFAAITGEAYCTSAKMTYELLRRNLLSAVFVETVSTRI 455


>At4g13950.1 68417.m02158 rapid alkalinization factor (RALF) family
           protein similar to RALF precursor [Nicotiana tabacum]
           GI:16566316
          Length = 113

 Score = 27.1 bits (57), Expect = 6.0
 Identities = 23/68 (33%), Positives = 30/68 (44%)
 Frame = -1

Query: 318 VLRSQYSYNGYPTLRTETH*CSQRK*AGGGGTYPCGLPRSPTTSDYANYNFAGLIXITRC 139
           VL +Q  Y GY TLR +   C +     G   Y C   RS   + Y+     G   ITRC
Sbjct: 56  VLMAQKRYIGYETLRRDMVPCQK----PGASYYDC---RSGQANSYSR----GCDTITRC 104

Query: 138 YSFTVEVN 115
              T ++N
Sbjct: 105 ARDTNDIN 112


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,574,836
Number of Sequences: 28952
Number of extensions: 220523
Number of successful extensions: 442
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 438
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 442
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 752336160
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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