Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00020
(703 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g04840.1 68416.m00525 40S ribosomal protein S3A (RPS3aA) simi... 128 5e-30
At4g34670.1 68417.m04922 40S ribosomal protein S3A (RPS3aB) 126 1e-29
At5g44290.1 68418.m05421 protein kinase family protein contains ... 29 2.3
At3g52640.1 68416.m05799 nicastrin-related contains weak similar... 29 2.3
At2g17930.1 68415.m02076 FAT domain-containing protein / phospha... 28 6.9
>At3g04840.1 68416.m00525 40S ribosomal protein S3A (RPS3aA) similar
to 40S ribosomal protein S3A (S phase specific protein
GBIS289) GB:P49396 [Brassica rapa]
Length = 262
Score = 128 bits (308), Expect = 5e-30
Identities = 58/88 (65%), Positives = 71/88 (80%)
Frame = +3
Query: 243 FPQIRLIAEYVQGRNVLCNFHGMDLTTDKLRWMVKKWQTLIEANIDVKTTDGYVLRVFCI 422
+ +IRL AE VQGRNVLC F GMD TTDKLR +VKKWQTLIEA++DVKTTD Y LR+FCI
Sbjct: 81 YRKIRLRAEDVQGRNVLCQFWGMDFTTDKLRSLVKKWQTLIEAHVDVKTTDSYTLRLFCI 140
Query: 423 GFTNKDSLSQRKTCYAQHTQVRAIRKKM 506
FT + + ++TCYAQ +Q+R IR+KM
Sbjct: 141 AFTKRRANQVKRTCYAQSSQIRQIRRKM 168
Score = 103 bits (246), Expect = 2e-22
Identities = 49/66 (74%), Positives = 57/66 (86%)
Frame = +1
Query: 58 VDPFTRKDWYDVKAPSMFSKRQVGTTLVNRTQGTKIASEGLKGRVFEVSLADLQADTDAE 237
VDPF++KDWYDVKAPS+F+ R VG TLV+RTQGTKIASEGLK RVFEVSLADLQ D D
Sbjct: 21 VDPFSKKDWYDVKAPSIFTHRNVGKTLVSRTQGTKIASEGLKHRVFEVSLADLQGDED-- 78
Query: 238 RSFRKL 255
++RK+
Sbjct: 79 NAYRKI 84
Score = 46.0 bits (104), Expect = 2e-05
Identities = 18/32 (56%), Positives = 27/32 (84%)
Frame = +1
Query: 607 GIYPLRDVCNPKVKVLKRPRFEISKLMELHGE 702
GIYPL++V KVK+LK P+F++ KLM++HG+
Sbjct: 203 GIYPLQNVFIRKVKILKAPKFDLGKLMDVHGD 234
Score = 35.9 bits (79), Expect = 0.026
Identities = 21/66 (31%), Positives = 37/66 (56%), Gaps = 2/66 (3%)
Frame = +2
Query: 473 AHSGQSNQ--KENVEIITRDVTNSELREVVNKLIPDSIAKDIEKAAMASTLCAMFAIRK* 646
A S Q Q ++ +I+ R+ ++ +L+++V K IP++I ++IEKA IRK
Sbjct: 156 AQSSQIRQIRRKMRDIMVREASSCDLKDLVAKFIPEAIGREIEKATQGIYPLQNVFIRKV 215
Query: 647 KC*RGP 664
K + P
Sbjct: 216 KILKAP 221
>At4g34670.1 68417.m04922 40S ribosomal protein S3A (RPS3aB)
Length = 262
Score = 126 bits (304), Expect = 1e-29
Identities = 58/88 (65%), Positives = 71/88 (80%)
Frame = +3
Query: 243 FPQIRLIAEYVQGRNVLCNFHGMDLTTDKLRWMVKKWQTLIEANIDVKTTDGYVLRVFCI 422
+ +IRL AE VQGRNVL F GMD TTDKLR +VKKWQTLIEA++DVKTTDGY LR+FCI
Sbjct: 81 YRKIRLRAEDVQGRNVLTQFWGMDFTTDKLRSLVKKWQTLIEAHVDVKTTDGYTLRMFCI 140
Query: 423 GFTNKDSLSQRKTCYAQHTQVRAIRKKM 506
FT + + ++TCYAQ +Q+R IR+KM
Sbjct: 141 AFTKRRANQVKRTCYAQSSQIRQIRRKM 168
Score = 100 bits (239), Expect = 1e-21
Identities = 48/66 (72%), Positives = 55/66 (83%)
Frame = +1
Query: 58 VDPFTRKDWYDVKAPSMFSKRQVGTTLVNRTQGTKIASEGLKGRVFEVSLADLQADTDAE 237
VDPF++KDWYDVKAP F+ R VG TLV+RTQGTKIASEGLK RVFEVSLADLQ D D
Sbjct: 21 VDPFSKKDWYDVKAPGSFTNRNVGKTLVSRTQGTKIASEGLKHRVFEVSLADLQNDED-- 78
Query: 238 RSFRKL 255
++RK+
Sbjct: 79 NAYRKI 84
Score = 47.2 bits (107), Expect = 1e-05
Identities = 19/32 (59%), Positives = 27/32 (84%)
Frame = +1
Query: 607 GIYPLRDVCNPKVKVLKRPRFEISKLMELHGE 702
GIYPL++V KVK+LK P+F++ KLME+HG+
Sbjct: 203 GIYPLQNVFIRKVKILKAPKFDLGKLMEVHGD 234
Score = 37.5 bits (83), Expect = 0.009
Identities = 22/66 (33%), Positives = 37/66 (56%), Gaps = 2/66 (3%)
Frame = +2
Query: 473 AHSGQSNQ--KENVEIITRDVTNSELREVVNKLIPDSIAKDIEKAAMASTLCAMFAIRK* 646
A S Q Q ++ EI+ ++ ++ +L+E+V K IP++I ++IEKA IRK
Sbjct: 156 AQSSQIRQIRRKMSEIMVKEASSCDLKELVAKFIPEAIGREIEKATQGIYPLQNVFIRKV 215
Query: 647 KC*RGP 664
K + P
Sbjct: 216 KILKAP 221
>At5g44290.1 68418.m05421 protein kinase family protein contains
Pfam domain, PF00069: Protein kinase domain
Length = 644
Score = 29.5 bits (63), Expect = 2.3
Identities = 12/25 (48%), Positives = 17/25 (68%)
Frame = +1
Query: 610 IYPLRDVCNPKVKVLKRPRFEISKL 684
+Y RD+ N K+ LKR RF++S L
Sbjct: 151 VYKARDLTNNKIVALKRVRFDLSDL 175
>At3g52640.1 68416.m05799 nicastrin-related contains weak similarity
to Nicastrin precursor (Swiss-Prot:Q92542) [Homo
sapiens]
Length = 676
Score = 29.5 bits (63), Expect = 2.3
Identities = 16/49 (32%), Positives = 30/49 (61%)
Frame = -3
Query: 500 LSDCSDLSVLGVARLALAQGVLIGETNAEDT*NVSIGCLHINVGFDESL 354
LS+ + SV+ A + +A+ + I ++ +DT N ++G +H+N F E L
Sbjct: 440 LSNINSSSVVAAASV-VARTLYILASDNKDTSNSALGSIHVNASFVEEL 487
>At2g17930.1 68415.m02076 FAT domain-containing protein /
phosphatidylinositol 3- and 4-kinase family protein
contains Pfam profiles PF02259 FAT domain, PF00454
Phosphatidylinositol 3- and 4-kinase, PF02260: FATC
domain
Length = 3795
Score = 27.9 bits (59), Expect = 6.9
Identities = 16/49 (32%), Positives = 27/49 (55%)
Frame = -2
Query: 189 SSLQSFRSNFRSLGTVDKRGADLPLAEHRRSLDIVPIFASEWVDNLLLN 43
S LQS RS F +L + K A + ++ + L++V + S+W+ LN
Sbjct: 1576 SWLQSNRSVFDTLVLIWKSPARISRLQNEQELNLVQVKESKWLVKCFLN 1624
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,684,089
Number of Sequences: 28952
Number of extensions: 339526
Number of successful extensions: 1010
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 963
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1010
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1506636208
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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